Restriction map for
pFN21AB9557
(Linear) (Six-base) MAPSORT of: KIBB9557.seq Check: 6569 from: 1 to: 6012
KIBB9557.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4620 6012
Size: 4620 1392
AatII G_ACGT'C
Cuts at: 0 1011 2674 6012
Size: 1011 1663 3338
Fragments arranged by size:
3338 1663 1011
AccI GT'mk_AC
Cuts at: 0 930 6012
Size: 930 5082
AclI AA'CG_TT
Cuts at: 0 366 2631 6012
Size: 366 2265 3381
Fragments arranged by size:
3381 2265 366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1041 1331 2263 2660 2893 2934 3049
Size: 65 976 290 932 397 233 41 115
Cuts at: 3049 3146 3728 4632 5235 5467 6012
Size: 97 582 904 603 232 545
Fragments arranged by size:
976 932 904 603 582 545 397 290
233 232 115 97 65 41
AflIII A'CryG_T
Cuts at: 0 2530 4142 5492 6012
Size: 2530 1612 1350 520
Fragments arranged by size:
2530 1612 1350 520
AleI CACnn'nnGTG
Cuts at: 0 3127 4147 6012
Size: 3127 1020 1865
Fragments arranged by size:
3127 1865 1020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4870 6012
Size: 4870 1142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4729 4761 6012
Size: 4729 32 1251
Fragments arranged by size:
4729 1251 32
AlwNI CAG_nnn'CTG
Cuts at: 0 337 800 3922 4365 4730 4844 5167 5274
Size: 337 463 3122 443 365 114 323 107
Cuts at: 5274 5619 6012
Size: 345 393
Fragments arranged by size:
3122 463 443 393 365 345 337 323
114 107
ApoI r'AATT_y
Cuts at: 0 1243 1553 1621 2286 3402 6012
Size: 1243 310 68 665 1116 2610
Fragments arranged by size:
2610 1243 1116 665 310 68
AvaI C'yCGr_G
Cuts at: 0 1277 3658 6012
Size: 1277 2381 2354
Fragments arranged by size:
2381 2354 1277
AvrII C'CTAG_G
Cuts at: 0 934 6012
Size: 934 5078
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5172 5205 6012
Size: 5172 33 807
Fragments arranged by size:
5172 807 33
BamHI G'GATC_C
Cuts at: 0 4549 6012
Size: 4549 1463
BanI G'GyrC_C
Cuts at: 0 4321 5218 6012
Size: 4321 897 794
Fragments arranged by size:
4321 897 794
BanII G_rGCy'C
Cuts at: 0 1226 2837 3572 6012
Size: 1226 1611 735 2440
Fragments arranged by size:
2440 1611 1226 735
BbeI G_GCGC'C
Cuts at: 0 4325 6012
Size: 4325 1687
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 2557 2666 2820 3257 3650 4199 4421
Size: 101 2456 109 154 437 393 549 222
Cuts at: 4421 6012
Size: 1591
Fragments arranged by size:
2456 1591 549 437 393 222 154 109
101
BbvCI CC'TCA_GC
Cuts at: 0 2381 5054 6012
Size: 2381 2673 958
Fragments arranged by size:
2673 2381 958
BciVI GTATCCnnnnn_n'
Cuts at: 0 5855 6012
Size: 5855 157
BclI T'GATC_A
Cuts at: 0 5147 6012
Size: 5147 865
BglI GCCn_nnn'nGGC
Cuts at: 0 5653 6012
Size: 5653 359
BlpI GC'TnA_GC
Cuts at: 0 5375 6012
Size: 5375 637
Bme1580I G_kGCm'C
Cuts at: 0 5561 5978 6012
Size: 5561 417 34
Fragments arranged by size:
5561 417 34
BmgBI CAC'GTC
Cuts at: 0 4145 6012
Size: 4145 1867
BmrI ACTGGGnnnn_n'
Cuts at: 0 4925 5186 6012
Size: 4925 261 826
Fragments arranged by size:
4925 826 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2866 6012
Size: 2866 3146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 2018 2315 2501 3743 3991 4767 6012
Size: 551 1467 297 186 1242 248 776 1245
Fragments arranged by size:
1467 1245 1242 776 551 297 248 186
Bpu10I CC'TnA_GC
Cuts at: 0 248 2381 5054 5397 6012
Size: 248 2133 2673 343 615
Fragments arranged by size:
2673 2133 615 343 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1578 2156 2836 3433 3536 4280 4534
Size: 1568 10 578 680 597 103 744 254
Cuts at: 4534 5396 6012
Size: 862 616
Fragments arranged by size:
1568 862 744 680 616 597 578 254
103 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 2036 2361 3112 3778 4576 6012
Size: 2036 325 751 666 798 1436
Fragments arranged by size:
2036 1436 798 751 666 325
BsaBI GATnn'nnATC
Cuts at: 0 2306 6012
Size: 2306 3706
BsaHI Gr'CG_yC
Cuts at: 0 1008 2671 4322 5431 6012
Size: 1008 1663 1651 1109 581
Fragments arranged by size:
1663 1651 1109 1008 581
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3032 3062 5124 5154 6012
Size: 3032 30 2062 30 858
Fragments arranged by size:
3032 2062 858 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1414 1560 2377 2389 2672 2785 2980 2983
Size: 1414 146 817 12 283 113 195 3
Cuts at: 2983 2986 2989 3075 3226 3398 3676 3688 3691
Size: 3 3 86 151 172 278 12 3
Cuts at: 3691 3694 3826 3856 3874 3877 3895 3910 3913
Size: 3 132 30 18 3 18 15 3
Cuts at: 3913 4042 4138 4246 4440 5399 5465 6012
Size: 129 96 108 194 959 66 547
Fragments arranged by size:
1414 959 817 547 283 278 195 194
172 151 146 132 129 113 108 96
86 66 30 18 18 15 12 12
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1376 2600 3747 4168 4468 5024 5060 5087
Size: 1376 1224 1147 421 300 556 36 27
Cuts at: 5087 5136 5401 5891 6012
Size: 49 265 490 121
Fragments arranged by size:
1376 1224 1147 556 490 421 300 265
121 49 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2898 4303 6012
Size: 2898 1405 1709
Fragments arranged by size:
2898 1709 1405
BsiHKAI G_wGCw'C
Cuts at: 0 4675 6012
Size: 4675 1337
BsmI GAATG_Cn'
Cuts at: 0 2210 5513 5763 6012
Size: 2210 3303 250 249
Fragments arranged by size:
3303 2210 250 249
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2662 6012
Size: 2662 3350
Bsp1286I G_dGCh'C
Cuts at: 0 1226 2837 3572 4675 5561 5978 6012
Size: 1226 1611 735 1103 886 417 34
Fragments arranged by size:
1611 1226 1103 886 735 417 34
BspHI T'CATG_A
Cuts at: 0 1427 2454 2556 6012
Size: 1427 1027 102 3456
Fragments arranged by size:
3456 1427 1027 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1967 4620 6012
Size: 1967 2653 1392
Fragments arranged by size:
2653 1967 1392
BsrBI CCG'CTC
Cuts at: 0 1155 5573 6012
Size: 1155 4418 439
Fragments arranged by size:
4418 1155 439
BsrDI GCAATG_nn'
Cuts at: 0 105 1194 6012
Size: 105 1089 4818
Fragments arranged by size:
4818 1089 105
BsrFI r'CCGG_y
Cuts at: 0 600 5221 6012
Size: 600 4621 791
Fragments arranged by size:
4621 791 600
BsrGI T'GTAC_A
Cuts at: 0 4272 5930 6012
Size: 4272 1658 82
Fragments arranged by size:
4272 1658 82
BssSI C'ACGA_G
Cuts at: 0 4008 6012
Size: 4008 2004
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3922 4844 5532 5901 6012
Size: 3922 922 688 369 111
Fragments arranged by size:
3922 922 688 369 111
BstBI TT'CG_AA
Cuts at: 0 1068 6012
Size: 1068 4944
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1885 2352 3485 6012
Size: 1885 467 1133 2527
Fragments arranged by size:
2527 1885 1133 467
BstYI r'GATC_y
Cuts at: 0 216 2307 3608 4260 4549 6012
Size: 216 2091 1301 652 289 1463
Fragments arranged by size:
2091 1463 1301 652 289 216
Bsu36I CC'TnA_GG
Cuts at: 0 2684 2864 2941 3803 6012
Size: 2684 180 77 862 2209
Fragments arranged by size:
2684 2209 862 180 77
BtgI C'CryG_G
Cuts at: 0 117 261 468 4148 4406 6012
Size: 117 144 207 3680 258 1606
Fragments arranged by size:
3680 1606 258 207 144 117
BtsI GCAGTG_nn'
Cuts at: 0 417 778 1474 1908 3099 3744 4286 4314
Size: 417 361 696 434 1191 645 542 28
Cuts at: 4314 5587 6012
Size: 1273 425
Fragments arranged by size:
1273 1191 696 645 542 434 425 417
361 28
ClaI AT'CG_AT
Cuts at: 0 1988 3200 6012
Size: 1988 1212 2812
Fragments arranged by size:
2812 1988 1212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 506 541 5490 5525 6012
Size: 506 35 4949 35 487
Fragments arranged by size:
4949 506 487 35 35
DraI TTT'AAA
Cuts at: 0 182 725 6012
Size: 182 543 5287
Fragments arranged by size:
5287 543 182
DrdI GACnn_nn'nnGTC
Cuts at: 0 2935 6012
Size: 2935 3077
EaeI y'GGCC_r
Cuts at: 0 465 708 3963 4826 5625 6012
Size: 465 243 3255 863 799 387
Fragments arranged by size:
3255 863 799 465 387 243
EarI CTCTTCn'nnn_
Cuts at: 0 2366 2393 2660 3203 3212 3300 3326 3695
Size: 2366 27 267 543 9 88 26 369
Cuts at: 3695 3704 3716 3815 4066 4115 4205 4232 4749
Size: 9 12 99 251 49 90 27 517
Cuts at: 4749 6012
Size: 1263
Fragments arranged by size:
2366 1263 543 517 369 267 251 99
90 88 49 27 27 26 12 9
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 872 1284 2230 6012
Size: 872 412 946 3782
Fragments arranged by size:
3782 946 872 412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 551 1041 1331 2018 2263 2315 2501
Size: 65 486 490 290 687 245 52 186
Cuts at: 2501 2660 2893 2934 3049 3146 3728 3743 3991
Size: 159 233 41 115 97 582 15 248
Cuts at: 3991 4632 4767 5235 5467 6012
Size: 641 135 468 232 545
Fragments arranged by size:
687 641 582 545 490 486 468 290
248 245 233 232 186 159 135 115
97 65 52 41 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3658 6012
Size: 3658 2354
EcoO109I rG'GnC_Cy
Cuts at: 0 1051 4392 5938 6012
Size: 1051 3341 1546 74
Fragments arranged by size:
3341 1546 1051 74
EcoRI G'AATT_C
Cuts at: 0 3402 6012
Size: 3402 2610
EcoRV GAT'ATC
Cuts at: 0 1461 6012
Size: 1461 4551
HaeII r_GCGC'y
Cuts at: 0 990 4325 6012
Size: 990 3335 1687
Fragments arranged by size:
3335 1687 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 434 466 1935 1967 3142 3174 3319 3351
Size: 434 32 1469 32 1175 32 145 32
Cuts at: 3351 5700 5732 6012
Size: 2349 32 280
Fragments arranged by size:
2349 1469 1175 434 280 145 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 931 2156 6012
Size: 931 1225 3856
Fragments arranged by size:
3856 1225 931
HindIII A'AGCT_T
Cuts at: 0 2275 6012
Size: 2275 3737
KasI G'GCGC_C
Cuts at: 0 4321 6012
Size: 4321 1691
MfeI C'AATT_G
Cuts at: 0 148 6012
Size: 148 5864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 2369 6012
Size: 1467 902 3643
Fragments arranged by size:
3643 1467 902
MscI TGG'CCA
Cuts at: 0 467 710 3965 4828 6012
Size: 467 243 3255 863 1184
Fragments arranged by size:
3255 1184 863 467 243
MslI CAynn'nnrTG
Cuts at: 0 895 2117 3127 4147 5287 5458 5692 5743
Size: 895 1222 1010 1020 1140 171 234 51
Cuts at: 5743 6012
Size: 269
Fragments arranged by size:
1222 1140 1020 1010 895 269 234 171
51
MspA1I CmG'CkG
Cuts at: 0 1520 2283 2600 2757 3913 4643 4757 4805
Size: 1520 763 317 157 1156 730 114 48
Cuts at: 4805 4844 5103 5274 5379 5619 6012
Size: 39 259 171 105 240 393
Fragments arranged by size:
1520 1156 763 730 393 317 259 240
171 157 114 105 48 39
NaeI GCC'GGC
Cuts at: 0 5223 6012
Size: 5223 789
NarI GG'CG_CC
Cuts at: 0 4322 6012
Size: 4322 1690
NcoI C'CATG_G
Cuts at: 0 117 261 4406 6012
Size: 117 144 4145 1606
Fragments arranged by size:
4145 1606 144 117
NgoMIV G'CCGG_C
Cuts at: 0 5221 6012
Size: 5221 791
NspI r_CATG'y
Cuts at: 0 794 834 912 3560 3651 5496 6012
Size: 794 40 78 2648 91 1845 516
Fragments arranged by size:
2648 1845 794 516 91 78 40
PciI A'CATG_T
Cuts at: 0 5492 6012
Size: 5492 520
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 5167 6012
Size: 123 5044 845
Fragments arranged by size:
5044 845 123
PfoI T'CCnGG_A
Cuts at: 0 2258 3611 6012
Size: 2258 1353 2401
Fragments arranged by size:
2401 2258 1353
PpuMI rG'GwC_Cy
Cuts at: 0 1051 4392 6012
Size: 1051 3341 1620
Fragments arranged by size:
3341 1620 1051
PshAI GACnn'nnGTC
Cuts at: 0 2332 6012
Size: 2332 3680
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5788 5820 5914 5946 6012
Size: 5788 32 94 32 66
Fragments arranged by size:
5788 94 66 32 32
PstI C_TGCA'G
Cuts at: 0 1180 1481 1661 3918 4370 4849 4891 5270
Size: 1180 301 180 2257 452 479 42 379
Cuts at: 5270 6012
Size: 742
Fragments arranged by size:
2257 1180 742 479 452 379 301 180
42
PvuII CAG'CTG
Cuts at: 0 2283 2757 3913 4757 4805 4844 5274 5379
Size: 2283 474 1156 844 48 39 430 105
Cuts at: 5379 5619 6012
Size: 240 393
Fragments arranged by size:
2283 1156 844 474 430 393 240 105
48 39
SalI G'TCGA_C
Cuts at: 0 929 6012
Size: 929 5083
SapI GCTCTTCn'nnn_
Cuts at: 0 2393 4749 6012
Size: 2393 2356 1263
Fragments arranged by size:
2393 2356 1263
SexAI A'CCwGG_T
Cuts at: 0 1561 3498 6012
Size: 1561 1937 2514
Fragments arranged by size:
2514 1937 1561
SfcI C'TryA_G
Cuts at: 0 624 1176 1477 1657 2297 2916 3914 4366
Size: 624 552 301 180 640 619 998 452
Cuts at: 4366 4845 4887 5266 6012
Size: 479 42 379 746
Fragments arranged by size:
998 746 640 624 619 552 479 452
379 301 180 42
SfoI GGC'GCC
Cuts at: 0 4323 6012
Size: 4323 1689
SmlI C'TyrA_G
Cuts at: 0 1583 1593 2171 2851 3412 3551 4295 4513
Size: 1583 10 578 680 561 139 744 218
Cuts at: 4513 5411 6012
Size: 898 601
Fragments arranged by size:
1583 898 744 680 601 578 561 218
139 10
SphI G_CATG'C
Cuts at: 0 3560 6012
Size: 3560 2452
SspI AAT'ATT
Cuts at: 0 1725 2096 6012
Size: 1725 371 3916
Fragments arranged by size:
3916 1725 371
StuI AGG'CCT
Cuts at: 0 760 4926 5884 6012
Size: 760 4166 958 128
Fragments arranged by size:
4166 958 760 128
StyI C'CwwG_G
Cuts at: 0 117 261 934 3794 4406 6012
Size: 117 144 673 2860 612 1606
Fragments arranged by size:
2860 1606 673 612 144 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 869 2740 2981 3802 4393 4476 4543 4810
Size: 869 1871 241 821 591 83 67 267
Cuts at: 4810 6012
Size: 1202
Fragments arranged by size:
1871 1202 869 821 591 267 241 83
67
TatI w'GTAC_w
Cuts at: 0 1848 1922 3276 4272 5930 6012
Size: 1848 74 1354 996 1658 82
Fragments arranged by size:
1848 1658 1354 996 82 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2432 2464 3958 3990 5124 5156 6012
Size: 2432 32 1494 32 1134 32 856
Fragments arranged by size:
2432 1494 1134 856 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3500 6012
Size: 3500 2512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 908 2632 4593 5286 5367 6012
Size: 908 1724 1961 693 81 645
Fragments arranged by size:
1961 1724 908 693 645 81
XmnI GAAnn'nnTTC
Cuts at: 0 1451 4241 6012
Size: 1451 2790 1771
Fragments arranged by size:
2790 1771 1451
ZraI GAC'GTC
Cuts at: 0 1009 2672 6012
Size: 1009 1663 3340
Fragments arranged by size:
3340 1663 1009
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AleI AlfI
AloI AlwNI ApoI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI
PsrI PstI PvuII SalI SapI SexAI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI ApaI ApaLI AscI
AseI AsiSI BcgI BfrBI BglII BmtI BsaAI BsaWI
BsiEI BsiWI BspEI BssHII BstEII BstZ17I BtgZI DraIII
EagI EcoICRI FalI FseI FspI FspAI HpaI KpnI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI XbaI XhoI XmaI