Restriction map for
pFN21AB9550
(Circular) (Six-base) MAPSORT of: pFN21AB9550.seq Check: 7805 from: 1 to: 5656
pFN21AB9550 5656 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 2999 1937
Size: 781 281 4594
Fragments arranged by size:
4594 781 281
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 2769 3783 4156 1563
Size: 1206 1014 373 3063
Fragments arranged by size:
3063 1206 1014 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 3719 5103 5371 1632
Size: 722 1365 1384 268 1917
Fragments arranged by size:
1917 1384 1365 722 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 2496 4555 5532 1203
Size: 1293 2059 977 1327
Fragments arranged by size:
2059 1327 1293 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2496 1184
Size: 1312 4344
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2053 2965 3191 3343 5452 1493
Size: 560 912 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 2977 4432 618
Size: 325 217 627 908 282 1455 1842
Fragments arranged by size:
1842 1455 908 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2217 2328 2457 2519 2975 1391
Size: 826 111 129 62 456 4072
Fragments arranged by size:
4072 826 456 129 111 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BbvCI CC'TCA_GC
Cuts at: 2829 2829
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 2878 1548
Size: 1330 4326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2284 4267 136
Size: 107 122 71 631 605 612 1983 1525
Fragments arranged by size:
1983 1525 631 612 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 3717 4873 1790
Size: 1927 1156 2573
Fragments arranged by size:
2573 1927 1156
BmgBI CAC'GTC
Cuts at: 1318 2497 2560 1318
Size: 1179 63 4414
Fragments arranged by size:
4414 1179 63
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2557 3387 4345
Size: 416 53 83 399 24 1140 830 958
Cuts at: 4345 442
Size: 1753
Fragments arranged by size:
1753 1140 958 830 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2342 2916 4316 1239
Size: 944 159 574 1400 2579
Fragments arranged by size:
2579 1400 944 574 159
Bpu10I CC'TnA_GC
Cuts at: 2829 2829
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2031 2171 2911 3781 4646 4944 2031
Size: 140 740 870 865 298 2743
Fragments arranged by size:
2743 870 865 740 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2260 4319 882
Size: 617 761 2059 2219
Fragments arranged by size:
2219 2059 761 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2914 2944 1178
Size: 30 165 30 1511 30 3890
Fragments arranged by size:
3890 1511 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2846 1186
Size: 1222 438 3996
Fragments arranged by size:
3996 1222 438
BseYI C'CCAG_C
Cuts at: 4859 5355 4859
Size: 496 5160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 1163
Size: 573 346 4737
Fragments arranged by size:
4737 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2827 2827
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2328 2457 2519 2975 3717 3802
Size: 399 427 111 129 62 456 742 85
Cuts at: 3802 4873 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 456 427 399 129 111
85 62
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2181 794
Size: 1387 4269
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2227 2622 2986 3353 3738 3755 5207 5293
Size: 255 395 364 367 385 17 1452 86
Cuts at: 5293 5305 5651 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 395 385 367 364 346 255
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 2435
Size: 5656
BtgI C'CryG_G
Cuts at: 513 1065 2218 2250 513
Size: 552 1153 32 3919
Fragments arranged by size:
3919 1153 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2608 2837 3172 3966 3986 2091
Size: 517 229 335 794 20 3761
Fragments arranged by size:
3761 794 517 335 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2623 2658 529
Size: 35 2059 35 3527
Fragments arranged by size:
3527 2059 35 35
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2588 4663 809
Size: 507 1272 2075 1802
Fragments arranged by size:
2075 1802 1272 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 3103 3992
Size: 54 1425 50 275 18 706 567 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 706 567 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 3586 1353
Size: 755 1478 3423
Fragments arranged by size:
3423 1478 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2342 2354 2916 3719 4316 5103
Size: 393 551 159 12 562 803 597 787
Cuts at: 5103 5371 1239
Size: 268 1524
Fragments arranged by size:
1524 803 787 597 562 551 393 268
159 12
EcoO109I rG'GnC_Cy
Cuts at: 2581 2581
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2163 2195 2884 2916 4297
Size: 32 135 32 591 32 689 32 1381
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1381 689 591 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 2629 2701 3072 3749 4897 5142 1606
Size: 515 508 72 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 508 371 245 72
NcoI C'CATG_G
Cuts at: 513 1065 2218 2250 513
Size: 552 1153 32 3919
Fragments arranged by size:
3919 1153 552 32
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2764 3014 4559 1132
Size: 97 1535 250 1545 2229
Fragments arranged by size:
2229 1545 1535 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2873 1108
Size: 1765 3891
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2589 2621 3725 3757 5238 5270 2589
Size: 32 1104 32 1481 32 2975
Fragments arranged by size:
2975 1481 1104 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2581 2581
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2208 2597 3008 830
Size: 1378 389 411 3478
Fragments arranged by size:
3478 1378 411 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2121 2629 2121
Size: 508 5148
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2204 2345 2593 3004 4139 4820 5011
Size: 219 1159 141 248 411 1135 681 191
Cuts at: 5011 826
Size: 1471
Fragments arranged by size:
1471 1159 1135 681 411 248 219 191
141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2046 2186 2890 3760 4661 4923
Size: 197 926 103 140 704 870 901 262
Cuts at: 4923 820
Size: 1553
Fragments arranged by size:
1553 926 901 870 704 262 197 140
103
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 2764 3014 1132
Size: 1632 250 3774
Fragments arranged by size:
3774 1632 250
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2218 2250 2357 2388 3095 513
Size: 552 1153 32 107 31 707 3074
Fragments arranged by size:
3074 1153 707 552 107 32 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 3902 5614
Size: 274 80 33 51 1443 92 1833 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1833 1712 1443 274 138 92 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2604 3785 2604
Size: 1181 4475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI