(Linear) (Six-base) MAPSORT of: KIBB9451.seq Check: 6601 from: 1 to: 996 KIBB9451.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AfeI AGC'GCT Cuts at: 0 469 996 Size: 469 527 ApaI G_GGCC'C Cuts at: 0 860 996 Size: 860 136 AscI GG'CGCG_CC Cuts at: 0 863 996 Size: 863 133 AvaI C'yCGr_G Cuts at: 0 286 955 996 Size: 286 669 41 Fragments arranged by size: 669 286 41 BanI G'GyrC_C Cuts at: 0 4 157 555 648 996 Size: 4 153 398 93 348 Fragments arranged by size: 398 348 153 93 4 BanII G_rGCy'C Cuts at: 0 215 347 782 860 902 996 Size: 215 132 435 78 42 94 Fragments arranged by size: 435 215 132 94 78 42 BbeI G_GCGC'C Cuts at: 0 161 559 652 996 Size: 161 398 93 344 Fragments arranged by size: 398 344 161 93 BbvCI CC'TCA_GC Cuts at: 0 551 996 Size: 551 445 BglI GCCn_nnn'nGGC Cuts at: 0 289 382 616 811 838 996 Size: 289 93 234 195 27 158 Fragments arranged by size: 289 234 195 158 93 27 BlpI GC'TnA_GC Cuts at: 0 496 996 Size: 496 500 Bme1580I G_kGCm'C Cuts at: 0 9 860 996 Size: 9 851 136 Fragments arranged by size: 851 136 9 BmrI ACTGGGnnnn_n' Cuts at: 0 3 996 Size: 3 993 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 366 861 996 Size: 366 495 135 Fragments arranged by size: 495 366 135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 996 Size: 554 442 Bpu10I CC'TnA_GC Cuts at: 0 551 996 Size: 551 445 BsaI GGTCTCn'nnnn_ Cuts at: 0 439 754 996 Size: 439 315 242 Fragments arranged by size: 439 315 242 BsaAI yAC'GTr Cuts at: 0 114 996 Size: 114 882 BsaHI Gr'CG_yC Cuts at: 0 158 238 556 649 996 Size: 158 80 318 93 347 Fragments arranged by size: 347 318 158 93 80 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 181 223 381 673 691 790 808 823 Size: 181 42 158 292 18 99 18 15 Cuts at: 823 910 996 Size: 87 86 Fragments arranged by size: 292 181 158 99 87 86 42 18 18 15 BseYI C'CCAG_C Cuts at: 0 436 781 950 978 996 Size: 436 345 169 28 18 Fragments arranged by size: 436 345 169 28 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 981 996 Size: 981 15 BsiEI CG_ry'CG Cuts at: 0 146 197 221 254 272 362 386 608 Size: 146 51 24 33 18 90 24 222 Cuts at: 608 908 996 Size: 300 88 Fragments arranged by size: 300 222 146 90 88 51 33 24 24 18 Bsp1286I G_dGCh'C Cuts at: 0 9 215 347 782 860 902 996 Size: 9 206 132 435 78 42 94 Fragments arranged by size: 435 206 132 94 78 42 9 BsrBI CCG'CTC Cuts at: 0 230 500 996 Size: 230 270 496 Fragments arranged by size: 496 270 230 BsrFI r'CCGG_y Cuts at: 0 267 996 Size: 267 729 BssHII G'CGCG_C Cuts at: 0 863 996 Size: 863 133 BstXI CCAn_nnnn'nTGG Cuts at: 0 789 996 Size: 789 207 BtgI C'CryG_G Cuts at: 0 44 140 215 248 323 356 362 890 Size: 44 96 75 33 75 33 6 528 Cuts at: 890 902 996 Size: 12 94 Fragments arranged by size: 528 96 94 75 75 44 33 33 12 6 EaeI y'GGCC_r Cuts at: 0 108 143 194 218 251 269 359 383 Size: 108 35 51 24 33 18 90 24 Cuts at: 383 605 905 996 Size: 222 300 91 Fragments arranged by size: 300 222 108 91 90 51 35 33 24 24 18 EagI C'GGCC_G Cuts at: 0 143 194 218 251 269 359 383 605 Size: 143 51 24 33 18 90 24 222 Cuts at: 605 905 996 Size: 300 91 Fragments arranged by size: 300 222 143 91 90 51 33 24 24 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 837 996 Size: 837 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 996 Size: 554 442 EcoO109I rG'GnC_Cy Cuts at: 0 318 840 996 Size: 318 522 156 Fragments arranged by size: 522 318 156 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 486 996 Size: 486 510 FspI TGC'GCA Cuts at: 0 930 996 Size: 930 66 HaeII r_GCGC'y Cuts at: 0 161 191 471 559 652 827 996 Size: 161 30 280 88 93 175 169 Fragments arranged by size: 280 175 169 161 93 88 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 364 396 996 Size: 364 32 600 Fragments arranged by size: 600 364 32 KasI G'GCGC_C Cuts at: 0 157 555 648 996 Size: 157 398 93 348 Fragments arranged by size: 398 348 157 93 MscI TGG'CCA Cuts at: 0 110 996 Size: 110 886 MspA1I CmG'CkG Cuts at: 0 46 142 217 250 280 325 364 562 Size: 46 96 75 33 30 45 39 198 Cuts at: 562 601 646 670 727 892 904 982 996 Size: 39 45 24 57 165 12 78 14 Fragments arranged by size: 198 165 96 78 75 57 46 45 45 39 39 33 30 24 14 12 NaeI GCC'GGC Cuts at: 0 269 996 Size: 269 727 NarI GG'CG_CC Cuts at: 0 158 556 649 996 Size: 158 398 93 347 Fragments arranged by size: 398 347 158 93 NgoMIV G'CCGG_C Cuts at: 0 267 996 Size: 267 729 NotI GC'GGCC_GC Cuts at: 0 143 218 605 905 996 Size: 143 75 387 300 91 Fragments arranged by size: 387 300 143 91 75 PmlI CAC'GTG Cuts at: 0 114 996 Size: 114 882 PspOMI G'GGCC_C Cuts at: 0 856 996 Size: 856 140 PstI C_TGCA'G Cuts at: 0 176 996 Size: 176 820 PvuII CAG'CTG Cuts at: 0 601 996 Size: 601 395 SacII CC_GC'GG Cuts at: 0 47 143 218 251 326 365 893 905 Size: 47 96 75 33 75 39 528 12 Cuts at: 905 996 Size: 91 Fragments arranged by size: 528 96 91 75 75 47 39 33 12 SfcI C'TryA_G Cuts at: 0 172 996 Size: 172 824 SfiI GGCCn_nnn'nGGCC Cuts at: 0 382 811 996 Size: 382 429 185 Fragments arranged by size: 429 382 185 SfoI GGC'GCC Cuts at: 0 159 557 650 996 Size: 159 398 93 346 Fragments arranged by size: 398 346 159 93 StuI AGG'CCT Cuts at: 0 54 996 Size: 54 942 StyI C'CwwG_G Cuts at: 0 161 407 764 996 Size: 161 246 357 232 Fragments arranged by size: 357 246 232 161 XmnI GAAnn'nnTTC Cuts at: 0 487 996 Size: 487 509 Enzymes that do cut: AfeI ApaI AscI AvaI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI BsrFI BssHII BstXI BtgI EaeI EagI EciI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV NotI PmlI PspOMI PstI PvuII SacII SfcI SfiI SfoI StuI StyI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI