(Circular) (Six-base) MAPSORT of: pFN21AB9615.seq Check: 3913 from: 1 to: 5497 pFN21AB9615 5497 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2197 2481 2197 Size: 284 5213 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2683 2818 2683 Size: 135 5362 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2316 2441 3560 4944 5212 1632 Size: 684 125 1119 1384 268 1917 Fragments arranged by size: 1917 1384 1119 684 268 125 AfeI AGC'GCT Cuts at: 2462 2462 Size: 5497 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2309 2711 3240 4812 2309 Size: 402 529 1572 2994 Fragments arranged by size: 2994 1572 529 402 ApaLI G'TGCA_C Cuts at: 2576 2694 3554 4710 2576 Size: 118 860 1156 3363 Fragments arranged by size: 3363 1156 860 118 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2088 2581 2822 1870 Size: 73 102 43 493 241 4545 Fragments arranged by size: 4545 493 241 102 73 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2675 2708 2675 Size: 33 5464 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2646 2683 2818 4273 618 Size: 325 217 627 859 37 135 1455 1842 Fragments arranged by size: 1842 1455 859 627 325 217 135 37 BanII G_rGCy'C Cuts at: 1391 2196 2324 2816 1391 Size: 805 128 492 4072 Fragments arranged by size: 4072 805 492 128 BbeI G_GCGC'C Cuts at: 2650 2650 Size: 5497 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2552 2586 3688 3722 1903 Size: 34 615 34 1102 34 3678 Fragments arranged by size: 3678 1102 615 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 2269 1548 Size: 721 4776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 4108 136 Size: 107 122 71 631 605 428 2008 1525 Fragments arranged by size: 2008 1525 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2283 2531 2580 2606 2698 3558 4714 1790 Size: 493 248 49 26 92 860 1156 2573 Fragments arranged by size: 2573 1156 860 493 248 92 49 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 3228 4186 Size: 416 53 83 399 24 805 1006 958 Cuts at: 4186 442 Size: 1753 Fragments arranged by size: 1753 1006 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2449 4157 1239 Size: 1172 38 1708 2579 Fragments arranged by size: 2579 1708 1172 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 3622 4487 4785 2126 Size: 1496 865 298 2838 Fragments arranged by size: 2838 1496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2296 4160 882 Size: 617 797 1864 2219 Fragments arranged by size: 2219 1864 797 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2647 3304 3686 4321 Size: 53 83 186 936 1114 657 382 635 Cuts at: 4321 275 Size: 1451 Fragments arranged by size: 1451 1114 936 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 4066 Fragments arranged by size: 4066 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2067 2116 2246 2744 2780 2783 1186 Size: 881 49 130 498 36 3 3900 Fragments arranged by size: 3900 881 498 130 49 36 3 BseYI C'CCAG_C Cuts at: 2015 2309 4700 5196 2015 Size: 294 2391 496 2316 Fragments arranged by size: 2391 2316 496 294 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 2231 1163 Size: 573 489 6 4429 Fragments arranged by size: 4429 573 489 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2796 2947 3708 4736 1540 Size: 275 186 795 151 761 1028 2301 Fragments arranged by size: 2301 1028 795 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2580 2698 3558 3643 4714 2580 Size: 118 860 85 1071 3363 Fragments arranged by size: 3363 1071 860 118 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2283 2324 2531 2580 2606 2698 Size: 399 406 87 41 207 49 26 92 Cuts at: 2698 2816 3558 3643 4714 1391 Size: 118 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 207 118 92 87 85 49 41 26 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2197 2481 2852 844 Size: 1353 284 371 3489 Fragments arranged by size: 3489 1353 371 284 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2498 2791 4141 4328 1700 Size: 110 688 293 1350 187 2869 Fragments arranged by size: 2869 1350 688 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 2442 794 Size: 1648 3849 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2701 2827 3194 3579 3596 5048 5134 5146 Size: 729 126 367 385 17 1452 86 12 Cuts at: 5146 5492 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 729 385 367 346 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2316 2316 Size: 5497 BtgI C'CryG_G Cuts at: 513 1065 2004 2676 2720 513 Size: 552 939 672 44 3290 Fragments arranged by size: 3290 939 672 552 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2555 487 Size: 683 56 1329 3429 Fragments arranged by size: 3429 1329 683 56 BtsI GCAGTG_nn' Cuts at: 2245 2482 3013 3807 3827 2245 Size: 237 531 794 20 3915 Fragments arranged by size: 3915 794 531 237 20 ClaI AT'CG_AT Cuts at: 1554 2265 1554 Size: 711 4786 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2793 2944 3833 Size: 54 1425 50 275 18 963 151 889 Cuts at: 3833 5462 8 Size: 1629 43 Fragments arranged by size: 1629 1425 963 889 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2793 2944 1537 Size: 275 981 151 4090 Fragments arranged by size: 4090 981 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2757 3427 1353 Size: 696 708 670 3423 Fragments arranged by size: 3423 708 696 670 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2411 2441 2449 3560 4157 4944 Size: 393 684 95 30 8 1111 597 787 Cuts at: 4944 5212 1239 Size: 268 1524 Fragments arranged by size: 1524 1111 787 684 597 393 268 95 30 8 EcoO109I rG'GnC_Cy Cuts at: 2338 2338 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2398 2464 2593 2650 4644 1678 Size: 720 66 129 57 1994 2531 Fragments arranged by size: 2531 1994 720 129 66 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2254 2286 2693 2725 4138 Size: 32 135 32 682 32 407 32 1413 Cuts at: 4138 4170 4212 4244 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1413 682 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KasI G'GCGC_C Cuts at: 2646 2646 Size: 5497 KpnI G_GTAC'C Cuts at: 2687 2822 2687 Size: 135 5362 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 4611 4795 1305 Size: 1104 2202 184 2007 Fragments arranged by size: 2202 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3455 3814 3973 518 Size: 648 2289 359 159 2042 Fragments arranged by size: 2289 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2306 2517 2524 2913 3590 4738 4983 1606 Size: 700 211 7 389 677 1148 245 2120 Fragments arranged by size: 2120 1148 700 677 389 245 211 7 NaeI GCC'GGC Cuts at: 2500 2793 2500 Size: 293 5204 NarI GG'CG_CC Cuts at: 2647 2647 Size: 5497 NcoI C'CATG_G Cuts at: 513 1065 2004 2676 513 Size: 552 939 672 3334 Fragments arranged by size: 3334 939 672 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NgoMIV G'CCGG_C Cuts at: 2498 2791 2498 Size: 293 5204 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PstI C_TGCA'G Cuts at: 830 2849 830 Size: 2019 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2845 3980 4661 4852 826 Size: 219 1800 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2100 2100 Size: 5497 SfoI GGC'GCC Cuts at: 2648 2648 Size: 5497 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2047 2090 2824 1872 Size: 175 43 734 4545 Fragments arranged by size: 4545 734 175 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 3601 4502 4764 820 Size: 197 926 198 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 198 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 2147 1517 Size: 630 4867 StyI C'CwwG_G Cuts at: 513 1065 2004 2676 2936 513 Size: 552 939 672 260 3074 Fragments arranged by size: 3074 939 672 552 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3524 3541 3694 3879 4324 325 Size: 1255 1944 17 153 185 445 1498 Fragments arranged by size: 1944 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2045 2088 2822 1870 Size: 175 43 734 4545 Fragments arranged by size: 4545 734 175 43 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI