(Circular) (Six-base) MAPSORT of: pFN21AB9555.seq Check: 9943 from: 1 to: 6043 pFN21AB9555 6043 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 2559 3191 2475 Size: 84 632 5327 Fragments arranged by size: 5327 632 84 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2434 2518 2938 3022 4106 5490 5758 Size: 464 338 84 420 84 1084 1384 268 Cuts at: 5758 1632 Size: 1917 Fragments arranged by size: 1917 1384 1084 464 420 338 268 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 3261 4942 5919 1203 Size: 2058 1681 977 1327 Fragments arranged by size: 2058 1681 1327 977 AgeI A'CCGG_T Cuts at: 3080 3210 4874 3080 Size: 130 1664 4249 Fragments arranged by size: 4249 1664 130 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 3081 1166 Size: 1915 4128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 3786 5358 3786 Size: 1572 4471 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 2200 2736 3352 3578 3730 5839 1493 Size: 707 536 616 226 152 2109 1697 Fragments arranged by size: 2109 1697 707 616 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 2904 3373 3740 2904 Size: 469 367 5207 Fragments arranged by size: 5207 469 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 3207 3364 4819 618 Size: 325 217 627 772 648 157 1455 1842 Fragments arranged by size: 1842 1455 772 648 627 325 217 157 BanII G_rGCy'C Cuts at: 1391 2274 2340 2462 2546 2630 2714 2798 2966 Size: 883 66 122 84 84 84 84 168 Cuts at: 2966 3050 3299 3362 1391 Size: 84 249 63 4072 Fragments arranged by size: 4072 883 249 168 122 84 84 84 84 84 66 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BbvCI CC'TCA_GC Cuts at: 2306 2306 Size: 6043 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3290 3324 4234 4268 1903 Size: 34 1353 34 910 34 3678 Fragments arranged by size: 3678 1353 910 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2249 3935 5145 2249 Size: 1686 1210 3147 Fragments arranged by size: 3147 1686 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 2629 3460 2629 Size: 831 5212 Bme1580I G_kGCm'C Cuts at: 1790 4104 5260 1790 Size: 2314 1156 2573 Fragments arranged by size: 2573 2314 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 2502 2922 Size: 416 53 83 399 24 917 168 420 Cuts at: 2922 3774 4732 442 Size: 852 958 1753 Fragments arranged by size: 1753 958 917 852 420 416 399 168 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2698 4703 1239 Size: 1037 422 2005 2579 Fragments arranged by size: 2579 2005 1037 422 Bpu10I CC'TnA_GC Cuts at: 2306 2555 2797 2804 2306 Size: 249 242 7 5545 Fragments arranged by size: 5545 249 242 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2200 4168 5033 5331 2200 Size: 1968 865 298 2912 Fragments arranged by size: 2912 1968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2746 4706 882 Size: 617 531 716 1960 2219 Fragments arranged by size: 2219 1960 716 617 531 BsaAI yAC'GTr Cuts at: 493 1204 3262 3834 493 Size: 711 2058 572 2702 Fragments arranged by size: 2702 2058 711 572 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3850 4232 4867 275 Size: 53 83 186 936 2317 382 635 1451 Fragments arranged by size: 2317 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2660 3080 3210 4476 4874 5148 5295 5706 2660 Size: 420 130 1266 398 274 147 411 2997 Fragments arranged by size: 2997 1266 420 411 398 274 147 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3040 1186 Size: 1854 4189 BseYI C'CCAG_C Cuts at: 2017 5246 5742 2017 Size: 3229 496 2318 Fragments arranged by size: 3229 2318 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2376 2701 1163 Size: 573 640 325 4505 Fragments arranged by size: 4505 640 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3493 4254 5282 1540 Size: 275 186 1492 761 1028 2301 Fragments arranged by size: 2301 1492 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2274 2462 2546 2630 2714 2798 2966 2982 Size: 58 188 84 84 84 84 168 16 Cuts at: 2982 3050 3066 3231 3299 4104 4189 5260 2216 Size: 68 16 165 68 805 85 1071 2999 Fragments arranged by size: 2999 1071 805 188 168 165 85 84 84 84 84 68 68 58 16 16 BsmI GAATG_Cn' Cuts at: 2693 3554 3647 2693 Size: 861 93 5089 Fragments arranged by size: 5089 861 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2274 2340 2462 2546 2630 2714 Size: 399 426 58 66 122 84 84 84 Cuts at: 2714 2798 2966 2982 3050 3066 3231 3299 3362 Size: 84 168 16 68 16 165 68 63 Cuts at: 3362 4104 4189 5260 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 168 165 122 85 84 84 84 84 68 68 66 63 58 16 16 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 2559 3191 3398 844 Size: 1631 84 632 207 3489 Fragments arranged by size: 3489 1631 632 207 84 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3080 3164 3210 4687 4874 1700 Size: 110 1270 84 46 1477 187 2869 Fragments arranged by size: 2869 1477 1270 187 110 84 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 2729 2843 4103 5115 2729 Size: 114 1260 1012 3657 Fragments arranged by size: 3657 1260 1012 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 2688 3273 794 Size: 1894 585 3564 Fragments arranged by size: 3564 1894 585 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstXI CCAn_nnnn'nTGG Cuts at: 3026 3026 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2154 2652 2820 2904 3373 3740 4125 4142 Size: 182 498 168 84 469 367 385 17 Cuts at: 4142 5594 5680 5692 6038 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 498 469 385 367 346 182 168 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2949 487 Size: 683 56 1723 3581 Fragments arranged by size: 3581 1723 683 56 BtsI GCAGTG_nn' Cuts at: 3559 4353 4373 3559 Size: 794 20 5229 Fragments arranged by size: 5229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DraIII CAC_nnn'GTG Cuts at: 2482 2482 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008 Size: 54 1425 50 275 18 1660 889 1629 Cuts at: 6008 8 Size: 43 Fragments arranged by size: 1660 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 2328 3973 1353 Size: 926 49 1645 3423 Fragments arranged by size: 3423 1645 926 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2609 4630 5004 5150 387 Size: 1064 138 1020 2021 374 146 1280 Fragments arranged by size: 2021 1280 1064 1020 374 146 138 EcoICRI GAG'CTC Cuts at: 2272 2460 2544 2628 2712 2796 2964 3048 3297 Size: 188 84 84 84 84 168 84 249 Cuts at: 3297 2272 Size: 5018 Fragments arranged by size: 5018 249 188 168 84 84 84 84 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2276 2434 2518 2698 2938 3022 Size: 393 464 180 158 84 180 240 84 Cuts at: 3022 4106 4703 5490 5758 1239 Size: 1084 597 787 268 1524 Fragments arranged by size: 1524 1084 787 597 464 393 268 240 180 180 158 84 84 EcoO109I rG'GnC_Cy Cuts at: 2092 2101 2381 2670 2092 Size: 9 280 289 5465 Fragments arranged by size: 5465 289 280 9 EcoRI G'AATT_C Cuts at: 2736 3352 3730 2736 Size: 616 378 5049 Fragments arranged by size: 5049 616 378 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 5190 1678 Size: 3512 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2743 2775 2797 2829 3298 Size: 32 135 32 1171 32 22 32 469 Cuts at: 3298 3330 4684 4716 4758 4790 1373 Size: 32 1354 32 42 32 2626 Fragments arranged by size: 2626 1354 1171 469 135 42 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 2497 2917 2991 5157 5341 1305 Size: 1020 172 420 74 2166 184 2007 Fragments arranged by size: 2166 2007 1020 420 184 172 74 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4221 Fragments arranged by size: 4221 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2929 3081 4001 4360 4519 518 Size: 648 1763 152 920 359 159 2042 Fragments arranged by size: 2042 1763 920 648 359 159 152 MspA1I CmG'CkG Cuts at: 1606 2902 3459 4136 5284 5529 1606 Size: 1296 557 677 1148 245 2120 Fragments arranged by size: 2120 1296 1148 677 557 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4552 Fragments arranged by size: 4552 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 2314 3401 4946 1132 Size: 97 1085 1087 1545 2229 Fragments arranged by size: 2229 1545 1087 1085 97 PasI CC'CwG_GG Cuts at: 1348 2084 1348 Size: 736 5307 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2101 2381 2092 Size: 9 280 5754 Fragments arranged by size: 5754 280 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 3395 830 Size: 2565 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 SacI G_AGCT'C Cuts at: 2274 2462 2546 2630 2714 2798 2966 3050 3299 Size: 188 84 84 84 84 168 84 249 Cuts at: 3299 2274 Size: 5018 Fragments arranged by size: 5018 249 188 168 84 84 84 84 84 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SanDI GG'GwC_CC Cuts at: 2101 2101 Size: 6043 SapI GCTCTTCn'nnn_ Cuts at: 2279 2328 2279 Size: 49 5994 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SexAI A'CCwGG_T Cuts at: 2478 2478 Size: 6043 SfcI C'TryA_G Cuts at: 826 1045 3391 4526 5207 5398 826 Size: 219 2346 1135 681 191 1471 Fragments arranged by size: 2346 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 3080 1700 Size: 1380 4663 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2215 4147 5048 5310 820 Size: 197 926 272 1932 901 262 1553 Fragments arranged by size: 1932 1553 926 901 272 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2447 2531 2615 2802 2951 3119 1517 Size: 930 84 84 187 149 168 4441 Fragments arranged by size: 4441 930 187 168 149 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3482 513 Size: 552 939 1478 3074 Fragments arranged by size: 3074 1478 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4070 4087 4240 4425 4870 325 Size: 1255 2490 17 153 185 445 1498 Fragments arranged by size: 2490 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4289 6001 96 Size: 274 80 33 51 1443 2312 1712 138 Fragments arranged by size: 2312 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2874 2906 6003 6035 2874 Size: 32 3097 32 2882 Fragments arranged by size: 3097 2882 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 2447 2531 2615 2699 2783 2951 3035 4172 2447 Size: 84 84 84 84 168 84 1137 4318 Fragments arranged by size: 4318 1137 168 84 84 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI XcmI