(Circular) (Six-base) MAPSORT of: pFN21AB9544.seq Check: 1385 from: 1 to: 6508 pFN21AB9544 6508 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2318 3851 1937 Size: 381 1533 4594 Fragments arranged by size: 4594 1533 381 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 2107 4635 5008 1563 Size: 544 2528 373 3063 Fragments arranged by size: 3063 2528 544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3572 4571 5955 6223 1632 Size: 1185 755 999 1384 268 1917 Fragments arranged by size: 1917 1384 1185 999 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 3234 5407 6384 1203 Size: 2031 2173 977 1327 Fragments arranged by size: 2173 2031 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 3425 1166 Size: 2259 4249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6508 AlwNI CAG_nnn'CTG Cuts at: 3391 4251 5823 3391 Size: 860 1572 4076 Fragments arranged by size: 4076 1572 860 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2161 3817 4043 4195 6304 1493 Size: 668 1656 226 152 2109 1697 Fragments arranged by size: 2109 1697 1656 668 226 152 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3833 1870 Size: 73 745 1145 4545 Fragments arranged by size: 4545 1145 745 73 AvrII C'CTAG_G Cuts at: 2713 2713 Size: 6508 BamHI G'GATC_C Cuts at: 3838 4205 3838 Size: 367 6141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3829 5284 618 Size: 325 217 627 2042 1455 1842 Fragments arranged by size: 2042 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3567 3827 1391 Size: 2176 260 4072 Fragments arranged by size: 4072 2176 260 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5537 BbvCI CC'TCA_GC Cuts at: 3330 3330 Size: 6508 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 2050 1548 Size: 502 6006 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2799 5119 136 Size: 107 122 71 631 605 1127 2320 1525 Fragments arranged by size: 2320 1525 1127 631 605 122 107 71 BglII A'GATC_T Cuts at: 6503 6503 Size: 6508 BlpI GC'TnA_GC Cuts at: 3925 3925 Size: 6508 Bme1580I G_kGCm'C Cuts at: 1790 3700 4569 5725 1790 Size: 1910 869 1156 2573 Fragments arranged by size: 2573 1910 1156 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2403 2815 4239 Size: 416 53 83 399 24 986 412 1424 Cuts at: 4239 5197 442 Size: 958 1753 Fragments arranged by size: 1753 1424 986 958 416 412 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3132 5168 1239 Size: 1893 2036 2579 Fragments arranged by size: 2579 2036 1893 Bpu10I CC'TnA_GC Cuts at: 2151 3330 3608 2151 Size: 1179 278 5051 Fragments arranged by size: 5051 1179 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5171 882 Size: 617 3672 2219 Fragments arranged by size: 3672 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4299 493 Size: 711 3095 2702 Fragments arranged by size: 3095 2702 711 BsaBI GATnn'nnATC Cuts at: 2049 6502 2049 Size: 4453 2055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2814 4315 4697 5332 Size: 53 83 186 936 1281 1501 382 635 Cuts at: 5332 275 Size: 1451 Fragments arranged by size: 1501 1451 1281 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6283 Fragments arranged by size: 6283 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2642 3081 3225 1186 Size: 1044 412 439 144 4469 Fragments arranged by size: 4469 1044 439 412 144 BseYI C'CCAG_C Cuts at: 2915 3095 5711 6207 2915 Size: 180 2616 496 3216 Fragments arranged by size: 3216 2616 496 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5935 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3958 4719 5747 1540 Size: 275 186 1957 761 1028 2301 Fragments arranged by size: 2301 1957 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2445 4569 4654 5725 2445 Size: 2124 85 1071 3228 Fragments arranged by size: 3228 2124 1071 85 BsmI GAATG_Cn' Cuts at: 4019 4112 4019 Size: 93 6415 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 3567 3700 3827 4569 4654 5725 Size: 399 655 1122 133 127 742 85 1071 Cuts at: 5725 1391 Size: 2174 Fragments arranged by size: 2174 1122 1071 742 655 399 133 127 85 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 4397 6127 4397 Size: 1730 4778 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3863 844 Size: 3019 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4395 1394 Size: 429 2572 3507 Fragments arranged by size: 3507 2572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3783 5003 5177 65 Size: 1183 2535 1220 174 1396 Fragments arranged by size: 2535 1396 1220 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5152 5339 1700 Size: 110 3342 187 2869 Fragments arranged by size: 3342 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6508 BstBI TT'CG_AA Cuts at: 5312 5312 Size: 6508 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6508 BstXI CCAn_nnnn'nTGG Cuts at: 3155 3155 Size: 6508 BstYI r'GATC_y Cuts at: 1972 3838 4205 4590 4607 6059 6145 6157 6503 Size: 1866 367 385 17 1452 86 12 346 Cuts at: 6503 1972 Size: 1977 Fragments arranged by size: 1977 1866 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 2476 4024 4818 4838 2476 Size: 1548 794 20 4146 Fragments arranged by size: 4146 1548 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6473 DraI TTT'AAA Cuts at: 2574 3812 2574 Size: 1238 5270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 3955 4844 Size: 54 1425 50 275 18 253 1872 889 Cuts at: 4844 6473 8 Size: 1629 43 Fragments arranged by size: 1872 1629 1425 889 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3955 1537 Size: 275 2143 4090 Fragments arranged by size: 4090 2143 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2222 2419 2969 3180 3481 4438 1353 Size: 774 95 197 550 211 301 957 3423 Fragments arranged by size: 3423 957 774 550 301 211 197 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5095 5469 5615 387 Size: 1064 138 3506 374 146 1280 Fragments arranged by size: 3506 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2817 3132 3572 4571 5168 5955 6223 Size: 393 1185 315 440 999 597 787 268 Cuts at: 6223 1239 Size: 1524 Fragments arranged by size: 1524 1185 999 787 597 440 393 315 268 EcoRI G'AATT_C Cuts at: 2161 3817 4195 2161 Size: 1656 378 4474 Fragments arranged by size: 4474 1656 378 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 5655 1678 Size: 3977 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2483 2515 2615 Size: 32 135 32 683 32 196 32 100 Cuts at: 2615 2647 2879 2911 5149 5181 5223 5255 1373 Size: 32 232 32 2238 32 42 32 2626 Fragments arranged by size: 2626 2238 683 232 196 135 100 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3852 4098 669 Size: 988 281 1914 246 3079 Fragments arranged by size: 3079 1914 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5622 5806 1305 Size: 4317 184 2007 Fragments arranged by size: 4317 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4686 Fragments arranged by size: 4686 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3153 3425 3521 4466 4825 4984 518 Size: 648 1987 272 96 945 359 159 2042 Fragments arranged by size: 2042 1987 945 648 359 272 159 96 MspA1I CmG'CkG Cuts at: 1606 2081 3592 3924 4601 5749 5994 1606 Size: 475 1511 332 677 1148 245 2120 Fragments arranged by size: 2120 1511 1148 677 475 332 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 3238 3717 3866 5411 1132 Size: 97 2009 479 149 1545 2229 Fragments arranged by size: 2229 2009 1545 479 149 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6508 PciI A'CATG_T Cuts at: 3234 5407 3234 Size: 2173 4335 PflMI CCAn_nnn'nTGG Cuts at: 1108 3110 3140 1108 Size: 2002 30 4476 Fragments arranged by size: 4476 2002 30 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6508 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PstI C_TGCA'G Cuts at: 830 3396 3633 3860 830 Size: 2566 237 227 3478 Fragments arranged by size: 3478 2566 237 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2081 3592 2081 Size: 1511 4997 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SapI GCTCTTCn'nnn_ Cuts at: 2419 2969 2419 Size: 550 5958 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 3489 4756 6468 3489 Size: 1267 1712 3529 Fragments arranged by size: 3529 1712 1267 SexAI A'CCwGG_T Cuts at: 3246 3246 Size: 6508 SfcI C'TryA_G Cuts at: 826 1045 3392 3480 3629 3856 4991 5672 5863 Size: 219 2347 88 149 227 1135 681 191 Cuts at: 5863 826 Size: 1471 Fragments arranged by size: 2347 1471 1135 681 227 219 191 149 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3835 1872 Size: 1963 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4612 5513 5775 820 Size: 197 926 2669 901 262 1553 Fragments arranged by size: 2669 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6508 StyI C'CwwG_G Cuts at: 513 1065 2004 2713 3700 3708 3947 513 Size: 552 939 709 987 8 239 3074 Fragments arranged by size: 3074 987 939 709 552 239 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3529 4535 4552 4705 4890 5335 325 Size: 1255 1949 1006 17 153 185 445 1498 Fragments arranged by size: 1949 1498 1255 1006 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3487 4754 6466 Size: 274 80 33 51 1443 1510 1267 1712 Cuts at: 6466 96 Size: 138 Fragments arranged by size: 1712 1510 1443 1267 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2750 2782 3011 3043 6468 6500 2750 Size: 32 229 32 3425 32 2758 Fragments arranged by size: 3425 2758 229 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3624 1316 Size: 2308 4200 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 3833 1870 Size: 1963 4545 XmnI GAAnn'nnTTC Cuts at: 2871 4637 2871 Size: 1766 4742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI