Restriction map for
pFN21AB9581
(Linear) (Six-base) MAPSORT of: KIBB9581.seq Check: 8233 from: 1 to: 1062
KIBB9581.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 120 1062
Size: 120 942
AccI GT'mk_AC
Cuts at: 0 143 410 1062
Size: 143 267 652
Fragments arranged by size:
652 267 143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1062
Size: 53 1009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 1062
Size: 128 934
AlwNI CAG_nnn'CTG
Cuts at: 0 841 1062
Size: 841 221
ApaI G_GGCC'C
Cuts at: 0 441 1062
Size: 441 621
ApoI r'AATT_y
Cuts at: 0 927 1018 1062
Size: 927 91 44
Fragments arranged by size:
927 91 44
AvaI C'yCGr_G
Cuts at: 0 284 355 805 946 1062
Size: 284 71 450 141 116
Fragments arranged by size:
450 284 141 116 71
BanI G'GyrC_C
Cuts at: 0 262 334 1062
Size: 262 72 728
Fragments arranged by size:
728 262 72
BanII G_rGCy'C
Cuts at: 0 355 441 768 1062
Size: 355 86 327 294
Fragments arranged by size:
355 327 294 86
BfrBI ATG'CAT
Cuts at: 0 516 1062
Size: 516 546
BglI GCCn_nnn'nGGC
Cuts at: 0 193 271 1062
Size: 193 78 791
Fragments arranged by size:
791 193 78
BlpI GC'TnA_GC
Cuts at: 0 767 1062
Size: 767 295
Bme1580I G_kGCm'C
Cuts at: 0 441 1062
Size: 441 621
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 1062
Size: 228 834
BmtI G_CTAG'C
Cuts at: 0 487 1062
Size: 487 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1062
Size: 665 397
Bpu10I CC'TnA_GC
Cuts at: 0 152 1062
Size: 152 910
BsaI GGTCTCn'nnnn_
Cuts at: 0 44 589 1062
Size: 44 545 473
Fragments arranged by size:
545 473 44
BsaWI w'CCGG_w
Cuts at: 0 778 1062
Size: 778 284
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 1062
Size: 336 726
BseYI C'CCAG_C
Cuts at: 0 68 258 417 652 760 834 1062
Size: 68 190 159 235 108 74 228
Fragments arranged by size:
235 228 190 159 108 74 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 487 859 880 988 1062
Size: 201 286 372 21 108 74
Fragments arranged by size:
372 286 201 108 74 21
BsiEI CG_ry'CG
Cuts at: 0 303 755 796 1062
Size: 303 452 41 266
Fragments arranged by size:
452 303 266 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 471 1062
Size: 471 591
Bsp1286I G_dGCh'C
Cuts at: 0 355 441 768 1062
Size: 355 86 327 294
Fragments arranged by size:
355 327 294 86
BspEI T'CCGG_A
Cuts at: 0 778 1062
Size: 778 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 120 1062
Size: 120 942
BsrBI CCG'CTC
Cuts at: 0 504 1062
Size: 504 558
BsrDI GCAATG_nn'
Cuts at: 0 555 1062
Size: 555 507
BsrFI r'CCGG_y
Cuts at: 0 360 1062
Size: 360 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 966 1062
Size: 966 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 188 1062
Size: 188 874
BtgI C'CryG_G
Cuts at: 0 719 1062
Size: 719 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 43 925 1062
Size: 43 882 137
Fragments arranged by size:
882 137 43
EaeI y'GGCC_r
Cuts at: 0 300 793 1062
Size: 300 493 269
Fragments arranged by size:
493 300 269
EagI C'GGCC_G
Cuts at: 0 300 793 1062
Size: 300 493 269
Fragments arranged by size:
493 300 269
EarI CTCTTCn'nnn_
Cuts at: 0 17 1062
Size: 17 1045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 665 1062
Size: 53 612 397
Fragments arranged by size:
612 397 53
EcoO109I rG'GnC_Cy
Cuts at: 0 438 1062
Size: 438 624
FspI TGC'GCA
Cuts at: 0 547 1062
Size: 547 515
HaeII r_GCGC'y
Cuts at: 0 902 1062
Size: 902 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 1062
Size: 120 32 910
Fragments arranged by size:
910 120 32
MslI CAynn'nnrTG
Cuts at: 0 1040 1062
Size: 1040 22
MspA1I CmG'CkG
Cuts at: 0 277 329 381 543 656 721 760 792
Size: 277 52 52 162 113 65 39 32
Cuts at: 792 864 1062
Size: 72 198
Fragments arranged by size:
277 198 162 113 72 65 52 52
39 32
NheI G'CTAG_C
Cuts at: 0 483 1062
Size: 483 579
NsiI A_TGCA'T
Cuts at: 0 518 1062
Size: 518 544
NspI r_CATG'y
Cuts at: 0 19 1062
Size: 19 1043
PfoI T'CCnGG_A
Cuts at: 0 164 1062
Size: 164 898
PspOMI G'GGCC_C
Cuts at: 0 437 1062
Size: 437 625
PstI C_TGCA'G
Cuts at: 0 427 605 1062
Size: 427 178 457
Fragments arranged by size:
457 427 178
PvuII CAG'CTG
Cuts at: 0 381 543 760 864 1062
Size: 381 162 217 104 198
Fragments arranged by size:
381 217 198 162 104
SacII CC_GC'GG
Cuts at: 0 722 1062
Size: 722 340
SfcI C'TryA_G
Cuts at: 0 423 601 1062
Size: 423 178 461
Fragments arranged by size:
461 423 178
SmaI CCC'GGG
Cuts at: 0 286 357 807 948 1062
Size: 286 71 450 141 114
Fragments arranged by size:
450 286 141 114 71
SphI G_CATG'C
Cuts at: 0 19 1062
Size: 19 1043
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 986 1062
Size: 250 736 76
Fragments arranged by size:
736 250 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 1062
Size: 367 32 663
Fragments arranged by size:
663 367 32
XmaI C'CCGG_G
Cuts at: 0 284 355 805 946 1062
Size: 284 71 450 141 116
Fragments arranged by size:
450 284 141 116 71
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BfrBI BglI BlpI Bme1580I BmrI BmtI
BpmI Bpu10I BsaI BsaWI BseRI BseYI BsgI BsiEI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BtgI BtgZI EaeI EagI EarI Eco57MI EcoO109I
FspI HaeII Hin4I MslI MspA1I NheI NsiI NspI
PfoI PspOMI PstI PvuII SacII SfcI SmaI SphI
TaqII TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BglII
BmgBI BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsiHKAI
BsiWI BsmI BspHI BsrGI BssHII BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI Tth111I XbaI XcmI XhoI XmnI
ZraI