Restriction map for
pFN21AB9555
(Linear) (Six-base) MAPSORT of: KIBB9555.seq Check: 8123 from: 1 to: 1338
KIBB9555.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 554 1186 1338
Size: 470 84 632 152
Fragments arranged by size:
632 470 152 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 429 513 933 1017 1338
Size: 91 338 84 420 84 321
Fragments arranged by size:
420 338 321 91 84 84
AflIII A'CryG_T
Cuts at: 0 1256 1338
Size: 1256 82
AgeI A'CCGG_T
Cuts at: 0 1075 1205 1338
Size: 1075 130 133
Fragments arranged by size:
1075 133 130
AleI CACnn'nnGTG
Cuts at: 0 1076 1338
Size: 1076 262
ApoI r'AATT_y
Cuts at: 0 195 731 1338
Size: 195 536 607
Fragments arranged by size:
607 536 195
BamHI G'GATC_C
Cuts at: 0 899 1338
Size: 899 439
BanI G'GyrC_C
Cuts at: 0 554 1202 1338
Size: 554 648 136
Fragments arranged by size:
648 554 136
BanII G_rGCy'C
Cuts at: 0 269 335 457 541 625 709 793 961
Size: 269 66 122 84 84 84 84 168
Cuts at: 961 1045 1294 1338
Size: 84 249 44
Fragments arranged by size:
269 249 168 122 84 84 84 84
84 66 44
BbvCI CC'TCA_GC
Cuts at: 0 301 1338
Size: 301 1037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1285 1319 1338
Size: 1285 34 19
Fragments arranged by size:
1285 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 244 1338
Size: 244 1094
BlpI GC'TnA_GC
Cuts at: 0 624 1338
Size: 624 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 497 917 1338
Size: 329 168 420 421
Fragments arranged by size:
421 420 329 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 693 1338
Size: 271 422 645
Fragments arranged by size:
645 422 271
Bpu10I CC'TnA_GC
Cuts at: 0 301 550 792 799 1338
Size: 301 249 242 7 539
Fragments arranged by size:
539 301 249 242 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1338
Size: 195 1143
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 741 1338
Size: 25 716 597
Fragments arranged by size:
716 597 25
BsaAI yAC'GTr
Cuts at: 0 1257 1338
Size: 1257 81
BsaWI w'CCGG_w
Cuts at: 0 655 1075 1205 1338
Size: 655 420 130 133
Fragments arranged by size:
655 420 133 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1035 1338
Size: 1035 303
BseYI C'CCAG_C
Cuts at: 0 12 1338
Size: 12 1326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 696 1338
Size: 371 325 642
Fragments arranged by size:
642 371 325
BsiHKAI G_wGCw'C
Cuts at: 0 211 269 457 541 625 709 793 961
Size: 211 58 188 84 84 84 84 168
Cuts at: 961 977 1045 1061 1226 1294 1338
Size: 16 68 16 165 68 44
Fragments arranged by size:
211 188 168 165 84 84 84 84
68 68 58 44 16 16
BsmI GAATG_Cn'
Cuts at: 0 688 1338
Size: 688 650
Bsp1286I G_dGCh'C
Cuts at: 0 211 269 335 457 541 625 709 793
Size: 211 58 66 122 84 84 84 84
Cuts at: 793 961 977 1045 1061 1226 1294 1338
Size: 168 16 68 16 165 68 44
Fragments arranged by size:
211 168 165 122 84 84 84 84
68 68 66 58 44 16 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 554 1186 1338
Size: 470 84 632 152
Fragments arranged by size:
632 470 152 84
BsrFI r'CCGG_y
Cuts at: 0 1075 1159 1205 1338
Size: 1075 84 46 133
Fragments arranged by size:
1075 133 84 46
BssSI C'ACGA_G
Cuts at: 0 724 838 1338
Size: 724 114 500
Fragments arranged by size:
724 500 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 683 1268 1338
Size: 683 585 70
Fragments arranged by size:
683 585 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1021 1338
Size: 1021 317
BstYI r'GATC_y
Cuts at: 0 149 647 815 899 1338
Size: 149 498 168 84 439
Fragments arranged by size:
498 439 168 149 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 944 1338
Size: 944 394
DraIII CAC_nnn'GTG
Cuts at: 0 477 1338
Size: 477 861
EarI CTCTTCn'nnn_
Cuts at: 0 274 323 1338
Size: 274 49 1015
Fragments arranged by size:
1015 274 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 1338
Size: 604 734
EcoICRI GAG'CTC
Cuts at: 0 267 455 539 623 707 791 959 1043
Size: 267 188 84 84 84 84 168 84
Cuts at: 1043 1292 1338
Size: 249 46
Fragments arranged by size:
267 249 188 168 84 84 84 84
84 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 271 429 513 693 933 1017 1338
Size: 91 180 158 84 180 240 84 321
Fragments arranged by size:
321 240 180 180 158 91 84 84
EcoO109I rG'GnC_Cy
Cuts at: 0 87 96 376 665 1338
Size: 87 9 280 289 673
Fragments arranged by size:
673 289 280 87 9
EcoRI G'AATT_C
Cuts at: 0 731 1338
Size: 731 607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 738 770 792 824 1293 1325 1338
Size: 738 32 22 32 469 32 13
Fragments arranged by size:
738 469 32 32 32 22 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 492 912 986 1338
Size: 320 172 420 74 352
Fragments arranged by size:
420 352 320 172 74
MslI CAynn'nnrTG
Cuts at: 0 924 1076 1338
Size: 924 152 262
Fragments arranged by size:
924 262 152
MspA1I CmG'CkG
Cuts at: 0 897 1338
Size: 897 441
NspI r_CATG'y
Cuts at: 0 309 1338
Size: 309 1029
PasI CC'CwG_GG
Cuts at: 0 79 1338
Size: 79 1259
PpuMI rG'GwC_Cy
Cuts at: 0 87 96 376 1338
Size: 87 9 280 962
Fragments arranged by size:
962 280 87 9
SacI G_AGCT'C
Cuts at: 0 269 457 541 625 709 793 961 1045
Size: 269 188 84 84 84 84 168 84
Cuts at: 1045 1294 1338
Size: 249 44
Fragments arranged by size:
269 249 188 168 84 84 84 84
84 44
SanDI GG'GwC_CC
Cuts at: 0 96 1338
Size: 96 1242
SapI GCTCTTCn'nnn_
Cuts at: 0 274 323 1338
Size: 274 49 1015
Fragments arranged by size:
1015 274 49
SexAI A'CCwGG_T
Cuts at: 0 473 1338
Size: 473 865
SgrAI Cr'CCGG_yG
Cuts at: 0 1075 1338
Size: 1075 263
SmlI C'TyrA_G
Cuts at: 0 210 1338
Size: 210 1128
StuI AGG'CCT
Cuts at: 0 442 526 610 797 946 1114 1338
Size: 442 84 84 187 149 168 224
Fragments arranged by size:
442 224 187 168 149 84 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 1338
Size: 869 32 437
Fragments arranged by size:
869 437 32
XmnI GAAnn'nnTTC
Cuts at: 0 442 526 610 694 778 946 1030 1338
Size: 442 84 84 84 84 168 84 308
Fragments arranged by size:
442 308 168 84 84 84 84 84
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI ApoI BamHI BanI
BanII BbvCI BcgI BciVI BlpI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI
BtgZI DraIII EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I MmeI MslI MspA1I NspI PasI PpuMI SacI
SanDI SapI SexAI SgrAI SmlI StuI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BclI BfrBI BglI BglII
Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DrdI EaeI EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI