Restriction map for
pFN21AB9434
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII HinfI HpaI HphI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tru9I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 58 AAATTT [GA]^AATT[CT]
Aor51HI 330 AGCGCT AGC^GCT
ApaI 276 GGGCCC GGGCC^C
ApoI 58 AAATTT [GA]^AATT[CT]
Asp700I 397 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 117 CTCGGG C^[CT]CG[GA]G
BanI 226 GGCACC G^G[CT][GA]CC
BfaI 610 CTAG C^TAG
BglII 446 AGATCT A^GATCT
BsiEI 510 CGACCG CG[GA][CT]^CG
BsoBI 117 CTCGGG C^[CT]CG[GA]G
BsrBI 250 GAGCGG CCGCTC(-3/-3)
BstNI 67 CCTGG CC^[AT]GG
Cac8I 336 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 331 GCGC GCG^C
DdeI 101 CTCAG C^T[ACGT]AG
Eco47III 330 AGCGCT AGC^GCT
EcoNI 12 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 67 CCTGG ^CC[AT]GG
HaeII 330 AGCGCT [GA]GCGC^[CT]
HhaI 331 GCGC GCG^C
HinP1I 331 GCGC G^CGC
HincII 544 GTCAAC GT[CT]^[GA]AC
HindII 544 GTCAAC GT[CT]^[GA]AC
MaeI 610 CTAG C^TAG
MaeII 491 ACGT A^CGT
MvaI 67 CCTGG CC^[AT]GG
PflFI 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfaNI 106 GCATC GCATC(5/9)
SmlI 310 CTCAAG C^T[CT][GA]AG
SspI 399 AATATT AAT^ATT
TaqI 509 TCGA T^CGA
Tsp509I 59 AATT ^AATT
TspRI 153 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 511 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 509 TCGA T^CGA
XmnI 397 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 184 GTAC GT^AC
348 GTAC
AflIII 186 ACATGT A^C[GA][CT]GT
409 ACATGT
AlwI 459 GATCC GGATC(4/5)
209 GGATC
BanII 98 GGGCTC G[GA]GC[CT]^C
276 GGGCCC
BbvI 87 GCTGC GCAGC(8/12)
333 GCTGC
BcnI 477 CCGGG CC^[GC]GG
513 CCGGG
BmyI 98 GGGCTC G[AGT]GC[ACT]^C
276 GGGCCC
Bsp1286I 98 GGGCTC G[AGT]GC[ACT]^C
276 GGGCCC
BspLU11I 186 ACATGT A^CATGT
409 ACATGT
BspMI 550 ACCTGC ACCTGC(4/8)
18 GCAGGT
BstYI 446 AGATCT [GA]^GATC[CT]
458 AGATCC
DraII 275 GGGGCCC [GA]G^G[ACGT]CC[CT]
276 GGGCCCC
EcoO109I 275 GGGGCCC [GA]G^G[ACGT]CC[CT]
276 GGGCCCC
HaeIII 277 GGCC GG^CC
537 GGCC
HapII 477 CCGG C^CGG
513 CCGG
HpaII 477 CCGG C^CGG
513 CCGG
MaeIII 577 GTCAC ^GT[ACGT]AC
644 GTCAC
MboII 456 GAAGA GAAGA(8/7)
200 TCTTC
MflI 446 AGATCT [GA]^GATC[CT]
458 AGATCC
MslI 519 CATTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
579 CACAGAGATG
MspI 477 CCGG C^CGG
513 CCGG
MwoI 324 GCCAACAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
331 GCGCTGCCAGC
NciI 477 CCGGG CC^[GC]GG
513 CCGGG
NspI 186 ACATGT [GA]CATG^[CT]
409 ACATGT
RsaI 184 GTAC GT^AC
348 GTAC
TseI 87 GCTGC G^C[AT]GC
333 GCTGC
Tsp45I 577 GTCAC ^GT[GC]AC
644 GTCAC
XhoII 446 AGATCT [GA]^GATC[CT]
458 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AvaII BsgI BsiYI
BslI BsrI BssKI BstF5I Cfr13I
DpnII Fnu4HI FokI ItaI MboI
MnlI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI