(Circular) (Six-base) MAPSORT of: pFN21AB9598.seq Check: 9009 from: 1 to: 6139 pFN21AB9598 6139 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2745 2745 Size: 6139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4202 5586 5854 1632 Size: 2570 1384 268 1917 Fragments arranged by size: 2570 1917 1384 268 AfeI AGC'GCT Cuts at: 3285 3285 Size: 6139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2037 2673 5038 6015 1203 Size: 834 636 2365 977 1327 Fragments arranged by size: 2365 1327 977 834 636 AgeI A'CCGG_T Cuts at: 2938 4970 2938 Size: 2032 4107 AhdI GACnn_n'nnGTC Cuts at: 2662 4868 2662 Size: 2206 3933 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2711 1184 Size: 1527 4612 AlwNI CAG_nnn'CTG Cuts at: 2239 3373 3882 5454 2239 Size: 1134 509 1572 2924 Fragments arranged by size: 2924 1572 1134 509 ApaI G_GGCC'C Cuts at: 3167 3167 Size: 6139 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AscI GG'CGCG_CC Cuts at: 2295 2295 Size: 6139 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2398 2629 2971 3019 3263 3464 1870 Size: 73 455 231 342 48 244 201 4545 Fragments arranged by size: 4545 455 342 244 231 201 73 48 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2197 2700 2801 2855 Size: 325 217 627 398 12 503 101 54 Cuts at: 2855 2935 3050 3080 3089 3460 4915 618 Size: 80 115 30 9 371 1455 1842 Fragments arranged by size: 1842 1455 627 503 398 371 325 217 115 101 80 54 30 12 9 BanII G_rGCy'C Cuts at: 1391 2477 3167 3185 3458 1391 Size: 1086 690 18 273 4072 Fragments arranged by size: 4072 1086 690 273 18 BbeI G_GCGC'C Cuts at: 2704 2805 3054 3084 2704 Size: 101 249 30 5759 Fragments arranged by size: 5759 249 101 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5168 BbvCI CC'TCA_GC Cuts at: 2217 2217 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2829 3059 4750 Size: 107 122 71 631 605 1157 230 1691 Cuts at: 4750 136 Size: 1525 Fragments arranged by size: 1691 1525 1157 631 605 230 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3314 3556 3314 Size: 242 5897 Bme1580I G_kGCm'C Cuts at: 1790 2190 3074 3092 3167 4200 5356 1790 Size: 400 884 18 75 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 884 400 75 18 BmgBI CAC'GTC Cuts at: 1318 2071 2290 1318 Size: 753 219 5167 Fragments arranged by size: 5167 753 219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 3870 4828 Size: 416 53 83 399 24 1808 645 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1808 1753 958 645 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2891 4799 1239 Size: 1652 1908 2579 Fragments arranged by size: 2579 1908 1652 Bpu10I CC'TnA_GC Cuts at: 2217 2495 2217 Size: 278 5861 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3402 4264 5129 5427 3402 Size: 862 865 298 4114 Fragments arranged by size: 4114 865 862 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2701 2802 3051 3081 Size: 53 83 186 936 1168 101 249 30 Cuts at: 3081 3946 4328 4963 275 Size: 865 382 635 1451 Fragments arranged by size: 1451 1168 936 865 635 382 249 186 101 83 53 30 BsaWI w'CCGG_w Cuts at: 2938 3362 4572 4970 5244 5391 5802 2938 Size: 424 1210 398 274 147 411 3275 Fragments arranged by size: 3275 1210 424 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 1186 Size: 1165 4974 BseYI C'CCAG_C Cuts at: 2980 3197 5342 5838 2980 Size: 217 2145 496 3281 Fragments arranged by size: 3281 2145 496 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 3293 1163 Size: 573 1083 474 4009 Fragments arranged by size: 4009 1083 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 2262 2799 2821 2853 2886 Size: 275 186 63 198 537 22 32 33 Cuts at: 2886 2997 3063 3131 3393 3411 3589 4350 5378 Size: 111 66 68 262 18 178 761 1028 Cuts at: 5378 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 537 275 262 198 186 178 111 68 66 63 33 32 22 18 BsiHKAI G_wGCw'C Cuts at: 2736 4200 4285 5356 2736 Size: 1464 85 1071 3519 Fragments arranged by size: 3519 1464 1071 85 BsiWI C'GTAC_G Cuts at: 2916 2916 Size: 6139 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2477 2736 3074 3092 3167 3185 Size: 399 400 287 259 338 18 75 18 Cuts at: 3185 3458 4200 4285 5356 1391 Size: 273 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 338 287 273 259 85 75 18 18 BspEI T'CCGG_A Cuts at: 3362 5802 3362 Size: 2440 3699 BspHI T'CATG_A Cuts at: 2757 4028 5758 2757 Size: 1271 1730 3138 Fragments arranged by size: 3138 1730 1271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2120 2745 3494 844 Size: 1276 625 749 3489 Fragments arranged by size: 3489 1276 749 625 BsrBI CCG'CTC Cuts at: 1394 1823 2352 2499 3416 4026 1394 Size: 429 529 147 917 610 3507 Fragments arranged by size: 3507 917 610 529 429 147 BsrDI GCAATG_nn' Cuts at: 65 1248 2243 4634 4808 65 Size: 1183 995 2391 174 1396 Fragments arranged by size: 2391 1396 1183 995 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2798 2852 2929 2938 3206 3430 4783 Size: 110 988 54 77 9 268 224 1353 Cuts at: 4783 4970 1700 Size: 187 2869 Fragments arranged by size: 2869 1353 988 268 224 187 110 77 54 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 2295 3123 1925 Size: 370 828 4941 Fragments arranged by size: 4941 828 370 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstYI r'GATC_y Cuts at: 1972 2607 3469 3836 4221 4238 5690 5776 5788 Size: 635 862 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 862 635 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 2457 2805 2886 3057 3084 3168 Size: 552 1080 312 348 81 171 27 84 Cuts at: 3168 513 Size: 3484 Fragments arranged by size: 3484 1080 552 348 312 171 84 81 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2428 3319 487 Size: 683 56 1202 891 3307 Fragments arranged by size: 3307 1202 891 683 56 BtsI GCAGTG_nn' Cuts at: 3655 4449 4469 3655 Size: 794 20 5325 Fragments arranged by size: 5325 794 20 ClaI AT'CG_AT Cuts at: 1554 2079 1554 Size: 525 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 3274 5146 809 Size: 507 1284 674 1872 1802 Fragments arranged by size: 1872 1802 1284 674 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2061 2259 Size: 54 1425 50 275 18 214 17 198 Cuts at: 2259 2460 2796 2818 2850 2883 2994 3060 3128 Size: 201 336 22 32 33 111 66 68 Cuts at: 3128 3257 3291 3390 3408 3428 3586 4475 6104 Size: 129 34 99 18 20 158 889 1629 Cuts at: 6104 8 Size: 43 Fragments arranged by size: 1629 1425 889 336 275 214 201 198 158 129 111 99 68 66 54 50 43 34 33 32 22 20 18 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2061 2259 2796 2818 2850 2883 2994 Size: 275 249 198 537 22 32 33 111 Cuts at: 2994 3060 3128 3390 3408 3586 1537 Size: 66 68 262 18 178 4090 Fragments arranged by size: 4090 537 275 262 249 198 178 111 68 66 33 32 22 18 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 4069 1353 Size: 753 1963 3423 Fragments arranged by size: 3423 1963 753 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 2321 2561 4726 5100 5246 Size: 1064 138 603 129 240 2165 374 146 Cuts at: 5246 387 Size: 1280 Fragments arranged by size: 2165 1280 1064 603 374 240 146 138 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2891 4202 4799 5586 5854 1239 Size: 393 1259 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 1259 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2171 2536 2171 Size: 365 5774 EcoO109I rG'GnC_Cy Cuts at: 2810 3163 3164 2810 Size: 353 1 5785 Fragments arranged by size: 5785 353 1 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2031 2704 2722 2778 2805 2906 3054 3084 Size: 353 673 18 56 27 101 148 30 Cuts at: 3084 3198 3287 5286 1678 Size: 114 89 1999 2531 Fragments arranged by size: 2531 1999 673 353 148 114 101 89 56 30 27 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 4780 4812 4854 Size: 32 135 32 1076 32 2100 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 2100 1076 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 2700 2801 3050 3080 2700 Size: 101 249 30 5759 Fragments arranged by size: 5759 249 101 30 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 3293 10 Size: 54 1425 343 630 831 2856 Fragments arranged by size: 2856 1425 831 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2456 4097 4456 4615 518 Size: 648 915 375 1641 359 159 2042 Fragments arranged by size: 2042 1641 915 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2376 2807 2876 2888 2954 2962 3031 Size: 541 229 431 69 12 66 8 69 Cuts at: 3031 3059 3065 3077 3086 3170 3307 3401 3555 Size: 28 6 12 9 84 137 94 154 Cuts at: 3555 4232 5380 5625 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 541 431 245 229 154 137 94 84 69 69 66 28 12 12 9 8 6 NaeI GCC'GGC Cuts at: 2800 3208 3432 2800 Size: 408 224 5507 Fragments arranged by size: 5507 408 224 NarI GG'CG_CC Cuts at: 2701 2802 3051 3081 2701 Size: 101 249 30 5759 Fragments arranged by size: 5759 249 101 30 NcoI C'CATG_G Cuts at: 513 1065 2457 513 Size: 552 1392 4195 Fragments arranged by size: 4195 1392 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 2798 3206 3430 2798 Size: 408 224 5507 Fragments arranged by size: 5507 408 224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 2883 2994 3060 3408 3586 2883 Size: 111 66 348 178 5436 Fragments arranged by size: 5436 348 178 111 66 NspI r_CATG'y Cuts at: 1132 1229 2041 2677 3497 5042 1132 Size: 97 812 636 820 1545 2229 Fragments arranged by size: 2229 1545 820 812 636 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 2037 2673 5038 2037 Size: 636 2365 3138 Fragments arranged by size: 3138 2365 636 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2660 2692 4208 4240 5721 5753 2660 Size: 32 1516 32 1481 32 3046 Fragments arranged by size: 3046 1516 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2810 2810 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 3163 3163 Size: 6139 PstI C_TGCA'G Cuts at: 830 2472 3030 3036 3378 3491 830 Size: 1642 558 6 342 113 3478 Fragments arranged by size: 3478 1642 558 342 113 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 3031 3031 Size: 6139 RsrII CG'GwC_CG Cuts at: 2276 2276 Size: 6139 SacII CC_GC'GG Cuts at: 2148 2808 2889 3060 3087 3171 2148 Size: 660 81 171 27 84 5116 Fragments arranged by size: 5116 660 171 84 81 27 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SanDI GG'GwC_CC Cuts at: 2810 2810 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 2468 2536 3026 3032 3374 3487 4622 Size: 219 1423 68 490 6 342 113 1135 Cuts at: 4622 5303 5494 826 Size: 681 191 1471 Fragments arranged by size: 1471 1423 1135 681 490 342 219 191 113 68 6 SfoI GGC'GCC Cuts at: 2702 2803 3052 3082 2702 Size: 101 249 30 5759 Fragments arranged by size: 5759 249 101 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 2929 2938 3206 1700 Size: 1229 9 268 4633 Fragments arranged by size: 4633 1229 268 9 SmaI CCC'GGG Cuts at: 1872 2631 3021 3265 3466 1872 Size: 759 390 244 201 4545 Fragments arranged by size: 4545 759 390 244 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 3417 4243 5144 5406 820 Size: 197 926 1474 826 901 262 1553 Fragments arranged by size: 1553 1474 926 901 826 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SrfI GCCC'GGGC Cuts at: 3021 3021 Size: 6139 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2391 1517 Size: 874 5265 StyI C'CwwG_G Cuts at: 513 1065 2250 2457 3578 513 Size: 552 1185 207 1121 3074 Fragments arranged by size: 3074 1185 1121 552 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 4166 4183 4336 4521 4966 325 Size: 1255 1731 855 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 855 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4385 6097 96 Size: 274 80 33 51 1443 2408 1712 138 Fragments arranged by size: 2408 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 2629 3019 3263 3464 1870 Size: 759 390 244 201 4545 Fragments arranged by size: 4545 759 390 244 201 XmnI GAAnn'nnTTC Cuts at: 2711 4268 2711 Size: 1557 4582 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SapI SexAI SfiI SwaI XcmI