(Circular) (Six-base) MAPSORT of: pFN21AB9556.seq Check: 2541 from: 1 to: 7318 pFN21AB9556 7318 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4426 4426 Size: 7318 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4449 5128 278 Size: 53 83 186 936 2913 679 2468 Fragments arranged by size: 2913 2468 936 679 186 83 53 AccI GT'mk_AC Cuts at: 1937 3615 4047 4661 1937 Size: 1678 432 614 4594 Fragments arranged by size: 4594 1678 614 432 Acc65I G'GTAC_C Cuts at: 4639 4639 Size: 7318 AclI AA'CG_TT Cuts at: 1563 5445 5818 1563 Size: 3882 373 3063 Fragments arranged by size: 3882 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5381 6765 7033 1632 Size: 3749 1384 268 1917 Fragments arranged by size: 3749 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 4126 820 Size: 197 3109 4012 Fragments arranged by size: 4012 3109 197 AflIII A'CryG_T Cuts at: 1203 4465 4550 6217 7194 1203 Size: 3262 85 1667 977 1327 Fragments arranged by size: 3262 1667 1327 977 85 AgeI A'CCGG_T Cuts at: 6149 6149 Size: 7318 AhdI GACnn_n'nnGTC Cuts at: 6047 6047 Size: 7318 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7318 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2631 2663 3005 3037 2631 Size: 32 342 32 6912 Fragments arranged by size: 6912 342 32 32 AlwNI CAG_nnn'CTG Cuts at: 2092 5061 6633 2092 Size: 2969 1572 2777 Fragments arranged by size: 2969 2777 1572 ApaI G_GGCC'C Cuts at: 2622 2622 Size: 7318 ApaLI G'TGCA_C Cuts at: 5375 6531 5375 Size: 1156 6162 ApoI r'AATT_y Cuts at: 1493 3403 3926 4627 4853 5005 7114 1493 Size: 1910 523 701 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 701 523 226 152 AseI AT'TA_AT Cuts at: 160 1032 5872 160 Size: 872 4840 1606 Fragments arranged by size: 4840 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 AvaI C'yCGr_G Cuts at: 1870 1943 2477 2687 4643 1870 Size: 73 534 210 1956 4545 Fragments arranged by size: 4545 1956 534 210 73 AvrII C'CTAG_G Cuts at: 2857 2857 Size: 7318 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3380 3413 3380 Size: 33 7285 BamHI G'GATC_C Cuts at: 4648 5015 4648 Size: 367 6951 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 2258 2362 2512 3444 Size: 325 217 627 419 52 104 150 932 Cuts at: 3444 3872 4639 6094 618 Size: 428 767 1455 1842 Fragments arranged by size: 1842 1455 932 767 627 428 419 325 217 150 104 52 BanII G_rGCy'C Cuts at: 1391 2132 2246 2610 2622 2685 2817 3198 4223 Size: 741 114 364 12 63 132 381 1025 Cuts at: 4223 4318 4637 1391 Size: 95 319 4072 Fragments arranged by size: 4072 1025 741 381 364 319 132 114 95 63 12 BbeI G_GCGC'C Cuts at: 2262 3876 2262 Size: 1614 5704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3057 3702 4011 928 Size: 971 1158 645 309 4235 Fragments arranged by size: 4235 1158 971 645 309 BbvCI CC'TCA_GC Cuts at: 2076 2076 Size: 7318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5509 5543 1903 Size: 34 3572 34 3678 Fragments arranged by size: 3678 3572 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3766 5210 6420 3766 Size: 1444 1210 4664 Fragments arranged by size: 4664 1444 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5929 136 Size: 107 122 71 631 605 4257 1525 Fragments arranged by size: 4257 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3381 7313 3381 Size: 3932 3386 BlpI GC'TnA_GC Cuts at: 4410 4735 4410 Size: 325 6993 Bme1580I G_kGCm'C Cuts at: 1790 2211 2622 5379 6535 1790 Size: 421 411 2757 1156 2573 Fragments arranged by size: 2757 2573 1156 421 411 BmgBI CAC'GTC Cuts at: 1318 2908 2949 1318 Size: 1590 41 5687 Fragments arranged by size: 5687 1590 41 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2110 2502 5049 Size: 416 53 83 399 24 693 392 2547 Cuts at: 5049 6007 442 Size: 958 1753 Fragments arranged by size: 2547 1753 958 693 416 399 392 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7318 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2195 2195 Size: 7318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4164 4625 5978 1239 Size: 2925 461 1353 2579 Fragments arranged by size: 2925 2579 1353 461 Bpu10I CC'TnA_GC Cuts at: 2076 2076 Size: 7318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2816 4006 5443 6308 6606 2816 Size: 1190 1437 865 298 3528 Fragments arranged by size: 3528 1437 1190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2928 4601 5981 882 Size: 617 1429 1673 1380 2219 Fragments arranged by size: 2219 1673 1429 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 5109 493 Size: 711 3905 2702 Fragments arranged by size: 3905 2702 711 BsaBI GATnn'nnATC Cuts at: 7312 7312 Size: 7318 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 3873 4446 5125 Size: 53 83 186 936 726 1614 573 679 Cuts at: 5125 5507 6142 275 Size: 382 635 1451 Fragments arranged by size: 1614 1451 936 726 679 635 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5751 6149 6423 6570 6981 5751 Size: 398 274 147 411 6088 Fragments arranged by size: 6088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 2631 2637 2661 Size: 30 165 30 931 30 267 6 24 Cuts at: 2661 2667 1178 Size: 6 5829 Fragments arranged by size: 5829 931 267 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2310 2495 2675 2819 2900 3946 1186 Size: 1124 185 180 144 81 1046 4558 Fragments arranged by size: 4558 1124 1046 185 180 144 81 BseYI C'CCAG_C Cuts at: 2262 2386 3024 3918 6521 7017 2262 Size: 124 638 894 2603 496 2563 Fragments arranged by size: 2603 2563 894 638 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2455 3586 1163 Size: 573 719 1131 4895 Fragments arranged by size: 4895 1131 719 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 2581 4768 5529 6557 1540 Size: 275 186 68 512 2187 761 1028 2301 Fragments arranged by size: 2301 2187 1028 761 512 275 186 68 BsiHKAI G_wGCw'C Cuts at: 2685 4074 4223 5379 5464 6535 2685 Size: 1389 149 1156 85 1071 3468 Fragments arranged by size: 3468 1389 1156 1071 149 85 BsmI GAATG_Cn' Cuts at: 4829 4922 4829 Size: 93 7225 BsmBI CGTCTCn'nnnn_ Cuts at: 4292 4292 Size: 7318 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2211 2246 2610 2622 2685 2817 Size: 399 342 79 35 364 12 63 132 Cuts at: 2817 3198 4074 4223 4318 4637 5379 5464 6535 Size: 381 876 149 95 319 742 85 1071 Cuts at: 6535 1391 Size: 2174 Fragments arranged by size: 2174 1071 876 742 399 381 364 342 319 149 132 95 85 79 63 35 12 BspEI T'CCGG_A Cuts at: 6981 6981 Size: 7318 BspHI T'CATG_A Cuts at: 5207 6937 5207 Size: 1730 5588 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4426 4673 844 Size: 3582 247 3489 Fragments arranged by size: 3582 3489 247 BsrBI CCG'CTC Cuts at: 1394 1823 2273 5205 1394 Size: 429 450 2932 3507 Fragments arranged by size: 3507 2932 450 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3871 5813 5987 65 Size: 1183 2623 1942 174 1396 Fragments arranged by size: 2623 1942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2428 5962 6149 1700 Size: 110 618 3534 187 2869 Fragments arranged by size: 3534 2869 618 187 110 BsrGI T'GTAC_A Cuts at: 96 3903 96 Size: 3807 3511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7318 BssSI C'ACGA_G Cuts at: 5378 6390 5378 Size: 1012 6306 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7318 BstBI TT'CG_AA Cuts at: 6122 6122 Size: 7318 BstEII G'GTnAC_C Cuts at: 1184 2401 3748 1184 Size: 1217 1347 4754 Fragments arranged by size: 4754 1347 1217 BstYI r'GATC_y Cuts at: 1972 3248 3381 4648 5015 5400 5417 6869 6955 Size: 1276 133 1267 367 385 17 1452 86 Cuts at: 6955 6967 7313 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1276 1267 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3795 3795 Size: 7318 BtgI C'CryG_G Cuts at: 513 1065 2004 2444 2700 513 Size: 552 939 440 256 5131 Fragments arranged by size: 5131 939 552 440 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4488 487 Size: 683 56 3262 3317 Fragments arranged by size: 3317 3262 683 56 BtsI GCAGTG_nn' Cuts at: 2523 2970 4834 5628 5648 2523 Size: 447 1864 794 20 4193 Fragments arranged by size: 4193 1864 794 447 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7318 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7283 DraI TTT'AAA Cuts at: 3400 3931 4622 3400 Size: 531 691 6096 Fragments arranged by size: 6096 691 531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2871 6325 809 Size: 507 1555 3454 1802 Fragments arranged by size: 3454 1802 1555 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2578 4765 Size: 54 1425 50 275 18 447 301 2187 Cuts at: 4765 5654 7283 8 Size: 889 1629 43 Fragments arranged by size: 2187 1629 1425 889 447 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2578 4765 1537 Size: 275 766 2187 4090 Fragments arranged by size: 4090 2187 766 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2756 3180 3964 4085 4158 5248 1353 Size: 1403 424 784 121 73 1090 3423 Fragments arranged by size: 3423 1403 1090 784 424 121 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2074 5905 6279 6425 387 Size: 1064 138 485 3831 374 146 1280 Fragments arranged by size: 3831 1280 1064 485 374 146 138 EcoICRI GAG'CTC Cuts at: 2683 4221 2683 Size: 1538 5780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4164 4625 5381 5978 6765 7033 1239 Size: 393 2532 461 756 597 787 268 1524 Fragments arranged by size: 2532 1524 787 756 597 461 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2562 2562 Size: 7318 EcoO109I rG'GnC_Cy Cuts at: 2112 2485 2593 2619 2854 2864 3163 2112 Size: 373 108 26 235 10 299 6267 Fragments arranged by size: 6267 373 299 235 108 26 10 EcoRI G'AATT_C Cuts at: 4627 5005 4627 Size: 378 6940 FspI TGC'GCA Cuts at: 2840 5824 2840 Size: 2984 4334 HaeII r_GCGC'y Cuts at: 1678 2262 3876 6465 1678 Size: 584 1614 2589 2531 Fragments arranged by size: 2589 2531 1614 584 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3173 3205 3433 3465 4355 Size: 32 135 32 1601 32 228 32 890 Cuts at: 4355 4387 4412 4444 4537 4569 5959 5991 6033 Size: 32 25 32 93 32 1390 32 42 Cuts at: 6033 6065 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 1390 890 228 135 93 42 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2499 4662 4908 669 Size: 988 281 561 2163 246 3079 Fragments arranged by size: 3079 2163 988 561 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7318 HpaI GTT'AAC Cuts at: 4908 4908 Size: 7318 KasI G'GCGC_C Cuts at: 2258 3872 2258 Size: 1614 5704 KpnI G_GTAC'C Cuts at: 4643 4643 Size: 7318 MfeI C'AATT_G Cuts at: 4563 4917 4563 Size: 354 6964 MluI A'CGCG_T Cuts at: 7194 7194 Size: 7318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 3486 6432 6616 1305 Size: 1515 666 2946 184 2007 Fragments arranged by size: 2946 2007 1515 666 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 10 Size: 54 1425 343 447 5049 Fragments arranged by size: 5049 1425 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4266 5276 5635 5794 518 Size: 648 3100 1010 359 159 2042 Fragments arranged by size: 3100 2042 1010 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 2446 3922 4320 4347 4734 5411 6559 Size: 660 180 1476 398 27 387 677 1148 Cuts at: 6559 6804 1606 Size: 245 2120 Fragments arranged by size: 2120 1476 1148 677 660 398 387 245 180 27 NarI GG'CG_CC Cuts at: 2259 3873 2259 Size: 1614 5704 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5827 Fragments arranged by size: 5827 939 552 NdeI CA'TA_TG Cuts at: 387 3589 387 Size: 3202 4116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7318 NotI GC'GGCC_GC Cuts at: 4765 4765 Size: 7318 NspI r_CATG'y Cuts at: 1132 1229 4554 4676 6221 1132 Size: 97 3325 122 1545 2229 Fragments arranged by size: 3325 2229 1545 122 97 PasI CC'CwG_GG Cuts at: 1348 2869 2918 4333 1348 Size: 1521 49 1415 4333 Fragments arranged by size: 4333 1521 1415 49 PciI A'CATG_T Cuts at: 4550 6217 4550 Size: 1667 5651 PflMI CCAn_nnn'nTGG Cuts at: 1108 2092 2393 1108 Size: 984 301 6033 Fragments arranged by size: 6033 984 301 PmeI GTTT'AAAC Cuts at: 4622 4622 Size: 7318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2592 2624 2631 2663 5387 5419 6900 6932 2592 Size: 32 7 32 2724 32 1481 32 2978 Fragments arranged by size: 2978 2724 1481 32 32 32 32 7 PpuMI rG'GwC_Cy Cuts at: 2485 2593 2864 2485 Size: 108 271 6939 Fragments arranged by size: 6939 271 108 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7318 PsiI TTA'TAA Cuts at: 4888 4888 Size: 7318 PspOMI G'GGCC_C Cuts at: 2618 2618 Size: 7318 PstI C_TGCA'G Cuts at: 830 4363 4437 4670 830 Size: 3533 74 233 3478 Fragments arranged by size: 3533 3478 233 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7318 PvuII CAG'CTG Cuts at: 3922 3922 Size: 7318 SacI G_AGCT'C Cuts at: 2685 4223 2685 Size: 1538 5780 SacII CC_GC'GG Cuts at: 2447 2447 Size: 7318 SalI G'TCGA_C Cuts at: 1936 4660 1936 Size: 2724 4594 SapI GCTCTTCn'nnn_ Cuts at: 4158 4158 Size: 7318 SbfI CC_TGCA'GG Cuts at: 4437 4670 4437 Size: 233 7085 ScaI AGT'ACT Cuts at: 5566 7278 5566 Size: 1712 5606 SfcI C'TryA_G Cuts at: 826 1045 3700 4359 4433 4666 5801 6482 6673 Size: 219 2655 659 74 233 1135 681 191 Cuts at: 6673 826 Size: 1471 Fragments arranged by size: 2655 1471 1135 681 659 233 219 191 74 SfoI GGC'GCC Cuts at: 2260 3874 2260 Size: 1614 5704 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7318 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7318 SmaI CCC'GGG Cuts at: 1872 4645 1872 Size: 2773 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 2831 4021 4126 5422 6323 Size: 197 926 744 144 1190 105 1296 901 Cuts at: 6323 6585 820 Size: 262 1553 Fragments arranged by size: 1553 1296 1190 926 901 744 262 197 144 105 SnaBI TAC'GTA Cuts at: 493 5109 493 Size: 4616 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7318 SphI G_CATG'C Cuts at: 1132 4676 1132 Size: 3544 3774 SspI AAT'ATT Cuts at: 5 52 3463 5242 5 Size: 47 3411 1779 2081 Fragments arranged by size: 3411 2081 1779 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7318 StyI C'CwwG_G Cuts at: 513 1065 2004 2857 4539 4757 513 Size: 552 939 853 1682 218 3074 Fragments arranged by size: 3074 1682 939 853 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 4380 5345 5362 5515 5700 6145 Size: 1255 779 2021 965 17 153 185 445 Cuts at: 6145 325 Size: 1498 Fragments arranged by size: 2021 1498 1255 965 779 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2711 3903 Size: 274 80 33 51 1443 327 407 1192 Cuts at: 3903 5564 7276 96 Size: 1661 1712 138 Fragments arranged by size: 1712 1661 1443 1192 407 327 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2334 2366 3557 3589 7278 7310 2334 Size: 32 1191 32 3689 32 2342 Fragments arranged by size: 3689 2342 1191 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4448 1316 Size: 3132 4186 XbaI T'CTAG_A Cuts at: 4654 4654 Size: 7318 XhoI C'TCGA_G Cuts at: 1943 2687 1943 Size: 744 6574 XmaI C'CCGG_G Cuts at: 1870 4643 1870 Size: 2773 4545 XmnI GAAnn'nnTTC Cuts at: 5447 5447 Size: 7318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4447 5126 276 Size: 53 83 186 936 2913 679 2468 Fragments arranged by size: 2913 2468 936 679 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI