Restriction map for
pFN21AB9581
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeII MamI
MboI MfeI MflI MluI MluNI
MscI MseI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 778 TCCGGA T^CCGGA
AfaI 852 GTAC GT^AC
AlwNI 836 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 437 GGGCCC GGGCC^C
AvaII 998 GGTCC G^G[AT]CC
AviII 545 TGCGCA TGC^GCA
BfaI 484 CTAG C^TAG
BlpI 766 GCTCAGC GC^T[ACGT]AGC
BpmI 680 CTCCAG CTGGAG(16/14)
Bpu1102I 766 GCTCAGC GC^T[ACGT]AGC
BsaWI 778 TCCGGA [AT]^CCGG[AT]
BseAI 778 TCCGGA T^CCGGA
BseRI 321 GAGGAG GAGGAG(10/8)
BsmBI 477 GAGACG CGTCTC(1/5)
BspEI 778 TCCGGA T^CCGGA
BspMI 111 ACCTGC ACCTGC(4/8)
BsrBI 502 GAGCGG CCGCTC(-3/-3)
BsrDI 548 GCAATG GCAATG(2/0)
BsrFI 360 GCCGGT [GA]^CCGG[CT]
BstAPI 960 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 181 CCACGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 766 GCTCAGC GC^T[ACGT]AGC
Cfr10I 360 GCCGGT [GA]^CCGG[CT]
DraII 437 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 719 CCGCGG C^C[GA][CT]GG
EarI 22 GAAGAG CTCTTC(1/4)
EcoO109I 437 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 514 ATGCAT ATGCA^T
FspI 545 TGCGCA TGC^GCA
HaeII 898 AGCGCC [GA]GCGC^[CT]
HgaI 868 GACGC GACGC(5/10)
Ksp632I 22 GAAGAG CTCTTC(1/4)
KspI 719 CCGCGG CCGC^GG
MaeI 484 CTAG C^TAG
MaeIII 1043 GTGAC ^GT[ACGT]AC
MboII 22 GAAGA GAAGA(8/7)
MroI 778 TCCGGA T^CCGGA
MslI 1036 CATTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 483 GCTAGC G^CTAGC
NsiI 514 ATGCAT ATGCA^T
NspI 15 GCATGC [GA]CATG^[CT]
RsaI 852 GTAC GT^AC
SacII 719 CCGCGG CCGC^GG
SphI 15 GCATGC GCATG^C
Tsp45I 1043 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 142 GTCTAC GT^[AC][GT]AC
409 GTAGAC
AcsI 927 GAATTT [GA]^AATT[CT]
1018 GAATTT
ApoI 927 GAATTT [GA]^AATT[CT]
1018 GAATTT
BanI 262 GGTGCC G^G[CT][GA]CC
334 GGTGCC
BglI 187 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
265 GCCTCAGAGGC
BsaI 50 GAGACC GGTCTC(1/5)
595 GAGACC
BsrI 234 CCAGT ACTGG(1/-1)
964 CCAGT
EaeI 300 CGGCCG [CT]^GGCC[GA]
793 CGGCCG
EagI 300 CGGCCG C^GGCCG
793 CGGCCG
EclXI 300 CGGCCG C^GGCCG
793 CGGCCG
Eco52I 300 CGGCCG C^GGCCG
793 CGGCCG
HinfI 819 GACTC G^A[ACGT]TC
1045 GACTC
HphI 363 GGTGA GGTGA(8/7)
456 GGTGA
NlaIII 16 CATG CATG^
517 CATG
PleI 819 GACTC GAGTC(4/5)
1045 GACTC
PstI 423 CTGCAG CTGCA^G
601 CTGCAG
SfcI 423 CTGCAG C^T[GA][CT]AG
601 CTGCAG
Tsp509I 928 AATT ^AATT
1019 AATT
TspRI 965 CAGTG CA[GC]TG[ACGT][ACGT]^
1041 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV PvuII Sau96I
ScrFI SfaNI SmaI TaqI TseI
TthHB8I XmaCI XmaI