Restriction map for
pFN21AB9566
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BanI BbeI
BbvCI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SmaI SnaBI SpeI SphI Sse8387I
SspI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 1564 AAATTC [GA]^AATT[CT]
AgeI 2327 ACCGGT A^CCGGT
AhdI 697 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1024 GTGCAC G^TGCAC
AlwNI 56 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1024 GTGCAC G^TGCAC
ApoI 1564 AAATTC [GA]^AATT[CT]
AspEI 697 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 594 GGACC G^G[AT]CC
BamHI 584 GGATCC G^GATCC
BanII 1684 GAGCTC G[GA]GC[CT]^C
BbrPI 2038 CACGTG CAC^GTG
BcgI 1344 CGAACACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1845 GGATAC GTATCC(6/5)
BsaAI 2038 CACGTG [CT]AC^GT[GA]
BseRI 988 GAGGAG GAGGAG(10/8)
BsgI 1858 GTGCAG GTGCAG(16/14)
BsmAI 1579 GAGAC GTCTC(1/5)
BsmFI 337 GTCCC GGGAC(10/14)
BsmI 1465 GAATGC GAATGC(1/-1)
Bsp1407I 758 TGTACA T^GTACA
BspHI 471 TCATGA T^CATGA
BsrFI 2327 ACCGGT [GA]^CCGG[CT]
BsrGI 758 TGTACA T^GTACA
BssSI 1027 CACGAG CACGAG(-5/-1)
BstBI 1926 TTCGAA TT^CGAA
Cfr10I 2327 ACCGGT [GA]^CCGG[CT]
DraIII 2219 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 692 CCATGG C^C[GA][CT]GG
Eam1105I 697 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1761 CTCTTC CTCTTC(1/4)
EcoRV 238 GATATC GAT^ATC
Ksp632I 1761 CTCTTC CTCTTC(1/4)
NcoI 692 CCATGG C^CATGG
NdeI 2209 CATATG CA^TATG
NspV 1926 TTCGAA TT^CGAA
PflMI 1608 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2327 ACCGGT A^CCGGT
PmaCI 2038 CACGTG CAC^GTG
PmlI 2038 CACGTG CAC^GTG
PshAI 1822 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1583 CAGCTG CAG^CTG
RcaI 471 TCATGA T^CATGA
SacI 1684 GAGCTC GAGCT^C
SfuI 1926 TTCGAA TT^CGAA
SgrAI 2326 CACCGGTG C[GA]^CCGG[CT]G
SspBI 758 TGTACA T^GTACA
StuI 2017 AGGCCT AGG^CCT
SwaI 935 ATTTAAAT ATTT^AAAT
Van91I 1608 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 881 CCAGGGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 805 GTAGAC GT^[AC][GT]AC
2075 GTAGAC
AocI 742 CCTGAGG CC^T[ACGT]AGG
2276 CCTCAGG
AspHI 1024 GTGCAC G[AT]GC[AT]^C
1684 GAGCTC
BpmI 779 CTGGAG CTGGAG(16/14)
1423 CTGGAG
BsaBI 79 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1252 GATGGACATC
BsaWI 1118 ACCGGA [AT]^CCGG[AT]
2327 ACCGGT
BsiHKAI 1024 GTGCAC G[AT]GC[AT]^C
1684 GAGCTC
BsiYI 1608 CCACCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1707 CCATACTGGGG
BslI 1608 CCACCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1707 CCATACTGGGG
BsrDI 328 CATTGC GCAATG(2/0)
1909 CATTGC
BstAPI 547 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2379 GCACATAATGC
Bsu36I 742 CCTGAGG CC^T[ACGT]AGG
2276 CCTCAGG
Cfr13I 594 GGACC G^G[ACGT]CC
1246 GGCCC
Eco81I 742 CCTGAGG CC^T[ACGT]AGG
2276 CCTCAGG
EcoT14I 644 CCTTGG C^C[AT][AT]GG
692 CCATGG
HapII 1119 CCGG C^CGG
2328 CCGG
HpaII 1119 CCGG C^CGG
2328 CCGG
MamI 79 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1252 GATGGACATC
MspI 1119 CCGG C^CGG
2328 CCGG
NlaIV 584 GGATCC GG[ACGT]^[ACGT]CC
1540 GGAGCC
PstI 998 CTGCAG CTGCA^G
1754 CTGCAG
Sau96I 594 GGACC G^G[ACGT]CC
1246 GGCCC
SmlI 1932 CTCAAG C^T[CT][GA]AG
2029 CTCAAG
StyI 644 CCTTGG C^C[AT][AT]GG
692 CCATGG
TaqI 1306 TCGA T^CGA
1927 TCGA
TthHB8I 1306 TCGA T^CGA
1927 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
Asp700I BbsI BbvI BclI BmyI
BpuAI BsaJI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII DraI EcoRII FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NspI
PleI RsaI Sau3AI ScaI ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI