(Circular) (Six-base) MAPSORT of: pFN21AB9568.seq Check: 2532 from: 1 to: 9040 pFN21AB9568 9040 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3545 3545 Size: 9040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2772 4449 6850 278 Size: 53 83 186 936 1236 1677 2401 2468 Fragments arranged by size: 2468 2401 1677 1236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6383 1937 Size: 4446 4594 Acc65I G'GTAC_C Cuts at: 4934 6361 4934 Size: 1427 7613 AclI AA'CG_TT Cuts at: 1563 2655 3588 7167 7540 1563 Size: 1092 933 3579 373 3063 Fragments arranged by size: 3579 3063 1092 933 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2732 3434 5421 5640 7103 8487 8755 Size: 995 105 702 1987 219 1463 1384 268 Cuts at: 8755 1632 Size: 1917 Fragments arranged by size: 1987 1917 1463 1384 995 702 268 219 105 AfeI AGC'GCT Cuts at: 4581 4921 4581 Size: 340 8700 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8843 AflIII A'CryG_T Cuts at: 1203 7939 8916 1203 Size: 6736 977 1327 Fragments arranged by size: 6736 1327 977 AgeI A'CCGG_T Cuts at: 7871 7871 Size: 9040 AhdI GACnn_n'nnGTC Cuts at: 7769 7769 Size: 9040 AleI CACnn'nnGTG Cuts at: 1166 6047 1166 Size: 4881 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4256 4791 6246 1184 Size: 3072 535 1455 3978 Fragments arranged by size: 3978 3072 1455 535 AlwNI CAG_nnn'CTG Cuts at: 2682 2710 3200 3700 4306 5183 5909 6783 8355 Size: 28 490 500 606 877 726 874 1572 Cuts at: 8355 2682 Size: 3367 Fragments arranged by size: 3367 1572 877 874 726 606 500 490 28 ApaI G_GGCC'C Cuts at: 3047 4262 3047 Size: 1215 7825 ApaLI G'TGCA_C Cuts at: 6050 7097 8253 6050 Size: 1047 1156 6837 Fragments arranged by size: 6837 1156 1047 ApoI r'AATT_y Cuts at: 1493 4503 5492 6349 6575 6727 8836 1493 Size: 3010 989 857 226 152 2109 1697 Fragments arranged by size: 3010 2109 1697 989 857 226 152 AseI AT'TA_AT Cuts at: 160 1032 7594 160 Size: 872 6562 1606 Fragments arranged by size: 6562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9040 AvaI C'yCGr_G Cuts at: 1870 1943 3906 5102 5282 6365 1870 Size: 73 1963 1196 180 1083 4545 Fragments arranged by size: 4545 1963 1196 1083 180 73 BamHI G'GATC_C Cuts at: 3094 4132 6370 6737 3094 Size: 1038 2238 367 5397 Fragments arranged by size: 5397 2238 1038 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2564 3376 3608 4934 5038 Size: 325 217 627 777 812 232 1326 104 Cuts at: 5038 5072 5224 6361 7816 618 Size: 34 152 1137 1455 1842 Fragments arranged by size: 1842 1455 1326 1137 812 777 627 325 232 217 152 104 34 BanII G_rGCy'C Cuts at: 1391 2232 2502 3047 3684 4149 4224 4262 4614 Size: 841 270 545 637 465 75 38 352 Cuts at: 4614 4709 4829 4983 5342 5582 6359 1391 Size: 95 120 154 359 240 777 4072 Fragments arranged by size: 4072 841 777 637 545 465 359 352 270 240 154 120 95 75 38 BbeI G_GCGC'C Cuts at: 5042 5228 5042 Size: 186 8854 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2887 928 Size: 971 988 7081 Fragments arranged by size: 7081 988 971 BbvCI CC'TCA_GC Cuts at: 4916 4916 Size: 9040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2674 2708 4334 4368 5722 5756 7231 Size: 34 737 34 1626 34 1354 34 1475 Cuts at: 7231 7265 1903 Size: 34 3678 Fragments arranged by size: 3678 1626 1475 1354 737 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5408 6932 8142 5408 