(Circular) (Six-base) MAPSORT of: pFN21AB9559.seq Check: 8610 from: 1 to: 7963 pFN21AB9559 7963 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 5773 278 Size: 53 83 186 936 2290 1947 2468 Fragments arranged by size: 2468 2290 1947 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3949 4219 5306 1937 Size: 2012 270 1087 4594 Fragments arranged by size: 4594 2012 1087 270 Acc65I G'GTAC_C Cuts at: 4262 5284 4262 Size: 1022 6941 AclI AA'CG_TT Cuts at: 1563 2977 6090 6463 1563 Size: 1414 3113 373 3063 Fragments arranged by size: 3113 3063 1414 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 4628 6026 7410 7678 1632 Size: 845 2151 1398 1384 268 1917 Fragments arranged by size: 2151 1917 1398 1384 845 268 AfeI AGC'GCT Cuts at: 3389 3389 Size: 7963 AflII C'TTAA_G Cuts at: 820 1017 2050 820 Size: 197 1033 6733 Fragments arranged by size: 6733 1033 197 AflIII A'CryG_T Cuts at: 1203 2423 3741 4938 6862 7839 1203 Size: 1220 1318 1197 1924 977 1327 Fragments arranged by size: 1924 1327 1318 1220 1197 977 AgeI A'CCGG_T Cuts at: 2186 6794 2186 Size: 4608 3355 AhdI GACnn_n'nnGTC Cuts at: 6692 6692 Size: 7963 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7963 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3918 3950 4530 4562 4632 4664 3918 Size: 32 580 32 70 32 7217 Fragments arranged by size: 7217 580 70 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2864 4714 4828 5706 7278 2864 Size: 1850 114 878 1572 3549 Fragments arranged by size: 3549 1850 1572 878 114 ApaI G_GGCC'C Cuts at: 3446 3446 Size: 7963 ApaLI G'TGCA_C Cuts at: 6020 7176 6020 Size: 1156 6807 ApoI r'AATT_y Cuts at: 1493 2355 3074 4025 4161 4915 5033 5084 5272 Size: 862 719 951 136 754 118 51 188 Cuts at: 5272 5498 5650 7759 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 862 754 719 226 188 152 136 118 51 AseI AT'TA_AT Cuts at: 160 1032 2088 2730 6517 160 Size: 872 1056 642 3787 1606 Fragments arranged by size: 3787 1606 1056 872 642 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7963 AvaI C'yCGr_G Cuts at: 1870 1943 3662 5024 5288 1870 Size: 73 1719 1362 264 4545 Fragments arranged by size: 4545 1719 1362 264 73 BamHI G'GATC_C Cuts at: 2407 5293 5660 2407 Size: 2886 367 4710 Fragments arranged by size: 4710 2886 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4262 4421 5284 6739 618 Size: 325 217 627 2475 159 863 1455 1842 Fragments arranged by size: 2475 1842 1455 863 627 325 217 159 BanII G_rGCy'C Cuts at: 1391 2696 3446 5282 1391 Size: 1305 750 1836 4072 Fragments arranged by size: 4072 1836 1305 750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 2420 3858 4941 928 Size: 971 132 389 1438 1083 3950 Fragments arranged by size: 3950 1438 1083 971 389 132 BbvCI CC'TCA_GC Cuts at: 3573 4875 3573 Size: 1302 6661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6154 6188 1903 Size: 34 4217 34 3678 Fragments arranged by size: 4217 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5011 5855 7065 5011 Size: 844 1210 5909 Fragments arranged by size: 5909 1210 844 BclI T'GATC_A Cuts at: 1548 4273 1548 Size: 2725 5238 BfrBI ATG'CAT Cuts at: 2346 3817 2346 Size: 1471 6492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3646 6574 136 Size: 107 122 71 631 605 1974 2928 1525 Fragments arranged by size: 2928 1974 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5050 7958 5050 Size: 2908 5055 BlpI GC'TnA_GC Cuts at: 5380 5380 Size: 7963 Bme1580I G_kGCm'C Cuts at: 1790 3446 6024 7180 1790 Size: 1656 2578 1156 2573 Fragments arranged by size: 2578 2573 1656 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4115 5694 6652 Size: 416 53 83 399 24 2698 1579 958 Cuts at: 6652 442 Size: 1753 Fragments arranged by size: 2698 1753 1579 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4200 1056 Size: 3144 4819 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2375 2375 Size: 7963 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2537 3147 3987 4850 6623 1239 Size: 1106 192 610 840 863 1773 2579 Fragments arranged by size: 2579 1773 1106 863 840 610 192 Bpu10I CC'TnA_GC Cuts at: 3129 3573 4875 3129 Size: 444 1302 6217 Fragments arranged by size: 6217 1302 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3004 6088 6953 7251 3004 Size: 3084 865 298 3716 Fragments arranged by size: 3716 3084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2195 2584 6626 882 Size: 617 696 389 4042 2219 Fragments arranged by size: 4042 2219 696 617 389 BsaAI yAC'GTr Cuts at: 493 1204 5754 493 Size: 711 4550 2702 Fragments arranged by size: 4550 2702 711 BsaBI GATnn'nnATC Cuts at: 7957 7957 Size: 7963 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4765 5770 6152 Size: 53 83 186 936 2290 942 1005 382 Cuts at: 6152 6787 275 Size: 635 1451 Fragments arranged by size: 2290 1451 1005 942 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2186 6396 6794 7068 7215 7626 2186 Size: 4210 398 274 147 411 2523 Fragments arranged by size: 4210 2523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2502 2532 3523 3553 3918 Size: 30 165 30 1099 30 991 30 365 Cuts at: 3918 3948 3960 3990 1178 Size: 30 12 30 5151 Fragments arranged by size: 5151 1099 991 365 165 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3531 3939 5145 1186 Size: 2345 408 1206 4004 Fragments arranged by size: 4004 2345 1206 408 BseYI C'CCAG_C Cuts at: 2258 3604 4213 4859 7166 7662 2258 Size: 1346 609 646 2307 496 2559 Fragments arranged by size: 2559 2307 1346 646 609 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4356 4418 1163 Size: 573 2620 62 4708 Fragments arranged by size: 4708 2620 573 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5413 6174 7202 1540 Size: 275 186 3412 761 1028 2301 Fragments arranged by size: 3412 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 2696 2825 4099 6024 6109 7180 2331 Size: 365 129 1274 1925 85 1071 3114 Fragments arranged by size: 3114 1925 1274 1071 365 129 85 BsmI GAATG_Cn' Cuts at: 3684 3787 5474 5567 3684 Size: 103 1687 93 6080 Fragments arranged by size: 6080 1687 103 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2035 2035 Size: 7963 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2696 2825 3446 4099 5282 6024 Size: 399 541 365 129 621 653 1183 742 Cuts at: 6024 6109 7180 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1183 1071 742 653 621 541 399 365 129 85 BspEI T'CCGG_A Cuts at: 7626 7626 Size: 7963 BspHI T'CATG_A Cuts at: 5852 7582 5852 Size: 1730 6233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5318 844 Size: 4474 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5850 1394 Size: 429 4027 3507 Fragments arranged by size: 4027 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2159 6458 6632 65 Size: 1183 911 4299 174 1396 Fragments arranged by size: 4299 1396 1183 911 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 3870 6607 6794 1700 Size: 110 376 1684 2737 187 2869 Fragments arranged by size: 2869 2737 1684 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7963 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7963 BssSI C'ACGA_G Cuts at: 6023 7035 6023 Size: 1012 6951 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7963 BstBI TT'CG_AA Cuts at: 6767 6767 Size: 7963 BstEII G'GTnAC_C Cuts at: 1184 2216 1184 Size: 1032 6931 BstXI CCAn_nnnn'nTGG Cuts at: 4213 5011 4213 Size: 798 7165 BstYI r'GATC_y Cuts at: 1972 2232 2407 2785 3013 3151 4647 5050 5293 Size: 260 175 378 228 138 1496 403 243 Cuts at: 5293 5660 6045 6062 7514 7600 7612 7958 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1496 1452 403 385 378 367 346 260 243 228 175 138 86 17 12 BstZ17I GTA'TAC Cuts at: 4220 4220 Size: 7963 Bsu36I CC'TnA_GG Cuts at: 2545 2545 Size: 7963 BtgI C'CryG_G Cuts at: 513 1065 2004 4629 4651 513 Size: 552 939 2625 22 3825 Fragments arranged by size: 3825 2625 939 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3316 487 Size: 683 56 2090 5134 Fragments arranged by size: 5134 2090 683 56 BtsI GCAGTG_nn' Cuts at: 2511 5479 6273 6293 2511 Size: 2968 794 20 4181 Fragments arranged by size: 4181 2968 794 20 ClaI AT'CG_AT Cuts at: 1554 4773 1554 Size: 3219 4744 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7928 DraI TTT'AAA Cuts at: 2974 5267 2974 Size: 2293 5670 DraIII CAC_nnn'GTG Cuts at: 4697 4697 Size: 7963 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2639 5063 6970 809 Size: 507 1323 2424 1907 1802 Fragments arranged by size: 2424 1907 1802 1323 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 5410 6299 Size: 54 1425 50 275 18 1456 2124 889 Cuts at: 6299 7928 8 Size: 1629 43 Fragments arranged by size: 2124 1629 1456 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5410 1537 Size: 275 3598 4090 Fragments arranged by size: 4090 3598 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4008 4291 5893 1353 Size: 2655 283 1602 3423 Fragments arranged by size: 3423 2655 1602 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4671 6550 6924 7070 387 Size: 1064 138 3082 1879 374 146 1280 Fragments arranged by size: 3082 1879 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2694 2694 Size: 7963 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2477 2537 3147 3987 4628 4850 Size: 393 713 132 60 610 840 641 222 Cuts at: 4850 6026 6623 7410 7678 1239 Size: 1176 597 787 268 1524 Fragments arranged by size: 1524 1176 840 787 713 641 610 597 393 268 222 132 60 EcoO109I rG'GnC_Cy Cuts at: 2262 2313 3442 4238 2262 Size: 51 1129 796 5987 Fragments arranged by size: 5987 1129 796 51 EcoRI G'AATT_C Cuts at: 2355 5272 5650 2355 Size: 2917 378 4668 Fragments arranged by size: 4668 2917 378 EcoRV GAT'ATC Cuts at: 3191 3191 Size: 7963 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4687 4687 Size: 7963 FspI TGC'GCA Cuts at: 6469 6469 Size: 7963 HaeII r_GCGC'y Cuts at: 1678 3391 7110 1678 Size: 1713 3719 2531 Fragments arranged by size: 3719 2531 1713 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 3556 3588 6604 Size: 32 135 32 992 32 960 32 3016 Cuts at: 6604 6636 6678 6710 1373 Size: 32 42 32 2626 Fragments arranged by size: 3016 2626 992 960 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2157 4126 5307 5553 669 Size: 988 281 219 1969 1181 246 3079 Fragments arranged by size: 3079 1969 1181 988 281 246 219 HindIII A'AGCT_T Cuts at: 748 3591 748 Size: 2843 5120 HpaI GTT'AAC Cuts at: 5553 5553 Size: 7963 KpnI G_GTAC'C Cuts at: 4266 5288 4266 Size: 1022 6941 MfeI C'AATT_G Cuts at: 5562 5562 Size: 7963 MluI A'CGCG_T Cuts at: 7839 7839 Size: 7963 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2109 2204 7077 7261 1305 Size: 804 95 4873 184 2007 Fragments arranged by size: 4873 2007 804 184 95 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6141 Fragments arranged by size: 6141 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5921 6280 6439 518 Size: 648 4755 359 159 2042 Fragments arranged by size: 4755 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3041 3224 3604 4687 5379 6056 7204 7449 Size: 1435 183 380 1083 692 677 1148 245 Cuts at: 7449 1606 Size: 2120 Fragments arranged by size: 2120 1435 1148 1083 692 677 380 245 183 NcoI C'CATG_G Cuts at: 513 1065 2004 4629 513 Size: 552 939 2625 3847 Fragments arranged by size: 3847 2625 939 552 NdeI CA'TA_TG Cuts at: 387 3520 387 Size: 3133 4830 NheI G'CTAG_C Cuts at: 1052 4196 1052 Size: 3144 4819 NotI GC'GGCC_GC Cuts at: 5410 5410 Size: 7963 NsiI A_TGCA'T Cuts at: 2348 3819 2348 Size: 1471 6492 NspI r_CATG'y Cuts at: 1132 1229 2211 2427 3745 4597 5181 5321 6866 Size: 97 982 216 1318 852 584 140 1545 Cuts at: 6866 1132 Size: 2229 Fragments arranged by size: 2229 1545 1318 982 852 584 216 140 97 PasI CC'CwG_GG Cuts at: 1348 5161 1348 Size: 3813 4150 PciI A'CATG_T Cuts at: 2423 3741 6862 2423 Size: 1318 3121 3524 Fragments arranged by size: 3524 3121 1318 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 4630 1108 Size: 1215 2307 4441 Fragments arranged by size: 4441 2307 1215 PfoI T'CCnGG_A Cuts at: 2235 3846 2235 Size: 1611 6352 PmeI GTTT'AAAC Cuts at: 5267 5267 Size: 7963 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3918 3950 6032 6064 7545 7577 3918 Size: 32 2082 32 1481 32 4304 Fragments arranged by size: 4304 2082 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2313 2313 Size: 7963 PshAI GACnn'nnGTC Cuts at: 1613 5250 1613 Size: 3637 4326 PsiI TTA'TAA Cuts at: 4494 5533 4494 Size: 1039 6924 PspOMI G'GGCC_C Cuts at: 3442 3442 Size: 7963 PstI C_TGCA'G Cuts at: 830 3792 4731 5315 830 Size: 2962 939 584 3478 Fragments arranged by size: 3478 2962 939 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7963 PvuII CAG'CTG Cuts at: 3041 3224 3604 4687 3041 Size: 183 380 1083 6317 Fragments arranged by size: 6317 1083 380 183 SacI G_AGCT'C Cuts at: 2696 2696 Size: 7963 SalI G'TCGA_C Cuts at: 1936 5305 1936 Size: 3369 4594 SapI GCTCTTCn'nnn_ Cuts at: 4008 4291 4008 Size: 283 7680 SbfI CC_TGCA'GG Cuts at: 5315 5315 Size: 7963 ScaI AGT'ACT Cuts at: 3168 3209 6211 7923 3168 Size: 41 3002 1712 3208 Fragments arranged by size: 3208 3002 1712 41 SfcI C'TryA_G Cuts at: 826 1045 3788 3950 4291 4727 5311 6446 7127 Size: 219 2743 162 341 436 584 1135 681 Cuts at: 7127 7318 826 Size: 191 1471 Fragments arranged by size: 2743 1471 1135 681 584 436 341 219 191 162 SfiI GGCCn_nnn'nGGCC Cuts at: 3646 3646 Size: 7963 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7963 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7963 SmaI CCC'GGG Cuts at: 1872 5290 1872 Size: 3418 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2050 2983 6067 6968 7230 820 Size: 197 926 107 933 3084 901 262 1553 Fragments arranged by size: 3084 1553 933 926 901 262 197 107 SnaBI TAC'GTA Cuts at: 493 5754 493 Size: 5261 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7963 SphI G_CATG'C Cuts at: 1132 4597 5321 1132 Size: 3465 724 3774 Fragments arranged by size: 3774 3465 724 SspI AAT'ATT Cuts at: 5 52 3806 4951 5887 5 Size: 47 3754 1145 936 2081 Fragments arranged by size: 3754 2081 1145 936 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7963 StyI C'CwwG_G Cuts at: 513 1065 2004 4629 4671 5402 513 Size: 552 939 2625 42 731 3074 Fragments arranged by size: 3074 2625 939 731 552 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4409 4520 4827 5990 6007 6160 Size: 1255 2589 240 111 307 1163 17 153 Cuts at: 6160 6345 6790 325 Size: 185 445 1498 Fragments arranged by size: 2589 1498 1255 1163 445 307 240 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3136 3166 3207 Size: 274 80 33 51 1443 1159 30 41 Cuts at: 3207 4965 5008 6209 7921 96 Size: 1758 43 1201 1712 138 Fragments arranged by size: 1758 1712 1443 1201 1159 274 138 80 51 43 41 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 3684 3716 7923 7955 2502 Size: 32 1150 32 4207 32 2510 Fragments arranged by size: 4207 2510 1150 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7963 XbaI T'CTAG_A Cuts at: 2719 5053 5299 2719 Size: 2334 246 5383 Fragments arranged by size: 5383 2334 246 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7963 XmaI C'CCGG_G Cuts at: 1870 5288 1870 Size: 3418 4545 XmnI GAAnn'nnTTC Cuts at: 3558 4017 4164 6092 3558 Size: 459 147 1928 5429 Fragments arranged by size: 5429 1928 459 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 5771 276 Size: 53 83 186 936 2290 1947 2468 Fragments arranged by size: 2468 2290 1947 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BsiWI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI