(Circular) (Six-base) MAPSORT of: pFN21AB9599.seq Check: 3585 from: 1 to: 6022 pFN21AB9599 6022 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2161 2643 2161 Size: 482 5540 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 4085 5469 5737 1632 Size: 681 1772 1384 268 1917 Fragments arranged by size: 1917 1772 1384 681 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 2562 4921 5898 1203 Size: 1359 2359 977 1327 Fragments arranged by size: 2359 1359 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 3013 3765 5337 3013 Size: 752 1572 3698 Fragments arranged by size: 3698 1572 752 ApaI G_GGCC'C Cuts at: 2710 3318 2710 Size: 608 5414 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 3331 3557 3709 5818 1493 Size: 1838 226 152 2109 1697 Fragments arranged by size: 2109 1838 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2202 2230 2623 2731 2756 3347 1870 Size: 73 259 28 393 108 25 591 4545 Fragments arranged by size: 4545 591 393 259 108 73 28 25 BamHI G'GATC_C Cuts at: 3352 3719 3352 Size: 367 5655 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2266 2833 2872 3165 Size: 325 217 627 465 14 567 39 293 Cuts at: 3165 3343 4798 618 Size: 178 1455 1842 Fragments arranged by size: 1842 1455 627 567 465 325 293 217 178 39 14 BanII G_rGCy'C Cuts at: 1391 2632 2710 3218 3318 3341 1391 Size: 1241 78 508 100 23 4072 Fragments arranged by size: 4072 1241 508 100 78 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 2118 928 Size: 971 180 39 4832 Fragments arranged by size: 4832 971 180 39 BbvCI CC'TCA_GC Cuts at: 2906 2906 Size: 6022 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2777 2811 4213 4247 1903 Size: 34 840 34 1402 34 3678 Fragments arranged by size: 3678 1402 840 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BfrBI ATG'CAT Cuts at: 2957 2957 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2520 3182 4633 Size: 107 122 71 631 605 848 662 1451 Cuts at: 4633 136 Size: 1525 Fragments arranged by size: 1525 1451 848 662 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 2022 2384 3439 2022 Size: 362 1055 4605 Fragments arranged by size: 4605 1055 362 Bme1580I G_kGCm'C Cuts at: 1790 2269 2710 2836 3168 3318 4083 5239 1790 Size: 479 441 126 332 150 765 1156 2573 Fragments arranged by size: 2573 1156 765 479 441 332 150 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3277 3277 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2557 3043 4682 1239 Size: 902 416 486 1639 2579 Fragments arranged by size: 2579 1639 902 486 416 Bpu10I CC'TnA_GC Cuts at: 2906 2906 Size: 6022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4147 5012 5310 4147 Size: 865 298 4859 Fragments arranged by size: 4859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4685 882 Size: 617 3186 2219 Fragments arranged by size: 3186 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2764 2935 3829 4211 Size: 53 83 186 936 1231 171 894 382 Cuts at: 4211 4846 275 Size: 635 1451 Fragments arranged by size: 1451 1231 936 894 635 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4633 Fragments arranged by size: 4633 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2132 2135 2314 2784 2932 3128 1186 Size: 928 18 3 179 470 148 196 4080 Fragments arranged by size: 4080 928 470 196 179 148 18 3 BseYI C'CCAG_C Cuts at: 3157 5225 5721 3157 Size: 2068 496 3458 Fragments arranged by size: 3458 2068 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2646 2660 2765 1163 Size: 573 910 14 105 4420 Fragments arranged by size: 4420 910 573 105 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3035 3067 3177 3472 4233 5261 Size: 275 186 1034 32 110 295 761 1028 Cuts at: 5261 1540 Size: 2301 Fragments arranged by size: 2301 1034 1028 761 295 275 186 110 32 BsiHKAI G_wGCw'C Cuts at: 2211 4083 4168 5239 2211 Size: 1872 85 1071 2994 Fragments arranged by size: 2994 1872 1071 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2755 2755 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2269 2632 2710 2836 3168 3218 Size: 399 421 58 363 78 126 332 50 Cuts at: 3218 3318 3341 4083 4168 5239 1391 Size: 100 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 363 332 126 100 85 78 58 50 23 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2643 2665 3377 844 Size: 1317 482 22 712 3489 Fragments arranged by size: 3489 1317 712 482 22 BsrBI CCG'CTC Cuts at: 1394 1823 3001 3909 1394 Size: 429 1178 908 3507 Fragments arranged by size: 3507 1178 908 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2308 4666 4853 1700 Size: 110 498 2358 187 2869 Fragments arranged by size: 2869 2358 498 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstYI r'GATC_y Cuts at: 1972 2496 3352 3719 4104 4121 5573 5659 5671 Size: 524 856 367 385 17 1452 86 12 Cuts at: 5671 6017 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 856 524 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2346 513 Size: 552 1281 4189 Fragments arranged by size: 4189 1281 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2317 3213 487 Size: 683 56 1091 896 3296 Fragments arranged by size: 3296 1091 896 683 56 BtsI GCAGTG_nn' Cuts at: 3538 4332 4352 3538 Size: 794 20 5208 Fragments arranged by size: 5208 794 20 ClaI AT'CG_AT Cuts at: 1554 2085 1554 Size: 531 5491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2489 5029 809 Size: 507 1173 2540 1802 Fragments arranged by size: 2540 1802 1173 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2410 3174 Size: 54 1425 50 275 18 519 61 764 Cuts at: 3174 3234 3300 3469 4358 5987 8 Size: 60 66 169 889 1629 43 Fragments arranged by size: 1629 1425 889 764 519 275 169 66 61 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3174 3469 1537 Size: 275 1362 295 4090 Fragments arranged by size: 4090 1362 295 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3952 1353 Size: 2599 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2450 2966 3264 4609 4983 5129 Size: 1064 138 861 516 298 1345 374 146 Cuts at: 5129 387 Size: 1280 Fragments arranged by size: 1345 1280 1064 861 516 374 298 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2313 2557 3043 4085 4682 5469 Size: 393 509 172 244 486 1042 597 787 Cuts at: 5469 5737 1239 Size: 268 1524 Fragments arranged by size: 1524 1042 787 597 509 486 393 268 244 172 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 6022 EcoO109I rG'GnC_Cy Cuts at: 2706 2707 2736 3258 3314 3315 2706 Size: 1 29 522 56 1 5413 Fragments arranged by size: 5413 522 56 29 1 1 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FspI TGC'GCA Cuts at: 3017 4528 3017 Size: 1511 4511 HaeII r_GCGC'y Cuts at: 1678 2029 2377 2611 3188 5169 1678 Size: 351 348 234 577 1981 2531 Fragments arranged by size: 2531 1981 577 351 348 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2196 2228 2537 2569 4663 Size: 32 135 32 624 32 309 32 2094 Cuts at: 4663 4695 4737 4769 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2094 624 309 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3059 5136 5320 1305 Size: 1754 2077 184 2007 Fragments arranged by size: 2077 2007 1754 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 3681 Fragments arranged by size: 3681 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3980 4339 4498 518 Size: 648 1179 1635 359 159 2042 Fragments arranged by size: 2042 1635 1179 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 3103 3438 4115 5263 5508 1606 Size: 782 715 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 715 677 335 245 NaeI GCC'GGC Cuts at: 2310 2310 Size: 6022 NcoI C'CATG_G Cuts at: 513 1065 2346 513 Size: 552 1281 4189 Fragments arranged by size: 4189 1281 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NgoMIV G'CCGG_C Cuts at: 2308 2308 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NsiI A_TGCA'T Cuts at: 2959 2959 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2566 3380 4925 1132 Size: 97 1337 814 1545 2229 Fragments arranged by size: 2229 1545 1337 814 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 2562 4921 2562 Size: 2359 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6022 PfoI T'CCnGG_A Cuts at: 2055 2055 Size: 6022 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3258 3258 Size: 6022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2706 3314 2706 Size: 608 5414 PstI C_TGCA'G Cuts at: 830 2172 2891 3374 830 Size: 1342 719 483 3478 Fragments arranged by size: 3478 1342 719 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2168 2887 3370 4505 5186 5377 826 Size: 219 1123 719 483 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 719 681 483 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2625 2733 2758 3349 1872 Size: 753 108 25 591 4545 Fragments arranged by size: 4545 753 591 108 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 4126 5027 5289 820 Size: 197 926 2183 901 262 1553 Fragments arranged by size: 2183 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2273 2346 2911 3144 3461 513 Size: 552 1208 73 565 233 317 3074 Fragments arranged by size: 3074 1208 565 552 317 233 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4268 5980 96 Size: 274 80 33 51 1443 2291 1712 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5982 6014 5982 Size: 32 5990 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2623 2731 2756 3347 1870 Size: 753 108 25 591 4545 Fragments arranged by size: 4545 753 591 108 25 XmnI GAAnn'nnTTC Cuts at: 2600 4151 2600 Size: 1551 4471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI