Restriction map for
pFN21AB9616
(Linear) (Six-base) MAPSORT of: KIBB9616.seq Check: 1767 from: 1 to: 669
KIBB9616.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AvaI C'yCGr_G
Cuts at: 0 128 163 169 221 408 669
Size: 128 35 6 52 187 261
Fragments arranged by size:
261 187 128 52 35 6
BanI G'GyrC_C
Cuts at: 0 300 505 585 669
Size: 300 205 80 84
Fragments arranged by size:
300 205 84 80
BanII G_rGCy'C
Cuts at: 0 137 170 669
Size: 137 33 499
Fragments arranged by size:
499 137 33
BbeI G_GCGC'C
Cuts at: 0 304 509 589 669
Size: 304 205 80 80
Fragments arranged by size:
304 205 80 80
BglI GCCn_nnn'nGGC
Cuts at: 0 661 669
Size: 661 8
Bme1580I G_kGCm'C
Cuts at: 0 336 669
Size: 336 333
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 669
Size: 336 333
Bpu10I CC'TnA_GC
Cuts at: 0 379 669
Size: 379 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 669
Size: 310 359
BsaHI Gr'CG_yC
Cuts at: 0 301 506 586 669
Size: 301 205 80 83
Fragments arranged by size:
301 205 83 80
BsaWI w'CCGG_w
Cuts at: 0 92 276 669
Size: 92 184 393
Fragments arranged by size:
393 184 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 669
Size: 439 30 200
Fragments arranged by size:
439 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 460 669
Size: 328 132 209
Fragments arranged by size:
328 209 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 73 196 669
Size: 28 45 123 473
Fragments arranged by size:
473 123 45 28
BsiEI CG_ry'CG
Cuts at: 0 188 242 474 669
Size: 188 54 232 195
Fragments arranged by size:
232 195 188 54
BsiHKAI G_wGCw'C
Cuts at: 0 137 269 647 669
Size: 137 132 378 22
Fragments arranged by size:
378 137 132 22
Bsp1286I G_dGCh'C
Cuts at: 0 137 170 269 336 647 669
Size: 137 33 99 67 311 22
Fragments arranged by size:
311 137 99 67 33 22
BspEI T'CCGG_A
Cuts at: 0 276 669
Size: 276 393
BspHI T'CATG_A
Cuts at: 0 29 669
Size: 29 640
BsrFI r'CCGG_y
Cuts at: 0 183 669
Size: 183 486
BssSI C'ACGA_G
Cuts at: 0 646 669
Size: 646 23
BtgI C'CryG_G
Cuts at: 0 236 569 656 669
Size: 236 333 87 13
Fragments arranged by size:
333 236 87 13
EaeI y'GGCC_r
Cuts at: 0 146 185 239 669
Size: 146 39 54 430
Fragments arranged by size:
430 146 54 39
EagI C'GGCC_G
Cuts at: 0 185 239 669
Size: 185 54 430
Fragments arranged by size:
430 185 54
EarI CTCTTCn'nnn_
Cuts at: 0 437 669
Size: 437 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 312 640 669
Size: 312 328 29
Fragments arranged by size:
328 312 29
EcoICRI GAG'CTC
Cuts at: 0 135 669
Size: 135 534
EcoO109I rG'GnC_Cy
Cuts at: 0 159 669
Size: 159 510
FspI TGC'GCA
Cuts at: 0 121 669
Size: 121 548
HaeII r_GCGC'y
Cuts at: 0 128 304 509 543 589 669
Size: 128 176 205 34 46 80
Fragments arranged by size:
205 176 128 80 46 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 669
Size: 439 32 198
Fragments arranged by size:
439 198 32
KasI G'GCGC_C
Cuts at: 0 300 505 585 669
Size: 300 205 80 84
Fragments arranged by size:
300 205 84 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 669
Size: 604 65
MspA1I CmG'CkG
Cuts at: 0 105 571 669
Size: 105 466 98
Fragments arranged by size:
466 105 98
NaeI GCC'GGC
Cuts at: 0 185 669
Size: 185 484
NarI GG'CG_CC
Cuts at: 0 301 506 586 669
Size: 301 205 80 83
Fragments arranged by size:
301 205 83 80
NgoMIV G'CCGG_C
Cuts at: 0 183 669
Size: 183 486
NspI r_CATG'y
Cuts at: 0 69 636 669
Size: 69 567 33
Fragments arranged by size:
567 69 33
PspXI vC'TCGA_Gb
Cuts at: 0 163 669
Size: 163 506
PstI C_TGCA'G
Cuts at: 0 104 482 536 565 669
Size: 104 378 54 29 104
Fragments arranged by size:
378 104 104 54 29
PvuI CG_AT'CG
Cuts at: 0 474 669
Size: 474 195
PvuII CAG'CTG
Cuts at: 0 105 669
Size: 105 564
SacI G_AGCT'C
Cuts at: 0 137 669
Size: 137 532
SacII CC_GC'GG
Cuts at: 0 572 669
Size: 572 97
SfcI C'TryA_G
Cuts at: 0 100 478 532 561 669
Size: 100 378 54 29 108
Fragments arranged by size:
378 108 100 54 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 661 669
Size: 661 8
SfoI GGC'GCC
Cuts at: 0 302 507 587 669
Size: 302 205 80 82
Fragments arranged by size:
302 205 82 80
SmaI CCC'GGG
Cuts at: 0 130 669
Size: 130 539
SmlI C'TyrA_G
Cuts at: 0 163 669
Size: 163 506
SphI G_CATG'C
Cuts at: 0 69 636 669
Size: 69 567 33
Fragments arranged by size:
567 69 33
StyI C'CwwG_G
Cuts at: 0 149 669
Size: 149 520
Tth111I GACn'n_nGTC
Cuts at: 0 36 669
Size: 36 633
XhoI C'TCGA_G
Cuts at: 0 163 669
Size: 163 506
XmaI C'CCGG_G
Cuts at: 0 128 669
Size: 128 541
Enzymes that do cut:
AvaI BanI BanII BbeI BglI Bme1580I BplI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BsrFI BssSI BtgI EaeI EagI
EarI EciI EcoICRI EcoO109I FspI HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV NspI PspXI PstI
PvuI PvuII SacI SacII SfcI SfiI SfoI SmaI
SmlI SphI StyI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpmI BpuEI BsaAI BsaBI BseYI
BsiWI BsmI BsmBI BspMI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI TstI XbaI XcmI XmnI ZraI