Restriction map for
pFN21AB9555
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbsI BbvI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tru9I TseI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AflIII 1256 ACGTGT A^C[GA][CT]GT
BamHI 899 GGATCC G^GATCC
BbvCI 300 CCTCAGC CCTCAGC(-5/-2)
BcgI 1296 GCACCTGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 233 GTATCC GTATCC(6/5)
BlpI 623 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 623 GCTCAGC GC^T[ACGT]AGC
BsaAI 1255 TACGTG [CT]AC^GT[GA]
BseRI 1044 CTCCTC GAGGAG(10/8)
BsmI 682 GAATGC GAATGC(1/-1)
BstXI 1014 CCAGAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 623 GCTCAGC GC^T[ACGT]AGC
DraIII 472 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 731 GAATTC G^AATTC
Fnu4HI 1271 GCGGC GC^[ACGT]GC
ItaI 1271 GCGGC GC^[ACGT]GC
MaeII 1256 ACGT A^CGT
MspA1I 895 CAGCGG C[AC]G^C[GT]G
NspI 305 GCATGT [GA]CATG^[CT]
SexAI 473 ACCTGGT A^CC[AT]GGT
SgrAI 1074 CACCGGTG C[GA]^CCGG[CT]G
SmlI 210 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 195 GAATTT [GA]^AATT[CT]
731 GAATTC
AgeI 1075 ACCGGT A^CCGGT
1205 ACCGGT
ApoI 195 GAATTT [GA]^AATT[CT]
731 GAATTC
BanI 554 GGCACC G^G[CT][GA]CC
1202 GGCACC
BpmI 286 CTCCAG CTGGAG(16/14)
708 CTCCAG
BsaI 31 GAGACC GGTCTC(1/5)
747 GAGACC
BsgI 350 GTGCAG GTGCAG(16/14)
675 GTGCAG
BsmAI 31 GAGAC GTCTC(1/5)
747 GAGAC
BsmFI 95 GGGAC GGGAC(10/14)
368 GGGAC
BssSI 724 CACGAG CACGAG(-5/-1)
838 CACGAG
BstAPI 677 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1262 GCAATCTGTGC
CfoI 1065 GCGC GCG^C
1132 GCGC
EarI 268 CTCTTC CTCTTC(1/4)
328 GAAGAG
HhaI 1065 GCGC GCG^C
1132 GCGC
HinP1I 1065 GCGC G^CGC
1132 GCGC
Ksp632I 268 CTCTTC CTCTTC(1/4)
328 GAAGAG
MslI 920 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1072 CACACCGGTG
NlaIII 306 CATG CATG^
341 CATG
PinAI 1075 ACCGGT A^CCGGT
1205 ACCGGT
PleI 370 GACTC GAGTC(4/5)
58 GAGTC
SapI 328 GAAGAGC GCTCTTC(1/4)
267 GCTCTTC
TaqI 426 TCGA T^CGA
1220 TCGA
Tsp509I 196 AATT ^AATT
732 AATT
TthHB8I 426 TCGA T^CGA
1220 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI Asp700I AspHI
AvaII BanII BcnI BmyI BsaJI
BsaWI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI FokI HaeIII HapII HinfI
HpaII HphI MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIV PpuMI SacI
Sau3AI Sau96I ScrFI SfaNI StuI
TfiI Tsp45I TspRI XhoII XmnI