(Circular) (Six-base) MAPSORT of: pFN21AB9560.seq Check: 2853 from: 1 to: 8113 pFN21AB9560 8113 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5923 278 Size: 53 83 186 936 4387 2468 Fragments arranged by size: 4387 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5456 1937 Size: 3519 4594 Acc65I G'GTAC_C Cuts at: 3855 4242 5434 3855 Size: 387 1192 6534 Fragments arranged by size: 6534 1192 387 AclI AA'CG_TT Cuts at: 1563 6240 6613 1563 Size: 4677 373 3063 Fragments arranged by size: 4677 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 3517 3889 4343 4430 4570 4577 4610 Size: 1041 844 372 454 87 140 7 33 Cuts at: 4610 4995 5182 6176 7560 7828 1632 Size: 385 187 994 1384 268 1917 Fragments arranged by size: 1917 1384 1041 994 844 454 385 372 268 187 140 87 33 7 AfeI AGC'GCT Cuts at: 2117 4337 2117 Size: 2220 5893 AflII C'TTAA_G Cuts at: 820 1017 2672 820 Size: 197 1655 6261 Fragments arranged by size: 6261 1655 197 AflIII A'CryG_T Cuts at: 1203 3510 5369 7012 7989 1203 Size: 2307 1859 1643 977 1327 Fragments arranged by size: 2307 1859 1643 1327 977 AgeI A'CCGG_T Cuts at: 2178 6944 2178 Size: 4766 3347 AhdI GACnn_n'nnGTC Cuts at: 5065 6842 5065 Size: 1777 6336 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4542 1184 Size: 3358 4755 AlwNI CAG_nnn'CTG Cuts at: 3416 4529 5856 7428 3416 Size: 1113 1327 1572 4101 Fragments arranged by size: 4101 1572 1327 1113 ApaLI G'TGCA_C Cuts at: 6170 7326 6170 Size: 1156 6957 ApoI r'AATT_y Cuts at: 1493 2230 2522 2752 2928 4020 4308 4580 5422 Size: 737 292 230 176 1092 288 272 842 Cuts at: 5422 5648 5800 7909 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 842 737 292 288 272 230 226 176 152 AseI AT'TA_AT Cuts at: 160 1032 3216 6667 160 Size: 872 2184 3451 1606 Fragments arranged by size: 3451 2184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8113 AvaI C'yCGr_G Cuts at: 1870 1943 2583 2874 5438 1870 Size: 73 640 291 2564 4545 Fragments arranged by size: 4545 2564 640 291 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5097 5130 5097 Size: 33 8080 BamHI G'GATC_C Cuts at: 3537 5443 5810 3537 Size: 1906 367 5840 Fragments arranged by size: 5840 1906 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2645 3855 4242 5434 6889 Size: 325 217 627 858 1210 387 1192 1455 Cuts at: 6889 618 Size: 1842 Fragments arranged by size: 1842 1455 1210 1192 858 627 387 325 217 BanII G_rGCy'C Cuts at: 1391 2249 2661 5126 5168 5432 1391 Size: 858 412 2465 42 264 4072 Fragments arranged by size: 4072 2465 858 412 264 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4101 928 Size: 971 2202 4940 Fragments arranged by size: 4940 2202 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6304 6338 1903 Size: 34 4367 34 3678 Fragments arranged by size: 4367 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6005 7215 6005 Size: 1210 6903 BclI T'GATC_A Cuts at: 1548 2950 5206 1548 Size: 1402 2256 4455 Fragments arranged by size: 4455 2256 1402 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6724 136 Size: 107 122 71 631 605 5052 1525 Fragments arranged by size: 5052 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8108 8108 Size: 8113 BlpI GC'TnA_GC Cuts at: 5530 5530 Size: 8113 Bme1580I G_kGCm'C Cuts at: 1790 6174 7330 1790 Size: 4384 1156 2573 Fragments arranged by size: 4384 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3513 1318 Size: 2195 5918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 2855 5844 Size: 416 53 83 399 24 917 521 2989 Cuts at: 5844 6802 442 Size: 958 1753 Fragments arranged by size: 2989 1753 958 917 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5034 5210 5034 Size: 176 7937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4136 6773 1239 Size: 2897 2637 2579 Fragments arranged by size: 2897 2637 2579 Bpu10I CC'TnA_GC Cuts at: 3033 3033 Size: 8113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 2699 4690 6238 7103 7401 2093 Size: 606 1991 1548 865 298 2805 Fragments arranged by size: 2805 1991 1548 865 606 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 3416 3606 6776 882 Size: 617 585 1332 190 3170 2219 Fragments arranged by size: 3170 2219 1332 617 585 190 BsaAI yAC'GTr Cuts at: 493 1204 4565 5904 493 Size: 711 3361 1339 2702 Fragments arranged by size: 3361 2702 1339 711 BsaBI GATnn'nnATC Cuts at: 4061 8107 4061 Size: 4046 4067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5920 6302 6937 275 Size: 53 83 186 936 4387 382 635 1451 Fragments arranged by size: 4387 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2178 6546 6944 7218 7365 7776 2178 Size: 4368 398 274 147 411 2515 Fragments arranged by size: 4368 2515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2391 2421 1178 Size: 30 165 30 988 30 6870 Fragments arranged by size: 6870 988 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2482 2565 2651 3293 4430 4517 1186 Size: 916 380 83 86 642 1137 87 4782 Fragments arranged by size: 4782 1137 916 642 380 87 86 83 BseYI C'CCAG_C Cuts at: 7316 7812 7316 Size: 496 7617 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5563 6324 7352 1540 Size: 275 186 3562 761 1028 2301 Fragments arranged by size: 3562 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2661 3494 5126 6174 6259 7330 2661 Size: 833 1632 1048 85 1071 3444 Fragments arranged by size: 3444 1632 1071 1048 833 85 BsmI GAATG_Cn' Cuts at: 2776 2972 5624 5717 2776 Size: 196 2652 93 5172 Fragments arranged by size: 5172 2652 196 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3519 4396 5396 3519 Size: 877 1000 6236 Fragments arranged by size: 6236 1000 877 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2249 2661 3494 5126 5168 5432 6174 Size: 399 459 412 833 1632 42 264 742 Cuts at: 6174 6259 7330 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1632 1071 833 742 459 412 399 264 85 42 BspEI T'CCGG_A Cuts at: 7776 7776 Size: 8113 BspHI T'CATG_A Cuts at: 2800 6002 7732 2800 Size: 3202 1730 3181 Fragments arranged by size: 3202 3181 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 3069 5468 844 Size: 1841 384 2399 3489 Fragments arranged by size: 3489 2399 1841 384 BsrBI CCG'CTC Cuts at: 1394 1823 6000 1394 Size: 429 4177 3507 Fragments arranged by size: 4177 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4359 4968 6608 6782 65 Size: 1183 3111 609 1640 174 1396 Fragments arranged by size: 3111 1640 1396 1183 609 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 2942 6757 6944 1700 Size: 110 368 764 3815 187 2869 Fragments arranged by size: 3815 2869 764 368 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8113 BssSI C'ACGA_G Cuts at: 6173 7185 6173 Size: 1012 7101 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8113 BstBI TT'CG_AA Cuts at: 6917 6917 Size: 8113 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8113 BstXI CCAn_nnnn'nTGG Cuts at: 5069 5354 5069 Size: 285 7828 BstYI r'GATC_y Cuts at: 1972 2282 3537 4466 5443 5810 6195 6212 7664 Size: 310 1255 929 977 367 385 17 1452 Cuts at: 7664 7750 7762 8108 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1255 977 929 385 367 346 310 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4100 4100 Size: 8113 BtgI C'CryG_G Cuts at: 513 1065 2004 3735 4026 513 Size: 552 939 1731 291 4600 Fragments arranged by size: 4600 1731 939 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7374 Fragments arranged by size: 7374 683 56 BtsI GCAGTG_nn' Cuts at: 2841 5209 5629 6423 6443 2841 Size: 2368 420 794 20 4511 Fragments arranged by size: 4511 2368 794 420 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3611 3646 529 Size: 35 3047 35 4996 Fragments arranged by size: 4996 3047 35 35 DraI TTT'AAA Cuts at: 2533 5417 2533 Size: 2884 5229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7120 809 Size: 507 5804 1802 Fragments arranged by size: 5804 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2721 3375 Size: 54 1425 50 275 18 177 714 654 Cuts at: 3375 3738 4125 5223 5560 6449 8078 8 Size: 363 387 1098 337 889 1629 43 Fragments arranged by size: 1629 1425 1098 889 714 654 387 363 337 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5560 1537 Size: 275 3748 4090 Fragments arranged by size: 4090 3748 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2649 3264 3384 4815 4850 5003 5201 6043 Size: 1296 615 120 1431 35 153 198 842 Cuts at: 6043 1353 Size: 3423 Fragments arranged by size: 3423 1431 1296 842 615 198 153 120 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 6700 7074 7220 387 Size: 1064 138 2663 2448 374 146 1280 Fragments arranged by size: 2663 2448 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2659 5124 2659 Size: 2465 5648 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2673 3517 3889 4136 4343 4430 4570 Size: 393 1041 844 372 247 207 87 140 Cuts at: 4570 4577 4610 4995 5182 6176 6773 7560 7828 Size: 7 33 385 187 994 597 787 268 Cuts at: 7828 1239 Size: 1524 Fragments arranged by size: 1524 1041 994 844 787 597 393 385 372 268 247 207 187 140 87 33 7 EcoNI CCTnn'n_nnAGG Cuts at: 3711 4216 5159 3711 Size: 505 943 6665 Fragments arranged by size: 6665 943 505 EcoO109I rG'GnC_Cy Cuts at: 4000 4380 4000 Size: 380 7733 EcoRI G'AATT_C Cuts at: 5422 5800 5422 Size: 378 7735 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2134 2444 2134 Size: 310 7803 FspI TGC'GCA Cuts at: 4230 6619 4230 Size: 2389 5724 HaeII r_GCGC'y Cuts at: 1678 2119 4339 7260 1678 Size: 441 2220 2921 2531 Fragments arranged by size: 2921 2531 2220 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 5051 5083 6754 Size: 32 135 32 905 32 2542 32 1671 Cuts at: 6754 6786 6828 6860 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2542 1671 905 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 3586 5457 5703 669 Size: 988 281 232 1416 1871 246 3079 Fragments arranged by size: 3079 1871 1416 988 281 246 232 HindIII A'AGCT_T Cuts at: 748 2360 2454 2675 748 Size: 1612 94 221 6186 Fragments arranged by size: 6186 1612 221 94 HpaI GTT'AAC Cuts at: 2170 5703 2170 Size: 3533 4580 KpnI G_GTAC'C Cuts at: 3859 4246 5438 3859 Size: 387 1192 6534 Fragments arranged by size: 6534 1192 387 MfeI C'AATT_G Cuts at: 5712 5712 Size: 8113 MluI A'CGCG_T Cuts at: 7989 7989 Size: 8113 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 4274 7227 7411 1305 Size: 1158 1811 2953 184 2007 Fragments arranged by size: 2953 2007 1811 1158 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3377 3740 4127 5225 Size: 54 1425 343 177 1368 363 387 1098 Cuts at: 5225 10 Size: 2898 Fragments arranged by size: 2898 1425 1368 1098 387 363 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 6071 6430 6589 518 Size: 648 2580 2325 359 159 2042 Fragments arranged by size: 2580 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2384 2635 3528 5529 6206 7354 7599 1606 Size: 778 251 893 2001 677 1148 245 2120 Fragments arranged by size: 2120 2001 1148 893 778 677 251 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6622 Fragments arranged by size: 6622 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8113 NotI GC'GGCC_GC Cuts at: 5560 5560 Size: 8113 NspI r_CATG'y Cuts at: 1132 1229 4436 4523 5373 5471 7016 1132 Size: 97 3207 87 850 98 1545 2229 Fragments arranged by size: 3207 2229 1545 850 98 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8113 PciI A'CATG_T Cuts at: 5369 7012 5369 Size: 1643 6470 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8113 PfoI T'CCnGG_A Cuts at: 2448 2448 Size: 8113 PmeI GTTT'AAAC Cuts at: 5417 5417 Size: 8113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6182 6214 7695 7727 6182 Size: 32 1481 32 6568 Fragments arranged by size: 6568 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4000 4380 4000 Size: 380 7733 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8113 PsiI TTA'TAA Cuts at: 5683 5683 Size: 8113 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2415 2447 2415 Size: 32 8081 PstI C_TGCA'G Cuts at: 830 5465 830 Size: 4635 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8113 PvuII CAG'CTG Cuts at: 2384 2635 3528 2384 Size: 251 893 6969 Fragments arranged by size: 6969 893 251 SacI G_AGCT'C Cuts at: 2661 5126 2661 Size: 2465 5648 SalI G'TCGA_C Cuts at: 1936 5455 1936 Size: 3519 4594 SanDI GG'GwC_CC Cuts at: 4380 4380 Size: 8113 SapI GCTCTTCn'nnn_ Cuts at: 2649 3264 2649 Size: 615 7498 SbfI CC_TGCA'GG Cuts at: 5465 5465 Size: 8113 ScaI AGT'ACT Cuts at: 6361 8073 6361 Size: 1712 6401 SexAI A'CCwGG_T Cuts at: 3567 3567 Size: 8113 SfcI C'TryA_G Cuts at: 826 1045 3073 3708 3758 5461 6596 7277 7468 Size: 219 2028 635 50 1703 1135 681 191 Cuts at: 7468 826 Size: 1471 Fragments arranged by size: 2028 1703 1471 1135 681 635 219 191 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8113 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8113 SmaI CCC'GGG Cuts at: 1872 5440 1872 Size: 3568 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2672 2678 2874 4705 6217 Size: 197 926 165 564 6 196 1831 1512 Cuts at: 6217 7118 7380 820 Size: 901 262 1553 Fragments arranged by size: 1831 1553 1512 926 901 564 262 197 196 165 6 SnaBI TAC'GTA Cuts at: 493 4565 5904 493 Size: 4072 1339 2702 Fragments arranged by size: 4072 2702 1339 SpeI A'CTAG_T Cuts at: 152 152 Size: 8113 SphI G_CATG'C Cuts at: 1132 4523 5471 1132 Size: 3391 948 3774 Fragments arranged by size: 3774 3391 948 SspI AAT'ATT Cuts at: 5 52 5275 6037 5 Size: 47 5223 762 2081 Fragments arranged by size: 5223 2081 762 47 StuI AGG'CCT Cuts at: 1517 3703 3914 1517 Size: 2186 211 5716 Fragments arranged by size: 5716 2186 211 StyI C'CwwG_G Cuts at: 513 1065 2004 4894 5109 5552 513 Size: 552 939 2890 215 443 3074 Fragments arranged by size: 3074 2890 939 552 443 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3143 3473 6140 6157 6310 6495 6940 Size: 1255 1563 330 2667 17 153 185 445 Cuts at: 6940 325 Size: 1498 Fragments arranged by size: 2667 1563 1498 1255 445 330 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5012 6359 8071 Size: 274 80 33 51 1443 3035 1347 1712 Cuts at: 8071 96 Size: 138 Fragments arranged by size: 3035 1712 1443 1347 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3377 3409 8073 8105 3377 Size: 32 4664 32 3385 Fragments arranged by size: 4664 3385 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8113 XbaI T'CTAG_A Cuts at: 2495 2887 2962 5449 2495 Size: 392 75 2487 5159 Fragments arranged by size: 5159 2487 392 75 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2446 2782 3548 3991 2011 Size: 435 336 766 443 6133 Fragments arranged by size: 6133 766 443 435 336 XhoI C'TCGA_G Cuts at: 1943 2874 1943 Size: 931 7182 XmaI C'CCGG_G Cuts at: 1870 5438 1870 Size: 3568 4545 XmnI GAAnn'nnTTC Cuts at: 4583 6242 4583 Size: 1659 6454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5921 276 Size: 53 83 186 936 4387 2468 Fragments arranged by size: 4387 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SfiI SfoI SrfI SwaI