(Circular) (Six-base) MAPSORT of: pFN21AE9553.seq Check: 8177 from: 1 to: 6823 pFN21AE9553 6823 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3903 4166 1937 Size: 1966 263 4594 Fragments arranged by size: 4594 1966 263 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3247 3677 4011 4886 6270 6538 1632 Size: 1615 430 334 875 1384 268 1917 Fragments arranged by size: 1917 1615 1384 875 430 334 268 AflII C'TTAA_G Cuts at: 820 1017 2498 820 Size: 197 1481 5145 Fragments arranged by size: 5145 1481 197 AflIII A'CryG_T Cuts at: 1203 2030 2565 5722 6699 1203 Size: 827 535 3157 977 1327 Fragments arranged by size: 3157 1327 977 827 535 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 5552 5552 Size: 6823 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4025 1184 Size: 2841 3982 AlwNI CAG_nnn'CTG Cuts at: 4121 4566 6138 4121 Size: 445 1572 4806 Fragments arranged by size: 4806 1572 445 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2268 2429 3239 4132 4358 4510 6619 1493 Size: 775 161 810 893 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 810 775 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 2330 3455 5377 160 Size: 872 1298 1125 1922 1606 Fragments arranged by size: 1922 1606 1298 1125 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4148 1870 Size: 73 2205 4545 Fragments arranged by size: 4545 2205 73 AvrII C'CTAG_G Cuts at: 2827 3745 2827 Size: 918 5905 BamHI G'GATC_C Cuts at: 2346 4153 4520 2346 Size: 1807 367 4649 Fragments arranged by size: 4649 1807 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4144 5599 618 Size: 325 217 627 2357 1455 1842 Fragments arranged by size: 2357 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3153 4142 1391 Size: 1762 989 4072 Fragments arranged by size: 4072 1762 989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3253 3469 928 Size: 971 1354 216 4282 Fragments arranged by size: 4282 1354 971 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 3358 1548 Size: 1810 5013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3921 5434 136 Size: 107 122 71 631 605 2249 1513 1525 Fragments arranged by size: 2249 1525 1513 631 605 122 107 71 BglII A'GATC_T Cuts at: 6818 6818 Size: 6823 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 2061 4884 6040 1790 Size: 271 2823 1156 2573 Fragments arranged by size: 2823 2573 1156 271 BmgBI CAC'GTC Cuts at: 1318 2031 1318 Size: 713 6110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4014 4554 5512 Size: 416 53 83 399 24 2597 540 958 Cuts at: 5512 442 Size: 1753 Fragments arranged by size: 2597 1753 958 540 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3822 1056 Size: 2766 4057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3865 5483 1239 Size: 2626 1618 2579 Fragments arranged by size: 2626 2579 1618 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4948 5813 6111 4948 Size: 865 298 5660 Fragments arranged by size: 5660 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2692 3358 5486 882 Size: 617 1193 666 2128 2219 Fragments arranged by size: 2219 2128 1193 666 617 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5256 5654 5928 6075 6486 5256 Size: 398 274 147 411 5593 Fragments arranged by size: 5593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6598 Fragments arranged by size: 6598 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 1186 Size: 1189 5634 BseYI C'CCAG_C Cuts at: 3515 6026 6522 3515 Size: 2511 496 3816 Fragments arranged by size: 3816 2511 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 5034 6062 1540 Size: 275 186 2272 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4884 4969 6040 4884 Size: 85 1071 5667 Fragments arranged by size: 5667 1071 85 BsmI GAATG_Cn' Cuts at: 4334 4427 4334 Size: 93 6730 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 3153 4142 4884 4969 6040 1391 Size: 399 271 1092 989 742 85 1071 2174 Fragments arranged by size: 2174 1092 1071 989 742 399 271 85 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2264 3865 4003 4178 844 Size: 1420 1601 138 175 3489 Fragments arranged by size: 3489 1601 1420 175 138 BsrBI CCG'CTC Cuts at: 1394 1823 4710 1394 Size: 429 2887 3507 Fragments arranged by size: 3507 2887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5318 5492 65 Size: 1183 4070 174 1396 Fragments arranged by size: 4070 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5467 5654 1700 Size: 110 3657 187 2869 Fragments arranged by size: 3657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 2781 794 Size: 1987 4836 BstBI TT'CG_AA Cuts at: 3250 5627 3250 Size: 2377 4446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 3676 3676 Size: 6823 BstYI r'GATC_y Cuts at: 1972 2346 3544 4153 4520 4905 4922 6374 6460 Size: 374 1198 609 367 385 17 1452 86 Cuts at: 6460 6472 6818 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1198 609 385 374 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 6823 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 3675 513 Size: 552 939 162 1509 3661 Fragments arranged by size: 3661 1509 939 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6084 Fragments arranged by size: 6084 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3931 4339 5133 5153 2982 Size: 949 408 794 20 4652 Fragments arranged by size: 4652 949 794 408 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3046 3081 529 Size: 35 2482 35 4271 Fragments arranged by size: 4271 2482 35 35 DraI TTT'AAA Cuts at: 2575 3526 4127 2575 Size: 951 601 5271 Fragments arranged by size: 5271 951 601 DraIII CAC_nnn'GTG Cuts at: 2898 2898 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2822 5830 809 Size: 507 1506 3008 1802 Fragments arranged by size: 3008 1802 1506 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3214 4270 5159 Size: 54 1425 50 275 18 1384 1056 889 Cuts at: 5159 6788 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1384 1056 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 1537 Size: 275 2458 4090 Fragments arranged by size: 4090 2458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3162 3559 4753 1353 Size: 719 1090 397 1194 3423 Fragments arranged by size: 3423 1194 1090 719 397 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5410 5784 5930 387 Size: 1064 138 3821 374 146 1280 Fragments arranged by size: 3821 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3247 3677 3865 4011 4886 5483 6270 Size: 393 1615 430 188 146 875 597 787 Cuts at: 6270 6538 1239 Size: 268 1524 Fragments arranged by size: 1615 1524 875 787 597 430 393 268 188 146 EcoO109I rG'GnC_Cy Cuts at: 4015 4015 Size: 6823 EcoRI G'AATT_C Cuts at: 4132 4510 4132 Size: 378 6445 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 5970 1678 Size: 4292 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3294 3326 3534 3566 5464 Size: 32 135 32 1722 32 208 32 1898 Cuts at: 5464 5496 5538 5570 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1898 1722 208 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 3904 4167 4413 669 Size: 988 281 90 1876 263 246 3079 Fragments arranged by size: 3079 1876 988 281 263 246 90 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3466 5937 6121 1305 Size: 2161 2471 184 2007 Fragments arranged by size: 2471 2161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5001 Fragments arranged by size: 5001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4781 5140 5299 518 Size: 648 843 2772 359 159 2042 Fragments arranged by size: 2772 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2168 3267 4239 4916 6064 6309 1606 Size: 562 1099 972 677 1148 245 2120 Fragments arranged by size: 2120 1148 1099 972 677 562 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3675 513 Size: 552 939 1671 3661 Fragments arranged by size: 3661 1671 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NheI G'CTAG_C Cuts at: 1052 3818 1052 Size: 2766 4057 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2569 3504 4181 5726 1132 Size: 97 1340 935 677 1545 2229 Fragments arranged by size: 2229 1545 1340 935 677 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 2565 5722 2565 Size: 3157 3666 PflMI CCAn_nnn'nTGG Cuts at: 1108 2922 1108 Size: 1814 5009 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4015 4015 Size: 6823 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PstI C_TGCA'G Cuts at: 830 2938 3721 4175 830 Size: 2108 783 454 3478 Fragments arranged by size: 3478 2108 783 454 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 SacII CC_GC'GG Cuts at: 2169 2169 Size: 6823 SalI G'TCGA_C Cuts at: 1936 3902 4165 1936 Size: 1966 263 4594 Fragments arranged by size: 4594 1966 263 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 3570 5071 6783 3570 Size: 1501 1712 3610 Fragments arranged by size: 3610 1712 1501 SexAI A'CCwGG_T Cuts at: 3007 3007 Size: 6823 SfcI C'TryA_G Cuts at: 826 1045 2934 3572 3696 3717 4171 5306 5987 Size: 219 1889 638 124 21 454 1135 681 Cuts at: 5987 6178 826 Size: 191 1471 Fragments arranged by size: 1889 1471 1135 681 638 454 219 191 124 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 4927 5828 6090 820 Size: 197 926 555 2429 901 262 1553 Fragments arranged by size: 2429 1553 926 901 555 262 197 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 2625 4747 5 Size: 47 2573 2122 2081 Fragments arranged by size: 2573 2122 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6823 StyI C'CwwG_G Cuts at: 513 1065 2004 2489 2827 3675 3745 4262 513 Size: 552 939 485 338 848 70 517 3074 Fragments arranged by size: 3074 939 848 552 517 485 338 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 4850 4867 5020 5205 5650 325 Size: 1255 1313 1957 17 153 185 445 1498 Fragments arranged by size: 1957 1498 1313 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2745 3115 3568 Size: 274 80 33 51 1443 768 370 453 Cuts at: 3568 5069 6781 96 Size: 1501 1712 138 Fragments arranged by size: 1712 1501 1443 768 453 370 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2997 3029 6783 6815 2997 Size: 32 3754 32 3005 Fragments arranged by size: 3754 3005 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2471 2471 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 2268 2617 4952 2268 Size: 349 2335 4139 Fragments arranged by size: 4139 2335 349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI