(Circular) (Six-base) MAPSORT of: pFN21AB9577.seq Check: 4297 from: 1 to: 8338 pFN21AB9577 8338 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3740 4502 5209 3740 Size: 762 707 6869 Fragments arranged by size: 6869 762 707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6148 278 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5681 1937 Size: 3744 4594 Acc65I G'GTAC_C Cuts at: 5659 5659 Size: 8338 AclI AA'CG_TT Cuts at: 1563 6465 6838 1563 Size: 4902 373 3063 Fragments arranged by size: 4902 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 6401 7785 8053 1632 Size: 1187 3582 1384 268 1917 Fragments arranged by size: 3582 1917 1384 1187 268 AflII C'TTAA_G Cuts at: 820 1017 3679 820 Size: 197 2662 5479 Fragments arranged by size: 5479 2662 197 AflIII A'CryG_T Cuts at: 1203 3582 3949 4650 4806 7237 8214 1203 Size: 2379 367 701 156 2431 977 1327 Fragments arranged by size: 2431 2379 1327 977 701 367 156 AgeI A'CCGG_T Cuts at: 4920 7169 4920 Size: 2249 6089 AhdI GACnn_n'nnGTC Cuts at: 7067 7067 Size: 8338 AleI CACnn'nnGTG Cuts at: 1166 2525 3963 4649 5000 1166 Size: 1359 1438 686 351 4504 Fragments arranged by size: 4504 1438 1359 686 351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8338 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3939 3971 4907 4939 3939 Size: 32 936 32 7338 Fragments arranged by size: 7338 936 32 32 AlwNI CAG_nnn'CTG Cuts at: 3200 5134 5551 6081 7653 3200 Size: 1934 417 530 1572 3885 Fragments arranged by size: 3885 1934 1572 530 417 ApaLI G'TGCA_C Cuts at: 6395 7551 6395 Size: 1156 7182 ApoI r'AATT_y Cuts at: 1493 3028 3860 3899 4191 5235 5647 5873 6025 Size: 1535 832 39 292 1044 412 226 152 Cuts at: 6025 8134 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1535 1044 832 412 292 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 3048 3852 6892 160 Size: 872 2016 804 3040 1606 Fragments arranged by size: 3040 2016 1606 872 804 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2355 4976 5663 1870 Size: 73 144 268 2621 687 4545 Fragments arranged by size: 4545 2621 687 268 144 73 AvrII C'CTAG_G Cuts at: 2060 2060 Size: 8338 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4826 4859 5288 5321 4826 Size: 33 429 33 7843 Fragments arranged by size: 7843 429 33 33 BamHI G'GATC_C Cuts at: 2490 5668 6035 2490 Size: 3178 367 4793 Fragments arranged by size: 4793 3178 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4153 5596 5659 7114 618 Size: 325 217 627 2366 1443 63 1455 1842 Fragments arranged by size: 2366 1842 1455 1443 627 325 217 63 BanII G_rGCy'C Cuts at: 1391 2088 2094 2457 3288 5634 5657 1391 Size: 697 6 363 831 2346 23 4072 Fragments arranged by size: 4072 2346 831 697 363 23 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2319 4557 928 Size: 971 420 2238 4709 Fragments arranged by size: 4709 2238 971 420 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4967 5001 6529 6563 1903 Size: 34 50 34 2946 34 1528 34 3678 Fragments arranged by size: 3678 2946 1528 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6230 7440 6230 Size: 1210 7128 BclI T'GATC_A Cuts at: 1548 1548 Size: 8338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3211 4954 6949 Size: 107 122 71 631 605 1539 1743 1995 Cuts at: 6949 136 Size: 1525 Fragments arranged by size: 1995 1743 1539 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8333 8333 Size: 8338 BlpI GC'TnA_GC Cuts at: 2009 4101 5755 2009 Size: 2092 1654 4592 Fragments arranged by size: 4592 2092 1654 Bme1580I G_kGCm'C Cuts at: 1790 2993 4641 6399 7555 1790 Size: 1203 1648 1758 1156 2573 Fragments arranged by size: 2573 1758 1648 1203 1156 BmgBI CAC'GTC Cuts at: 1318 2668 3952 1318 Size: 1350 1284 5704 Fragments arranged by size: 5704 1350 1284 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2479 4226 4760 Size: 416 53 83 399 24 1062 1747 534 Cuts at: 4760 5585 6069 7027 442 Size: 825 484 958 1753 Fragments arranged by size: 1753 1747 1062 958 825 534 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3756 3756 Size: 8338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2102 2170 2450 2734 3111 3207 3728 Size: 823 40 68 280 284 377 96 521 Cuts at: 3728 5152 6998 1239 Size: 1424 1846 2579 Fragments arranged by size: 2579 1846 1424 823 521 377 284 280 96 68 40 Bpu10I CC'TnA_GC Cuts at: 3201 4603 3201 Size: 1402 6936 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3448 3644 3759 6463 7328 7626 3448 Size: 196 115 2704 865 298 4160 Fragments arranged by size: 4160 2704 865 298 196 115 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4740 5460 7001 882 Size: 617 3241 720 1541 2219 Fragments arranged by size: 3241 2219 1541 720 617 BsaAI yAC'GTr Cuts at: 493 1204 2505 3814 4651 6129 493 Size: 711 1301 1309 837 1478 2702 Fragments arranged by size: 2702 1478 1309 1301 837 711 BsaBI GATnn'nnATC Cuts at: 4583 8332 4583 Size: 3749 4589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2751 4521 5196 6145 Size: 53 83 186 936 1218 1770 675 949 Cuts at: 6145 6527 7162 275 Size: 382 635 1451 Fragments arranged by size: 1770 1451 1218 949 936 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 4575 4920 5211 6771 7169 7443 7590 8001 Size: 2088 345 291 1560 398 274 147 411 Cuts at: 8001 2487 Size: 2824 Fragments arranged by size: 2824 2088 1560 411 398 345 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 2457 2468 2487 Size: 30 165 30 1020 30 4 11 19 Cuts at: 2487 2498 4617 4647 5150 5180 1178 Size: 11 2119 30 503 30 4336 Fragments arranged by size: 4336 2119 1020 503 165 30 30 30 30 30 19 11 11 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3368 3641 4130 4520 5300 1186 Size: 2182 273 489 390 780 4224 Fragments arranged by size: 4224 2182 780 489 390 273 BseYI C'CCAG_C Cuts at: 2937 4667 5014 5019 5440 5633 7541 8037 2937 Size: 1730 347 5 421 193 1908 496 3238 Fragments arranged by size: 3238 1908 1730 496 421 347 193 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4427 5523 1163 Size: 573 2691 1096 3978 Fragments arranged by size: 3978 2691 1096 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5118 5328 5788 6549 7577 1540 Size: 275 186 3117 210 460 761 1028 2301 Fragments arranged by size: 3117 2301 1028 761 460 275 210 186 BsiHKAI G_wGCw'C Cuts at: 2094 3288 6399 6484 7555 2094 Size: 1194 3111 85 1071 2877 Fragments arranged by size: 3111 2877 1194 1071 85 BsiWI C'GTAC_G Cuts at: 5329 5329 Size: 8338 BsmI GAATG_Cn' Cuts at: 4694 5849 5942 4694 Size: 1155 93 7090 Fragments arranged by size: 7090 1155 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2742 4536 2742 Size: 1794 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2094 2457 2993 3288 4641 5634 Size: 399 298 6 363 536 295 1353 993 Cuts at: 5634 5657 6399 6484 7555 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1353 1071 993 742 536 399 363 298 295 85 23 6 BspEI T'CCGG_A Cuts at: 4575 8001 4575 Size: 3426 4912 BspHI T'CATG_A Cuts at: 2764 3826 6227 7957 2764 Size: 1062 2401 1730 3145 Fragments arranged by size: 3145 2401 1730 1062 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3740 4149 4488 4502 5209 5693 844 Size: 2896 409 339 14 707 484 3489 Fragments arranged by size: 3489 2896 707 484 409 339 14 BsrBI CCG'CTC Cuts at: 1394 1823 3669 6225 1394 Size: 429 1846 2556 3507 Fragments arranged by size: 3507 2556 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6833 7007 65 Size: 1183 5585 174 1396 Fragments arranged by size: 5585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4144 4465 4920 6982 7169 1700 Size: 110 2334 321 455 2062 187 2869 Fragments arranged by size: 2869 2334 2062 455 321 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8338 BssHII G'CGCG_C Cuts at: 1925 3371 1925 Size: 1446 6892 BssSI C'ACGA_G Cuts at: 4427 4445 4928 6398 7410 4427 Size: 18 483 1470 1012 5355 Fragments arranged by size: 5355 1470 1012 483 18 BstAPI GCAn_nnn'nTGC Cuts at: 794 5551 794 Size: 4757 3581 BstBI TT'CG_AA Cuts at: 7142 7142 Size: 8338 BstEII G'GTnAC_C Cuts at: 1184 5002 1184 Size: 3818 4520 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3387 2029 Size: 1358 6980 BstYI r'GATC_y Cuts at: 1972 2490 3694 5668 6035 6420 6437 7889 7975 Size: 518 1204 1974 367 385 17 1452 86 Cuts at: 7975 7987 8333 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1974 1452 1204 518 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2329 2871 2994 4473 4788 5059 513 Size: 552 1264 542 123 1479 315 271 3792 Fragments arranged by size: 3792 1479 1264 552 542 315 271 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7599 Fragments arranged by size: 7599 683 56 BtsI GCAGTG_nn' Cuts at: 2685 4057 5540 5854 6648 6668 2685 Size: 1372 1483 314 794 20 4355 Fragments arranged by size: 4355 1483 1372 794 314 20 ClaI AT'CG_AT Cuts at: 1554 5362 1554 Size: 3808 4530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4033 4068 529 Size: 35 3469 35 4799 Fragments arranged by size: 4799 3469 35 35 DraI TTT'AAA Cuts at: 3768 5642 3768 Size: 1874 6464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5195 5332 7345 809 Size: 507 3879 137 2013 1802 Fragments arranged by size: 3879 2013 1802 507 137 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3271 3723 4467 Size: 54 1425 50 275 18 1441 452 744 Cuts at: 4467 4726 5115 5785 6674 8303 8 Size: 259 389 670 889 1629 43 Fragments arranged by size: 1629 1441 1425 889 744 670 452 389 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5115 5785 1537 Size: 275 3303 670 4090 Fragments arranged by size: 4090 3303 670 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 6268 1353 Size: 1382 3533 3423 Fragments arranged by size: 3533 3423 1382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2066 6925 7299 7445 387 Size: 1064 138 477 4859 374 146 1280 Fragments arranged by size: 4859 1280 1064 477 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 3286 2092 Size: 1194 7144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2102 2170 2450 2734 2819 3111 Size: 393 430 40 68 280 284 85 292 Cuts at: 3111 3207 3728 5152 6401 6998 7785 8053 1239 Size: 96 521 1424 1249 597 787 268 1524 Fragments arranged by size: 1524 1424 1249 787 597 521 430 393 292 284 280 268 96 85 68 40 EcoNI CCTnn'n_nnAGG Cuts at: 2387 5037 2387 Size: 2650 5688 EcoO109I rG'GnC_Cy Cuts at: 3712 4138 4933 5131 3712 Size: 426 795 198 6919 Fragments arranged by size: 6919 795 426 198 EcoRI G'AATT_C Cuts at: 3028 5235 5647 6025 3028 Size: 2207 412 378 5341 Fragments arranged by size: 5341 2207 412 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5059 5059 Size: 8338 FspI TGC'GCA Cuts at: 6844 6844 Size: 8338 HaeII r_GCGC'y Cuts at: 1678 2723 7485 1678 Size: 1045 4762 2531 Fragments arranged by size: 4762 2531 1045 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3017 3049 3780 3812 4539 Size: 32 135 32 1445 32 731 32 727 Cuts at: 4539 4571 6979 7011 7053 7085 1373 Size: 32 2408 32 42 32 2626 Fragments arranged by size: 2626 2408 1445 731 727 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 5682 5928 669 Size: 988 281 1479 2265 246 3079 Fragments arranged by size: 3079 2265 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 3395 5424 748 Size: 2647 2029 3662 Fragments arranged by size: 3662 2647 2029 HpaI GTT'AAC Cuts at: 3417 5928 3417 Size: 2511 5827 KpnI G_GTAC'C Cuts at: 5663 5663 Size: 8338 MfeI C'AATT_G Cuts at: 5937 5937 Size: 8338 MluI A'CGCG_T Cuts at: 8214 8214 Size: 8338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2203 3072 4367 4434 4695 5254 5409 7452 Size: 898 869 1295 67 261 559 155 2043 Cuts at: 7452 7636 1305 Size: 184 2007 Fragments arranged by size: 2043 2007 1295 898 869 559 261 184 155 67 MscI TGG'CCA Cuts at: 10 64 1489 1832 4728 10 Size: 54 1425 343 2896 3620 Fragments arranged by size: 3620 2896 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2781 3963 4649 5000 6296 6655 Size: 648 1359 256 1182 686 351 1296 359 Cuts at: 6655 6814 518 Size: 159 2042 Fragments arranged by size: 2042 1359 1296 1182 686 648 359 351 256 159 MspA1I CmG'CkG Cuts at: 1606 2080 2922 3276 5754 6431 7579 7824 1606 Size: 474 842 354 2478 677 1148 245 2120 Fragments arranged by size: 2478 2120 1148 842 677 474 354 245 NaeI GCC'GGC Cuts at: 4467 4467 Size: 8338 NcoI C'CATG_G Cuts at: 513 1065 2329 2871 4473 4788 5059 513 Size: 552 1264 542 1602 315 271 3792 Fragments arranged by size: 3792 1602 1264 552 542 315 271 NdeI CA'TA_TG Cuts at: 387 387 Size: 8338 NgoMIV G'CCGG_C Cuts at: 4465 4465 Size: 8338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8338 NotI GC'GGCC_GC Cuts at: 5785 5785 Size: 8338 NspI r_CATG'y Cuts at: 1132 1229 3586 3794 4596 5696 7241 1132 Size: 97 2357 208 802 1100 1545 2229 Fragments arranged by size: 2357 2229 1545 1100 802 208 97 PasI CC'CwG_GG Cuts at: 1348 3716 1348 Size: 2368 5970 PciI A'CATG_T Cuts at: 3582 7237 3582 Size: 3655 4683 PflMI CCAn_nnn'nTGG Cuts at: 1108 2971 1108 Size: 1863 6475 PmeI GTTT'AAAC Cuts at: 5642 5642 Size: 8338 PmlI CAC'GTG Cuts at: 2505 4651 2505 Size: 2146 6192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6407 6439 7920 7952 6407 Size: 32 1481 32 6793 Fragments arranged by size: 6793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3712 4138 5131 3712 Size: 426 993 6919 Fragments arranged by size: 6919 993 426 PshAI GACnn'nnGTC Cuts at: 1613 4287 1613 Size: 2674 5664 PsiI TTA'TAA Cuts at: 5908 5908 Size: 8338 PspXI vC'TCGA_Gb Cuts at: 4976 4976 Size: 8338 PstI C_TGCA'G Cuts at: 830 2150 4168 4499 5547 5690 830 Size: 1320 2018 331 1048 143 3478 Fragments arranged by size: 3478 2018 1320 1048 331 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8338 PvuII CAG'CTG Cuts at: 2080 2922 2080 Size: 842 7496 SacI G_AGCT'C Cuts at: 2094 3288 2094 Size: 1194 7144 SalI G'TCGA_C Cuts at: 1936 5680 1936 Size: 3744 4594 SbfI CC_TGCA'GG Cuts at: 4499 5690 4499 Size: 1191 7147 ScaI AGT'ACT Cuts at: 6586 8298 6586 Size: 1712 6626 SfcI C'TryA_G Cuts at: 826 1045 2146 3432 4164 4460 4495 5543 5686 Size: 219 1101 1286 732 296 35 1048 143 Cuts at: 5686 6821 7502 7693 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1286 1135 1101 1048 732 681 296 219 191 143 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8338 SmaI CCC'GGG Cuts at: 1872 5665 1872 Size: 3793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3463 3623 3679 3774 4976 6442 Size: 197 926 1520 160 56 95 1202 1466 Cuts at: 6442 7343 7605 820 Size: 901 262 1553 Fragments arranged by size: 1553 1520 1466 1202 926 901 262 197 160 95 56 SnaBI TAC'GTA Cuts at: 493 6129 493 Size: 5636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8338 SphI G_CATG'C Cuts at: 1132 5696 1132 Size: 4564 3774 SspI AAT'ATT Cuts at: 5 52 3046 6262 5 Size: 47 2994 3216 2081 Fragments arranged by size: 3216 2994 2081 47 StuI AGG'CCT Cuts at: 1517 3736 1517 Size: 2219 6119 StyI C'CwwG_G Cuts at: 513 1065 2060 2329 2619 2871 4473 4788 5059 Size: 552 995 269 290 252 1602 315 271 Cuts at: 5059 5777 513 Size: 718 3074 Fragments arranged by size: 3074 1602 995 718 552 315 290 271 269 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2461 6365 6382 6535 6720 7165 325 Size: 1255 881 3904 17 153 185 445 1498 Fragments arranged by size: 3904 1498 1255 881 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3780 6584 8296 Size: 274 80 33 51 1443 1803 2804 1712 Cuts at: 8296 96 Size: 138 Fragments arranged by size: 2804 1803 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2217 2249 2423 2455 2457 2489 4261 4293 4320 Size: 32 174 32 2 32 1772 32 27 Cuts at: 4320 4352 5150 5182 8298 8330 2217 Size: 32 798 32 3116 32 2225 Fragments arranged by size: 3116 2225 1772 798 174 32 32 32 32 32 32 32 27 2 Tth111I GACn'n_nGTC Cuts at: 1316 2558 2669 3930 4523 1316 Size: 1242 111 1261 593 5131 Fragments arranged by size: 5131 1261 1242 593 111 XbaI T'CTAG_A Cuts at: 5674 5674 Size: 8338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4103 4786 4103 Size: 683 7655 XhoI C'TCGA_G Cuts at: 1943 4976 1943 Size: 3033 5305 XmaI C'CCGG_G Cuts at: 1870 5663 1870 Size: 3793 4545 XmnI GAAnn'nnTTC Cuts at: 5042 6467 5042 Size: 1425 6913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6146 276 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BfrBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI