(Circular) (Six-base) MAPSORT of: pFN21AB9553.seq Check: 5785 from: 1 to: 5488 pFN21AB9553 5488 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2518 2518 Size: 5488 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2758 3298 278 Size: 53 83 186 936 1222 540 2468 Fragments arranged by size: 2468 1222 936 540 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 2831 1937 Size: 765 129 4594 Fragments arranged by size: 4594 765 129 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 2787 3615 3988 1563 Size: 1224 828 373 3063 Fragments arranged by size: 3063 1224 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 2576 3551 4935 5203 1632 Size: 825 119 975 1384 268 1917 Fragments arranged by size: 1917 1384 975 825 268 119 AfeI AGC'GCT Cuts at: 2411 2411 Size: 5488 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 4387 5364 1203 Size: 3184 977 1327 Fragments arranged by size: 3184 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 2110 2605 2674 3231 4803 2110 Size: 495 69 557 1572 2795 Fragments arranged by size: 2795 1572 557 495 69 ApaLI G'TGCA_C Cuts at: 2514 3545 4701 2514 Size: 1031 1156 3301 Fragments arranged by size: 3301 1156 1031 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 2780 4042 160 Size: 872 1748 1262 1606 Fragments arranged by size: 1748 1606 1262 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2210 2813 1870 Size: 73 267 603 4545 Fragments arranged by size: 4545 603 267 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2809 4264 618 Size: 325 217 627 335 687 1455 1842 Fragments arranged by size: 1842 1455 687 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2095 2210 2693 2807 1391 Size: 704 115 483 114 4072 Fragments arranged by size: 4072 704 483 115 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4517 BbvCI CC'TCA_GC Cuts at: 2225 2225 Size: 5488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 4099 136 Size: 107 122 71 631 605 433 1994 1525 Fragments arranged by size: 1994 1525 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2518 3549 4705 1790 Size: 728 1031 1156 2573 Fragments arranged by size: 2573 1156 1031 728 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 2635 2711 Size: 416 53 83 399 24 1116 102 76 Cuts at: 2711 3219 4177 442 Size: 508 958 1753 Fragments arranged by size: 1753 1116 958 508 416 399 102 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2704 4148 1239 Size: 1298 167 1444 2579 Fragments arranged by size: 2579 1444 1298 167 Bpu10I CC'TnA_GC Cuts at: 2204 2225 2204 Size: 21 5467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3613 4478 4776 3613 Size: 865 298 4325 Fragments arranged by size: 4325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2513 3279 493 Size: 711 1309 766 2702 Fragments arranged by size: 2702 1309 766 711 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2755 3295 3677 4312 Size: 53 83 186 936 1222 540 382 635 Cuts at: 4312 275 Size: 1451 Fragments arranged by size: 1451 1222 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3921 4319 4593 4740 5151 3921 Size: 398 274 147 411 4258 Fragments arranged by size: 4258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5263 Fragments arranged by size: 5263 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2242 2456 2701 1186 Size: 1042 14 214 245 3973 Fragments arranged by size: 3973 1042 245 214 14 BseYI C'CCAG_C Cuts at: 4691 5187 4691 Size: 496 4992 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 4099 Fragments arranged by size: 4099 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2100 2938 3699 4727 1540 Size: 275 186 36 63 838 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 275 186 63 36 BsiHKAI G_wGCw'C Cuts at: 2518 2693 3549 3634 4705 2518 Size: 175 856 85 1071 3301 Fragments arranged by size: 3301 1071 856 175 85 BsmI GAATG_Cn' Cuts at: 2621 2999 3092 2621 Size: 378 93 5017 Fragments arranged by size: 5017 378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2210 2518 2693 2807 3549 3634 Size: 399 305 115 308 175 114 742 85 Cuts at: 3634 4705 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 305 175 115 114 85 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2518 2843 844 Size: 1674 325 3489 Fragments arranged by size: 3489 1674 325 BsrBI CCG'CTC Cuts at: 1394 1823 2116 2160 3375 1394 Size: 429 293 44 1215 3507 Fragments arranged by size: 3507 1215 429 293 44 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2099 4132 4319 1700 Size: 110 289 2033 187 2869 Fragments arranged by size: 2869 2033 289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 2177 1925 Size: 252 5236 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 2530 794 Size: 1736 3752 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5488 BstYI r'GATC_y Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483 Size: 846 367 385 17 1452 86 12 346 Cuts at: 5483 1972 Size: 1977 Fragments arranged by size: 1977 1452 846 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2567 513 Size: 552 939 563 3434 Fragments arranged by size: 3434 939 563 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3798 3818 3004 Size: 794 20 4674 Fragments arranged by size: 4674 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2097 2101 Size: 54 1425 50 275 18 204 63 4 Cuts at: 2101 2478 2935 3824 5453 8 Size: 377 457 889 1629 43 Fragments arranged by size: 1629 1425 889 457 377 275 204 63 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2034 2097 2935 1537 Size: 275 222 63 838 4090 Fragments arranged by size: 4090 838 275 222 63 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2433 2595 3418 1353 Size: 966 114 162 823 3423 Fragments arranged by size: 3423 966 823 162 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 2462 4075 4449 4595 387 Size: 1064 138 482 391 1613 374 146 1280 Fragments arranged by size: 1613 1280 1064 482 391 374 146 138 EcoICRI GAG'CTC Cuts at: 2691 2691 Size: 5488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2457 2537 2576 2704 3551 4148 4935 Size: 393 825 80 39 128 847 597 787 Cuts at: 4935 5203 1239 Size: 268 1524 Fragments arranged by size: 1524 847 825 787 597 393 268 128 80 39 EcoO109I rG'GnC_Cy Cuts at: 2043 2107 2713 2043 Size: 64 606 4818 Fragments arranged by size: 4818 606 64 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 EcoRV GAT'ATC Cuts at: 2610 2610 Size: 5488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2075 2075 Size: 5488 FseI GG_CCGG'CC Cuts at: 2103 2103 Size: 5488 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 2194 2225 2413 4635 1678 Size: 516 31 188 2222 2531 Fragments arranged by size: 2531 2222 516 188 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2178 2210 2587 2619 4129 Size: 32 135 32 606 32 377 32 1510 Cuts at: 4129 4161 4203 4235 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1510 606 377 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3666 Fragments arranged by size: 3666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3446 3805 3964 518 Size: 648 843 1437 359 159 2042 Fragments arranged by size: 2042 1437 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2904 3581 4729 4974 1606 Size: 1298 677 1148 245 2120 Fragments arranged by size: 2120 1298 1148 677 245 NaeI GCC'GGC Cuts at: 2101 2101 Size: 5488 NcoI C'CATG_G Cuts at: 513 1065 2004 2567 513 Size: 552 939 563 3434 Fragments arranged by size: 3434 939 563 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NgoMIV G'CCGG_C Cuts at: 2099 2099 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2043 2713 2043 Size: 670 4818 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PstI C_TGCA'G Cuts at: 830 2526 2840 830 Size: 1696 314 3478 Fragments arranged by size: 3478 1696 314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SacI G_AGCT'C Cuts at: 2693 2693 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SanDI GG'GwC_CC Cuts at: 2043 2043 Size: 5488 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2522 2836 3971 4652 4843 826 Size: 219 1477 314 1135 681 191 1471 Fragments arranged by size: 1477 1471 1135 681 314 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2105 2105 Size: 5488 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3592 4493 4755 820 Size: 197 926 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5488 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 2567 2927 513 Size: 552 939 450 113 360 3074 Fragments arranged by size: 3074 939 552 450 360 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3515 3532 3685 3870 4315 325 Size: 1255 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3734 5446 96 Size: 274 80 33 51 1443 1757 1712 138 Fragments arranged by size: 1757 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5448 5480 5448 Size: 32 5456 Tth111I GACn'n_nGTC Cuts at: 1316 2641 1316 Size: 1325 4163 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2756 3296 276 Size: 53 83 186 936 1222 540 2468 Fragments arranged by size: 2468 1222 936 540 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfoI SrfI SwaI