(Circular) (Six-base) MAPSORT of: pFN21AB9587.seq Check: 5168 from: 1 to: 5413 pFN21AB9587 5413 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2293 2293 Size: 5413 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 2497 2756 1937 Size: 130 430 259 4594 Fragments arranged by size: 4594 430 259 130 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 3476 4860 5128 1632 Size: 410 1434 1384 268 1917 Fragments arranged by size: 1917 1434 1384 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 2099 1166 Size: 933 4480 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2489 2576 2597 2686 3156 4728 2489 Size: 87 21 89 470 1572 3174 Fragments arranged by size: 3174 1572 470 89 87 21 ApaI G_GGCC'C Cuts at: 2483 2483 Size: 5413 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2738 1870 Size: 73 795 4545 Fragments arranged by size: 4545 795 73 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2199 2248 2462 2566 Size: 325 217 627 323 89 49 214 104 Cuts at: 2566 2734 4189 618 Size: 168 1455 1842 Fragments arranged by size: 1842 1455 627 325 323 217 214 168 104 89 49 BanII G_rGCy'C Cuts at: 1391 2483 2732 1391 Size: 1092 249 4072 Fragments arranged by size: 4072 1092 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4442 BbvCI CC'TCA_GC Cuts at: 2369 2369 Size: 5413 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3604 3638 1903 Size: 34 50 34 1583 34 3678 Fragments arranged by size: 3678 1583 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2348 4024 136 Size: 107 122 71 631 605 676 1676 1525 Fragments arranged by size: 1676 1525 676 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2009 2830 2009 Size: 821 4592 Bme1580I G_kGCm'C Cuts at: 1790 2483 2569 2678 3474 4630 1790 Size: 693 86 109 796 1156 2573 Fragments arranged by size: 2573 1156 796 693 109 86 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2289 3144 4102 Size: 416 53 83 399 24 872 855 958 Cuts at: 4102 442 Size: 1753 Fragments arranged by size: 1753 958 872 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2412 2412 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2708 4073 1239 Size: 1142 327 1365 2579 Fragments arranged by size: 2579 1365 1142 327 Bpu10I CC'TnA_GC Cuts at: 2369 2369 Size: 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 2534 3538 4403 4701 2141 Size: 393 1004 865 298 2853 Fragments arranged by size: 2853 1004 865 393 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2240 3220 3602 4237 Size: 53 83 186 936 707 980 382 635 Cuts at: 4237 275 Size: 1451 Fragments arranged by size: 1451 980 936 707 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2626 3846 4244 4518 4665 5076 2626 Size: 1220 398 274 147 411 2963 Fragments arranged by size: 2963 1220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5188 Fragments arranged by size: 5188 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2057 2060 1186 Size: 865 3 3 3 4539 Fragments arranged by size: 4539 865 3 3 3 BseYI C'CCAG_C Cuts at: 2453 2476 2482 4616 5112 2453 Size: 23 6 2134 496 2754 Fragments arranged by size: 2754 2134 496 23 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4840 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 2696 2863 3624 4652 1540 Size: 275 186 120 575 167 761 1028 2301 Fragments arranged by size: 2301 1028 761 575 275 186 167 120 BsiHKAI G_wGCw'C Cuts at: 2157 3474 3559 4630 2157 Size: 1317 85 1071 2940 Fragments arranged by size: 2940 1317 1071 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2483 2569 2678 2732 3474 3559 Size: 399 367 326 86 109 54 742 85 Cuts at: 3559 4630 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 326 109 86 85 54 BspEI T'CCGG_A Cuts at: 2626 5076 2626 Size: 2450 2963 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2293 2481 2768 844 Size: 1358 91 188 287 3489 Fragments arranged by size: 3489 1358 287 188 91 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4057 4244 1700 Size: 110 2247 187 2869 Fragments arranged by size: 2869 2247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 4001 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2359 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2083 2743 3110 3495 3512 4964 5050 5062 Size: 111 660 367 385 17 1452 86 12 Cuts at: 5062 5408 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 660 385 367 346 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2598 2598 Size: 5413 BtgI C'CryG_G Cuts at: 513 1065 2121 2244 2253 2346 513 Size: 552 1056 123 9 93 3580 Fragments arranged by size: 3580 1056 552 123 93 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DraIII CAC_nnn'GTG Cuts at: 2206 2206 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2118 2349 Size: 54 1425 50 275 18 259 29 231 Cuts at: 2349 2860 3749 5378 8 Size: 511 889 1629 43 Fragments arranged by size: 1629 1425 889 511 275 259 231 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 2860 1537 Size: 275 306 742 4090 Fragments arranged by size: 4090 742 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3343 1353 Size: 1990 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2381 2708 3476 4073 4860 5128 Size: 393 410 339 327 768 597 787 268 Cuts at: 5128 1239 Size: 1524 Fragments arranged by size: 1524 787 768 597 410 393 339 327 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2390 2525 2360 Size: 30 135 5248 Fragments arranged by size: 5248 135 30 EcoO109I rG'GnC_Cy Cuts at: 2365 2383 2518 2581 2594 2365 Size: 18 135 63 13 5184 Fragments arranged by size: 5184 135 63 18 13 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 2453 4560 1678 Size: 775 2107 2531 Fragments arranged by size: 2531 2107 775 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 4054 4086 4128 Size: 32 135 32 430 32 2020 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 2020 430 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 2757 3003 669 Size: 988 281 130 689 246 3079 Fragments arranged by size: 3079 988 689 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 2351 10 Size: 54 1425 343 259 260 3072 Fragments arranged by size: 3072 1425 343 260 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2543 2663 3371 3730 3889 518 Size: 648 933 444 120 708 359 159 2042 Fragments arranged by size: 2042 933 708 648 444 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2099 2255 2829 3506 4654 4899 1606 Size: 493 156 574 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 574 493 245 156 NcoI C'CATG_G Cuts at: 513 1065 2244 2346 513 Size: 552 1179 102 3580 Fragments arranged by size: 3580 1179 552 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 2489 1108 Size: 1299 82 4032 Fragments arranged by size: 4032 1299 82 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2365 Size: 5413 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PspOMI G'GGCC_C Cuts at: 2479 2479 Size: 5413 PstI C_TGCA'G Cuts at: 830 2178 2290 2765 830 Size: 1348 112 475 3478 Fragments arranged by size: 3478 1348 475 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SacII CC_GC'GG Cuts at: 2256 2256 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2066 2755 1936 Size: 130 689 4594 Fragments arranged by size: 4594 689 130 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SexAI A'CCwGG_T Cuts at: 2202 2202 Size: 5413 SfcI C'TryA_G Cuts at: 826 1045 2174 2286 2761 3896 4577 4768 826 Size: 219 1129 112 475 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 681 475 219 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 2513 3517 4418 4680 820 Size: 197 926 213 357 1004 901 262 1553 Fragments arranged by size: 1553 1004 926 901 357 262 213 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2244 2346 2406 2466 2852 513 Size: 552 1179 102 60 60 386 3074 Fragments arranged by size: 3074 1179 552 386 102 60 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 3440 3457 3610 3795 4240 325 Size: 1255 870 990 17 153 185 445 1498 Fragments arranged by size: 1498 1255 990 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3659 5371 96 Size: 274 80 33 51 1443 1682 1712 138 Fragments arranged by size: 1712 1682 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5373 5405 5373 Size: 32 5381 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI