(Circular) (Six-base) MAPSORT of: pFN21AB9593.seq Check: 2923 from: 1 to: 5695 pFN21AB9593 5695 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2897 2897 Size: 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3758 5142 5410 1632 Size: 2126 1384 268 1917 Fragments arranged by size: 2126 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2304 4594 5571 1203 Size: 1101 2290 977 1327 Fragments arranged by size: 2290 1327 1101 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2376 1184 Size: 1192 4503 AlwNI CAG_nnn'CTG Cuts at: 2020 2277 2363 2684 2767 3438 5010 2020 Size: 257 86 321 83 671 1572 2705 Fragments arranged by size: 2705 1572 671 321 257 86 83 ApaI G_GGCC'C Cuts at: 2381 2866 2381 Size: 485 5210 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 2770 3004 3230 3382 5491 1493 Size: 1277 234 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2373 3020 1870 Size: 73 81 349 647 4545 Fragments arranged by size: 4545 647 349 81 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2239 2369 3016 4471 Size: 325 217 627 246 206 130 647 1455 Cuts at: 4471 618 Size: 1842 Fragments arranged by size: 1842 1455 647 627 325 246 217 206 130 BanII G_rGCy'C Cuts at: 1391 2105 2381 2580 2622 2866 3014 1391 Size: 714 276 199 42 244 148 4072 Fragments arranged by size: 4072 714 276 244 199 148 42 BbeI G_GCGC'C Cuts at: 2037 2037 Size: 5695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BbvCI CC'TCA_GC Cuts at: 2364 2815 2364 Size: 451 5244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3886 3920 1903 Size: 34 50 34 1865 34 3678 Fragments arranged by size: 3678 1865 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2188 4306 136 Size: 107 122 71 631 605 516 2118 1525 Fragments arranged by size: 2118 1525 631 605 516 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2244 2372 2381 2540 2866 3756 4912 1790 Size: 454 128 9 159 326 890 1156 2573 Fragments arranged by size: 2573 1156 890 454 326 159 128 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2832 4355 1239 Size: 1593 1523 2579 Fragments arranged by size: 2579 1593 1523 Bpu10I CC'TnA_GC Cuts at: 2364 2495 2815 2364 Size: 131 320 5244 Fragments arranged by size: 5244 320 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 3820 4685 4983 2819 Size: 1001 865 298 3531 Fragments arranged by size: 3531 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2972 3502 3884 Size: 53 83 186 936 501 938 530 382 Cuts at: 3884 4519 275 Size: 635 1451 Fragments arranged by size: 1451 938 936 635 530 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2967 4128 4526 4800 4947 5358 2967 Size: 1161 398 274 147 411 3304 Fragments arranged by size: 3304 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2044 2074 1178 Size: 30 165 30 641 30 4799 Fragments arranged by size: 4799 641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 2539 2650 4898 5394 2539 Size: 111 2248 496 2840 Fragments arranged by size: 2840 2248 496 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2817 1163 Size: 573 1081 4041 Fragments arranged by size: 4041 1081 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2448 3145 3906 4934 1540 Size: 275 186 447 697 761 1028 2301 Fragments arranged by size: 2301 1028 761 697 447 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 2316 2462 2622 2805 3756 3841 4912 2105 Size: 211 146 160 183 951 85 1071 2888 Fragments arranged by size: 2888 1071 951 211 183 160 146 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2244 2316 2372 2381 2462 2540 Size: 399 315 139 72 56 9 81 78 Cuts at: 2540 2580 2622 2805 2866 3014 3756 3841 4912 Size: 40 42 183 61 148 742 85 1071 Cuts at: 4912 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 315 183 148 139 85 81 78 72 61 56 42 40 9 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 3050 844 Size: 2053 153 3489 Fragments arranged by size: 3489 2053 153 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 2447 2669 4339 4526 1700 Size: 110 368 269 222 1670 187 2869 Fragments arranged by size: 2869 1670 368 269 222 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 2457 794 Size: 1226 437 4032 Fragments arranged by size: 4032 1226 437 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2482 3025 3392 3777 3794 5246 5332 5344 Size: 510 543 367 385 17 1452 86 12 Cuts at: 5344 5690 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 543 510 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2405 2405 Size: 5695 BtgI C'CryG_G Cuts at: 513 1065 2395 2571 2625 513 Size: 552 1330 176 54 3583 Fragments arranged by size: 3583 1330 552 176 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2059 2262 487 Size: 683 56 833 203 3920 Fragments arranged by size: 3920 833 683 203 56 BtsI GCAGTG_nn' Cuts at: 2462 2867 3211 4005 4025 2462 Size: 405 344 794 20 4132 Fragments arranged by size: 4132 794 405 344 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2396 2396 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2429 4702 809 Size: 507 1113 2273 1802 Fragments arranged by size: 2273 1802 1113 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2564 2628 Size: 54 1425 50 275 18 261 473 64 Cuts at: 2628 3142 4031 5660 8 Size: 514 889 1629 43 Fragments arranged by size: 1629 1425 889 514 473 275 261 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 4282 4656 4802 387 Size: 1064 138 603 2090 374 146 1280 Fragments arranged by size: 2090 1280 1064 603 374 146 138 EcoICRI GAG'CTC Cuts at: 2103 2620 2103 Size: 517 5178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2832 3758 4355 5142 5410 1239 Size: 393 1200 926 597 787 268 1524 Fragments arranged by size: 1524 1200 926 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2377 2378 2422 2491 2701 2863 2377 Size: 1 44 69 210 162 5209 Fragments arranged by size: 5209 210 162 69 44 1 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2037 4842 1678 Size: 359 2805 2531 Fragments arranged by size: 2805 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373 Size: 32 135 32 2764 32 42 32 2626 Fragments arranged by size: 2764 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 2497 748 Size: 1749 3946 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2033 2033 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2630 10 Size: 54 1425 343 261 537 3075 Fragments arranged by size: 3075 1425 537 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 3653 4012 4171 518 Size: 648 2487 359 159 2042 Fragments arranged by size: 2487 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 3111 3788 4936 5181 1606 Size: 510 995 677 1148 245 2120 Fragments arranged by size: 2120 1148 995 677 510 245 NaeI GCC'GGC Cuts at: 2671 2671 Size: 5695 NarI GG'CG_CC Cuts at: 2034 2034 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 2571 513 Size: 552 1506 3637 Fragments arranged by size: 3637 1506 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2669 2669 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2308 2610 3053 4598 1132 Size: 97 1079 302 443 1545 2229 Fragments arranged by size: 2229 1545 1079 443 302 97 PasI CC'CwG_GG Cuts at: 1348 2612 2898 1348 Size: 1264 286 4145 Fragments arranged by size: 4145 1264 286 PciI A'CATG_T Cuts at: 2304 4594 2304 Size: 2290 3405 PflMI CCAn_nnn'nTGG Cuts at: 1108 2657 1108 Size: 1549 4146 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2422 2422 Size: 5695 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2377 2862 2377 Size: 485 5210 PstI C_TGCA'G Cuts at: 830 2763 3047 830 Size: 1933 284 3478 Fragments arranged by size: 3478 1933 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SacI G_AGCT'C Cuts at: 2105 2622 2105 Size: 517 5178 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2065 2759 3043 4178 4859 5050 826 Size: 219 1020 694 284 1135 681 191 1471 Fragments arranged by size: 1471 1135 1020 694 681 284 219 191 SfoI GGC'GCC Cuts at: 2035 2035 Size: 5695 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2026 2375 3022 1872 Size: 154 349 647 4545 Fragments arranged by size: 4545 647 349 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 2798 3799 4700 4962 820 Size: 197 926 855 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 855 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 2610 3053 1132 Size: 1478 443 3774 Fragments arranged by size: 3774 1478 443 SrfI GCCC'GGGC Cuts at: 2026 2026 Size: 5695 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2560 2796 1517 Size: 1043 236 4416 Fragments arranged by size: 4416 1043 236 StyI C'CwwG_G Cuts at: 513 1065 2385 2487 2571 3134 513 Size: 552 1320 102 84 563 3074 Fragments arranged by size: 3074 1320 563 552 102 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 2854 3722 3739 3892 4077 4522 Size: 1255 1004 270 868 17 153 185 445 Cuts at: 4522 325 Size: 1498 Fragments arranged by size: 1498 1255 1004 868 445 270 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2024 2373 3020 1870 Size: 154 349 647 4545 Fragments arranged by size: 4545 647 349 154 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SwaI XcmI