Size: 1524 1210 6306 Fragments arranged by size: 6306 1524 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9040 BfrBI ATG'CAT Cuts at: 2371 2371 Size: 9040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2402 5126 5262 Size: 107 122 71 631 605 730 2724 136 Cuts at: 5262 7651 136 Size: 2389 1525 Fragments arranged by size: 2724 2389 1525 730 631 605 136 122 107 71 BglII A'GATC_T Cuts at: 9035 9035 Size: 9040 BlpI GC'TnA_GC Cuts at: 4049 4328 4619 5910 6457 4049 Size: 279 291 1291 547 6632 Fragments arranged by size: 6632 1291 547 291 279 Bme1580I G_kGCm'C Cuts at: 1790 3047 3262 3611 4262 6054 7101 8257 1790 Size: 1257 215 349 651 1792 1047 1156 2573 Fragments arranged by size: 2573 1792 1257 1156 1047 651 349 215 BmgBI CAC'GTC Cuts at: 1318 4652 1318 Size: 3334 5706 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 4946 5075 Size: 416 53 83 399 24 1388 2141 129 Cuts at: 5075 6771 7729 442 Size: 1696 958 1753 Fragments arranged by size: 2141 1753 1696 1388 958 416 399 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 4238 4846 5596 3911 Size: 327 608 750 7355 Fragments arranged by size: 7355 750 608 327 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 4145 4185 4521 4539 4967 5341 5353 Size: 1046 1860 40 336 18 428 374 12 Cuts at: 5353 5703 7700 1239 Size: 350 1997 2579 Fragments arranged by size: 2579 1997 1860 1046 428 374 350 336 40 18 12 Bpu10I CC'TnA_GC Cuts at: 4608 4916 6005 6273 4608 Size: 308 1089 268 7375 Fragments arranged by size: 7375 1089 308 268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 3311 7165 8030 8328 2111 Size: 1200 3854 865 298 2823 Fragments arranged by size: 3854 2823 1200 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4011 7703 882 Size: 617 2512 3692 2219 Fragments arranged by size: 3692 2512 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3373 3394 6831 493 Size: 711 2169 21 3437 2702 Fragments arranged by size: 3437 2702 2169 711 21 BsaBI GATnn'nnATC Cuts at: 2684 9034 2684 Size: 6350 2690 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2769 4446 4754 5039 Size: 53 83 186 936 1236 1677 308 285 Cuts at: 5039 5225 6847 7229 7864 275 Size: 186 1622 382 635 1451 Fragments arranged by size: 1677 1622 1451 1236 936 635 382 308 285 186 186 83 53 BsaWI w'CCGG_w Cuts at: 2507 3619 4904 4931 5026 6111 7473 7871 8145 Size: 1112 1285 27 95 1085 1362 398 274 Cuts at: 8145 8292 8703 2507 Size: 147 411 2844 Fragments arranged by size: 2844 1362 1285 1112 1085 411 398 274 147 95 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4008 4038 4511 4541 5954 Size: 30 165 30 2605 30 473 30 1413 Cuts at: 5954 5984 1178 Size: 30 4234 Fragments arranged by size: 4234 2605 1413 473 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2099 3079 3487 4279 4819 4831 5120 Size: 838 75 980 408 792 540 12 289 Cuts at: 5120 5344 1186 Size: 224 4882 Fragments arranged by size: 4882 980 838 792 540 408 289 224 75 12 BseYI C'CCAG_C Cuts at: 2672 2703 2991 3693 3879 3986 4262 4355 4627 Size: 31 288 702 186 107 276 93 272 Cuts at: 4627 4885 5045 5156 8243 8739 2672 Size: 258 160 111 3087 496 2973 Fragments arranged by size: 3087 2973 702 496 288 276 272 258 186 160 111 107 93 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2825 3036 3449 4771 1163 Size: 573 345 744 211 413 1322 5432 Fragments arranged by size: 5432 1322 744 573 413 345 211 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4897 6490 7251 8279 1540 Size: 275 186 2896 1593 761 1028 2301 Fragments arranged by size: 2896 2301 1593 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 4055 4829 4983 5390 5759 6054 7101 7186 Size: 1823 774 154 407 369 295 1047 85 Cuts at: 7186 8257 2232 Size: 1071 3015 Fragments arranged by size: 3015 1823 1071 1047 774 407 369 295 154 85 BsiWI C'GTAC_G Cuts at: 3000 3000 Size: 9040 BsmI GAATG_Cn' Cuts at: 2530 5803 6551 6644 2530 Size: 3273 748 93 4926 Fragments arranged by size: 4926 3273 748 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2389 3396 4448 4667 2389 Size: 1007 1052 219 6762 Fragments arranged by size: 6762 1052 1007 219 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2502 3047 3262 3611 3684 4055 Size: 399 442 270 545 215 349 73 371 Cuts at: 4055 4149 4224 4262 4614 4709 4829 4983 5342 Size: 94 75 38 352 95 120 154 359 Cuts at: 5342 5390 5582 5759 6054 6359 7101 7186 8257 Size: 48 192 177 295 305 742 85 1071 Cuts at: 8257 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 545 442 399 371 359 352 349 305 295 270 215 192 177 154 120 95 94 85 75 73 48 38 BspEI T'CCGG_A Cuts at: 2507 3619 6111 8703 2507 Size: 1112 2492 2592 2844 Fragments arranged by size: 2844 2592 2492 1112 BspHI T'CATG_A Cuts at: 6315 6929 8659 6315 Size: 614 1730 6696 Fragments arranged by size: 6696 1730 614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 3471 3545 4428 6395 844 Size: 1259 1368 74 883 1967 3489 Fragments arranged by size: 3489 1967 1368 1259 883 74 BsrBI CCG'CTC Cuts at: 1394 1823 2754 3655 3661 4893 6927 1394 Size: 429 931 901 6 1232 2034 3507 Fragments arranged by size: 3507 2034 1232 931 901 429 6 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 5944 7535 7709 65 Size: 1183 1179 3517 1591 174 1396 Fragments arranged by size: 3517 1591 1396 1183 1179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 2595 2698 4037 4896 5978 7684 Size: 110 262 523 103 1339 859 1082 1706 Cuts at: 7684 7871 1700 Size: 187 2869 Fragments arranged by size: 2869 1706 1339 1082 859 523 262 187 110 103 BsrGI T'GTAC_A Cuts at: 96 2634 2937 96 Size: 2538 303 6199 Fragments arranged by size: 6199 2538 303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9040 BssSI C'ACGA_G Cuts at: 7100 8112 7100 Size: 1012 8028 BstAPI GCAn_nnn'nTGC Cuts at: 794 2682 794 Size: 1888 7152 BstBI TT'CG_AA Cuts at: 7844 7844 Size: 9040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9040 BstXI CCAn_nnnn'nTGG Cuts at: 4187 4873 5638 4187 Size: 686 765 7589 Fragments arranged by size: 7589 765 686 BstYI r'GATC_y Cuts at: 1972 2646 2679 2836 3094 3310 3598 4132 5854 Size: 674 33 157 258 216 288 534 1722 Cuts at: 5854 6370 6737 7122 7139 8591 8677 8689 9035 Size: 516 367 385 17 1452 86 12 346 Cuts at: 9035 1972 Size: 1977 Fragments arranged by size: 1977 1722 1452 674 534 516 385 367 346 288 258 216 157 86 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2017 2560 2947 4872 6234 513 Size: 552 939 13 543 387 1925 1362 3319 Fragments arranged by size: 3319 1925 1362 939 552 543 387 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 5536 487 Size: 683 56 812 3498 3991 Fragments arranged by size: 3991 3498 812 683 56 BtsI GCAGTG_nn' Cuts at: 2320 3181 3955 6235 6556 7350 7370 2320 Size: 861 774 2280 321 794 20 3990 Fragments arranged by size: 3990 2280 861 794 774 321 20 ClaI AT'CG_AT Cuts at: 1554 4494 1554 Size: 2940 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3340 3375 529 Size: 35 2776 35 6194 Fragments arranged by size: 6194 2776 35 35 DraI TTT'AAA Cuts at: 6344 6344 Size: 9040 DraIII CAC_nnn'GTG Cuts at: 2953 3376 3472 4690 2953 Size: 423 96 1218 7303 Fragments arranged by size: 7303 1218 423 96 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8047 809 Size: 507 6731 1802 Fragments arranged by size: 6731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2222 2757 3129 Size: 54 1425 50 275 18 392 535 372 Cuts at: 3129 3184 4894 5127 5628 5640 5691 5980 6487 Size: 55 1710 233 501 12 51 289 507 Cuts at: 6487 7376 9005 8 Size: 889 1629 43 Fragments arranged by size: 1710 1629 1425 889 535 507 501 392 372 289 275 233 55 54 51 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 4894 6487 1537 Size: 275 3082 1593 4090 Fragments arranged by size: 4090 3082 1593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3216 4837 6110 6970 1353 Size: 1863 1621 1273 860 3423 Fragments arranged by size: 3423 1863 1621 1273 860 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2768 3712 5088 5543 7627 8001 Size: 1064 138 1179 944 1376 455 2084 374 Cuts at: 8001 8147 387 Size: 146 1280 Fragments arranged by size: 2084 1376 1280 1179 1064 944 455 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 4827 4981 2230 Size: 2597 154 6289 Fragments arranged by size: 6289 2597 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2627 2732 3434 4145 4185 4521 Size: 393 653 342 105 702 711 40 336 Cuts at: 4521 4539 4967 5341 5353 5421 5640 5703 7103 Size: 18 428 374 12 68 219 63 1400 Cuts at: 7103 7700 8487 8755 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1400 787 711 702 653 597 428 393 374 342 336 268 219 105 68 63 40 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2108 3202 3872 4248 6020 2108 Size: 1094 670 376 1772 5128 Fragments arranged by size: 5128 1772 1094 670 376 EcoO109I rG'GnC_Cy Cuts at: 2404 3043 3044 3250 3363 3486 3506 3527 4058 Size: 639 1 206 113 123 20 21 531 Cuts at: 4058 4258 4259 4480 5110 5118 5616 6001 2404 Size: 200 1 221 630 8 498 385 5443 Fragments arranged by size: 5443 639 630 531 498 385 221 206 200 123 113 21 20 8 1 1 EcoRI G'AATT_C Cuts at: 6349 6727 6349 Size: 378 8662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6124 6124 Size: 9040 FseI GG_CCGG'CC Cuts at: 4900 4900 Size: 9040 FspI TGC'GCA Cuts at: 7546 7546 Size: 9040 HaeII r_GCGC'y Cuts at: 1678 2397 3229 4583 4923 5042 5228 8187 1678 Size: 719 832 1354 340 119 186 2959 2531 Fragments arranged by size: 2959 2531 1354 832 719 340 186 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3328 3360 4666 4698 4733 Size: 32 135 32 1756 32 1306 32 35 Cuts at: 4733 4765 5954 5986 6076 6108 7681 7713 7755 Size: 32 1189 32 90 32 1573 32 42 Cuts at: 7755 7787 1373 Size: 32 2626 Fragments arranged by size: 2626 1756 1573 1306 1189 135 90 42 35 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6298 6384 6630 669 Size: 988 281 4360 86 246 3079 Fragments arranged by size: 4360 3079 988 281 246 86 HindIII A'AGCT_T Cuts at: 748 2192 748 Size: 1444 7596 HpaI GTT'AAC Cuts at: 6630 6630 Size: 9040 KasI G'GCGC_C Cuts at: 5038 5224 5038 Size: 186 8854 KpnI G_GTAC'C Cuts at: 4938 6365 4938 Size: 1427 7613 MfeI C'AATT_G Cuts at: 6639 6639 Size: 9040 MluI A'CGCG_T Cuts at: 8916 8916 Size: 9040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3258 8154 8338 1305 Size: 1953 4896 184 2007 Fragments arranged by size: 4896 2007 1953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2224 5129 5630 5642 5693 Size: 54 1425 343 392 2905 501 12 51 Cuts at: 5693 10 Size: 3357 Fragments arranged by size: 3357 2905 1425 501 392 343 54 51 12 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2120 2213 2348 3396 5001 5555 Size: 648 891 63 93 135 1048 1605 554 Cuts at: 5555 5636 6047 6998 7357 7516 518 Size: 81 411 951 359 159 2042 Fragments arranged by size: 2042 1605 1048 951 891 648 554 411 359 159 135 93 81 63 MspA1I CmG'CkG Cuts at: 1606 3286 3846 4366 4442 4618 5167 5382 5668 Size: 1680 560 520 76 176 549 215 286 Cuts at: 5668 5909 6456 7133 8281 8526 1606 Size: 241 547 677 1148 245 2120 Fragments arranged by size: 2120 1680 1148 677 560 549 547 520 286 245 241 215 176 76 NaeI GCC'GGC Cuts at: 2074 2597 2700 4039 4898 2074 Size: 523 103 1339 859 6216 Fragments arranged by size: 6216 1339 859 523 103 NarI GG'CG_CC Cuts at: 5039 5225 5039 Size: 186 8854 NcoI C'CATG_G Cuts at: 513 1065 2004 2017 4872 513 Size: 552 939 13 2855 4681 Fragments arranged by size: 4681 2855 939 552 13 NdeI CA'TA_TG Cuts at: 387 2264 387 Size: 1877 7163 NgoMIV G'CCGG_C Cuts at: 2072 2595 2698 4037 4896 2072 Size: 523 103 1339 859 6216 Fragments arranged by size: 6216 1339 859 523 103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9040 NotI GC'GGCC_GC Cuts at: 6487 6487 Size: 9040 NsiI A_TGCA'T Cuts at: 2373 2373 Size: 9040 NspI r_CATG'y Cuts at: 1132 1229 2125 2309 2371 6398 7943 1132 Size: 97 896 184 62 4027 1545 2229 Fragments arranged by size: 4027 2229 1545 896 184 97 62 PasI CC'CwG_GG Cuts at: 1348 4306 4808 6070 1348 Size: 2958 502 1262 4318 Fragments arranged by size: 4318 2958 1262 502 PciI A'CATG_T Cuts at: 7939 7939 Size: 9040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 2308 4852 5517 1108 Size: 915 285 2544 665 4631 Fragments arranged by size: 4631 2544 915 665 285 PfoI T'CCnGG_A Cuts at: 2642 3313 4012 4122 6021 2642 Size: 671 699 110 1899 5661 Fragments arranged by size: 5661 1899 699 671 110 PmeI GTTT'AAAC Cuts at: 6344 6344 Size: 9040 PmlI CAC'GTG Cuts at: 3373 3394 3373 Size: 21 9019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7109 7141 8622 8654 7109 Size: 32 1481 32 7495 Fragments arranged by size: 7495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3363 3486 3527 4480 5110 3363 Size: 123 41 953 630 7293 Fragments arranged by size: 7293 953 630 123 41 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9040 PsiI TTA'TAA Cuts at: 6610 6610 Size: 9040 PspOMI G'GGCC_C Cuts at: 3043 4258 3043 Size: 1215 7825 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2471 2503 2471 Size: 32 9008 PstI C_TGCA'G Cuts at: 830 2327 2787 3856 4840 5262 5667 6392 830 Size: 1497 460 1069 984 422 405 725 3478 Fragments arranged by size: 3478 1497 1069 984 725 460 422 405 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9040 PvuII CAG'CTG Cuts at: 3286 4366 5668 5909 3286 Size: 1080 1302 241 6417 Fragments arranged by size: 6417 1302 1080 241 SacI G_AGCT'C Cuts at: 2232 4829 4983 2232 Size: 2597 154 6289 Fragments arranged by size: 6289 2597 154 SalI G'TCGA_C Cuts at: 1936 6382 1936 Size: 4446 4594 SanDI GG'GwC_CC Cuts at: 3363 3486 3527 3363 Size: 123 41 8876 Fragments arranged by size: 8876 123 41 SapI GCTCTTCn'nnn_ Cuts at: 3216 6110 3216 Size: 2894 6146 SbfI CC_TGCA'GG Cuts at: 5262 6392 5262 Size: 1130 7910 ScaI AGT'ACT Cuts at: 7288 9000 7288 Size: 1712 7328 SexAI A'CCwGG_T Cuts at: 2151 4020 2151 Size: 1869 7171 SfcI C'TryA_G Cuts at: 826 1045 2105 2323 2783 3192 3852 4836 5258 Size: 219 1060 218 460 409 660 984 422 Cuts at: 5258 5663 6388 7523 8204 8395 826 Size: 405 725 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 984 725 681 660 460 422 409 405 219 218 191 SfiI GGCCn_nnn'nGGCC Cuts at: 5126 5262 5126 Size: 136 8904 SfoI GGC'GCC Cuts at: 5040 5226 5040 Size: 186 8854 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9040 SmaI CCC'GGG Cuts at: 1872 5284 6367 1872 Size: 3412 1083 4545 Fragments arranged by size: 4545 3412 1083 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 3326 7144 8045 8307 820 Size: 197 926 183 1200 3818 901 262 1553 Fragments arranged by size: 3818 1553 1200 926 901 262 197 183 SnaBI TAC'GTA Cuts at: 493 6831 493 Size: 6338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9040 SphI G_CATG'C Cuts at: 1132 2125 6398 1132 Size: 993 4273 3774 Fragments arranged by size: 4273 3774 993 SrfI GCCC'GGGC Cuts at: 5284 5284 Size: 9040 SspI AAT'ATT Cuts at: 5 52 6964 5 Size: 47 6912 2081 Fragments arranged by size: 6912 2081 47 StuI AGG'CCT Cuts at: 1517 3737 5057 5256 1517 Size: 2220 1320 199 5301 Fragments arranged by size: 5301 2220 1320 199 StyI C'CwwG_G Cuts at: 513 1065 2004 2017 3481 3631 4872 5430 5790 Size: 552 939 13 1464 150 1241 558 360 Cuts at: 5790 5817 6150 6479 513 Size: 27 333 329 3074 Fragments arranged by size: 3074 1464 1241 939 558 552 360 333 329 150 27 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3760 4840 7067 7084 7237 7422 7867 Size: 1255 2180 1080 2227 17 153 185 445 Cuts at: 7867 325 Size: 1498 Fragments arranged by size: 2227 2180 1498 1255 1080 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2634 2937 3354 Size: 274 80 33 51 1443 657 303 417 Cuts at: 3354 7286 8998 96 Size: 3932 1712 138 Fragments arranged by size: 3932 1712 1443 657 417 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2240 2272 3601 3633 4511 4543 4894 4926 5643 Size: 32 1329 32 878 32 351 32 717 Cuts at: 5643 5675 6230 6262 9000 9032 2240 Size: 32 555 32 2738 32 2248 Fragments arranged by size: 2738 2248 1329 878 717 555 351 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3490 1316 Size: 2174 6866 XbaI T'CTAG_A Cuts at: 6376 6376 Size: 9040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2988 3290 3922 4028 5815 2988 Size: 302 632 106 1787 6213 Fragments arranged by size: 6213 1787 632 302 106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9040 XmaI C'CCGG_G Cuts at: 1870 5282 6365 1870 Size: 3412 1083 4545 Fragments arranged by size: 4545 3412 1083 XmnI GAAnn'nnTTC Cuts at: 6104 7169 6104 Size: 1065 7975 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2770 4447 6848 276 Size: 53 83 186 936 1236 1677 2401 2468 Fragments arranged by size: 2468 2401 1677 1236 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BstZ17I Bsu36I EcoRV FspAI NruI PacI PspXI RsrII SacII SwaI