(Circular) (Six-base) MAPSORT of: pFN21AB9590.seq Check: 6713 from: 1 to: 5920 pFN21AB9590 5920 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3730 278 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3263 1937 Size: 1326 4594 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 4047 4420 1563 Size: 2484 373 3063 Fragments arranged by size: 3063 2484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 3983 5367 5635 1632 Size: 417 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 417 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 4819 5796 1203 Size: 3616 977 1327 Fragments arranged by size: 3616 1327 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 4204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5920 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2255 2287 2255 Size: 32 5888 AlwNI CAG_nnn'CTG Cuts at: 3663 5235 3663 Size: 1572 4348 ApaLI G'TGCA_C Cuts at: 3977 5133 3977 Size: 1156 4764 ApoI r'AATT_y Cuts at: 1493 2959 3229 3455 3607 5716 1493 Size: 1466 270 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 270 226 152 AseI AT'TA_AT Cuts at: 160 1032 4474 160 Size: 872 3442 1606 Fragments arranged by size: 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 2114 3245 1870 Size: 73 171 1131 4545 Fragments arranged by size: 4545 1131 171 73 BamHI G'GATC_C Cuts at: 3250 3617 3250 Size: 367 5553 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3241 4696 618 Size: 325 217 627 1454 1455 1842 Fragments arranged by size: 1842 1455 1454 627 325 217 BanII G_rGCy'C Cuts at: 1391 3239 1391 Size: 1848 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2801 2946 928 Size: 971 902 145 3902 Fragments arranged by size: 3902 971 902 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3079 3113 4111 4145 1903 Size: 34 1142 34 998 34 3678 Fragments arranged by size: 3678 1142 998 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 1548 Size: 5920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4531 136 Size: 107 122 71 631 605 2859 1525 Fragments arranged by size: 2859 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5915 5915 Size: 5920 BlpI GC'TnA_GC Cuts at: 3337 3337 Size: 5920 Bme1580I G_kGCm'C Cuts at: 1790 2769 3981 5137 1790 Size: 979 1212 1156 2573 Fragments arranged by size: 2573 1212 1156 979 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 4765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3651 4609 442 Size: 416 53 83 399 24 2234 958 1753 Fragments arranged by size: 2234 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2521 2999 3121 4580 1239 Size: 1282 478 122 1459 2579 Fragments arranged by size: 2579 1459 1282 478 122 Bpu10I CC'TnA_GC Cuts at: 3003 3003 Size: 5920 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 4045 4910 5208 2030 Size: 2015 865 298 2742 Fragments arranged by size: 2742 2015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 3140 4583 882 Size: 617 689 952 1443 2219 Fragments arranged by size: 2219 1443 952 689 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 2989 3057 3711 493 Size: 711 1560 225 68 654 2702 Fragments arranged by size: 2702 1560 711 654 225 68 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3727 4109 4744 275 Size: 53 83 186 936 2194 382 635 1451 Fragments arranged by size: 2194 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3113 4353 4751 5025 5172 5583 3113 Size: 1240 398 274 147 411 3450 Fragments arranged by size: 3450 1240 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3119 3149 1178 Size: 30 165 30 1716 30 3949 Fragments arranged by size: 3949 1716 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2269 2735 3176 3185 3188 1186 Size: 1083 466 441 9 3 3918 Fragments arranged by size: 3918 1083 466 441 9 3 BseYI C'CCAG_C Cuts at: 2240 3151 5123 5619 2240 Size: 911 1972 496 2541 Fragments arranged by size: 2541 1972 911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 1163 Size: 573 654 4693 Fragments arranged by size: 4693 654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2741 3370 4131 5159 1540 Size: 275 186 740 629 761 1028 2301 Fragments arranged by size: 2301 1028 761 740 629 275 186 BsiHKAI G_wGCw'C Cuts at: 3981 4066 5137 3981 Size: 85 1071 4764 Fragments arranged by size: 4764 1071 85 BsiWI C'GTAC_G Cuts at: 2985 2985 Size: 5920 BsmI GAATG_Cn' Cuts at: 2269 3431 3524 2269 Size: 1162 93 4665 Fragments arranged by size: 4665 1162 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2357 2357 Size: 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2769 3239 3981 4066 5137 1391 Size: 399 979 470 742 85 1071 2174 Fragments arranged by size: 2174 1071 979 742 470 399 85 BspEI T'CCGG_A Cuts at: 5583 5583 Size: 5920 BspHI T'CATG_A Cuts at: 3809 5539 3809 Size: 1730 4190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2716 3026 3275 844 Size: 1872 310 249 3489 Fragments arranged by size: 3489 1872 310 249 BsrBI CCG'CTC Cuts at: 1394 1823 2113 3807 1394 Size: 429 290 1694 3507 Fragments arranged by size: 3507 1694 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 3084 4415 4589 65 Size: 1183 1836 1331 174 1396 Fragments arranged by size: 1836 1396 1331 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4564 4751 1700 Size: 110 2754 187 2869 Fragments arranged by size: 2869 2754 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 3980 4992 3980 Size: 1012 4908 BstAPI GCAn_nnn'nTGC Cuts at: 794 2128 794 Size: 1334 4586 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5920 BstXI CCAn_nnnn'nTGG Cuts at: 2962 2962 Size: 5920 BstYI r'GATC_y Cuts at: 1972 3119 3250 3617 4002 4019 5471 5557 5569 Size: 1147 131 367 385 17 1452 86 12 Cuts at: 5569 5915 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1147 385 367 346 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 513 Size: 552 939 78 4351 Fragments arranged by size: 4351 939 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5181 Fragments arranged by size: 5181 683 56 BtsI GCAGTG_nn' Cuts at: 3436 4230 4250 3436 Size: 794 20 5106 Fragments arranged by size: 5106 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3224 3224 Size: 5920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4927 809 Size: 507 3611 1802 Fragments arranged by size: 3611 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2520 2738 Size: 54 1425 50 275 18 255 435 218 Cuts at: 2738 2952 3102 3367 4256 5885 8 Size: 214 150 265 889 1629 43 Fragments arranged by size: 1629 1425 889 435 275 265 255 218 214 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2738 3367 1537 Size: 275 926 629 4090 Fragments arranged by size: 4090 926 629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2615 3162 3850 1353 Size: 1262 547 688 3423 Fragments arranged by size: 3423 1262 688 547 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4507 4881 5027 387 Size: 1064 138 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2521 2999 3121 3983 4580 5367 Size: 393 417 472 478 122 862 597 787 Cuts at: 5367 5635 1239 Size: 268 1524 Fragments arranged by size: 1524 862 787 597 478 472 417 393 268 122 EcoNI CCTnn'n_nnAGG Cuts at: 2220 2220 Size: 5920 EcoRI G'AATT_C Cuts at: 3229 3607 3229 Size: 378 5542 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 5920 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 5067 1678 Size: 3389 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 4561 4593 4635 Size: 32 135 32 1241 32 1716 32 42 Cuts at: 4635 4667 1373 Size: 32 2626 Fragments arranged by size: 2626 1716 1241 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2158 3264 3510 669 Size: 988 281 220 1106 246 3079 Fragments arranged by size: 3079 1106 988 281 246 220 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 5034 5218 1305 Size: 1539 2190 184 2007 Fragments arranged by size: 2190 2007 1539 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 2954 3104 10 Size: 54 1425 343 690 432 150 2826 Fragments arranged by size: 2826 1425 690 432 343 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2762 2882 3878 4237 4396 518 Size: 648 1050 546 120 996 359 159 2042 Fragments arranged by size: 2042 1050 996 648 546 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2737 2743 3336 4013 5161 5406 1606 Size: 1131 6 593 677 1148 245 2120 Fragments arranged by size: 2120 1148 1131 677 593 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4429 Fragments arranged by size: 4429 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5920 NotI GC'GGCC_GC Cuts at: 2738 3367 2738 Size: 629 5291 NspI r_CATG'y Cuts at: 1132 1229 3278 4823 1132 Size: 97 2049 1545 2229 Fragments arranged by size: 2229 2049 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5920 PciI A'CATG_T Cuts at: 4819 4819 Size: 5920 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 2401 2716 1108 Size: 1065 228 315 4312 Fragments arranged by size: 4312 1065 315 228 PfoI T'CCnGG_A Cuts at: 2921 2921 Size: 5920 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PmlI CAC'GTG Cuts at: 2764 3057 2764 Size: 293 5627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3989 4021 5502 5534 3989 Size: 32 1481 32 4375 Fragments arranged by size: 4375 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5920 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PstI C_TGCA'G Cuts at: 830 2813 3272 830 Size: 1983 459 3478 Fragments arranged by size: 3478 1983 459 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 SalI G'TCGA_C Cuts at: 1936 3262 1936 Size: 1326 4594 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SexAI A'CCwGG_T Cuts at: 2694 2694 Size: 5920 SfcI C'TryA_G Cuts at: 826 1045 2672 2809 3268 4403 5084 5275 826 Size: 219 1627 137 459 1135 681 191 1471 Fragments arranged by size: 1627 1471 1135 681 459 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 3247 1872 Size: 1375 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 4024 4925 5187 820 Size: 197 926 102 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 3278 1132 Size: 2146 3774 SspI AAT'ATT Cuts at: 5 52 3844 5 Size: 47 3792 2081 Fragments arranged by size: 3792 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5920 StyI C'CwwG_G Cuts at: 513 1065 2004 2288 3359 513 Size: 552 939 284 1071 3074 Fragments arranged by size: 3074 1071 939 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 3947 3964 4117 4302 4747 325 Size: 1255 674 1693 17 153 185 445 1498 Fragments arranged by size: 1693 1498 1255 674 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3126 4166 5878 Size: 274 80 33 51 1443 1149 1040 1712 Cuts at: 5878 96 Size: 138 Fragments arranged by size: 1712 1443 1149 1040 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5880 5912 5880 Size: 32 5888 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5920 XbaI T'CTAG_A Cuts at: 3256 3256 Size: 5920 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 5920 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 3245 1870 Size: 1375 4545 XmnI GAAnn'nnTTC Cuts at: 4049 4049 Size: 5920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3728 276 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI