(Circular) (Six-base) MAPSORT of: pFN21AB9608.seq Check: 7152 from: 1 to: 5557 pFN21AB9608 5557 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2049 2049 Size: 5557 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2518 2602 2686 3620 5004 5272 1632 Size: 886 84 84 934 1384 268 1917 Fragments arranged by size: 1917 1384 934 886 268 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 4456 5433 1203 Size: 3253 977 1327 Fragments arranged by size: 3253 1327 977 AgeI A'CCGG_T Cuts at: 2760 4388 2760 Size: 1628 3929 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 2325 2661 1166 Size: 1159 336 4062 Fragments arranged by size: 4062 1159 336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 3300 4872 3300 Size: 1572 3985 ApaLI G'TGCA_C Cuts at: 2680 2764 3614 4770 2680 Size: 84 850 1156 3467 Fragments arranged by size: 3467 1156 850 84 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2368 2882 1870 Size: 73 256 169 514 4545 Fragments arranged by size: 4545 514 256 169 73 AvrII C'CTAG_G Cuts at: 2491 2491 Size: 5557 BamHI G'GATC_C Cuts at: 2100 2316 2400 2887 3254 2100 Size: 216 84 487 367 4403 Fragments arranged by size: 4403 487 367 216 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2878 4333 618 Size: 325 217 627 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 627 325 217 BanII G_rGCy'C Cuts at: 1391 2200 2798 2876 1391 Size: 809 598 78 4072 Fragments arranged by size: 4072 809 598 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4168 136 Size: 107 122 71 631 605 2496 1525 Fragments arranged by size: 2496 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2266 2684 2768 3618 4774 1790 Size: 476 418 84 850 1156 2573 Fragments arranged by size: 2573 1156 850 476 418 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 3288 4246 Size: 416 53 83 399 24 843 1028 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1028 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2555 1056 Size: 1499 4058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2597 2614 4217 1239 Size: 1358 17 1603 2579 Fragments arranged by size: 2579 1603 1358 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2662 4220 882 Size: 617 1163 1558 2219 Fragments arranged by size: 2219 1558 1163 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2565 2760 3990 4388 4662 4809 5220 2565 Size: 195 1230 398 274 147 411 2902 Fragments arranged by size: 2902 1230 411 398 274 195 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5332 Fragments arranged by size: 5332 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5557 BseYI C'CCAG_C Cuts at: 2840 4760 5256 2840 Size: 1920 496 3141 Fragments arranged by size: 3141 1920 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4984 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3768 4796 1540 Size: 275 186 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2684 2728 2768 2798 3618 3703 4774 2684 Size: 44 40 30 820 85 1071 3467 Fragments arranged by size: 3467 1071 820 85 44 40 30 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2266 2684 2728 2768 2798 2876 Size: 399 410 66 418 44 40 30 78 Cuts at: 2876 3618 3703 4774 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 418 410 399 85 78 66 44 40 30 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2912 844 Size: 1205 863 3489 Fragments arranged by size: 3489 1205 863 BsrBI CCG'CTC Cuts at: 1394 1823 2398 3444 1394 Size: 429 575 1046 3507 Fragments arranged by size: 3507 1046 575 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2217 4052 4226 65 Size: 1183 969 1835 174 1396 Fragments arranged by size: 1835 1396 1183 969 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 4201 4388 1700 Size: 110 950 1441 187 2869 Fragments arranged by size: 2869 1441 950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 2393 3617 4629 2393 Size: 1224 1012 3321 Fragments arranged by size: 3321 1224 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2100 2316 2400 2887 3254 3639 3656 5108 Size: 128 216 84 487 367 385 17 1452 Cuts at: 5108 5194 5206 5552 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 487 385 367 346 216 128 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2046 2046 Size: 5557 BtgI C'CryG_G Cuts at: 513 1065 2004 2174 513 Size: 552 939 170 3896 Fragments arranged by size: 3896 939 552 170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 2017 3073 3867 3887 2017 Size: 1056 794 20 3687 Fragments arranged by size: 3687 1056 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DraIII CAC_nnn'GTG Cuts at: 2688 2772 2688 Size: 84 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2501 3004 3893 Size: 54 1425 50 275 18 671 503 889 Cuts at: 3893 5522 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 671 503 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2606 3487 1353 Size: 1053 200 881 3423 Fragments arranged by size: 3423 1053 881 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2710 2798 4144 4518 4664 387 Size: 1064 138 1121 88 1346 374 146 1280 Fragments arranged by size: 1346 1280 1121 1064 374 146 138 88 EcoICRI GAG'CTC Cuts at: 2796 2796 Size: 5557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2518 2597 2602 2614 2686 3620 4217 Size: 393 886 79 5 12 72 934 597 Cuts at: 4217 5004 5272 1239 Size: 787 268 1524 Fragments arranged by size: 1524 934 886 787 597 393 268 79 72 12 5 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2049 2045 Size: 4 5553 EcoO109I rG'GnC_Cy Cuts at: 2212 2212 Size: 5557 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 EcoRV GAT'ATC Cuts at: 2710 2710 Size: 5557 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 2461 4704 1678 Size: 783 2243 2531 Fragments arranged by size: 2531 2243 783 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2123 2155 2785 2817 4198 Size: 32 135 32 551 32 630 32 1381 Cuts at: 4198 4230 4272 4304 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1381 630 551 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2319 2403 2739 4671 4855 1305 Size: 816 198 84 336 1932 184 2007 Fragments arranged by size: 2007 1932 816 336 198 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3735 Fragments arranged by size: 3735 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2325 2661 3515 3874 4033 518 Size: 648 1159 336 854 359 159 2042 Fragments arranged by size: 2042 1159 854 648 359 336 159 MspA1I CmG'CkG Cuts at: 1606 2314 2511 2734 2973 3650 4798 5043 1606 Size: 708 197 223 239 677 1148 245 2120 Fragments arranged by size: 2120 1148 708 677 245 239 223 197 NcoI C'CATG_G Cuts at: 513 1065 2004 2174 513 Size: 552 939 170 3896 Fragments arranged by size: 3896 939 552 170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 2551 1052 Size: 1499 4058 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PfoI T'CCnGG_A Cuts at: 2789 2789 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2212 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2909 830 Size: 2079 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SacI G_AGCT'C Cuts at: 2798 2798 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SanDI GG'GwC_CC Cuts at: 2212 2212 Size: 5557 SapI GCTCTTCn'nnn_ Cuts at: 2606 2606 Size: 5557 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2905 4040 4721 4912 826 Size: 219 1860 1135 681 191 1471 Fragments arranged by size: 1860 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2199 2368 3661 4562 4824 820 Size: 197 926 256 169 1293 901 262 1553 Fragments arranged by size: 1553 1293 926 901 262 256 197 169 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2069 2174 2228 2491 2996 513 Size: 552 939 65 105 54 263 505 3074 Fragments arranged by size: 3074 939 552 505 263 105 65 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 3584 3601 3754 3939 4384 325 Size: 1255 1089 915 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2172 2172 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 2199 2368 1943 Size: 256 169 5132 Fragments arranged by size: 5132 256 169 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 2615 2783 3686 2615 Size: 168 903 4486 Fragments arranged by size: 4486 903 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI