Restriction map for
pFN21AB9557
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbrPI BcgI BfrI BglII BsaAI
BsaWI BseAI BsiEI BsiWI BspEI
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
FspI HpaI KpnI KspI MluI
MroI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacI SacII SbfI ScaI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 929 GTCGAC GT^[AC][GT]AC
AccII 5665 CGCG CG^CG
AspHI 4671 GAGCAC G[AT]GC[AT]^C
AspI 3497 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 934 CCTAGG C^CTAGG
BamHI 4549 GGATCC G^GATCC
BbeI 4321 GGCGCC GGCGC^C
BciVI 5844 GTATCC GTATCC(6/5)
BclI 5147 TGATCA T^GATCA
BcnI 2244 CCGGG CC^[GC]GG
BglI 5647 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 934 CCTAGG C^CTAGG
BlpI 5374 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5374 GCTCAGC GC^T[ACGT]AGC
BsaBI 2302 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 4671 GAGCAC G[AT]GC[AT]^C
BsmBI 2668 GAGACG CGTCTC(1/5)
BspLU11I 5492 ACATGT A^CATGT
BssSI 4008 CACGAG CACGAG(-5/-1)
BstBI 1067 TTCGAA TT^CGAA
BstUI 5665 CGCG CG^CG
CelII 5374 GCTCAGC GC^T[ACGT]AGC
DrdI 2929 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3654 CCTACCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3402 GAATTC G^AATTC
EcoRV 1459 GATATC GAT^ATC
FbaI 5147 TGATCA T^GATCA
HindIII 2275 AAGCTT A^AGCTT
KasI 4321 GGCGCC G^GCGCC
MamI 2302 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 148 CAATTG C^AATTG
MunI 148 CAATTG C^AATTG
MvnI 5665 CGCG CG^CG
NaeI 5221 GCCGGC GCC^GGC
NarI 4321 GGCGCC GG^CGCC
NciI 2244 CCGGG CC^[GC]GG
NgoMIV 5221 GCCGGC G^CCGGC
NspV 1067 TTCGAA TT^CGAA
PflFI 3497 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2328 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 929 GTCGAC G^TCGAC
SfoI 4321 GGCGCC GGC^GCC
SfuI 1067 TTCGAA TT^CGAA
SphI 3556 GCATGC GCATG^C
Tth111I 3497 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AatII 1007 GACGTC GACGT^C
2670 GACGTC
AclI 365 AACGTT AA^CGTT
2630 AACGTT
Asp700I 1447 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4237 GAAGAGATTC
AvaI 1277 CTCGGG C^[CT]CG[GA]G
3658 CCCGAG
BanI 4321 GGCGCC G^G[CT][GA]CC
5218 GGTGCC
BbvCI 5053 CCTCAGC CCTCAGC(-5/-2)
2380 GCTGAGG
BsgI 2877 GTGCAG GTGCAG(16/14)
4282 GTGCAG
BsoBI 1277 CTCGGG C^[CT]CG[GA]G
3658 CCCGAG
Bsp1407I 4272 TGTACA T^GTACA
5930 TGTACA
BspDI 1987 ATCGAT AT^CGAT
3199 ATCGAT
BspMI 1976 GCAGGT ACCTGC(4/8)
4629 GCAGGT
BsrBI 5571 CCGCTC CCGCTC(-3/-3)
1153 GAGCGG
BsrDI 98 GCAATG GCAATG(2/0)
1187 GCAATG
BsrFI 600 GCCGGT [GA]^CCGG[CT]
5221 GCCGGC
BsrGI 4272 TGTACA T^GTACA
5930 TGTACA
Cfr10I 600 GCCGGT [GA]^CCGG[CT]
5221 GCCGGC
ClaI 1987 ATCGAT AT^CGAT
3199 ATCGAT
DraI 180 TTTAAA TTT^AAA
723 TTTAAA
HaeII 986 GGCGCT [GA]GCGC^[CT]
4321 GGCGCC
HincII 929 GTCGAC GT[CT]^[GA]AC
2154 GTTGAC
HindII 929 GTCGAC GT[CT]^[GA]AC
2154 GTTGAC
PflMI 117 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5161 CCAGAATCTGG
PpuMI 1050 AGGTCCC [GA]G^G[AT]CC[CT]
4391 AGGACCC
Psp1406I 365 AACGTT AA^CGTT
2630 AACGTT
SapI 2398 GAAGAGC GCTCTTC(1/4)
4742 GCTCTTC
SexAI 1561 ACCTGGT A^CC[AT]GGT
3498 ACCTGGT
SspBI 4272 TGTACA T^GTACA
5930 TGTACA
SspI 1723 AATATT AAT^ATT
2094 AATATT
Van91I 117 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5161 CCAGAATCTGG
XmnI 1447 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4237 GAAGAGATTC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII