Restriction map for
pFN21AB9577
(Linear) (Six-base) MAPSORT of: KIBB9577.seq Check: 5501 from: 1 to: 3633
KIBB9577.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1735 2497 3204 3633
Size: 1735 762 707 429
Fragments arranged by size:
1735 762 707 429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 3633
Size: 814 2819
AflII C'TTAA_G
Cuts at: 0 1674 3633
Size: 1674 1959
AflIII A'CryG_T
Cuts at: 0 1577 1944 2645 2801 3633
Size: 1577 367 701 156 832
Fragments arranged by size:
1577 832 701 367 156
AgeI A'CCGG_T
Cuts at: 0 2915 3633
Size: 2915 718
AleI CACnn'nnGTG
Cuts at: 0 520 1958 2644 2995 3633
Size: 520 1438 686 351 638
Fragments arranged by size:
1438 686 638 520 351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1934 1966 2902 2934 3633
Size: 1934 32 936 32 699
Fragments arranged by size:
1934 936 699 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1195 3129 3546 3633
Size: 1195 1934 417 87
Fragments arranged by size:
1934 1195 417 87
ApoI r'AATT_y
Cuts at: 0 1023 1855 1894 2186 3230 3633
Size: 1023 832 39 292 1044 403
Fragments arranged by size:
1044 1023 832 403 292 39
AseI AT'TA_AT
Cuts at: 0 1043 1847 3633
Size: 1043 804 1786
Fragments arranged by size:
1786 1043 804
AvaI C'yCGr_G
Cuts at: 0 82 350 2971 3633
Size: 82 268 2621 662
Fragments arranged by size:
2621 662 268 82
AvrII C'CTAG_G
Cuts at: 0 55 3633
Size: 55 3578
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2821 2854 3283 3316 3633
Size: 2821 33 429 33 317
Fragments arranged by size:
2821 429 317 33 33
BamHI G'GATC_C
Cuts at: 0 485 3633
Size: 485 3148
BanI G'GyrC_C
Cuts at: 0 2148 3591 3633
Size: 2148 1443 42
Fragments arranged by size:
2148 1443 42
BanII G_rGCy'C
Cuts at: 0 83 89 452 1283 3629 3633
Size: 83 6 363 831 2346 4
Fragments arranged by size:
2346 831 363 83 6 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 314 2552 3633
Size: 314 2238 1081
Fragments arranged by size:
2238 1081 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2962 2996 3633
Size: 2962 34 637
Fragments arranged by size:
2962 637 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1206 2949 3633
Size: 1206 1743 684
Fragments arranged by size:
1743 1206 684
BlpI GC'TnA_GC
Cuts at: 0 4 2096 3633
Size: 4 2092 1537
Fragments arranged by size:
2092 1537 4
Bme1580I G_kGCm'C
Cuts at: 0 988 2636 3633
Size: 988 1648 997
Fragments arranged by size:
1648 997 988
BmgBI CAC'GTC
Cuts at: 0 663 1947 3633
Size: 663 1284 1686
Fragments arranged by size:
1686 1284 663
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 2221 2755 3580 3633
Size: 474 1747 534 825 53
Fragments arranged by size:
1747 825 534 474 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1751 3633
Size: 1751 1882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 97 165 445 729 1106 1202 1723
Size: 57 40 68 280 284 377 96 521
Cuts at: 1723 3147 3633
Size: 1424 486
Fragments arranged by size:
1424 521 486 377 284 280 96 68
57 40
Bpu10I CC'TnA_GC
Cuts at: 0 1196 2598 3633
Size: 1196 1402 1035
Fragments arranged by size:
1402 1196 1035
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1443 1639 1754 3633
Size: 1443 196 115 1879
Fragments arranged by size:
1879 1443 196 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 2735 3455 3633
Size: 2735 720 178
Fragments arranged by size:
2735 720 178
BsaAI yAC'GTr
Cuts at: 0 500 1809 2646 3633
Size: 500 1309 837 987
Fragments arranged by size:
1309 987 837 500
BsaBI GATnn'nnATC
Cuts at: 0 2578 3633
Size: 2578 1055
BsaHI Gr'CG_yC
Cuts at: 0 746 2516 3191 3633
Size: 746 1770 675 442
Fragments arranged by size:
1770 746 675 442
BsaWI w'CCGG_w
Cuts at: 0 482 2570 2915 3206 3633
Size: 482 2088 345 291 427
Fragments arranged by size:
2088 482 427 345 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 452 463 482 493 2612 2642
Size: 418 30 4 11 19 11 2119 30
Cuts at: 2642 3145 3175 3633
Size: 503 30 458
Fragments arranged by size:
2119 503 458 418 30 30 30 19
11 11 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1363 1636 2125 2515 3295 3633
Size: 1363 273 489 390 780 338
Fragments arranged by size:
1363 780 489 390 338 273
BseYI C'CCAG_C
Cuts at: 0 932 2662 3009 3014 3435 3628 3633
Size: 932 1730 347 5 421 193 5
Fragments arranged by size:
1730 932 421 347 193 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2422 3518 3633
Size: 2422 1096 115
Fragments arranged by size:
2422 1096 115
BsiEI CG_ry'CG
Cuts at: 0 3113 3323 3633
Size: 3113 210 310
Fragments arranged by size:
3113 310 210
BsiHKAI G_wGCw'C
Cuts at: 0 89 1283 3633
Size: 89 1194 2350
Fragments arranged by size:
2350 1194 89
BsiWI C'GTAC_G
Cuts at: 0 3324 3633
Size: 3324 309
BsmI GAATG_Cn'
Cuts at: 0 2689 3633
Size: 2689 944
BsmBI CGTCTCn'nnnn_
Cuts at: 0 737 2531 3633
Size: 737 1794 1102
Fragments arranged by size:
1794 1102 737
Bsp1286I G_dGCh'C
Cuts at: 0 83 89 452 988 1283 2636 3629 3633
Size: 83 6 363 536 295 1353 993 4
Fragments arranged by size:
1353 993 536 363 295 83 6 4
BspEI T'CCGG_A
Cuts at: 0 2570 3633
Size: 2570 1063
BspHI T'CATG_A
Cuts at: 0 759 1821 3633
Size: 759 1062 1812
Fragments arranged by size:
1812 1062 759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1735 2144 2483 2497 3204 3633
Size: 1735 409 339 14 707 429
Fragments arranged by size:
1735 707 429 409 339 14
BsrBI CCG'CTC
Cuts at: 0 1664 3633
Size: 1664 1969
BsrFI r'CCGG_y
Cuts at: 0 2139 2460 2915 3633
Size: 2139 321 455 718
Fragments arranged by size:
2139 718 455 321
BssHII G'CGCG_C
Cuts at: 0 1366 3633
Size: 1366 2267
BssSI C'ACGA_G
Cuts at: 0 2422 2440 2923 3633
Size: 2422 18 483 710
Fragments arranged by size:
2422 710 483 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3546 3633
Size: 3546 87
BstEII G'GTnAC_C
Cuts at: 0 2997 3633
Size: 2997 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 1382 3633
Size: 24 1358 2251
Fragments arranged by size:
2251 1358 24
BstYI r'GATC_y
Cuts at: 0 485 1689 3633
Size: 485 1204 1944
Fragments arranged by size:
1944 1204 485
BtgI C'CryG_G
Cuts at: 0 324 866 989 2468 2783 3054 3633
Size: 324 542 123 1479 315 271 579
Fragments arranged by size:
1479 579 542 324 315 271 123
BtsI GCAGTG_nn'
Cuts at: 0 680 2052 3535 3633
Size: 680 1372 1483 98
Fragments arranged by size:
1483 1372 680 98
ClaI AT'CG_AT
Cuts at: 0 3357 3633
Size: 3357 276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2028 2063 3633
Size: 2028 35 1570
Fragments arranged by size:
2028 1570 35
DraI TTT'AAA
Cuts at: 0 1763 3633
Size: 1763 1870
DrdI GACnn_nn'nnGTC
Cuts at: 0 3190 3327 3633
Size: 3190 137 306
Fragments arranged by size:
3190 306 137
EaeI y'GGCC_r
Cuts at: 0 1266 1718 2462 2721 3110 3633
Size: 1266 452 744 259 389 523
Fragments arranged by size:
1266 744 523 452 389 259
EagI C'GGCC_G
Cuts at: 0 3110 3633
Size: 3110 523
EarI CTCTTCn'nnn_
Cuts at: 0 730 3633
Size: 730 2903
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 61 3633
Size: 61 3572
EcoICRI GAG'CTC
Cuts at: 0 87 1281 3633
Size: 87 1194 2352
Fragments arranged by size:
2352 1194 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 97 165 445 729 814 1106 1202
Size: 57 40 68 280 284 85 292 96
Cuts at: 1202 1723 3147 3633
Size: 521 1424 486
Fragments arranged by size:
1424 521 486 292 284 280 96 85
68 57 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 382 3032 3633
Size: 382 2650 601
Fragments arranged by size:
2650 601 382
EcoO109I rG'GnC_Cy
Cuts at: 0 1707 2133 2928 3126 3633
Size: 1707 426 795 198 507
Fragments arranged by size:
1707 795 507 426 198
EcoRI G'AATT_C
Cuts at: 0 1023 3230 3633
Size: 1023 2207 403
Fragments arranged by size:
2207 1023 403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3054 3633
Size: 3054 579
HaeII r_GCGC'y
Cuts at: 0 718 3633
Size: 718 2915
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1775 1807 2534 2566 3633
Size: 1012 32 731 32 727 32 1067
Fragments arranged by size:
1067 1012 731 727 32 32 32
HincII GTy'rAC
Cuts at: 0 1412 3633
Size: 1412 2221
HindIII A'AGCT_T
Cuts at: 0 1390 3419 3633
Size: 1390 2029 214
Fragments arranged by size:
2029 1390 214
HpaI GTT'AAC
Cuts at: 0 1412 3633
Size: 1412 2221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1067 2362 2429 2690 3249 3404 3633
Size: 198 869 1295 67 261 559 155 229
Fragments arranged by size:
1295 869 559 261 229 198 155 67
MscI TGG'CCA
Cuts at: 0 2723 3633
Size: 2723 910
MslI CAynn'nnrTG
Cuts at: 0 520 776 1958 2644 2995 3633
Size: 520 256 1182 686 351 638
Fragments arranged by size:
1182 686 638 520 351 256
MspA1I CmG'CkG
Cuts at: 0 75 917 1271 3633
Size: 75 842 354 2362
Fragments arranged by size:
2362 842 354 75
NaeI GCC'GGC
Cuts at: 0 2462 3633
Size: 2462 1171
NcoI C'CATG_G
Cuts at: 0 324 866 2468 2783 3054 3633
Size: 324 542 1602 315 271 579
Fragments arranged by size:
1602 579 542 324 315 271
NgoMIV G'CCGG_C
Cuts at: 0 2460 3633
Size: 2460 1173
NspI r_CATG'y
Cuts at: 0 1581 1789 2591 3633
Size: 1581 208 802 1042
Fragments arranged by size:
1581 1042 802 208
PasI CC'CwG_GG
Cuts at: 0 1711 3633
Size: 1711 1922
PciI A'CATG_T
Cuts at: 0 1577 3633
Size: 1577 2056
PflMI CCAn_nnn'nTGG
Cuts at: 0 966 3633
Size: 966 2667
PmlI CAC'GTG
Cuts at: 0 500 2646 3633
Size: 500 2146 987
Fragments arranged by size:
2146 987 500
PpuMI rG'GwC_Cy
Cuts at: 0 1707 2133 3126 3633
Size: 1707 426 993 507
Fragments arranged by size:
1707 993 507 426
PshAI GACnn'nnGTC
Cuts at: 0 2282 3633
Size: 2282 1351
PspXI vC'TCGA_Gb
Cuts at: 0 2971 3633
Size: 2971 662
PstI C_TGCA'G
Cuts at: 0 145 2163 2494 3542 3633
Size: 145 2018 331 1048 91
Fragments arranged by size:
2018 1048 331 145 91
PvuII CAG'CTG
Cuts at: 0 75 917 3633
Size: 75 842 2716
Fragments arranged by size:
2716 842 75
SacI G_AGCT'C
Cuts at: 0 89 1283 3633
Size: 89 1194 2350
Fragments arranged by size:
2350 1194 89
SbfI CC_TGCA'GG
Cuts at: 0 2494 3633
Size: 2494 1139
SfcI C'TryA_G
Cuts at: 0 141 1427 2159 2455 2490 3538 3633
Size: 141 1286 732 296 35 1048 95
Fragments arranged by size:
1286 1048 732 296 141 95 35
SmlI C'TyrA_G
Cuts at: 0 1458 1618 1674 1769 2971 3633
Size: 1458 160 56 95 1202 662
Fragments arranged by size:
1458 1202 662 160 95 56
SspI AAT'ATT
Cuts at: 0 1041 3633
Size: 1041 2592
StuI AGG'CCT
Cuts at: 0 1731 3633
Size: 1731 1902
StyI C'CwwG_G
Cuts at: 0 55 324 614 866 2468 2783 3054 3633
Size: 55 269 290 252 1602 315 271 579
Fragments arranged by size:
1602 579 315 290 271 269 252 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 456 3633
Size: 456 3177
TatI w'GTAC_w
Cuts at: 0 1775 3633
Size: 1775 1858
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 212 244 418 450 452 484 2256 2288
Size: 212 32 174 32 2 32 1772 32
Cuts at: 2288 2315 2347 3145 3177 3633
Size: 27 32 798 32 456
Fragments arranged by size:
1772 798 456 212 174 32 32 32
32 32 32 27 2
Tth111I GACn'n_nGTC
Cuts at: 0 553 664 1925 2518 3633
Size: 553 111 1261 593 1115
Fragments arranged by size:
1261 1115 593 553 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2098 2781 3633
Size: 2098 683 852
Fragments arranged by size:
2098 852 683
XhoI C'TCGA_G
Cuts at: 0 2971 3633
Size: 2971 662
XmnI GAAnn'nnTTC
Cuts at: 0 3037 3633
Size: 3037 596
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AleI AloI AlwNI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PciI PflMI PmlI PpuMI PshAI
PspXI PstI PvuII SacI SbfI SfcI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI AlfI ApaI
ApaLI AscI AsiSI BbeI BbvCI BciVI BclI BfrBI
BglII BmtI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI
DraIII EcoRV FseI FspI FspAI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PfoI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
XbaI XmaI ZraI