(Circular) (Six-base) MAPSORT of: pFN21AB9445.seq Check: 1762 from: 1 to: 6721 pFN21AB9445 6721 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 4784 6168 6436 1632 Size: 1167 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 1167 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6524 AflIII A'CryG_T Cuts at: 1203 5620 6597 1203 Size: 4417 977 1327 Fragments arranged by size: 4417 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6721 AlwNI CAG_nnn'CTG Cuts at: 2672 3269 4464 6036 2672 Size: 597 1195 1572 3357 Fragments arranged by size: 3357 1572 1195 597 ApaLI G'TGCA_C Cuts at: 4778 5934 4778 Size: 1156 5565 ApoI r'AATT_y Cuts at: 1493 2129 2244 2336 3174 3627 3998 4030 4256 Size: 636 115 92 838 453 371 32 226 Cuts at: 4256 4408 6517 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 838 636 453 371 226 152 115 92 32 AseI AT'TA_AT Cuts at: 160 1032 3116 5275 160 Size: 872 2084 2159 1606 Fragments arranged by size: 2159 2084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 4046 1870 Size: 73 2103 4545 Fragments arranged by size: 4545 2103 73 BamHI G'GATC_C Cuts at: 4051 4418 4051 Size: 367 6354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4042 5497 618 Size: 325 217 627 2255 1455 1842 Fragments arranged by size: 2255 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2140 4040 1391 Size: 749 1900 4072 Fragments arranged by size: 4072 1900 749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2127 3693 928 Size: 971 228 1566 3956 Fragments arranged by size: 3956 1566 971 228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3579 4613 5823 3579 Size: 1034 1210 4477 Fragments arranged by size: 4477 1210 1034 BclI T'GATC_A Cuts at: 1548 3448 1548 Size: 1900 4821 BfrBI ATG'CAT Cuts at: 3797 3797 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5332 136 Size: 107 122 71 631 605 3660 1525 Fragments arranged by size: 3660 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2232 6716 2232 Size: 4484 2237 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 4782 5938 1790 Size: 2992 1156 2573 Fragments arranged by size: 2992 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4452 5410 442 Size: 416 53 83 399 24 3035 958 1753 Fragments arranged by size: 3035 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2706 1056 Size: 1650 5071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3019 3864 5381 1239 Size: 1780 845 1517 2579 Fragments arranged by size: 2579 1780 1517 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3401 4846 5711 6009 3401 Size: 1445 865 298 4113 Fragments arranged by size: 4113 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5384 882 Size: 617 3885 2219 Fragments arranged by size: 3885 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4512 493 Size: 711 3308 2702 Fragments arranged by size: 3308 2702 711 BsaBI GATnn'nnATC Cuts at: 6715 6715 Size: 6721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4528 4910 5545 275 Size: 53 83 186 936 2995 382 635 1451 Fragments arranged by size: 2995 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5154 5552 5826 5973 6384 5154 Size: 398 274 147 411 5491 Fragments arranged by size: 5491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3551 3581 3635 3665 1178 Size: 30 165 30 2148 30 54 30 4234 Fragments arranged by size: 4234 2148 165 54 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3005 3008 3011 1186 Size: 1819 3 3 4896 Fragments arranged by size: 4896 1819 3 3 BseYI C'CCAG_C Cuts at: 2195 5924 6420 2195 Size: 3729 496 2496 Fragments arranged by size: 3729 2496 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 1163 Size: 573 1218 4930 Fragments arranged by size: 4930 1218 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3051 4171 4932 5960 1540 Size: 275 186 1050 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4782 4867 5938 4782 Size: 85 1071 5565 Fragments arranged by size: 5565 1071 85 BsmI GAATG_Cn' Cuts at: 4232 4325 4232 Size: 93 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 4040 4782 4867 5938 1391 Size: 399 350 1900 742 85 1071 2174 Fragments arranged by size: 2174 1900 1071 742 399 350 85 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 4610 6340 4610 Size: 1730 4991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3762 4076 844 Size: 2918 314 3489 Fragments arranged by size: 3489 2918 314 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3837 5216 5390 65 Size: 1183 2589 1379 174 1396 Fragments arranged by size: 2589 1396 1379 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 2699 794 Size: 1905 4816 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 2480 3921 2480 Size: 1441 5280 BstYI r'GATC_y Cuts at: 1972 2232 3730 4051 4418 4803 4820 6272 6358 Size: 260 1498 321 367 385 17 1452 86 Cuts at: 6358 6370 6716 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1498 1452 385 367 346 321 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2086 513 Size: 552 939 82 5148 Fragments arranged by size: 5148 939 552 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 4237 5031 5051 4237 Size: 794 20 5907 Fragments arranged by size: 5907 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 3964 4025 3964 Size: 61 6660 DraIII CAC_nnn'GTG Cuts at: 2699 2699 Size: 6721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3050 5728 809 Size: 507 1734 2678 1802 Fragments arranged by size: 2678 1802 1734 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 3048 4168 Size: 54 1425 50 275 18 253 965 1120 Cuts at: 4168 5057 6686 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1120 965 889 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3048 4168 1537 Size: 275 1236 1120 4090 Fragments arranged by size: 4090 1236 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2189 2920 2982 4651 1353 Size: 746 90 731 62 1669 3423 Fragments arranged by size: 3423 1669 746 731 90 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5308 5682 5828 387 Size: 1064 138 3719 374 146 1280 Fragments arranged by size: 3719 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2799 3019 3864 4784 5381 6168 6436 Size: 393 1167 220 845 920 597 787 268 Cuts at: 6436 1239 Size: 1524 Fragments arranged by size: 1524 1167 920 845 787 597 393 268 220 EcoNI CCTnn'n_nnAGG Cuts at: 3363 3882 3363 Size: 519 6202 EcoO109I rG'GnC_Cy Cuts at: 2669 2669 Size: 6721 EcoRI G'AATT_C Cuts at: 4030 4408 4030 Size: 378 6343 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3173 3173 Size: 6721 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 5868 1678 Size: 4190 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 5362 5394 5436 Size: 32 135 32 645 32 3113 32 42 Cuts at: 5436 5468 1373 Size: 32 2626 Fragments arranged by size: 3113 2626 645 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3862 4065 4311 669 Size: 988 281 1924 203 246 3079 Fragments arranged by size: 3079 1924 988 281 246 203 HindIII A'AGCT_T Cuts at: 748 3526 3749 748 Size: 2778 223 3720 Fragments arranged by size: 3720 2778 223 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 4320 4320 Size: 6721 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2846 5835 6019 1305 Size: 1541 2989 184 2007 Fragments arranged by size: 2989 2007 1541 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2085 10 Size: 54 1425 343 253 4646 Fragments arranged by size: 4646 1425 343 253 54 MslI CAynn'nnrTG Cuts at: 518 1166 4679 5038 5197 518 Size: 648 3513 359 159 2042 Fragments arranged by size: 3513 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4137 4814 5962 6207 1606 Size: 2531 677 1148 245 2120 Fragments arranged by size: 2531 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2086 513 Size: 552 939 82 5148 Fragments arranged by size: 5148 939 552 82 NdeI CA'TA_TG Cuts at: 387 2907 3511 387 Size: 2520 604 3597 Fragments arranged by size: 3597 2520 604 NheI G'CTAG_C Cuts at: 1052 2702 1052 Size: 1650 5071 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NsiI A_TGCA'T Cuts at: 3799 3799 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 4079 5624 1132 Size: 97 2850 1545 2229 Fragments arranged by size: 2850 2229 1545 97 PacI TTA_AT'TAA Cuts at: 2310 2310 Size: 6721 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6721 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4790 4822 6303 6335 4790 Size: 32 1481 32 5176 Fragments arranged by size: 5176 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6721 PsiI TTA'TAA Cuts at: 2222 2504 4004 4291 2222 Size: 282 1500 287 4652 Fragments arranged by size: 4652 1500 287 282 PstI C_TGCA'G Cuts at: 830 4073 830 Size: 3243 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SapI GCTCTTCn'nnn_ Cuts at: 2189 2189 Size: 6721 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 2045 3988 4969 6681 2045 Size: 1943 981 1712 2085 Fragments arranged by size: 2085 1943 1712 981 SfcI C'TryA_G Cuts at: 826 1045 2031 2260 4069 5204 5885 6076 826 Size: 219 986 229 1809 1135 681 191 1471 Fragments arranged by size: 1809 1471 1135 986 681 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 4048 1872 Size: 2176 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3380 4825 5726 5988 820 Size: 197 926 1437 1445 901 262 1553 Fragments arranged by size: 1553 1445 1437 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 4079 1132 Size: 2947 3774 SspI AAT'ATT Cuts at: 5 52 2149 2842 4645 5 Size: 47 2097 693 1803 2081 Fragments arranged by size: 2097 2081 1803 693 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6721 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 4160 513 Size: 552 939 82 2074 3074 Fragments arranged by size: 3074 2074 939 552 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 4748 4765 4918 5103 5548 325 Size: 1255 559 2609 17 153 185 445 1498 Fragments arranged by size: 2609 1498 1255 559 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2043 3986 4967 Size: 274 80 33 51 1443 66 1943 981 Cuts at: 4967 6679 96 Size: 1712 138 Fragments arranged by size: 1943 1712 1443 981 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6681 6713 6681 Size: 32 6689 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 4057 4057 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 4046 1870 Size: 2176 4545 XmnI GAAnn'nnTTC Cuts at: 4850 4850 Size: 6721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI