Restriction map for
pFN21AB9571
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BanII BbeI BbrPI BbsI
BbvCI BciVI BcnI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 9 GTATAC GT^[AC][GT]AC
AcsI 782 AAATTC [GA]^AATT[CT]
AfaI 190 GTAC GT^AC
AflII 739 CTTAAG C^TTAAG
AlwNI 464 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 782 AAATTC [GA]^AATT[CT]
AseI 90 ATTAAT AT^TAAT
AsnI 90 ATTAAT AT^TAAT
AspHI 512 GAGCAC G[AT]GC[AT]^C
AspI 167 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 755 CCCGAG C^[CT]CG[GA]G
BamHI 903 GGATCC G^GATCC
BcgI 810 GCATGAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 482 TGATCA T^GATCA
BfaI 766 CTAG C^TAG
BfrI 739 CTTAAG C^TTAAG
BmyI 512 GAGCAC G[AGT]GC[ACT]^C
BsaAI 709 TACGTG [CT]AC^GT[GA]
BseRI 744 GAGGAG GAGGAG(10/8)
BsiHKAI 512 GAGCAC G[AT]GC[AT]^C
BsmAI 816 GTCTC GTCTC(1/5)
BsmI 714 GAATGC GAATGC(1/-1)
BsoBI 755 CCCGAG C^[CT]CG[GA]G
Bsp1286I 512 GAGCAC G[AGT]GC[ACT]^C
BspHI 623 TCATGA T^CATGA
Bst1107I 9 GTATAC GTA^TAC
BstAPI 391 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 9 GTATAC GTA^TAC
DdeI 350 CTCAG C^T[ACGT]AG
DsaI 833 CCATGG C^C[GA][CT]GG
EarI 697 GAAGAG CTCTTC(1/4)
EcoNI 469 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 482 TGATCA T^GATCA
HphI 850 GGTGA GGTGA(8/7)
Ksp632I 697 GAAGAG CTCTTC(1/4)
MaeI 766 CTAG C^TAG
MaeII 710 ACGT A^CGT
NcoI 833 CCATGG C^CATGG
NdeI 895 CATATG CA^TATG
PflFI 167 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 90 ATTAAT AT^TAAT
RcaI 623 TCATGA T^CATGA
RsaI 190 GTAC GT^AC
SapI 697 GAAGAGC GCTCTTC(1/4)
SfaNI 687 GATGC GCATC(5/9)
SfiI 283 GGCCAGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 739 CTTAAG C^T[CT][GA]AG
TaqI 31 TCGA T^CGA
Tth111I 167 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 31 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 116 AGCT AG^CT
759 AGCT
BalI 132 TGGCCA TGG^CCA
342 TGGCCA
BanI 442 GGCACC G^G[CT][GA]CC
821 GGCACC
BglI 284 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
499 GCCAGTTTGGC
BpmI 451 CTCCAG CTGGAG(16/14)
677 CTGGAG
BsrDI 596 GCAATG GCAATG(2/0)
928 GCAATG
EaeI 132 TGGCCA [CT]^GGCC[GA]
342 TGGCCA
EcoT14I 78 CCAAGG C^C[AT][AT]GG
833 CCATGG
MluNI 132 TGGCCA TGG^CCA
342 TGGCCA
MscI 132 TGGCCA TGG^CCA
342 TGGCCA
MslI 823 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
942 CATCCGAGTG
PleI 348 GACTC GAGTC(4/5)
814 GAGTC
PshAI 327 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
411 GACCATCGTC
StyI 78 CCAAGG C^C[AT][AT]GG
833 CCATGG
TfiI 63 GATTC G^A[AT]TC
665 GAATC
Tsp509I 607 AATT ^AATT
783 AATT
XcmI 150 CCAGGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
249 CCAGATCCAGGGTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AlwI AvaII BbvI BsaBI BsaJI
BsgI BsiYI BslI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MamI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tru9I
TseI Tsp45I TspRI XhoII