(Circular) (Six-base) MAPSORT of: pFN21AB9569.seq Check: 8872 from: 1 to: 6928 pFN21AB9569 6928 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4738 278 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4271 1937 Size: 2334 4594 Acc65I G'GTAC_C Cuts at: 4249 4249 Size: 6928 AclI AA'CG_TT Cuts at: 1563 5055 5428 1563 Size: 3492 373 3063 Fragments arranged by size: 3492 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2944 3474 4991 6375 6643 1632 Size: 624 688 530 1517 1384 268 1917 Fragments arranged by size: 1917 1517 1384 688 624 530 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6731 AflIII A'CryG_T Cuts at: 1203 3012 5827 6804 1203 Size: 1809 2815 977 1327 Fragments arranged by size: 2815 1809 1327 977 AgeI A'CCGG_T Cuts at: 5759 5759 Size: 6928 AhdI GACnn_n'nnGTC Cuts at: 5657 5657 Size: 6928 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6928 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2454 2457 1184 Size: 1270 3 5655 Fragments arranged by size: 5655 1270 3 AlwNI CAG_nnn'CTG Cuts at: 2395 3719 3722 3782 4094 4671 6243 2395 Size: 1324 3 60 312 577 1572 3080 Fragments arranged by size: 3080 1572 1324 577 312 60 3 ApaLI G'TGCA_C Cuts at: 4985 6141 4985 Size: 1156 5772 ApoI r'AATT_y Cuts at: 1493 3103 4237 4463 4615 6724 1493 Size: 1610 1134 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 1134 226 152 AseI AT'TA_AT Cuts at: 160 1032 5482 160 Size: 872 4450 1606 Fragments arranged by size: 4450 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 AvaI C'yCGr_G Cuts at: 1870 1943 4253 1870 Size: 73 2310 4545 Fragments arranged by size: 4545 2310 73 BamHI G'GATC_C Cuts at: 4258 4625 4258 Size: 367 6561 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3808 3833 4249 5704 618 Size: 325 217 627 2021 25 416 1455 1842 Fragments arranged by size: 2021 1842 1455 627 416 325 217 25 BanII G_rGCy'C Cuts at: 1391 2844 2931 4000 4247 1391 Size: 1453 87 1069 247 4072 Fragments arranged by size: 4072 1453 1069 247 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 5841 Fragments arranged by size: 5841 971 116 BbvCI CC'TCA_GC Cuts at: 2685 2999 3547 3589 2685 Size: 314 548 42 6024 Fragments arranged by size: 6024 548 314 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5119 5153 1903 Size: 34 3182 34 3678 Fragments arranged by size: 3678 3182 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4820 6030 4820 Size: 1210 5718 BclI T'GATC_A Cuts at: 1548 3238 1548 Size: 1690 5238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4190 4220 5539 Size: 107 122 71 631 605 2518 30 1319 Cuts at: 5539 136 Size: 1525 Fragments arranged by size: 2518 1525 1319 631 605 122 107 71 30 BglII A'GATC_T Cuts at: 2022 6923 2022 Size: 4901 2027 BlpI GC'TnA_GC Cuts at: 4345 4345 Size: 6928 Bme1580I G_kGCm'C Cuts at: 1790 3100 4989 6145 1790 Size: 1310 1889 1156 2573 Fragments arranged by size: 2573 1889 1310 1156 BmgBI CAC'GTC Cuts at: 1318 3558 1318 Size: 2240 4688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2337 2838 3975 Size: 416 53 83 399 24 920 501 1137 Cuts at: 3975 4659 5617 442 Size: 684 958 1753 Fragments arranged by size: 1753 1137 958 920 684 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2312 1056 Size: 1256 5672 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3082 3888 3082 Size: 806 6122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 2351 2466 3070 3093 3522 3849 5588 Size: 1095 17 115 604 23 429 327 1739 Cuts at: 5588 1239 Size: 2579 Fragments arranged by size: 2579 1739 1095 604 429 327 115 23 17 Bpu10I CC'TnA_GC Cuts at: 2685 2999 3547 3589 3863 2685 Size: 314 548 42 274 5750 Fragments arranged by size: 5750 548 314 274 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 2882 3145 5053 5918 6216 2873 Size: 9 263 1908 865 298 3585 Fragments arranged by size: 3585 1908 865 298 263 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2654 3152 3617 4081 5591 882 Size: 617 1155 498 465 464 1510 2219 Fragments arranged by size: 2219 1510 1155 617 498 465 464 BsaAI yAC'GTr Cuts at: 493 1204 4719 493 Size: 711 3515 2702 Fragments arranged by size: 3515 2702 711 BsaBI GATnn'nnATC Cuts at: 2021 6922 2021 Size: 4901 2027 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2342 2630 4735 5117 Size: 53 83 186 936 809 288 2105 382 Cuts at: 5117 5752 275 Size: 635 1451 Fragments arranged by size: 2105 1451 936 809 635 382 288 186 83 53 BsaWI w'CCGG_w Cuts at: 5361 5759 6033 6180 6591 5361 Size: 398 274 147 411 5698 Fragments arranged by size: 5698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3485 3515 3520 3550 3634 Size: 30 165 30 2082 30 5 30 84 Cuts at: 3634 3664 4118 4148 1178 Size: 30 454 30 3958 Fragments arranged by size: 3958 2082 454 165 84 30 30 30 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2121 2852 2870 4008 1186 Size: 935 731 18 1138 4106 Fragments arranged by size: 4106 1138 935 731 18 BseYI C'CCAG_C Cuts at: 2262 2298 2349 2739 2828 3362 3880 6131 6627 Size: 36 51 390 89 534 518 2251 496 Cuts at: 6627 2262 Size: 2563 Fragments arranged by size: 2563 2251 534 518 496 390 89 51 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2429 3432 3723 4092 1163 Size: 573 561 132 1003 291 369 3999 Fragments arranged by size: 3999 1003 573 561 369 291 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4378 5139 6167 1540 Size: 275 186 2377 761 1028 2301 Fragments arranged by size: 2377 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3874 4989 5074 6145 3874 Size: 1115 85 1071 4657 Fragments arranged by size: 4657 1115 1071 85 BsmI GAATG_Cn' Cuts at: 4439 4532 4439 Size: 93 6835 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2844 2931 3100 3874 4000 4247 4989 Size: 399 1054 87 169 774 126 247 742 Cuts at: 4989 5074 6145 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1054 774 742 399 247 169 126 87 85 BspEI T'CCGG_A Cuts at: 6591 6591 Size: 6928 BspHI T'CATG_A Cuts at: 4817 6547 4817 Size: 1730 5198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2571 4283 844 Size: 1727 1712 3489 Fragments arranged by size: 3489 1727 1712 BsrBI CCG'CTC Cuts at: 1394 1823 3867 4815 1394 Size: 429 2044 948 3507 Fragments arranged by size: 3507 2044 948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5423 5597 65 Size: 1183 4175 174 1396 Fragments arranged by size: 4175 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5572 5759 1700 Size: 110 3762 187 2869 Fragments arranged by size: 3762 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2206 96 Size: 2110 4818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6928 BssSI C'ACGA_G Cuts at: 4988 6000 4988 Size: 1012 5916 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 3614 3719 3722 3782 4094 794 Size: 1301 1519 105 3 60 312 3628 Fragments arranged by size: 3628 1519 1301 312 105 60 3 BstBI TT'CG_AA Cuts at: 5732 5732 Size: 6928 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6928 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2168 2074 Size: 94 6834 BstYI r'GATC_y Cuts at: 1972 2022 2487 2521 4258 4625 5010 5027 6479 Size: 50 465 34 1737 367 385 17 1452 Cuts at: 6479 6565 6577 6923 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1737 1452 465 385 367 346 86 50 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 3771 513 Size: 552 2706 3670 Fragments arranged by size: 3670 2706 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6189 Fragments arranged by size: 6189 683 56 BtsI GCAGTG_nn' Cuts at: 2412 3328 3415 4444 5238 5258 2412 Size: 916 87 1029 794 20 4082 Fragments arranged by size: 4082 1029 916 794 87 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6928 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6893 DraI TTT'AAA Cuts at: 2202 4232 2202 Size: 2030 4898 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5935 809 Size: 507 4619 1802 Fragments arranged by size: 4619 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4375 5264 6893 Size: 54 1425 50 275 18 2545 889 1629 Cuts at: 6893 8 Size: 43 Fragments arranged by size: 2545 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4375 1537 Size: 275 2563 4090 Fragments arranged by size: 4090 2563 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3179 3213 3945 4172 4858 1353 Size: 1826 34 732 227 686 3423 Fragments arranged by size: 3423 1826 732 686 227 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2410 2449 5515 5889 6035 387 Size: 1064 138 821 39 3066 374 146 1280 Fragments arranged by size: 3066 1280 1064 821 374 146 138 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2334 2351 2466 2944 3070 3093 Size: 393 624 78 17 115 478 126 23 Cuts at: 3093 3474 3522 3849 4991 5588 6375 6643 1239 Size: 381 48 327 1142 597 787 268 1524 Fragments arranged by size: 1524 1142 787 624 597 478 393 381 327 268 126 115 78 48 23 17 EcoNI CCTnn'n_nnAGG Cuts at: 2103 2103 Size: 6928 EcoO109I rG'GnC_Cy Cuts at: 3858 3858 Size: 6928 EcoRI G'AATT_C Cuts at: 3103 4237 4615 3103 Size: 1134 378 5416 Fragments arranged by size: 5416 1134 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2274 3208 2274 Size: 934 5994 FspI TGC'GCA Cuts at: 5434 5434 Size: 6928 HaeII r_GCGC'y Cuts at: 1678 3536 6075 1678 Size: 1858 2539 2531 Fragments arranged by size: 2539 2531 1858 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2439 2471 3223 Size: 32 135 32 525 32 310 32 752 Cuts at: 3223 3255 3485 3517 4003 4035 5569 5601 5643 Size: 32 230 32 486 32 1534 32 42 Cuts at: 5643 5675 1373 Size: 32 2626 Fragments arranged by size: 2626 1534 752 525 486 310 230 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 4272 4518 669 Size: 988 281 867 1467 246 3079 Fragments arranged by size: 3079 1467 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6928 HpaI GTT'AAC Cuts at: 4518 4518 Size: 6928 KpnI G_GTAC'C Cuts at: 4253 4253 Size: 6928 MfeI C'AATT_G Cuts at: 4527 4527 Size: 6928 MluI A'CGCG_T Cuts at: 6804 6804 Size: 6928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 2612 3528 6042 6226 1305 Size: 880 427 916 2514 184 2007 Fragments arranged by size: 2514 2007 916 880 427 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5106 Fragments arranged by size: 5106 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 3170 4886 5245 5404 518 Size: 648 1311 693 1716 359 159 2042 Fragments arranged by size: 2042 1716 1311 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 3719 3779 4188 4344 5021 6169 6414 Size: 786 1327 60 409 156 677 1148 245 Cuts at: 6414 1606 Size: 2120 Fragments arranged by size: 2120 1327 1148 786 677 409 245 156 60 NcoI C'CATG_G Cuts at: 513 1065 3771 513 Size: 552 2706 3670 Fragments arranged by size: 3670 2706 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6928 NheI G'CTAG_C Cuts at: 1052 2308 1052 Size: 1256 5672 NotI GC'GGCC_GC Cuts at: 4375 4375 Size: 6928 NspI r_CATG'y Cuts at: 1132 1229 3016 4286 5831 1132 Size: 97 1787 1270 1545 2229 Fragments arranged by size: 2229 1787 1545 1270 97 PasI CC'CwG_GG Cuts at: 1348 2635 1348 Size: 1287 5641 PciI A'CATG_T Cuts at: 3012 5827 3012 Size: 2815 4113 PflMI CCAn_nnn'nTGG Cuts at: 1108 3323 3685 1108 Size: 2215 362 4351 Fragments arranged by size: 4351 2215 362 PfoI T'CCnGG_A Cuts at: 3109 3109 Size: 6928 PmeI GTTT'AAAC Cuts at: 4232 4232 Size: 6928 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4997 5029 6510 6542 4997 Size: 32 1481 32 5383 Fragments arranged by size: 5383 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3858 3858 Size: 6928 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6928 PsiI TTA'TAA Cuts at: 4498 4498 Size: 6928 PstI C_TGCA'G Cuts at: 830 2407 3787 4099 4280 830 Size: 1577 1380 312 181 3478 Fragments arranged by size: 3478 1577 1380 312 181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6928 PvuII CAG'CTG Cuts at: 2392 3719 3779 2392 Size: 1327 60 5541 Fragments arranged by size: 5541 1327 60 SalI G'TCGA_C Cuts at: 1936 4270 1936 Size: 2334 4594 SanDI GG'GwC_CC Cuts at: 3858 3858 Size: 6928 SbfI CC_TGCA'GG Cuts at: 4280 4280 Size: 6928 ScaI AGT'ACT Cuts at: 5176 6888 5176 Size: 1712 5216 SexAI A'CCwGG_T Cuts at: 3972 3972 Size: 6928 SfcI C'TryA_G Cuts at: 826 1045 2100 2403 2708 3783 4095 4113 4276 Size: 219 1055 303 305 1075 312 18 163 Cuts at: 4276 5411 6092 6283 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 1055 681 312 305 303 219 191 163 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6928 SmaI CCC'GGG Cuts at: 1872 4255 1872 Size: 2383 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2852 2861 3124 5032 5933 6195 Size: 197 926 909 9 263 1908 901 262 Cuts at: 6195 820 Size: 1553 Fragments arranged by size: 1908 1553 926 909 901 263 262 197 9 SnaBI TAC'GTA Cuts at: 493 4719 493 Size: 4226 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6928 SphI G_CATG'C Cuts at: 1132 4286 1132 Size: 3154 3774 SspI AAT'ATT Cuts at: 5 52 4852 5 Size: 47 4800 2081 Fragments arranged by size: 4800 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6928 StyI C'CwwG_G Cuts at: 513 1065 2140 3771 4218 4367 513 Size: 552 1075 1631 447 149 3074 Fragments arranged by size: 3074 1631 1075 552 447 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4955 4972 5125 5310 5755 325 Size: 1255 3375 17 153 185 445 1498 Fragments arranged by size: 3375 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2206 3899 5174 Size: 274 80 33 51 1443 229 1693 1275 Cuts at: 5174 6886 96 Size: 1712 138 Fragments arranged by size: 1712 1693 1443 1275 274 229 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3520 3552 3634 3666 4118 4150 6888 6920 3520 Size: 32 82 32 452 32 2738 32 3528 Fragments arranged by size: 3528 2738 452 82 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6928 XbaI T'CTAG_A Cuts at: 4264 4264 Size: 6928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3169 3682 3169 Size: 513 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6928 XmaI C'CCGG_G Cuts at: 1870 4253 1870 Size: 2383 4545 XmnI GAAnn'nnTTC Cuts at: 3188 5057 3188 Size: 1869 5059 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4736 276 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI