(Circular) (Six-base) MAPSORT of: pFN21AB9557.seq Check: 3952 from: 1 to: 10717 pFN21AB9557 10717 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6625 6625 Size: 10717 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3016 4679 8527 278 Size: 53 83 186 936 1480 1663 3848 2468 Fragments arranged by size: 3848 2468 1663 1480 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2935 8060 1937 Size: 998 5125 4594 Fragments arranged by size: 5125 4594 998 Acc65I G'GTAC_C Cuts at: 8038 8038 Size: 10717 AclI AA'CG_TT Cuts at: 1563 2371 4636 8844 9217 1563 Size: 808 2265 4208 373 3063 Fragments arranged by size: 4208 3063 2265 808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2070 3046 3336 4268 4665 4898 4939 5054 Size: 438 976 290 932 397 233 41 115 Cuts at: 5054 5151 5733 6637 7240 7472 8780 10164 10432 Size: 97 582 904 603 232 1308 1384 268 Cuts at: 10432 1632 Size: 1917 Fragments arranged by size: 1917 1384 1308 976 932 904 603 582 438 397 290 268 233 232 115 97 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10520 AflIII A'CryG_T Cuts at: 1203 4535 6147 7497 9616 10593 1203 Size: 3332 1612 1350 2119 977 1327 Fragments arranged by size: 3332 2119 1612 1350 1327 977 AgeI A'CCGG_T Cuts at: 9548 9548 Size: 10717 AhdI GACnn_n'nnGTC Cuts at: 9446 9446 Size: 10717 AleI CACnn'nnGTG Cuts at: 1166 5132 6152 1166 Size: 3966 1020 5731 Fragments arranged by size: 5731 3966 1020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6875 1184 Size: 5691 5026 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6734 6766 6734 Size: 32 10685 AlwNI CAG_nnn'CTG Cuts at: 2342 2805 5927 6370 6735 6849 7172 7279 7624 Size: 463 3122 443 365 114 323 107 345 Cuts at: 7624 8460 10032 2342 Size: 836 1572 3027 Fragments arranged by size: 3122 3027 1572 836 463 443 365 345 323 114 107 ApaLI G'TGCA_C Cuts at: 8774 9930 8774 Size: 1156 9561 ApoI r'AATT_y Cuts at: 1493 3248 3558 3626 4291 5407 8026 8252 8404 Size: 1755 310 68 665 1116 2619 226 152 Cuts at: 8404 10513 1493 Size: 2109 1697 Fragments arranged by size: 2619 2109 1755 1697 1116 665 310 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 9271 160 Size: 872 8239 1606 Fragments arranged by size: 8239 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10717 AvaI C'yCGr_G Cuts at: 1870 1943 3282 5663 8042 1870 Size: 73 1339 2381 2379 4545 Fragments arranged by size: 4545 2381 2379 1339 73 AvrII C'CTAG_G Cuts at: 2939 2939 Size: 10717 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7177 7210 7177 Size: 33 10684 BamHI G'GATC_C Cuts at: 6554 8047 8414 6554 Size: 1493 367 8857 Fragments arranged by size: 8857 1493 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6326 7223 8038 9493 618 Size: 325 217 627 4539 897 815 1455 1842 Fragments arranged by size: 4539 1842 1455 897 815 627 325 217 BanII G_rGCy'C Cuts at: 1391 3231 4842 5577 8036 1391 Size: 1840 1611 735 2459 4072 Fragments arranged by size: 4072 2459 1840 1611 735 BbeI G_GCGC'C Cuts at: 6330 6330 Size: 10717 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 4562 4671 4825 5262 5655 6204 Size: 971 207 2456 109 154 437 393 549 Cuts at: 6204 6426 928 Size: 222 5219 Fragments arranged by size: 5219 2456 971 549 437 393 222 207 154 109 BbvCI CC'TCA_GC Cuts at: 4386 7059 4386 Size: 2673 8044 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8908 8942 1903 Size: 34 6971 34 3678 Fragments arranged by size: 6971 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7860 8609 9819 7860 Size: 749 1210 8758 Fragments arranged by size: 8758 1210 749 BclI T'GATC_A Cuts at: 1548 7152 1548 Size: 5604 5113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7658 9328 136 Size: 107 122 71 631 605 5986 1670 1525 Fragments arranged by size: 5986 1670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 10712 10712 Size: 10717 BlpI GC'TnA_GC Cuts at: 7380 8134 7380 Size: 754 9963 Bme1580I G_kGCm'C Cuts at: 1790 7566 7983 8778 9934 1790 Size: 5776 417 795 1156 2573 Fragments arranged by size: 5776 2573 1156 795 417 BmgBI CAC'GTC Cuts at: 1318 6150 1318 Size: 4832 5885 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6930 7191 8448 Size: 416 53 83 399 24 5513 261 1257 Cuts at: 8448 9406 442 Size: 958 1753 Fragments arranged by size: 5513 1753 1257 958 416 399 261 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4871 4871 Size: 10717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 4023 4320 4506 5748 5996 6772 9377 Size: 1317 1467 297 186 1242 248 776 2605 Cuts at: 9377 1239 Size: 2579 Fragments arranged by size: 2605 2579 1467 1317 1242 776 297 248 186 Bpu10I CC'TnA_GC Cuts at: 2253 4386 7059 7402 2253 Size: 2133 2673 343 5568 Fragments arranged by size: 5568 2673 2133 343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3573 3583 4161 4841 5438 5541 6285 6539 7401 Size: 10 578 680 597 103 744 254 862 Cuts at: 7401 8842 9707 10005 3573 Size: 1441 865 298 4285 Fragments arranged by size: 4285 1441 865 862 744 680 597 578 298 254 103 10 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4041 4366 5117 5783 6581 9380 882 Size: 617 2542 325 751 666 798 2799 2219 Fragments arranged by size: 2799 2542 2219 798 751 666 617 325 BsaAI yAC'GTr Cuts at: 493 1204 8508 493 Size: 711 7304 2702 Fragments arranged by size: 7304 2702 711 BsaBI GATnn'nnATC Cuts at: 4311 10711 4311 Size: 6400 4317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3013 4676 6327 7436 Size: 53 83 186 936 1480 1663 1651 1109 Cuts at: 7436 8524 8906 9541 275 Size: 1088 382 635 1451 Fragments arranged by size: 1663 1651 1480 1451 1109 1088 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9150 9548 9822 9969 10380 9150 Size: 398 274 147 411 9487 Fragments arranged by size: 9487 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5037 5067 7129 7159 1178 Size: 30 165 30 3634 30 2062 30 4736 Fragments arranged by size: 4736 3634 2062 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3419 3565 4382 4394 4677 4790 4985 4988 Size: 2233 146 817 12 283 113 195 3 Cuts at: 4988 4991 4994 5080 5231 5403 5681 5693 5696 Size: 3 3 86 151 172 278 12 3 Cuts at: 5696 5699 5831 5861 5879 5882 5900 5915 5918 Size: 3 132 30 18 3 18 15 3 Cuts at: 5918 6047 6143 6251 6445 7404 7470 1186 Size: 129 96 108 194 959 66 4433 Fragments arranged by size: 4433 2233 959 817 283 278 195 194 172 151 146 132 129 113 108 96 86 66 30 18 18 15 12 12 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3381 4605 5752 6173 6473 7029 7065 7092 7141 Size: 1224 1147 421 300 556 36 27 49 Cuts at: 7141 7406 7896 9920 10416 3381 Size: 265 490 2024 496 3682 Fragments arranged by size: 3682 2024 1224 1147 556 496 490 421 300 265 49 36 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4903 6308 1163 Size: 573 3167 1405 5572 Fragments arranged by size: 5572 3167 1405 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8167 8928 9956 1540 Size: 275 186 6166 761 1028 2301 Fragments arranged by size: 6166 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6680 8778 8863 9934 6680 Size: 2098 85 1071 7463 Fragments arranged by size: 7463 2098 1071 85 BsmI GAATG_Cn' Cuts at: 4215 7518 7768 8228 8321 4215 Size: 3303 250 460 93 6611 Fragments arranged by size: 6611 3303 460 250 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4667 4667 Size: 10717 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3231 4842 5577 6680 7566 7983 8036 Size: 399 1441 1611 735 1103 886 417 53 Cuts at: 8036 8778 8863 9934 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1611 1441 1103 1071 886 742 735 417 399 85 53 BspEI T'CCGG_A Cuts at: 10380 10380 Size: 10717 BspHI T'CATG_A Cuts at: 3432 4459 4561 8606 10336 3432 Size: 1027 102 4045 1730 3813 Fragments arranged by size: 4045 3813 1730 1027 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3972 6625 8072 844 Size: 3128 2653 1447 3489 Fragments arranged by size: 3489 3128 2653 1447 BsrBI CCG'CTC Cuts at: 1394 1823 3160 7578 8604 1394 Size: 429 1337 4418 1026 3507 Fragments arranged by size: 4418 3507 1337 1026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2110 3199 9212 9386 65 Size: 1183 862 1089 6013 174 1396 Fragments arranged by size: 6013 1396 1183 1089 862 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2605 7226 9361 9548 1700 Size: 110 795 4621 2135 187 2869 Fragments arranged by size: 4621 2869 2135 795 187 110 BsrGI T'GTAC_A Cuts at: 96 6277 7935 96 Size: 6181 1658 2878 Fragments arranged by size: 6181 2878 1658 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10717 BssSI C'ACGA_G Cuts at: 6013 8777 9789 6013 Size: 2764 1012 6941 Fragments arranged by size: 6941 2764 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5927 6849 7537 7906 794 Size: 5133 922 688 369 3605 Fragments arranged by size: 5133 3605 922 688 369 BstBI TT'CG_AA Cuts at: 3073 9521 3073 Size: 6448 4269 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10717 BstXI CCAn_nnnn'nTGG Cuts at: 3890 4357 5490 3890 Size: 467 1133 9117 Fragments arranged by size: 9117 1133 467 BstYI r'GATC_y Cuts at: 1972 2221 4312 5613 6265 6554 8047 8414 8799 Size: 249 2091 1301 652 289 1493 367 385 Cuts at: 8799 8816 10268 10354 10366 10712 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2091 1977 1493 1452 1301 652 385 367 346 289 249 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4689 4869 4946 5808 4689 Size: 180 77 862 9598 Fragments arranged by size: 9598 862 180 77 BtgI C'CryG_G Cuts at: 513 1065 2004 2122 2266 2473 6153 6411 513 Size: 552 939 118 144 207 3680 258 4819 Fragments arranged by size: 4819 3680 939 552 258 207 144 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9978 Fragments arranged by size: 9978 683 56 BtsI GCAGTG_nn' Cuts at: 2422 2783 3479 3913 5104 5749 6291 6319 7592 Size: 361 696 434 1191 645 542 28 1273 Cuts at: 7592 8233 9027 9047 2422 Size: 641 794 20 4092 Fragments arranged by size: 4092 1273 1191 794 696 645 641 542 434 361 28 20 ClaI AT'CG_AT Cuts at: 1554 3993 5205 1554 Size: 2439 1212 7066 Fragments arranged by size: 7066 2439 1212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2511 2546 7495 7530 529 Size: 35 1947 35 4949 35 3716 Fragments arranged by size: 4949 3716 1947 35 35 35 DraI TTT'AAA Cuts at: 2187 2730 8021 2187 Size: 543 5291 4883 Fragments arranged by size: 5291 4883 543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4940 9724 809 Size: 507 3624 4784 1802 Fragments arranged by size: 4784 3624 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2470 2713 5968 Size: 54 1425 50 275 18 640 243 3255 Cuts at: 5968 6831 7630 8164 9053 10682 8 Size: 863 799 534 889 1629 43 Fragments arranged by size: 3255 1629 1425 889 863 799 640 534 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8164 1537 Size: 275 6352 4090 Fragments arranged by size: 6352 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4371 4398 4665 5208 5217 5305 5331 5700 Size: 3018 27 267 543 9 88 26 369 Cuts at: 5700 5709 5721 5820 6071 6120 6210 6237 6754 Size: 9 12 99 251 49 90 27 517 Cuts at: 6754 8008 8647 1353 Size: 1254 639 3423 Fragments arranged by size: 3423 3018 1254 639 543 517 369 267 251 99 90 88 49 27 27 26 12 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2877 3289 4235 9304 9678 9824 Size: 1064 138 1288 412 946 5069 374 146 Cuts at: 9824 387 Size: 1280 Fragments arranged by size: 5069 1288 1280 1064 946 412 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2556 3046 3336 4023 4268 4320 Size: 393 438 486 490 290 687 245 52 Cuts at: 4320 4506 4665 4898 4939 5054 5151 5733 5748 Size: 186 159 233 41 115 97 582 15 Cuts at: 5748 5996 6637 6772 7240 7472 8780 9377 10164 Size: 248 641 135 468 232 1308 597 787 Cuts at: 10164 10432 1239 Size: 268 1524 Fragments arranged by size: 1524 1308 787 687 641 597 582 490 486 468 438 393 290 268 248 245 233 232 186 159 135 115 97 52 41 15 EcoNI CCTnn'n_nnAGG Cuts at: 5663 5663 Size: 10717 EcoO109I rG'GnC_Cy Cuts at: 3056 6397 7943 3056 Size: 3341 1546 5830 Fragments arranged by size: 5830 3341 1546 EcoRI G'AATT_C Cuts at: 5407 8026 8404 5407 Size: 2619 378 7720 Fragments arranged by size: 7720 2619 378 EcoRV GAT'ATC Cuts at: 3466 3466 Size: 10717 FspI TGC'GCA Cuts at: 9223 9223 Size: 10717 HaeII r_GCGC'y Cuts at: 1678 2995 6330 9864 1678 Size: 1317 3335 3534 2531 Fragments arranged by size: 3534 3335 2531 1317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2439 2471 3940 3972 5147 Size: 32 135 32 867 32 1469 32 1175 Cuts at: 5147 5179 5324 5356 7705 7737 9358 9390 9432 Size: 32 145 32 2349 32 1621 32 42 Cuts at: 9432 9464 1373 Size: 32 2626 Fragments arranged by size: 2626 2349 1621 1469 1175 867 145 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2936 4161 8061 8307 669 Size: 988 281 998 1225 3900 246 3079 Fragments arranged by size: 3900 3079 1225 998 988 281 246 HindIII A'AGCT_T Cuts at: 748 4280 748 Size: 3532 7185 HpaI GTT'AAC Cuts at: 8307 8307 Size: 10717 KasI G'GCGC_C Cuts at: 6326 6326 Size: 10717 KpnI G_GTAC'C Cuts at: 8042 8042 Size: 10717 MfeI C'AATT_G Cuts at: 2153 8316 2153 Size: 6163 4554 MluI A'CGCG_T Cuts at: 10593 10593 Size: 10717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3472 4374 9831 10015 1305 Size: 2167 902 5457 184 2007 Fragments arranged by size: 5457 2167 2007 902 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2472 2715 5970 6833 10 Size: 54 1425 343 640 243 3255 863 3894 Fragments arranged by size: 3894 3255 1425 863 640 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2900 4122 5132 6152 7292 7463 7697 Size: 648 1734 1222 1010 1020 1140 171 234 Cuts at: 7697 7748 8675 9034 9193 518 Size: 51 927 359 159 2042 Fragments arranged by size: 2042 1734 1222 1140 1020 1010 927 648 359 234 171 159 51 MspA1I CmG'CkG Cuts at: 1606 3525 4288 4605 4762 5918 6648 6762 6810 Size: 1919 763 317 157 1156 730 114 48 Cuts at: 6810 6849 7108 7279 7384 7624 8133 8810 9958 Size: 39 259 171 105 240 509 677 1148 Cuts at: 9958 10203 1606 Size: 245 2120 Fragments arranged by size: 2120 1919 1156 1148 763 730 677 509 317 259 245 240 171 157 114 105 48 39 NaeI GCC'GGC Cuts at: 7228 7228 Size: 10717 NarI GG'CG_CC Cuts at: 6327 6327 Size: 10717 NcoI C'CATG_G Cuts at: 513 1065 2004 2122 2266 6411 513 Size: 552 939 118 144 4145 4819 Fragments arranged by size: 4819 4145 939 552 144 118 NdeI CA'TA_TG Cuts at: 387 387 Size: 10717 NgoMIV G'CCGG_C Cuts at: 7226 7226 Size: 10717 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10717 NotI GC'GGCC_GC Cuts at: 8164 8164 Size: 10717 NspI r_CATG'y Cuts at: 1132 1229 2799 2839 2917 5565 5656 7501 8075 Size: 97 1570 40 78 2648 91 1845 574 Cuts at: 8075 9620 1132 Size: 1545 2229 Fragments arranged by size: 2648 2229 1845 1570 1545 574 97 91 78 40 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10717 PciI A'CATG_T Cuts at: 7497 9616 7497 Size: 2119 8598 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 7172 1108 Size: 1020 5044 4653 Fragments arranged by size: 5044 4653 1020 PfoI T'CCnGG_A Cuts at: 4263 5616 4263 Size: 1353 9364 PmeI GTTT'AAAC Cuts at: 8021 8021 Size: 10717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8786 8818 10299 10331 8786 Size: 32 1481 32 9172 Fragments arranged by size: 9172 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3056 6397 3056 Size: 3341 7376 PshAI GACnn'nnGTC Cuts at: 1613 4337 1613 Size: 2724 7993 PsiI TTA'TAA Cuts at: 8287 8287 Size: 10717 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7793 7825 7919 7951 7793 Size: 32 94 32 10559 Fragments arranged by size: 10559 94 32 32 PstI C_TGCA'G Cuts at: 830 3185 3486 3666 5923 6375 6854 6896 7275 Size: 2355 301 180 2257 452 479 42 379 Cuts at: 7275 8069 830 Size: 794 3478 Fragments arranged by size: 3478 2355 2257 794 479 452 379 301 180 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10717 PvuII CAG'CTG Cuts at: 4288 4762 5918 6762 6810 6849 7279 7384 7624 Size: 474 1156 844 48 39 430 105 240 Cuts at: 7624 4288 Size: 7381 Fragments arranged by size: 7381 1156 844 474 430 240 105 48 39 SalI G'TCGA_C Cuts at: 1936 2934 8059 1936 Size: 998 5125 4594 Fragments arranged by size: 5125 4594 998 SapI GCTCTTCn'nnn_ Cuts at: 4398 6754 4398 Size: 2356 8361 SbfI CC_TGCA'GG Cuts at: 8069 8069 Size: 10717 ScaI AGT'ACT Cuts at: 8965 10677 8965 Size: 1712 9005 SexAI A'CCwGG_T Cuts at: 3566 5503 3566 Size: 1937 8780 SfcI C'TryA_G Cuts at: 826 1045 2629 3181 3482 3662 4302 4921 5919 Size: 219 1584 552 301 180 640 619 998 Cuts at: 5919 6371 6850 6892 7271 8065 9200 9881 10072 Size: 452 479 42 379 794 1135 681 191 Cuts at: 10072 826 Size: 1471 Fragments arranged by size: 1584 1471 1135 998 794 681 640 619 552 479 452 379 301 219 191 180 42 SfoI GGC'GCC Cuts at: 6328 6328 Size: 10717 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10717 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10717 SmaI CCC'GGG Cuts at: 1872 8044 1872 Size: 6172 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3588 3598 4176 4856 5417 5556 Size: 197 926 1645 10 578 680 561 139 Cuts at: 5556 6300 6518 7416 8821 9722 9984 820 Size: 744 218 898 1405 901 262 1553 Fragments arranged by size: 1645 1553 1405 926 901 898 744 680 578 561 262 218 197 139 10 SnaBI TAC'GTA Cuts at: 493 8508 493 Size: 8015 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10717 SphI G_CATG'C Cuts at: 1132 5565 8075 1132 Size: 4433 2510 3774 Fragments arranged by size: 4433 3774 2510 SspI AAT'ATT Cuts at: 5 52 3730 4101 8641 5 Size: 47 3678 371 4540 2081 Fragments arranged by size: 4540 3678 2081 371 47 StuI AGG'CCT Cuts at: 1517 2765 6931 7889 1517 Size: 1248 4166 958 4345 Fragments arranged by size: 4345 4166 1248 958 StyI C'CwwG_G Cuts at: 513 1065 2004 2122 2266 2939 5799 6411 8156 Size: 552 939 118 144 673 2860 612 1745 Cuts at: 8156 513 Size: 3074 Fragments arranged by size: 3074 2860 1745 939 673 612 552 144 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2874 4745 4986 5807 6398 6481 6548 Size: 1255 1294 1871 241 821 591 83 67 Cuts at: 6548 6815 8744 8761 8914 9099 9544 325 Size: 267 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1871 1498 1294 1255 821 591 445 267 241 185 153 83 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3853 3927 5281 Size: 274 80 33 51 1443 1876 74 1354 Cuts at: 5281 6277 7935 8963 10675 96 Size: 996 1658 1028 1712 138 Fragments arranged by size: 1876 1712 1658 1443 1354 1028 996 274 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4437 4469 5963 5995 7129 7161 10677 10709 4437 Size: 32 1494 32 1134 32 3516 32 4445 Fragments arranged by size: 4445 3516 1494 1134 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5505 1316 Size: 4189 6528 XbaI T'CTAG_A Cuts at: 8053 8053 Size: 10717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2913 4637 6598 7291 7372 2913 Size: 1724 1961 693 81 6258 Fragments arranged by size: 6258 1961 1724 693 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10717 XmaI C'CCGG_G Cuts at: 1870 8042 1870 Size: 6172 4545 XmnI GAAnn'nnTTC Cuts at: 3456 6246 8846 3456 Size: 2790 2600 5327 Fragments arranged by size: 5327 2790 2600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3014 4677 8525 276 Size: 53 83 186 936 1480 1663 3848 2468 Fragments arranged by size: 3848 2468 1663 1480 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BfrBI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI