Restriction map for
pFN21AB9577
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII BbeI
BbvCI BciVI BclI BglII Bsp1407I
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CpoI DraIII Eam1105I Eco47III
Eco81I EcoRV EcoT22I FbaI FseI
FspI KasI KpnI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 2570 TCCGGA T^CCGGA
AflII 1674 CTTAAG C^TTAAG
AgeI 2915 ACCGGT A^CCGGT
Asp700I 3033 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 55 CCTAGG C^CTAGG
BalI 2721 TGGCCA TGG^CCA
BamHI 485 GGATCC G^GATCC
BcgI 2973 CGAGGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1674 CTTAAG C^TTAAG
BlnI 55 CCTAGG C^CTAGG
BsaBI 2574 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2570 TCCGGA T^CCGGA
BsiWI 3324 CGTACG C^GTACG
BsmI 2683 GAATGC GAATGC(1/-1)
BspDI 3356 ATCGAT AT^CGAT
BspEI 2570 TCCGGA T^CCGGA
BspLU11I 1577 ACATGT A^CATGT
BsrBI 1662 GAGCGG CCGCTC(-3/-3)
BssHII 1366 GCGCGC G^CGCGC
BstAPI 3540 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2997 GGTGACC G^GT[ACGT]ACC
BstPI 2997 GGTGACC G^GT[ACGT]ACC
ClaI 3356 ATCGAT AT^CGAT
DraI 1761 TTTAAA TTT^AAA
EagI 3110 CGGCCG C^GGCCG
EarI 735 GAAGAG CTCTTC(1/4)
EclXI 3110 CGGCCG C^GGCCG
Eco52I 3110 CGGCCG C^GGCCG
EcoO65I 2997 GGTGACC G^GT[ACGT]ACC
HaeII 714 GGCGCT [GA]GCGC^[CT]
HincII 1410 GTTAAC GT[CT]^[GA]AC
HindII 1410 GTTAAC GT[CT]^[GA]AC
HpaI 1410 GTTAAC GTT^AAC
Ksp632I 735 GAAGAG CTCTTC(1/4)
MamI 2574 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2721 TGGCCA TGG^CCA
MroI 2570 TCCGGA T^CCGGA
MscI 2721 TGGCCA TGG^CCA
NaeI 2460 GCCGGC GCC^GGC
NgoMIV 2460 GCCGGC G^CCGGC
PaeR7I 2971 CTCGAG C^TCGAG
PflMI 960 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2915 ACCGGT A^CCGGT
PshAI 2278 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2489 CCTGCAGG CCTGCA^GG
Sse8387I 2489 CCTGCAGG CCTGCA^GG
SspI 1039 AATATT AAT^ATT
StuI 1729 AGGCCT AGG^CCT
Van91I 960 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2971 CTCGAG C^TCGAG
XmnI 3033 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AseI 1042 ATTAAT AT^TAAT
1846 ATTAAT
AsnI 1042 ATTAAT AT^TAAT
1846 ATTAAT
AspHI 85 GAGCTC G[AT]GC[AT]^C
1279 GAGCTC
BanI 2148 GGCACC G^G[CT][GA]CC
3591 GGTGCC
BbrPI 498 CACGTG CAC^GTG
2644 CACGTG
BbsI 307 GAAGAC GAAGAC(2/6)
2545 GAAGAC
BglI 1200 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2943 GCCCTCACGGC
BlpI 3 GCTGAGC GC^T[ACGT]AGC
2095 GCTGAGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
2095 GCTGAGC
BpuAI 307 GAAGAC GAAGAC(2/6)
2545 GAAGAC
BsaI 2741 GAGACC GGTCTC(1/5)
3461 GAGACC
BsgI 2437 CTGCAC GTGCAG(16/14)
3533 CTGCAC
BsiEI 3110 CGGCCG CG[GA][CT]^CG
3320 CGACCG
BsiHKAI 85 GAGCTC G[AT]GC[AT]^C
1279 GAGCTC
BsmBI 743 GAGACG CGTCTC(1/5)
2537 GAGACG
BspHI 759 TCATGA T^CATGA
1821 TCATGA
BstXI 17 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1375 CCATTTTCTTGG
BstYI 485 GGATCC [GA]^GATC[CT]
1689 GGATCT
CelII 3 GCTGAGC GC^T[ACGT]AGC
2095 GCTGAGC
DrdI 3184 GACATTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3321 GACCGTACGGTC
EcoNI 378 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3028 CCTAAGAAAGG
EcoRI 1023 GAATTC G^AATTC
3230 GAATTC
HindIII 1390 AAGCTT A^AGCTT
3419 AAGCTT
MflI 485 GGATCC [GA]^GATC[CT]
1689 GGATCT
PmaCI 498 CACGTG CAC^GTG
2644 CACGTG
PmlI 498 CACGTG CAC^GTG
2644 CACGTG
PshBI 1042 ATTAAT AT^TAAT
1846 ATTAAT
PvuII 73 CAGCTG CAG^CTG
915 CAGCTG
RcaI 759 TCATGA T^CATGA
1821 TCATGA
SacI 85 GAGCTC GAGCT^C
1279 GAGCTC
XcmI 2091 CCAAGCTGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2774 CCAGACTCGCCATGG
XhoII 485 GGATCC [GA]^GATC[CT]
1689 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I