Restriction map for
pFN21AB9566
(Linear) (Six-base) MAPSORT of: KIBB9566.seq Check: 2136 from: 1 to: 2415
KIBB9566.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 806 2076 2415
Size: 806 1270 339
Fragments arranged by size:
1270 806 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 353 581 2006 2348 2415
Size: 329 24 228 1425 342 67
Fragments arranged by size:
1425 342 329 228 67 24
AflIII A'CryG_T
Cuts at: 0 393 417 435 444 453 462 1994 2037
Size: 393 24 18 9 9 9 1532 43
Cuts at: 2037 2415
Size: 378
Fragments arranged by size:
1532 393 378 43 24 18 9 9
9
AgeI A'CCGG_T
Cuts at: 0 2327 2415
Size: 2327 88
AhdI GACnn_n'nnGTC
Cuts at: 0 702 2415
Size: 702 1713
AleI CACnn'nnGTG
Cuts at: 0 1899 2330 2415
Size: 1899 431 85
Fragments arranged by size:
1899 431 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1883 2080 2415
Size: 1883 197 335
Fragments arranged by size:
1883 335 197
AlwNI CAG_nnn'CTG
Cuts at: 0 61 2415
Size: 61 2354
ApaLI G'TGCA_C
Cuts at: 0 1024 2415
Size: 1024 1391
ApoI r'AATT_y
Cuts at: 0 1564 2415
Size: 1564 851
BamHI G'GATC_C
Cuts at: 0 584 2415
Size: 584 1831
BanII G_rGCy'C
Cuts at: 0 1688 2415
Size: 1688 727
BbsI GAAGACnn'nnnn_
Cuts at: 0 1212 1822 2221 2364 2415
Size: 1212 610 399 143 51
Fragments arranged by size:
1212 610 399 143 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1333 1367 2415
Size: 1333 34 1048
Fragments arranged by size:
1333 1048 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1839 2415
Size: 1839 576
BclI T'GATC_A
Cuts at: 0 84 834 855 2415
Size: 84 750 21 1560
Fragments arranged by size:
1560 750 84 21
Bme1580I G_kGCm'C
Cuts at: 0 235 1028 1140 2415
Size: 235 793 112 1275
Fragments arranged by size:
1275 793 235 112
BmgBI CAC'GTC
Cuts at: 0 396 2415
Size: 396 2019
BmrI ACTGGGnnnn_n'
Cuts at: 0 1721 2415
Size: 1721 694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 54 2415
Size: 54 2361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1444 2415
Size: 800 644 971
Fragments arranged by size:
971 800 644
Bpu10I CC'TnA_GC
Cuts at: 0 281 2415
Size: 281 2134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1917 2014 2415
Size: 1917 97 401
Fragments arranged by size:
1917 401 97
BsaAI yAC'GTr
Cuts at: 0 2040 2415
Size: 2040 375
BsaBI GATnn'nnATC
Cuts at: 0 83 1256 2415
Size: 83 1173 1159
Fragments arranged by size:
1173 1159 83
BsaWI w'CCGG_w
Cuts at: 0 1118 2327 2415
Size: 1118 1209 88
Fragments arranged by size:
1209 1118 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1708 1738 2415
Size: 1708 30 677
Fragments arranged by size:
1708 677 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 2415
Size: 1003 1412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 2415
Size: 1879 536
BsiHKAI G_wGCw'C
Cuts at: 0 1028 1688 2415
Size: 1028 660 727
Fragments arranged by size:
1028 727 660
BsmI GAATG_Cn'
Cuts at: 0 1471 2415
Size: 1471 944
Bsp1286I G_dGCh'C
Cuts at: 0 235 1028 1140 1688 2415
Size: 235 793 112 548 727
Fragments arranged by size:
793 727 548 235 112
BspHI T'CATG_A
Cuts at: 0 471 2415
Size: 471 1944
BsrDI GCAATG_nn'
Cuts at: 0 327 1908 2415
Size: 327 1581 507
Fragments arranged by size:
1581 507 327
BsrFI r'CCGG_y
Cuts at: 0 2327 2415
Size: 2327 88
BsrGI T'GTAC_A
Cuts at: 0 758 2415
Size: 758 1657
BssSI C'ACGA_G
Cuts at: 0 1027 2415
Size: 1027 1388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 553 2385 2415
Size: 553 1832 30
Fragments arranged by size:
1832 553 30
BstBI TT'CG_AA
Cuts at: 0 1927 2415
Size: 1927 488
BstYI r'GATC_y
Cuts at: 0 584 2100 2145 2415
Size: 584 1516 45 270
Fragments arranged by size:
1516 584 270 45
Bsu36I CC'TnA_GG
Cuts at: 0 743 2277 2415
Size: 743 1534 138
Fragments arranged by size:
1534 743 138
BtgI C'CryG_G
Cuts at: 0 692 2415
Size: 692 1723
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1484 2415
Size: 1484 931
BtsI GCAGTG_nn'
Cuts at: 0 1127 2415
Size: 1127 1288
DraI TTT'AAA
Cuts at: 0 768 938 2286 2415
Size: 768 170 1348 129
Fragments arranged by size:
1348 768 170 129
DraIII CAC_nnn'GTG
Cuts at: 0 2224 2415
Size: 2224 191
EarI CTCTTCn'nnn_
Cuts at: 0 1767 2415
Size: 1767 648
EcoICRI GAG'CTC
Cuts at: 0 1686 2415
Size: 1686 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 353 581 800 1444 2006 2348 2415
Size: 329 24 228 219 644 562 342 67
Fragments arranged by size:
644 562 342 329 228 219 67 24
EcoRV GAT'ATC
Cuts at: 0 240 2415
Size: 240 2175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 31 2367 2415
Size: 31 2336 48
Fragments arranged by size:
2336 48 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 688 720 1257 1289 2085 2117
Size: 541 32 115 32 537 32 796 32
Cuts at: 2117 2415
Size: 298
Fragments arranged by size:
796 541 537 298 115 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 420 429 686 2415
Size: 411 9 9 257 1729
Fragments arranged by size:
1729 411 257 9 9
MslI CAynn'nnrTG
Cuts at: 0 77 148 476 754 1151 1646 1815 1899
Size: 77 71 328 278 397 495 169 84
Cuts at: 1899 1988 2157 2330 2415
Size: 89 169 173 85
Fragments arranged by size:
495 397 328 278 173 169 169 89
85 84 77 71
MspA1I CmG'CkG
Cuts at: 0 1009 1370 1585 2415
Size: 1009 361 215 830
Fragments arranged by size:
1009 830 361 215
NcoI C'CATG_G
Cuts at: 0 692 2415
Size: 692 1723
NdeI CA'TA_TG
Cuts at: 0 2210 2415
Size: 2210 205
NspI r_CATG'y
Cuts at: 0 421 439 448 457 466 1783 1870 1998
Size: 421 18 9 9 9 1317 87 128
Cuts at: 1998 2415
Size: 417
Fragments arranged by size:
1317 421 417 128 87 18 9 9
9
PasI CC'CwG_GG
Cuts at: 0 881 2415
Size: 881 1534
PciI A'CATG_T
Cuts at: 0 417 435 444 453 462 1994 2415
Size: 417 18 9 9 9 1532 421
Fragments arranged by size:
1532 421 417 18 9 9 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 1614 2415
Size: 1614 801
PmlI CAC'GTG
Cuts at: 0 2040 2415
Size: 2040 375
PshAI GACnn'nnGTC
Cuts at: 0 1826 2415
Size: 1826 589
PstI C_TGCA'G
Cuts at: 0 1002 1758 2415
Size: 1002 756 657
Fragments arranged by size:
1002 756 657
PvuII CAG'CTG
Cuts at: 0 1585 2415
Size: 1585 830
SacI G_AGCT'C
Cuts at: 0 1688 2415
Size: 1688 727
ScaI AGT'ACT
Cuts at: 0 1738 2251 2338 2415
Size: 1738 513 87 77
Fragments arranged by size:
1738 513 87 77
SfcI C'TryA_G
Cuts at: 0 803 998 1754 2267 2415
Size: 803 195 756 513 148
Fragments arranged by size:
803 756 513 195 148
SgrAI Cr'CCGG_yG
Cuts at: 0 2327 2415
Size: 2327 88
SmlI C'TyrA_G
Cuts at: 0 1932 2029 2415
Size: 1932 97 386
Fragments arranged by size:
1932 386 97
StuI AGG'CCT
Cuts at: 0 2019 2415
Size: 2019 396
StyI C'CwwG_G
Cuts at: 0 644 692 2415
Size: 644 48 1723
Fragments arranged by size:
1723 644 48
SwaI ATTT'AAAT
Cuts at: 0 938 2415
Size: 938 1477
TatI w'GTAC_w
Cuts at: 0 102 758 1372 1736 2249 2336 2415
Size: 102 656 614 364 513 87 79
Fragments arranged by size:
656 614 513 364 102 87 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2284 2316 2415
Size: 2284 32 99
Fragments arranged by size:
2284 99 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 2415
Size: 888 1527
XmnI GAAnn'nnTTC
Cuts at: 0 224 1486 2350 2361 2415
Size: 224 1262 864 11 54
Fragments arranged by size:
1262 864 224 54 11
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AleI AlfI AlwNI
ApaLI ApoI BamHI BanII BbsI BcgI BciVI BclI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII EarI EcoICRI
Eco57MI EcoRV FalI Hin4I MmeI MslI MspA1I NcoI
NdeI NspI PasI PciI PflMI PmlI PshAI PstI
PvuII SacI ScaI SfcI SgrAI SmlI StuI StyI
SwaI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbvCI BfrBI BglI BglII BlpI BmtI BsaI BsaHI
BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII
BstEII BstXI BstZ17I ClaI CspCI DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SspI TaqII Tth111I XbaI XhoI XmaI ZraI