(Circular) (Six-base) MAPSORT of: pFN21AB9575.seq Check: 5494 from: 1 to: 6526 pFN21AB9575 6526 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2838 2871 3869 1937 Size: 901 33 998 4594 Fragments arranged by size: 4594 998 901 33 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2499 2932 3485 3545 3824 4589 5973 6241 Size: 867 433 553 60 279 765 1384 268 Cuts at: 6241 1632 Size: 1917 Fragments arranged by size: 1917 1384 867 765 553 433 279 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 5425 6402 1203 Size: 4222 977 1327 Fragments arranged by size: 4222 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 5255 5255 Size: 6526 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2250 2571 3252 1184 Size: 1066 321 681 4458 Fragments arranged by size: 4458 1066 681 321 AlwNI CAG_nnn'CTG Cuts at: 3463 4269 5841 3463 Size: 806 1572 4148 Fragments arranged by size: 4148 1572 806 ApaLI G'TGCA_C Cuts at: 3425 4583 5739 3425 Size: 1158 1156 4212 Fragments arranged by size: 4212 1158 1156 ApoI r'AATT_y Cuts at: 1493 3668 3779 3835 4061 4213 6322 1493 Size: 2175 111 56 226 152 2109 1697 Fragments arranged by size: 2175 2109 1697 226 152 111 56 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2471 3851 1870 Size: 73 104 424 1380 4545 Fragments arranged by size: 4545 1380 424 104 73 BamHI G'GATC_C Cuts at: 3594 3856 4223 3594 Size: 262 367 5897 Fragments arranged by size: 5897 367 262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3847 5302 618 Size: 325 217 627 2060 1455 1842 Fragments arranged by size: 2060 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3450 3845 1391 Size: 2059 395 4072 Fragments arranged by size: 4072 2059 395 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2506 3130 3601 928 Size: 971 607 624 471 3853 Fragments arranged by size: 3853 971 624 607 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2633 2667 4717 4751 1903 Size: 34 696 34 2050 34 3678 Fragments arranged by size: 3678 2050 696 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 2286 1548 Size: 738 5788 BfrBI ATG'CAT Cuts at: 3235 3235 Size: 6526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3557 5137 136 Size: 107 122 71 631 605 1885 1580 1525 Fragments arranged by size: 1885 1580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 3943 3943 Size: 6526 Bme1580I G_kGCm'C Cuts at: 1790 3411 3429 4587 5743 1790 Size: 1621 18 1158 1156 2573 Fragments arranged by size: 2573 1621 1158 1156 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4257 5215 442 Size: 416 53 83 399 24 2840 958 1753 Fragments arranged by size: 2840 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 3035 3281 5186 1239 Size: 1117 679 246 1905 2579 Fragments arranged by size: 2579 1905 1117 679 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4651 5516 5814 4651 Size: 865 298 5363 Fragments arranged by size: 5363 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3814 5189 882 Size: 617 2315 1375 2219 Fragments arranged by size: 2315 2219 1375 617 BsaAI yAC'GTr Cuts at: 493 1204 3195 4317 493 Size: 711 1991 1122 2702 Fragments arranged by size: 2702 1991 1122 711 BsaBI GATnn'nnATC Cuts at: 3719 6520 3719 Size: 2801 3725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4333 4715 5350 275 Size: 53 83 186 936 2800 382 635 1451 Fragments arranged by size: 2800 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2030 3591 4959 5357 5631 5778 6189 2030 Size: 1561 1368 398 274 147 411 2367 Fragments arranged by size: 2367 1561 1368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 1178 Size: 30 165 30 1173 30 5098 Fragments arranged by size: 5098 1173 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 2777 3143 3800 1186 Size: 1588 3 366 657 3912 Fragments arranged by size: 3912 1588 657 366 3 BseYI C'CCAG_C Cuts at: 2192 2903 3498 5729 6225 2192 Size: 711 595 2231 496 2493 Fragments arranged by size: 2493 2231 711 595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2957 1163 Size: 573 1221 4732 Fragments arranged by size: 4732 1221 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3976 4737 5765 1540 Size: 275 186 1975 761 1028 2301 Fragments arranged by size: 2301 1975 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3429 4587 4672 5743 2118 Size: 1311 1158 85 1071 2901 Fragments arranged by size: 2901 1311 1158 1071 85 BsmI GAATG_Cn' Cuts at: 3288 4037 4130 3288 Size: 749 93 5684 Fragments arranged by size: 5684 749 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 3411 3429 3450 3845 4587 4672 Size: 399 328 1293 18 21 395 742 85 Cuts at: 4672 5743 1391 Size: 1071 2174 Fragments arranged by size: 2174 1293 1071 742 399 395 328 85 21 18 BspEI T'CCGG_A Cuts at: 3591 6189 3591 Size: 2598 3928 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3881 844 Size: 3037 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2092 4413 1394 Size: 429 269 2321 3507 Fragments arranged by size: 3507 2321 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 2484 2509 3249 5021 5195 65 Size: 1183 1236 25 740 1772 174 1396 Fragments arranged by size: 1772 1396 1236 1183 740 174 25 BsrFI r'CCGG_y Cuts at: 1700 1810 5170 5357 1700 Size: 110 3360 187 2869 Fragments arranged by size: 3360 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 3632 4586 5598 3632 Size: 954 1012 4560 Fragments arranged by size: 4560 1012 954 BstAPI GCAn_nnn'nTGC Cuts at: 794 3241 794 Size: 2447 4079 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 2885 3070 3344 1184 Size: 1701 185 274 4366 Fragments arranged by size: 4366 1701 274 185 BstXI CCAn_nnnn'nTGG Cuts at: 3547 3547 Size: 6526 BstYI r'GATC_y Cuts at: 1972 2218 2403 3594 3771 3856 4223 4608 4625 Size: 246 185 1191 177 85 367 385 17 Cuts at: 4625 6077 6163 6175 6521 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 246 185 177 86 85 17 12 BstZ17I GTA'TAC Cuts at: 2872 2872 Size: 6526 Bsu36I CC'TnA_GG Cuts at: 2071 2280 2071 Size: 209 6317 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5035 Fragments arranged by size: 5035 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3706 487 Size: 683 56 2480 3307 Fragments arranged by size: 3307 2480 683 56 BtsI GCAGTG_nn' Cuts at: 4042 4836 4856 4042 Size: 794 20 5712 Fragments arranged by size: 5712 794 20 ClaI AT'CG_AT Cuts at: 1554 2694 1554 Size: 1140 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2548 2583 529 Size: 35 1984 35 4472 Fragments arranged by size: 4472 1984 35 35 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3353 3734 5533 809 Size: 507 2037 381 1799 1802 Fragments arranged by size: 2037 1802 1799 507 381 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3246 3549 Size: 54 1425 50 275 18 177 1239 303 Cuts at: 3549 3973 4862 6491 8 Size: 424 889 1629 43 Fragments arranged by size: 1629 1425 1239 889 424 303 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 3032 4456 1353 Size: 1274 405 1424 3423 Fragments arranged by size: 3423 1424 1274 405 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2957 5113 5487 5633 387 Size: 1064 138 1368 2156 374 146 1280 Fragments arranged by size: 2156 1368 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2499 2932 3035 3281 3485 3545 Size: 393 724 143 433 103 246 204 60 Cuts at: 3545 3824 4589 5186 5973 6241 1239 Size: 279 765 597 787 268 1524 Fragments arranged by size: 1524 787 765 724 597 433 393 279 268 246 204 143 103 60 EcoNI CCTnn'n_nnAGG Cuts at: 2021 2021 Size: 6526 EcoO109I rG'GnC_Cy Cuts at: 2230 2573 2230 Size: 343 6183 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 FspI TGC'GCA Cuts at: 3332 5032 3332 Size: 1700 4826 HaeII r_GCGC'y Cuts at: 1678 5673 1678 Size: 3995 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 3097 3129 5167 Size: 32 135 32 572 32 921 32 2038 Cuts at: 5167 5199 5241 5273 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2038 921 572 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3691 3870 4116 669 Size: 988 281 1753 179 246 3079 Fragments arranged by size: 3079 1753 988 281 246 179 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 2871 5640 5824 1305 Size: 1539 27 2769 184 2007 Fragments arranged by size: 2769 2007 1539 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3248 3551 10 Size: 54 1425 343 177 1239 303 2985 Fragments arranged by size: 2985 1425 1239 343 303 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 3008 3539 3545 4484 4843 5002 Size: 648 1387 455 531 6 939 359 159 Cuts at: 5002 518 Size: 2042 Fragments arranged by size: 2042 1387 939 648 531 455 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2147 2427 2736 3301 3463 3645 3942 4619 Size: 541 280 309 565 162 182 297 677 Cuts at: 4619 5767 6012 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 541 309 297 280 245 182 162 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5035 Fragments arranged by size: 5035 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NsiI A_TGCA'T Cuts at: 3237 3237 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 3181 3239 3337 3884 5429 1132 Size: 97 1952 58 98 547 1545 2229 Fragments arranged by size: 2229 1952 1545 547 98 97 58 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6526 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2498 2676 2796 1108 Size: 1390 178 120 4838 Fragments arranged by size: 4838 1390 178 120 PfoI T'CCnGG_A Cuts at: 2406 3567 2406 Size: 1161 5365 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 4595 4627 6108 6140 3572 Size: 32 991 32 1481 32 3958 Fragments arranged by size: 3958 1481 991 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2573 2230 Size: 343 6183 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PstI C_TGCA'G Cuts at: 830 3459 3878 830 Size: 2629 419 3478 Fragments arranged by size: 3478 2629 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2427 3301 3463 2427 Size: 874 162 5490 Fragments arranged by size: 5490 874 162 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2354 2542 3402 3455 3874 5009 5690 Size: 219 1309 188 860 53 419 1135 681 Cuts at: 5690 5881 826 Size: 191 1471 Fragments arranged by size: 1471 1309 1135 860 681 419 219 191 188 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 3853 1872 Size: 1981 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4630 5531 5793 820 Size: 197 926 2687 901 262 1553 Fragments arranged by size: 2687 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3239 3337 3884 1132 Size: 2107 98 547 3774 Fragments arranged by size: 3774 2107 547 98 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 2016 1517 Size: 499 6027 StyI C'CwwG_G Cuts at: 513 1065 2004 2465 2580 3732 3965 513 Size: 552 939 461 115 1152 233 3074 Fragments arranged by size: 3074 1152 939 552 461 233 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4553 4570 4723 4908 5353 325 Size: 1255 2973 17 153 185 445 1498 Fragments arranged by size: 2973 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3484 4772 6484 Size: 274 80 33 51 1443 1507 1288 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 1712 1507 1443 1288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2203 2235 6486 6518 2203 Size: 32 4251 32 2211 Fragments arranged by size: 4251 2211 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6526 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3010 3544 3010 Size: 534 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 3851 1870 Size: 1981 4545 XmnI GAAnn'nnTTC Cuts at: 2026 4655 2026 Size: 2629 3897 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI