(Circular) (Six-base) MAPSORT of: pFN21AB9566.seq Check: 2478 from: 1 to: 7120 pFN21AB9566 7120 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4930 278 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2811 4081 4463 1937 Size: 874 1270 382 4594 Fragments arranged by size: 4594 1270 874 382 Acc65I G'GTAC_C Cuts at: 4441 4441 Size: 7120 AclI AA'CG_TT Cuts at: 1563 5247 5620 1563 Size: 3684 373 3063 Fragments arranged by size: 3684 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 2358 2586 4011 4353 5183 6567 6835 Size: 702 24 228 1425 342 830 1384 268 Cuts at: 6835 1632 Size: 1917 Fragments arranged by size: 1917 1425 1384 830 702 342 268 228 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6923 AflIII A'CryG_T Cuts at: 1203 2398 2422 2440 2449 2458 2467 3999 4042 Size: 1195 24 18 9 9 9 1532 43 Cuts at: 4042 6019 6996 1203 Size: 1977 977 1327 Fragments arranged by size: 1977 1532 1327 1195 977 43 24 18 9 9 9 AgeI A'CCGG_T Cuts at: 4332 5951 4332 Size: 1619 5501 AhdI GACnn_n'nnGTC Cuts at: 2707 5849 2707 Size: 3142 3978 AleI CACnn'nnGTG Cuts at: 1166 3904 4335 1166 Size: 2738 431 3951 Fragments arranged by size: 3951 2738 431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3888 4085 1184 Size: 2704 197 4219 Fragments arranged by size: 4219 2704 197 AlwNI CAG_nnn'CTG Cuts at: 2066 4863 6435 2066 Size: 2797 1572 2751 Fragments arranged by size: 2797 2751 1572 ApaLI G'TGCA_C Cuts at: 3029 5177 6333 3029 Size: 2148 1156 3816 Fragments arranged by size: 3816 2148 1156 ApoI r'AATT_y Cuts at: 1493 3569 4429 4655 4807 6916 1493 Size: 2076 860 226 152 2109 1697 Fragments arranged by size: 2109 2076 1697 860 226 152 AseI AT'TA_AT Cuts at: 160 1032 5674 160 Size: 872 4642 1606 Fragments arranged by size: 4642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 AvaI C'yCGr_G Cuts at: 1870 1943 4445 1870 Size: 73 2502 4545 Fragments arranged by size: 4545 2502 73 BamHI G'GATC_C Cuts at: 2589 4450 4817 2589 Size: 1861 367 4892 Fragments arranged by size: 4892 1861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4441 5896 618 Size: 325 217 627 2654 1455 1842 Fragments arranged by size: 2654 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3693 4439 1391 Size: 2302 746 4072 Fragments arranged by size: 4072 2302 746 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3217 3827 4226 4369 928 Size: 971 1318 610 399 143 3679 Fragments arranged by size: 3679 1318 971 610 399 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3338 3372 5311 5345 1903 Size: 34 1401 34 1939 34 3678 Fragments arranged by size: 3678 1939 1401 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3844 5012 6222 3844 Size: 1168 1210 4742 Fragments arranged by size: 4742 1210 1168 BclI T'GATC_A Cuts at: 1548 2089 2839 2860 1548 Size: 541 750 21 5808 Fragments arranged by size: 5808 750 541 21 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5731 136 Size: 107 122 71 631 605 4059 1525 Fragments arranged by size: 4059 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7115 7115 Size: 7120 BlpI GC'TnA_GC Cuts at: 4537 4537 Size: 7120 Bme1580I G_kGCm'C Cuts at: 1790 2240 3033 3145 5181 6337 1790 Size: 450 793 112 2036 1156 2573 Fragments arranged by size: 2573 2036 1156 793 450 112 BmgBI CAC'GTC Cuts at: 1318 2401 1318 Size: 1083 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4851 5809 Size: 416 53 83 399 24 2309 1125 958 Cuts at: 5809 442 Size: 1753 Fragments arranged by size: 2309 1753 1125 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7120 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2059 2059 Size: 7120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2805 3449 5780 1239 Size: 1566 644 2331 2579 Fragments arranged by size: 2579 2331 1566 644 Bpu10I CC'TnA_GC Cuts at: 2286 2286 Size: 7120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4019 5245 6110 6408 3922 Size: 97 1226 865 298 4634 Fragments arranged by size: 4634 1226 865 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5783 882 Size: 617 4284 2219 Fragments arranged by size: 4284 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4045 4911 493 Size: 711 2841 866 2702 Fragments arranged by size: 2841 2702 866 711 BsaBI GATnn'nnATC Cuts at: 2088 3261 7114 2088 Size: 1173 3853 2094 Fragments arranged by size: 3853 2094 1173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4927 5309 5944 275 Size: 53 83 186 936 3394 382 635 1451 Fragments arranged by size: 3394 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3123 4332 5553 5951 6225 6372 6783 3123 Size: 1209 1221 398 274 147 411 3460 Fragments arranged by size: 3460 1221 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3713 3743 1178 Size: 30 165 30 2310 30 4555 Fragments arranged by size: 4555 2310 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3008 1186 Size: 1822 5298 BseYI C'CCAG_C Cuts at: 6323 6819 6323 Size: 496 6624 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3884 1163 Size: 573 2148 4399 Fragments arranged by size: 4399 2148 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4570 5331 6359 1540 Size: 275 186 2569 761 1028 2301 Fragments arranged by size: 2569 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3033 3693 5181 5266 6337 3033 Size: 660 1488 85 1071 3816 Fragments arranged by size: 3816 1488 1071 660 85 BsmI GAATG_Cn' Cuts at: 3476 4631 4724 3476 Size: 1155 93 5872 Fragments arranged by size: 5872 1155 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 3033 3145 3693 4439 5181 5266 Size: 399 450 793 112 548 746 742 85 Cuts at: 5266 6337 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 793 746 742 548 450 399 112 85 BspEI T'CCGG_A Cuts at: 6783 6783 Size: 7120 BspHI T'CATG_A Cuts at: 2476 5009 6739 2476 Size: 2533 1730 2857 Fragments arranged by size: 2857 2533 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4475 844 Size: 3631 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5007 1394 Size: 429 3184 3507 Fragments arranged by size: 3507 3184 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2332 3913 5615 5789 65 Size: 1183 1084 1581 1702 174 1396 Fragments arranged by size: 1702 1581 1396 1183 1084 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4332 5764 5951 1700 Size: 110 2522 1432 187 2869 Fragments arranged by size: 2869 2522 1432 187 110 BsrGI T'GTAC_A Cuts at: 96 2763 96 Size: 2667 4453 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7120 BssSI C'ACGA_G Cuts at: 3032 5180 6192 3032 Size: 2148 1012 3960 Fragments arranged by size: 3960 2148 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2558 4390 794 Size: 1764 1832 3524 Fragments arranged by size: 3524 1832 1764 BstBI TT'CG_AA Cuts at: 3932 5924 3932 Size: 1992 5128 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7120 BstYI r'GATC_y Cuts at: 1972 2589 4105 4150 4450 4817 5202 5219 6671 Size: 617 1516 45 300 367 385 17 1452 Cuts at: 6671 6757 6769 7115 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1516 1452 617 385 367 346 300 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2748 4282 2748 Size: 1534 5586 BtgI C'CryG_G Cuts at: 513 1065 2004 2697 513 Size: 552 939 693 4936 Fragments arranged by size: 4936 939 693 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3489 487 Size: 683 56 2263 4118 Fragments arranged by size: 4118 2263 683 56 BtsI GCAGTG_nn' Cuts at: 3132 4636 5430 5450 3132 Size: 1504 794 20 4802 Fragments arranged by size: 4802 1504 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7085 DraI TTT'AAA Cuts at: 2773 2943 4291 4424 2773 Size: 170 1348 133 5469 Fragments arranged by size: 5469 1348 170 133 DraIII CAC_nnn'GTG Cuts at: 4229 4229 Size: 7120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6127 809 Size: 507 4811 1802 Fragments arranged by size: 4811 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4567 5456 7085 Size: 54 1425 50 275 18 2737 889 1629 Cuts at: 7085 8 Size: 43 Fragments arranged by size: 2737 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4567 1537 Size: 275 2755 4090 Fragments arranged by size: 4090 2755 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3772 5050 1353 Size: 2419 1278 3423 Fragments arranged by size: 3423 2419 1278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5707 6081 6227 387 Size: 1064 138 4118 374 146 1280 Fragments arranged by size: 4118 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3691 3691 Size: 7120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2358 2586 2805 3449 4011 4353 Size: 393 702 24 228 219 644 562 342 Cuts at: 4353 5183 5780 6567 6835 1239 Size: 830 597 787 268 1524 Fragments arranged by size: 1524 830 787 702 644 597 562 393 342 268 228 219 24 EcoRI G'AATT_C Cuts at: 4429 4807 4429 Size: 378 6742 EcoRV GAT'ATC Cuts at: 2245 2245 Size: 7120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2036 4372 2036 Size: 2336 4784 FspI TGC'GCA Cuts at: 5626 5626 Size: 7120 HaeII r_GCGC'y Cuts at: 1678 6267 1678 Size: 4589 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 2693 2725 3262 Size: 32 135 32 974 32 115 32 537 Cuts at: 3262 3294 4090 4122 5761 5793 5835 5867 1373 Size: 32 796 32 1639 32 42 32 2626 Fragments arranged by size: 2626 1639 974 796 537 135 115 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4464 4710 669 Size: 988 281 2526 246 3079 Fragments arranged by size: 3079 2526 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7120 HpaI GTT'AAC Cuts at: 4710 4710 Size: 7120 KpnI G_GTAC'C Cuts at: 4445 4445 Size: 7120 MfeI C'AATT_G Cuts at: 4719 4719 Size: 7120 MluI A'CGCG_T Cuts at: 6996 6996 Size: 7120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2416 2425 2434 2691 6234 6418 1305 Size: 1111 9 9 257 3543 184 2007 Fragments arranged by size: 3543 2007 1111 257 184 9 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5298 Fragments arranged by size: 5298 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2082 2153 2481 2759 3156 3651 3820 Size: 648 916 71 328 278 397 495 169 Cuts at: 3820 3904 3993 4162 4335 5078 5437 5596 518 Size: 84 89 169 173 743 359 159 2042 Fragments arranged by size: 2042 916 743 648 495 397 359 328 278 173 169 169 159 89 84 71 MspA1I CmG'CkG Cuts at: 1606 3014 3375 3590 4536 5213 6361 6606 1606 Size: 1408 361 215 946 677 1148 245 2120 Fragments arranged by size: 2120 1408 1148 946 677 361 245 215 NcoI C'CATG_G Cuts at: 513 1065 2004 2697 513 Size: 552 939 693 4936 Fragments arranged by size: 4936 939 693 552 NdeI CA'TA_TG Cuts at: 387 4215 387 Size: 3828 3292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7120 NotI GC'GGCC_GC Cuts at: 4567 4567 Size: 7120 NspI r_CATG'y Cuts at: 1132 1229 2426 2444 2453 2462 2471 3788 3875 Size: 97 1197 18 9 9 9 1317 87 Cuts at: 3875 4003 4478 6023 1132 Size: 128 475 1545 2229 Fragments arranged by size: 2229 1545 1317 1197 475 128 97 87 18 9 9 9 PasI CC'CwG_GG Cuts at: 1348 2886 1348 Size: 1538 5582 PciI A'CATG_T Cuts at: 2422 2440 2449 2458 2467 3999 6019 2422 Size: 18 9 9 9 1532 2020 3523 Fragments arranged by size: 3523 2020 1532 18 9 9 9 PflMI CCAn_nnn'nTGG Cuts at: 1108 3619 1108 Size: 2511 4609 PmeI GTTT'AAAC Cuts at: 4424 4424 Size: 7120 PmlI CAC'GTG Cuts at: 4045 4045 Size: 7120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5189 5221 6702 6734 5189 Size: 32 1481 32 5575 Fragments arranged by size: 5575 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3831 1613 Size: 2218 4902 PsiI TTA'TAA Cuts at: 4690 4690 Size: 7120 PstI C_TGCA'G Cuts at: 830 3007 3763 4472 830 Size: 2177 756 709 3478 Fragments arranged by size: 3478 2177 756 709 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7120 PvuII CAG'CTG Cuts at: 3590 3590 Size: 7120 SacI G_AGCT'C Cuts at: 3693 3693 Size: 7120 SalI G'TCGA_C Cuts at: 1936 4462 1936 Size: 2526 4594 SbfI CC_TGCA'GG Cuts at: 4472 4472 Size: 7120 ScaI AGT'ACT Cuts at: 3743 4256 4343 5368 7080 3743 Size: 513 87 1025 1712 3783 Fragments arranged by size: 3783 1712 1025 513 87 SfcI C'TryA_G Cuts at: 826 1045 2808 3003 3759 4272 4468 5603 6284 Size: 219 1763 195 756 513 196 1135 681 Cuts at: 6284 6475 826 Size: 191 1471 Fragments arranged by size: 1763 1471 1135 756 681 513 219 196 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 SgrAI Cr'CCGG_yG Cuts at: 1700 4332 1700 Size: 2632 4488 SmaI CCC'GGG Cuts at: 1872 4447 1872 Size: 2575 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4034 5224 6125 6387 820 Size: 197 926 1994 97 1190 901 262 1553 Fragments arranged by size: 1994 1553 1190 926 901 262 197 97 SnaBI TAC'GTA Cuts at: 493 4911 493 Size: 4418 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7120 SphI G_CATG'C Cuts at: 1132 4478 1132 Size: 3346 3774 SspI AAT'ATT Cuts at: 5 52 5044 5 Size: 47 4992 2081 Fragments arranged by size: 4992 2081 47 StuI AGG'CCT Cuts at: 1517 4024 1517 Size: 2507 4613 StyI C'CwwG_G Cuts at: 513 1065 2004 2649 2697 4559 513 Size: 552 939 645 48 1862 3074 Fragments arranged by size: 3074 1862 939 645 552 48 SwaI ATTT'AAAT Cuts at: 2943 2943 Size: 7120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5147 5164 5317 5502 5947 325 Size: 1255 3567 17 153 185 445 1498 Fragments arranged by size: 3567 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2107 2763 3377 Size: 274 80 33 51 1443 130 656 614 Cuts at: 3377 3741 4254 4341 5366 7078 96 Size: 364 513 87 1025 1712 138 Fragments arranged by size: 1712 1443 1025 656 614 513 364 274 138 130 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4289 4321 7080 7112 4289 Size: 32 2759 32 4297 Fragments arranged by size: 4297 2759 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7120 XbaI T'CTAG_A Cuts at: 4456 4456 Size: 7120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2893 2893 Size: 7120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7120 XmaI C'CCGG_G Cuts at: 1870 4445 1870 Size: 2575 4545 XmnI GAAnn'nnTTC Cuts at: 2229 3491 4355 4366 5249 2229 Size: 1262 864 11 883 4100 Fragments arranged by size: 4100 1262 883 864 11 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4928 276 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI