(Circular) (Six-base) MAPSORT of: pFN21AB8994.seq Check: 1821 from: 1 to: 5692 pFN21AB8994 5692 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2158 2158 Size: 5692 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 2976 3035 1937 Size: 328 711 59 4594 Fragments arranged by size: 4594 711 328 59 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 2991 3819 4192 1563 Size: 1428 828 373 3063 Fragments arranged by size: 3063 1428 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3755 5139 5407 1632 Size: 2123 1384 268 1917 Fragments arranged by size: 2123 1917 1384 268 AfeI AGC'GCT Cuts at: 2330 2858 2330 Size: 528 5164 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2905 3435 5007 2905 Size: 530 1572 3590 Fragments arranged by size: 3590 1572 530 ApaI G_GGCC'C Cuts at: 2653 2718 2653 Size: 65 5627 ApaLI G'TGCA_C Cuts at: 2318 3749 4905 2318 Size: 1431 1156 3105 Fragments arranged by size: 3105 1431 1156 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2646 2922 3017 1870 Size: 73 703 276 95 4545 Fragments arranged by size: 4545 703 276 95 73 AvrII C'CTAG_G Cuts at: 2803 2803 Size: 5692 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2456 2892 3013 4468 Size: 325 217 627 242 427 436 121 1455 Cuts at: 4468 618 Size: 1842 Fragments arranged by size: 1842 1455 627 436 427 325 242 217 121 BanII G_rGCy'C Cuts at: 1391 2064 2520 2634 2653 2718 3011 1391 Size: 673 456 114 19 65 293 4072 Fragments arranged by size: 4072 673 456 293 114 65 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4721 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2773 4303 136 Size: 107 122 71 631 605 1101 1530 1525 Fragments arranged by size: 1530 1525 1101 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2034 2322 2653 2677 2718 2832 3753 4909 Size: 244 288 331 24 41 114 921 1156 Cuts at: 4909 1790 Size: 2573 Fragments arranged by size: 2573 1156 921 331 288 244 114 41 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2780 3423 4381 Size: 416 53 83 399 24 1363 643 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1363 958 643 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2627 2680 2703 2893 4352 1239 Size: 1100 288 53 23 190 1459 2579 Fragments arranged by size: 2579 1459 1100 288 190 53 23 Bpu10I CC'TnA_GC Cuts at: 2168 2168 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 3483 493 Size: 711 1518 761 2702 Fragments arranged by size: 2702 1518 761 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3499 3881 4516 Size: 53 83 186 936 476 1490 382 635 Cuts at: 4516 275 Size: 1451 Fragments arranged by size: 1490 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4387 Fragments arranged by size: 4387 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2378 2726 2786 1186 Size: 1063 129 348 60 4092 Fragments arranged by size: 4092 1063 348 129 60 BseYI C'CCAG_C Cuts at: 2033 2183 2485 2709 2845 2939 4895 5391 2033 Size: 150 302 224 136 94 1956 496 2334 Fragments arranged by size: 2334 1956 496 302 224 150 136 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2838 1163 Size: 573 1102 4017 Fragments arranged by size: 4017 1102 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 3753 3838 4909 2322 Size: 1431 85 1071 3105 Fragments arranged by size: 3105 1431 1071 85 BsmI GAATG_Cn' Cuts at: 2370 3203 3296 2370 Size: 833 93 4766 Fragments arranged by size: 4766 833 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2064 2322 2520 2634 2653 2677 Size: 399 244 30 258 198 114 19 24 Cuts at: 2677 2718 2832 3011 3753 3838 4909 1391 Size: 41 114 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 258 244 198 179 114 114 85 41 30 24 19 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2158 3047 844 Size: 1265 49 889 3489 Fragments arranged by size: 3489 1265 889 49 BsrBI CCG'CTC Cuts at: 1394 1823 2429 2611 3579 1394 Size: 429 606 182 968 3507 Fragments arranged by size: 3507 968 606 429 182 BsrDI GCAATG_nn' Cuts at: 65 1248 2204 2210 2943 4187 4361 65 Size: 1183 956 6 733 1244 174 1396 Fragments arranged by size: 1396 1244 1183 956 733 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 2601 4336 4523 1700 Size: 110 791 1735 187 2869 Fragments arranged by size: 2869 1735 791 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2223 2363 2845 2223 Size: 140 482 5070 Fragments arranged by size: 5070 482 140 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2345 2345 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 2532 2838 513 Size: 552 1467 306 3367 Fragments arranged by size: 3367 1467 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2104 2640 487 Size: 683 56 878 536 3539 Fragments arranged by size: 3539 878 683 536 56 BtsI GCAGTG_nn' Cuts at: 2571 2622 2729 2832 2977 3208 4002 4022 2571 Size: 51 107 103 145 231 794 20 4241 Fragments arranged by size: 4241 794 231 145 107 103 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2788 4699 809 Size: 507 1472 1911 1802 Fragments arranged by size: 1911 1802 1472 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 2724 3139 Size: 54 1425 50 275 18 738 156 415 Cuts at: 3139 4028 5657 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 738 415 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2702 2908 3622 1353 Size: 1349 206 714 3423 Fragments arranged by size: 3423 1349 714 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2626 4279 4653 4799 387 Size: 1064 138 1037 1653 374 146 1280 Fragments arranged by size: 1653 1280 1064 1037 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2627 2680 2703 2893 3755 4352 Size: 393 707 288 53 23 190 862 597 Cuts at: 4352 5139 5407 1239 Size: 787 268 1524 Fragments arranged by size: 1524 862 787 707 597 393 288 268 190 53 23 EcoO109I rG'GnC_Cy Cuts at: 2650 2714 2715 2774 2650 Size: 64 1 59 5568 Fragments arranged by size: 5568 64 59 1 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 2141 4198 2141 Size: 2057 3635 HaeII r_GCGC'y Cuts at: 1678 2332 2764 2860 4839 1678 Size: 654 432 96 1979 2531 Fragments arranged by size: 2531 1979 654 432 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 4333 4365 4407 Size: 32 135 32 1046 32 1683 32 42 Cuts at: 4407 4439 1373 Size: 32 2626 Fragments arranged by size: 2626 1683 1046 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2977 3036 3282 669 Size: 988 281 1039 59 246 3079 Fragments arranged by size: 3079 1039 988 281 246 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 2726 10 Size: 54 1425 343 738 156 2976 Fragments arranged by size: 2976 1425 738 343 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 3650 4009 4168 518 Size: 648 2484 359 159 2042 Fragments arranged by size: 2484 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2587 2822 3108 3785 4933 5178 1606 Size: 981 235 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 677 286 245 235 NaeI GCC'GGC Cuts at: 2603 2603 Size: 5692 NcoI C'CATG_G Cuts at: 513 1065 2838 513 Size: 552 1773 3367 Fragments arranged by size: 3367 1773 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NgoMIV G'CCGG_C Cuts at: 2601 2601 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 2113 2363 1348 Size: 765 250 4677 Fragments arranged by size: 4677 765 250 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 2153 1108 Size: 1045 4647 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2649 2714 2649 Size: 65 5627 PstI C_TGCA'G Cuts at: 830 2468 2592 2901 3044 830 Size: 1638 124 309 143 3478 Fragments arranged by size: 3478 1638 309 143 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2587 2587 Size: 5692 SalI G'TCGA_C Cuts at: 1936 2975 3034 1936 Size: 1039 59 4594 Fragments arranged by size: 4594 1039 59 SapI GCTCTTCn'nnn_ Cuts at: 2702 2908 2702 Size: 206 5486 SbfI CC_TGCA'GG Cuts at: 2901 3044 2901 Size: 143 5549 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2464 2588 2897 3040 4175 4856 5047 Size: 219 1419 124 309 143 1135 681 191 Cuts at: 5047 826 Size: 1471 Fragments arranged by size: 1471 1419 1135 681 309 219 191 143 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 2648 2924 3019 1872 Size: 776 276 95 4545 Fragments arranged by size: 4545 776 276 95 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SrfI GCCC'GGGC Cuts at: 2648 2648 Size: 5692 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2046 2803 2838 3131 513 Size: 552 981 757 35 293 3074 Fragments arranged by size: 3074 981 757 552 293 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2525 2970 3719 3736 3889 4074 4519 Size: 1255 945 445 749 17 153 185 445 Cuts at: 4519 325 Size: 1498 Fragments arranged by size: 1498 1255 945 749 445 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2440 2472 5652 5684 2440 Size: 32 3180 32 2448 Fragments arranged by size: 3180 2448 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2311 2450 2053 Size: 258 139 5295 Fragments arranged by size: 5295 258 139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 2646 2922 3017 1870 Size: 776 276 95 4545 Fragments arranged by size: 4545 776 276 95 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI