Restriction map for
pFN21AB8988
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MseI MslI
MspA1I MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XhoII
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 991 GAATTC [GA]^AATT[CT]
AflIII 584 ACACGT A^C[GA][CT]GT
ApoI 991 GAATTC [GA]^AATT[CT]
Asp700I 147 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 258 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 986 GCAATGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 131 GTATCC GTATCC(6/5)
BlpI 222 GCTCAGC GC^T[ACGT]AGC
BpmI 637 CTGGAG CTGGAG(16/14)
Bpu1102I 222 GCTCAGC GC^T[ACGT]AGC
BsmBI 801 CGTCTC CGTCTC(1/5)
BsrBI 295 GAGCGG CCGCTC(-3/-3)
BsrDI 986 GCAATG GCAATG(2/0)
BssSI 535 CACGAG CACGAG(-5/-1)
BstAPI 309 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 222 GCTCAGC GC^T[ACGT]AGC
DraII 1089 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1089 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 991 GAATTC G^AATTC
HinfI 1027 GACTC G^A[ACGT]TC
MaeIII 1078 GTGAC ^GT[ACGT]AC
NcoI 1043 CCATGG C^CATGG
PaeR7I 40 CTCGAG C^TCGAG
PflFI 258 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 762 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1027 GACTC GAGTC(4/5)
PpuMI 1089 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 307 CTGCAG CTGCA^G
ScaI 491 AGTACT AGT^ACT
SmlI 40 CTCGAG C^T[CT][GA]AG
Tsp45I 1078 GTGAC ^GT[GC]AC
Tsp509I 992 AATT ^AATT
Tth111I 258 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 762 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 40 CTCGAG C^TCGAG
XmnI 147 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 347 CGCG CG^CG
382 CGCG
AlwI 945 GGATC GGATC(4/5)
981 GGATC
BanI 565 GGCACC G^G[CT][GA]CC
716 GGCACC
BglI 518 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1033 GCCACAGGGGC
BsaWI 729 ACCGGA [AT]^CCGG[AT]
1065 TCCGGT
BseRI 660 CTCCTC GAGGAG(10/8)
676 GAGGAG
BsgI 208 CTGCAC GTGCAG(16/14)
193 GTGCAG
BsiEI 89 CGGCCG CG[GA][CT]^CG
343 CGGCCG
BsiWI 278 CGTACG C^GTACG
593 CGTACG
BspMI 206 ACCTGC ACCTGC(4/8)
707 ACCTGC
BsrI 789 CCAGT ACTGG(1/-1)
999 CCAGT
BstUI 347 CGCG CG^CG
382 CGCG
DpnII 946 GATC ^GATC
982 GATC
DsaI 1043 CCATGG C^C[GA][CT]GG
1130 CCACGG
EagI 89 CGGCCG C^GGCCG
343 CGGCCG
EarI 270 GAAGAG CTCTTC(1/4)
441 GAAGAG
EclXI 89 CGGCCG C^GGCCG
343 CGGCCG
Eco52I 89 CGGCCG C^GGCCG
343 CGGCCG
FauI 692 CCCGC CCCGC(4/6)
912 CCCGC
HaeII 252 GGCGCT [GA]GCGC^[CT]
462 GGCGCT
Ksp632I 270 GAAGAG CTCTTC(1/4)
441 GAAGAG
MboI 946 GATC ^GATC
982 GATC
MvnI 347 CGCG CG^CG
382 CGCG
NaeI 87 GCCGGC GCC^GGC
543 GCCGGC
NdeII 946 GATC ^GATC
982 GATC
NgoMIV 87 GCCGGC G^CCGGC
543 GCCGGC
NspI 314 ACATGC [GA]CATG^[CT]
1059 GCATGT
SacI 220 GAGCTC GAGCT^C
405 GAGCTC
Sau3AI 946 GATC ^GATC
982 GATC
XcmI 672 CCAGGAGGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1034 CCACAGGGGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BalI BanII BbvI
BcnI BmyI BsaAI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI TspRI TthHB8I XmaCI XmaI