(Circular) (Six-base) MAPSORT of: pFN21AB9551.seq Check: 2671 from: 1 to: 5860 pFN21AB9551 5860 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 2398 3181 2398 Size: 783 5077 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2909 2971 3923 5307 5575 1632 Size: 1277 62 952 1384 268 1917 Fragments arranged by size: 1917 1384 1277 952 268 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2850 4759 5736 1203 Size: 1647 1909 977 1327 Fragments arranged by size: 1909 1647 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 2264 1166 Size: 1098 4762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2602 3080 3603 5175 2602 Size: 478 523 1572 3287 Fragments arranged by size: 3287 1572 523 478 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2552 3185 1870 Size: 73 609 633 4545 Fragments arranged by size: 4545 633 609 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2398 3051 3181 4636 618 Size: 325 217 627 611 653 130 1455 1842 Fragments arranged by size: 1842 1455 653 627 611 325 217 130 BanII G_rGCy'C Cuts at: 1391 2352 3179 1391 Size: 961 827 4072 Fragments arranged by size: 4072 961 827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2981 928 Size: 971 1082 3807 Fragments arranged by size: 3807 1082 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2014 2048 4051 4085 1903 Size: 34 77 34 2003 34 3678 Fragments arranged by size: 3678 2003 77 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2237 3752 4962 2237 Size: 1515 1210 3135 Fragments arranged by size: 3135 1515 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3146 4471 136 Size: 107 122 71 631 605 1474 1325 1525 Fragments arranged by size: 1525 1474 1325 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 2021 2960 3277 2021 Size: 939 317 4604 Fragments arranged by size: 4604 939 317 Bme1580I G_kGCm'C Cuts at: 1790 3056 3921 5077 1790 Size: 1266 865 1156 2573 Fragments arranged by size: 2573 1266 1156 865 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2176 2786 3083 4520 1239 Size: 842 95 610 297 1437 2579 Fragments arranged by size: 2579 1437 842 610 297 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 3020 5854 3020 Size: 2834 3026 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2610 2645 3133 3667 Size: 53 83 186 936 1077 35 488 534 Cuts at: 3667 4049 4684 275 Size: 382 635 1451 Fragments arranged by size: 1451 1077 936 635 534 488 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 3024 4293 4691 4965 5112 5523 3024 Size: 1269 398 274 147 411 3361 Fragments arranged by size: 3361 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 2894 2924 1178 Size: 30 165 30 1356 30 105 30 4114 Fragments arranged by size: 4114 1356 165 105 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 2521 2905 5063 5559 2521 Size: 384 2158 496 2822 Fragments arranged by size: 2822 2158 496 384 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2900 3921 4006 5077 2900 Size: 1021 85 1071 3683 Fragments arranged by size: 3683 1071 1021 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 BsmBI CGTCTCn'nnnn_ Cuts at: 2601 2601 Size: 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2900 3056 3179 3921 4006 5077 Size: 399 562 548 156 123 742 85 1071 Cuts at: 5077 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 562 548 399 156 123 85 BspEI T'CCGG_A Cuts at: 3024 5523 3024 Size: 2499 3361 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 2133 96 Size: 2037 3823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3118 3920 4932 3118 Size: 802 1012 4046 Fragments arranged by size: 4046 1012 802 BstAPI GCAn_nnn'nTGC Cuts at: 794 2602 794 Size: 1808 4052 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2575 Size: 5860 BstYI r'GATC_y Cuts at: 1972 3096 3190 3557 3942 3959 5411 5497 5509 Size: 1124 94 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1124 385 367 346 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2363 2363 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2751 513 Size: 552 1686 3622 Fragments arranged by size: 3622 1686 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2698 2920 3051 487 Size: 683 56 1472 222 131 3296 Fragments arranged by size: 3296 1472 683 222 131 56 BtsI GCAGTG_nn' Cuts at: 3056 3376 4170 4190 3056 Size: 320 794 20 4726 Fragments arranged by size: 4726 794 320 20 ClaI AT'CG_AT Cuts at: 1554 2589 1554 Size: 1035 4825 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2786 4867 809 Size: 507 1470 2081 1802 Fragments arranged by size: 2081 1802 1470 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825 Size: 54 1425 50 275 18 1477 889 1629 Cuts at: 5825 8 Size: 43 Fragments arranged by size: 1629 1477 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2289 2480 3790 1353 Size: 819 117 191 1310 3423 Fragments arranged by size: 3423 1310 819 191 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3139 4447 4821 4967 387 Size: 1064 138 1550 1308 374 146 1280 Fragments arranged by size: 1550 1308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2176 2786 2909 2971 3083 3923 Size: 393 449 95 610 123 62 112 840 Cuts at: 3923 4520 5307 5575 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 840 787 610 597 449 393 268 123 112 95 62 EcoNI CCTnn'n_nnAGG Cuts at: 2362 2362 Size: 5860 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2021 5007 1678 Size: 343 2986 2531 Fragments arranged by size: 2986 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 2721 2753 2759 Size: 32 135 32 956 32 161 32 6 Cuts at: 2759 2791 2953 2985 3118 3150 4501 4533 4575 Size: 32 162 32 133 32 1351 32 42 Cuts at: 4575 4607 1373 Size: 32 2626 Fragments arranged by size: 2626 1351 956 162 161 135 133 42 32 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 2402 3185 2402 Size: 783 5077 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4038 Fragments arranged by size: 4038 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2264 2430 2810 3818 4177 4336 Size: 648 843 255 166 380 1008 359 159 Cuts at: 4336 518 Size: 2042 Fragments arranged by size: 2042 1008 843 648 380 359 255 166 159 MspA1I CmG'CkG Cuts at: 1606 2059 2525 2695 2959 3276 3953 5101 5346 Size: 453 466 170 264 317 677 1148 245 Cuts at: 5346 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 466 453 317 264 245 170 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2854 3218 4763 1132 Size: 97 1625 364 1545 2229 Fragments arranged by size: 2229 1625 1545 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 2850 4759 2850 Size: 1909 3951 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2763 2763 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2059 2525 2959 2059 Size: 466 434 4960 Fragments arranged by size: 4960 466 434 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 3208 4343 5024 5215 826 Size: 219 2163 1135 681 191 1471 Fragments arranged by size: 2163 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 3299 513 Size: 552 2234 3074 Fragments arranged by size: 3074 2234 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 2322 4106 Size: 274 80 33 51 1443 156 189 1784 Cuts at: 4106 5818 96 Size: 1712 138 Fragments arranged by size: 1784 1712 1443 274 189 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3035 3067 5820 5852 3035 Size: 32 2753 32 3043 Fragments arranged by size: 3043 2753 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 2298 2821 3989 2298 Size: 523 1168 4169 Fragments arranged by size: 4169 1168 523 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI