(Circular) (Six-base) MAPSORT of: pFN21AB9614.seq Check: 3466 from: 1 to: 6211 pFN21AB9614 6211 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2070 2689 2853 3109 4274 5658 5926 1632 Size: 438 619 164 256 1165 1384 268 1917 Fragments arranged by size: 1917 1384 1165 619 438 268 256 164 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 2139 2429 5110 6087 1203 Size: 936 290 2681 977 1327 Fragments arranged by size: 2681 1327 977 936 290 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 3462 4940 3462 Size: 1478 4733 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6211 AlwNI CAG_nnn'CTG Cuts at: 3954 5526 3954 Size: 1572 4639 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 2043 2084 2571 2655 2713 2823 2907 2991 Size: 550 41 487 84 58 110 84 84 Cuts at: 2991 3075 3159 3243 3327 3411 3520 3746 3898 Size: 84 84 84 84 84 109 226 152 Cuts at: 3898 6007 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 550 487 226 152 110 109 84 84 84 84 84 84 84 84 58 41 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3536 1870 Size: 73 1593 4545 Fragments arranged by size: 4545 1593 73 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2562 3532 4987 618 Size: 325 217 627 775 970 1455 1842 Fragments arranged by size: 1842 1455 970 775 627 325 217 BanII G_rGCy'C Cuts at: 1391 3473 3530 1391 Size: 2082 57 4072 Fragments arranged by size: 4072 2082 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 2789 3461 928 Size: 971 243 647 672 3678 Fragments arranged by size: 3678 971 672 647 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4402 4436 1903 Size: 34 2465 34 3678 Fragments arranged by size: 3678 2465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4822 136 Size: 107 122 71 631 605 3150 1525 Fragments arranged by size: 3150 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 3628 3628 Size: 6211 Bme1580I G_kGCm'C Cuts at: 1790 4272 5428 1790 Size: 2482 1156 2573 Fragments arranged by size: 2573 2482 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3942 4900 442 Size: 416 53 83 399 24 2525 958 1753 Fragments arranged by size: 2525 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 2685 2769 2937 3189 3273 3441 4871 Size: 1362 84 84 168 252 84 168 1430 Cuts at: 4871 1239 Size: 2579 Fragments arranged by size: 2579 1430 1362 252 168 168 84 84 84 Bpu10I CC'TnA_GC Cuts at: 2884 2884 Size: 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2230 4336 5201 5499 2230 Size: 2106 865 298 2942 Fragments arranged by size: 2942 2106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2665 4874 882 Size: 617 1166 2209 2219 Fragments arranged by size: 2219 2209 1166 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4018 4400 5035 275 Size: 53 83 186 936 2485 382 635 1451 Fragments arranged by size: 2485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5986 Fragments arranged by size: 5986 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2184 3470 1186 Size: 998 1286 3927 Fragments arranged by size: 3927 1286 998 BseYI C'CCAG_C Cuts at: 2294 5414 5910 2294 Size: 3120 496 2595 Fragments arranged by size: 3120 2595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5638 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3473 4272 4357 5428 3473 Size: 799 85 1071 4256 Fragments arranged by size: 4256 1071 799 85 BsmI GAATG_Cn' Cuts at: 2855 3191 3385 3722 3815 2855 Size: 336 194 337 93 5251 Fragments arranged by size: 5251 337 336 194 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3473 3530 4272 4357 5428 1391 Size: 399 1683 57 742 85 1071 2174 Fragments arranged by size: 2174 1683 1071 742 399 85 57 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 2110 4100 5830 2110 Size: 1990 1730 2491 Fragments arranged by size: 2491 1990 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3566 844 Size: 2722 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 4706 4880 65 Size: 1183 1612 1846 174 1396 Fragments arranged by size: 1846 1612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstYI r'GATC_y Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206 Size: 1569 367 385 17 1452 86 12 346 Cuts at: 6206 1972 Size: 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2196 2196 Size: 6211 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 4558 Fragments arranged by size: 4558 939 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 3727 4521 4541 3727 Size: 794 20 5397 Fragments arranged by size: 5397 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2842 2877 529 Size: 35 2278 35 3863 Fragments arranged by size: 3863 2278 35 35 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 3043 3658 Size: 54 1425 50 275 18 529 684 615 Cuts at: 3658 4547 6176 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 684 615 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 4141 1353 Size: 813 1975 3423 Fragments arranged by size: 3423 1975 813 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3229 4798 5172 5318 387 Size: 1064 138 1640 1569 374 146 1280 Fragments arranged by size: 1640 1569 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3471 3471 Size: 6211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2601 2685 2689 2769 2853 2937 Size: 393 438 531 84 4 80 84 84 Cuts at: 2937 3109 3189 3273 3441 4274 4871 5658 5926 Size: 172 80 84 168 833 597 787 268 Cuts at: 5926 1239 Size: 1524 Fragments arranged by size: 1524 833 787 597 531 438 393 268 172 168 84 84 84 84 80 80 4 EcoRI G'AATT_C Cuts at: 2571 2655 2823 2991 3075 3159 3243 3411 3520 Size: 84 168 168 84 84 84 168 109 Cuts at: 3520 3898 2571 Size: 378 4884 Fragments arranged by size: 4884 378 168 168 168 109 84 84 84 84 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 5358 1678 Size: 3680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2179 2198 2206 2211 Size: 32 135 32 602 5 19 8 5 Cuts at: 2211 2230 3448 3480 4852 4884 4926 4958 1373 Size: 19 1218 32 1372 32 42 32 2626 Fragments arranged by size: 2626 1372 1218 602 135 42 32 32 32 32 32 19 19 8 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3555 3801 669 Size: 988 281 1617 246 3079 Fragments arranged by size: 3079 1617 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 3446 3508 5325 5509 1305 Size: 819 1322 62 1817 184 2007 Fragments arranged by size: 2007 1817 1322 819 184 62 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 3045 10 Size: 54 1425 343 529 684 3176 Fragments arranged by size: 3176 1425 684 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2764 2932 3084 3492 4169 4528 4687 Size: 648 1598 168 152 408 677 359 159 Cuts at: 4687 518 Size: 2042 Fragments arranged by size: 2042 1598 677 648 408 359 168 159 152 MspA1I CmG'CkG Cuts at: 1606 3627 4304 5452 5697 1606 Size: 2021 677 1148 245 2120 Fragments arranged by size: 2120 2021 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 4558 Fragments arranged by size: 4558 939 552 162 NdeI CA'TA_TG Cuts at: 387 2481 2761 387 Size: 2094 280 3837 Fragments arranged by size: 3837 2094 280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 2143 2433 3569 5114 1132 Size: 97 914 290 1136 1545 2229 Fragments arranged by size: 2229 1545 1136 914 290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6211 PciI A'CATG_T Cuts at: 2139 2429 5110 2139 Size: 290 2681 3240 Fragments arranged by size: 3240 2681 290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6211 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PstI C_TGCA'G Cuts at: 830 2291 3563 830 Size: 1461 1272 3478 Fragments arranged by size: 3478 1461 1272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 SacI G_AGCT'C Cuts at: 3473 3473 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2287 3559 4694 5375 5566 826 Size: 219 1242 1272 1135 681 191 1471 Fragments arranged by size: 1471 1272 1242 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2245 4315 5216 5478 820 Size: 197 926 302 2070 901 262 1553 Fragments arranged by size: 2070 1553 926 901 302 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 2618 2889 3122 1517 Size: 1101 271 233 4606 Fragments arranged by size: 4606 1101 271 233 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2104 2166 3650 513 Size: 552 939 30 70 62 1484 3074 Fragments arranged by size: 3074 1484 939 552 70 62 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4238 4255 4408 4593 5038 325 Size: 1255 2658 17 153 185 445 1498 Fragments arranged by size: 2658 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4457 6169 96 Size: 274 80 33 51 1443 2480 1712 138 Fragments arranged by size: 2480 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 6171 6203 2457 Size: 32 3682 32 2465 Fragments arranged by size: 3682 2465 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 2302 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 2085 2545 2954 3122 4340 2085 Size: 460 409 168 1218 3956 Fragments arranged by size: 3956 1218 460 409 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI