(Circular) (Six-base) MAPSORT of: pFN21AB9273.seq Check: 8763 from: 1 to: 5833 pFN21AB9273 5833 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 3176 1937 Size: 852 387 4594 Fragments arranged by size: 4594 852 387 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2690 3896 5280 5548 1632 Size: 995 63 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 995 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2868 4732 5709 1203 Size: 1665 1864 977 1327 Fragments arranged by size: 1864 1665 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2958 3040 1184 Size: 1774 82 3977 Fragments arranged by size: 3977 1774 82 AlwNI CAG_nnn'CTG Cuts at: 2713 3576 5148 2713 Size: 863 1572 3398 Fragments arranged by size: 3398 1572 863 ApaLI G'TGCA_C Cuts at: 2933 3890 5046 2933 Size: 957 1156 3720 Fragments arranged by size: 3720 1156 957 ApoI r'AATT_y Cuts at: 1493 2321 2686 3142 3368 3520 5629 1493 Size: 828 365 456 226 152 2109 1697 Fragments arranged by size: 2109 1697 828 456 365 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 2832 3152 1391 Size: 1441 320 4072 Fragments arranged by size: 4072 1441 320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2754 928 Size: 971 855 4007 Fragments arranged by size: 4007 971 855 BbvCI CC'TCA_GC Cuts at: 2075 2918 2075 Size: 843 4990 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4024 4058 1903 Size: 34 50 34 2003 34 3678 Fragments arranged by size: 3678 2003 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2449 4444 Size: 107 122 71 631 605 337 440 1995 Cuts at: 4444 136 Size: 1525 Fragments arranged by size: 1995 1525 631 605 440 337 122 107 71 BglII A'GATC_T Cuts at: 2190 5828 2190 Size: 3638 2195 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2937 3894 5050 1790 Size: 1147 957 1156 2573 Fragments arranged by size: 2573 1156 1147 957 BmgBI CAC'GTC Cuts at: 1318 2023 2869 1318 Size: 705 846 4282 Fragments arranged by size: 4282 846 705 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 3564 4522 Size: 416 53 83 399 24 711 1436 958 Cuts at: 4522 442 Size: 1753 Fragments arranged by size: 1753 1436 958 711 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 3084 4493 1239 Size: 1241 604 1409 2579 Fragments arranged by size: 2579 1409 1241 604 Bpu10I CC'TnA_GC Cuts at: 2075 2918 2075 Size: 843 4990 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 2839 3624 493 Size: 711 1020 615 785 2702 Fragments arranged by size: 2702 1020 785 711 615 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 2575 2963 1186 Size: 1297 92 388 4056 Fragments arranged by size: 4056 1297 388 92 BseYI C'CCAG_C Cuts at: 2896 3077 3120 5036 5532 2896 Size: 181 43 1916 496 3197 Fragments arranged by size: 3197 1916 496 181 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2937 3894 3979 5050 2937 Size: 957 85 1071 3720 Fragments arranged by size: 3720 1071 957 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2832 2937 3152 3894 3979 5050 1391 Size: 399 1042 105 215 742 85 1071 2174 Fragments arranged by size: 2174 1071 1042 742 399 215 105 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3188 844 Size: 2078 266 3489 Fragments arranged by size: 3489 2078 266 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2370 4328 4502 65 Size: 1183 1122 1958 174 1396 Fragments arranged by size: 1958 1396 1183 1122 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 2904 96 Size: 2808 3025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 2014 794 Size: 1220 4613 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 2087 1184 Size: 903 4930 BstYI r'GATC_y Cuts at: 1972 2190 3067 3163 3530 3915 3932 5384 5470 Size: 218 877 96 367 385 17 1452 86 Cuts at: 5470 5482 5828 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 877 385 367 346 218 96 86 17 12 BstZ17I GTA'TAC Cuts at: 2790 2790 Size: 5833 Bsu36I CC'TnA_GG Cuts at: 2558 2558 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 2058 513 Size: 552 993 4288 Fragments arranged by size: 4288 993 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 2515 3137 2515 Size: 622 5211 DraIII CAC_nnn'GTG Cuts at: 2026 2026 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 3127 3280 Size: 54 1425 50 275 18 231 1066 153 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1066 889 275 231 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2675 3763 1353 Size: 1322 1088 3423 Fragments arranged by size: 3423 1322 1088 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 2993 4420 4794 4940 387 Size: 1064 138 506 898 1427 374 146 1280 Fragments arranged by size: 1427 1280 1064 898 506 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2627 2690 3084 3896 4493 5280 Size: 393 848 147 63 394 812 597 787 Cuts at: 5280 5548 1239 Size: 268 1524 Fragments arranged by size: 1524 848 812 787 597 394 393 268 147 63 EcoO109I rG'GnC_Cy Cuts at: 2911 2911 Size: 5833 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2058 4980 1678 Size: 380 2922 2531 Fragments arranged by size: 2922 2531 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2275 2307 4474 4506 4548 Size: 32 135 32 703 32 2167 32 42 Cuts at: 4548 4580 1373 Size: 32 2626 Fragments arranged by size: 2626 2167 703 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4947 5131 1305 Size: 3642 184 2007 Fragments arranged by size: 3642 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3129 10 Size: 54 1425 343 1297 2714 Fragments arranged by size: 2714 1425 1297 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 3791 4150 4309 518 Size: 648 1497 1128 359 159 2042 Fragments arranged by size: 2042 1497 1128 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2504 3061 3249 3926 5074 5319 1606 Size: 898 557 188 677 1148 245 2120 Fragments arranged by size: 2120 1148 898 677 557 245 188 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2668 3191 4736 1132 Size: 97 1439 523 1545 2229 Fragments arranged by size: 2229 1545 1439 523 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 3013 1108 Size: 1905 3928 PfoI T'CCnGG_A Cuts at: 2970 2970 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 3902 3934 5415 5447 2108 Size: 32 1762 32 1481 32 2494 Fragments arranged by size: 2494 1762 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 2911 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 2718 3185 830 Size: 1888 467 3478 Fragments arranged by size: 3478 1888 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2504 2504 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SexAI A'CCwGG_T Cuts at: 2091 2725 2091 Size: 634 5199 SfcI C'TryA_G Cuts at: 826 1045 2714 3181 4316 4997 5188 826 Size: 219 1669 467 1135 681 191 1471 Fragments arranged by size: 1669 1471 1135 681 467 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2224 3624 493 Size: 1731 1400 2702 Fragments arranged by size: 2702 1731 1400 SpeI A'CTAG_T Cuts at: 152 3019 152 Size: 2867 2966 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 2012 1517 Size: 495 5338 StyI C'CwwG_G Cuts at: 513 1065 2793 3272 513 Size: 552 1728 479 3074 Fragments arranged by size: 3074 1728 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2910 3860 3877 4030 4215 4660 325 Size: 1255 1330 950 17 153 185 445 1498 Fragments arranged by size: 1498 1330 1255 950 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2147 2904 4079 Size: 274 80 33 51 1443 170 757 1175 Cuts at: 4079 5791 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1175 757 274 170 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2723 2755 5793 5825 2723 Size: 32 3038 32 2731 Fragments arranged by size: 3038 2731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2727 2855 1316 Size: 1411 128 4294 Fragments arranged by size: 4294 1411 128 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 3117 2575 Size: 542 5291 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI