(Circular) (Six-base) MAPSORT of: pFN21AB8988.seq Check: 1855 from: 1 to: 5869 pFN21AB8988 5869 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2721 2721 Size: 5869 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2589 4768 5745 1203 Size: 1386 2179 977 1327 Fragments arranged by size: 2179 1386 1327 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2531 1184 Size: 1347 4522 AlwNI CAG_nnn'CTG Cuts at: 3612 5184 3612 Size: 1572 4297 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 2996 3178 3404 3556 5665 1493 Size: 1503 182 226 152 2109 1697 Fragments arranged by size: 2109 1697 1503 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2045 2110 2395 2421 2575 3194 Size: 73 93 9 65 285 26 154 619 Cuts at: 3194 1870 Size: 4545 Fragments arranged by size: 4545 619 285 154 93 73 65 26 9 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2570 2721 3190 4645 618 Size: 325 217 627 783 151 469 1455 1842 Fragments arranged by size: 1842 1455 783 627 469 325 217 151 BanII G_rGCy'C Cuts at: 1391 2126 2229 2414 2780 3188 1391 Size: 735 103 185 366 408 4072 Fragments arranged by size: 4072 735 408 366 185 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2980 3014 4060 4094 1903 Size: 34 1043 34 1046 34 3678 Fragments arranged by size: 3678 1046 1043 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2147 3761 4971 2147 Size: 1614 1210 3045 Fragments arranged by size: 3045 1614 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2529 3044 4480 Size: 107 122 71 631 605 857 515 1436 Cuts at: 4480 136 Size: 1525 Fragments arranged by size: 1525 1436 857 631 605 515 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 2228 3286 2228 Size: 1058 4811 Bme1580I G_kGCm'C Cuts at: 1790 2054 2724 3930 5086 1790 Size: 264 670 1206 1156 2573 Fragments arranged by size: 2573 1206 1156 670 264 BmgBI CAC'GTC Cuts at: 1318 2638 1318 Size: 1320 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3600 4558 442 Size: 416 53 83 399 24 2183 958 1753 Fragments arranged by size: 2183 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 4529 1239 Size: 1424 1866 2579 Fragments arranged by size: 2579 1866 1424 Bpu10I CC'TnA_GC Cuts at: 2925 2925 Size: 5869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3994 4859 5157 3994 Size: 865 298 4706 Fragments arranged by size: 4706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 3079 3098 4532 882 Size: 617 549 1031 19 1434 2219 Fragments arranged by size: 2219 1434 1031 617 549 19 BsaAI yAC'GTr Cuts at: 493 1204 2287 2592 3144 3660 493 Size: 711 1083 305 552 516 2702 Fragments arranged by size: 2702 1083 711 552 516 305 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2805 2943 3676 Size: 53 83 186 936 531 741 138 733 Cuts at: 3676 4058 4693 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 741 733 635 531 382 186 138 83 53 BsaWI w'CCGG_w Cuts at: 2734 3070 4302 4700 4974 5121 5532 2734 Size: 336 1232 398 274 147 411 3071 Fragments arranged by size: 3071 1232 411 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 3017 3047 1178 Size: 30 165 30 705 30 879 30 4000 Fragments arranged by size: 4000 879 705 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2656 2696 1186 Size: 1470 40 4359 Fragments arranged by size: 4359 1470 40 BseYI C'CCAG_C Cuts at: 2017 2239 2470 2519 5072 5568 2017 Size: 222 231 49 2553 496 2318 Fragments arranged by size: 2553 2318 496 231 222 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2219 1163 Size: 573 462 21 4813 Fragments arranged by size: 4813 573 462 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 2351 3319 4080 5108 1540 Size: 275 186 96 254 968 761 1028 2301 Fragments arranged by size: 2301 1028 968 761 275 254 186 96 BsiHKAI G_wGCw'C Cuts at: 2229 2414 2931 3930 4015 5086 2229 Size: 185 517 999 85 1071 3012 Fragments arranged by size: 3012 1071 999 517 185 85 BsiWI C'GTAC_G Cuts at: 2283 2598 2283 Size: 315 5554 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 BsmBI CGTCTCn'nnnn_ Cuts at: 2812 2812 Size: 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2126 2229 2414 2724 2780 2931 Size: 399 264 72 103 185 310 56 151 Cuts at: 2931 3188 3930 4015 5086 1391 Size: 257 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 264 257 185 151 103 85 72 56 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 2721 3224 844 Size: 1376 501 503 3489 Fragments arranged by size: 3489 1376 503 501 BsrBI CCG'CTC Cuts at: 1394 1823 2302 3756 1394 Size: 429 479 1454 3507 Fragments arranged by size: 3507 1454 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2998 4364 4538 65 Size: 1183 1750 1366 174 1396 Fragments arranged by size: 1750 1396 1366 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2346 2548 4513 4700 1700 Size: 110 282 254 202 1965 187 2869 Fragments arranged by size: 2869 1965 282 254 202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 2540 3929 4941 2540 Size: 1389 1012 3468 Fragments arranged by size: 3468 1389 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 794 Size: 1526 4343 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstYI r'GATC_y Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864 Size: 1227 367 385 17 1452 86 12 346 Cuts at: 5864 1972 Size: 1977 Fragments arranged by size: 1977 1452 1227 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3048 3135 513 Size: 552 939 1044 87 3247 Fragments arranged by size: 3247 1044 939 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5130 Fragments arranged by size: 5130 683 56 BtsI GCAGTG_nn' Cuts at: 2202 2508 2587 3385 4179 4199 2202 Size: 306 79 798 794 20 3872 Fragments arranged by size: 3872 798 794 306 79 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2348 2689 Size: 54 1425 50 275 18 264 254 341 Cuts at: 2689 2791 2862 3022 3138 3316 4205 5834 8 Size: 102 71 160 116 178 889 1629 43 Fragments arranged by size: 1629 1425 889 341 275 264 254 178 160 116 102 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2094 2348 3316 1537 Size: 275 282 254 968 4090 Fragments arranged by size: 4090 968 282 275 254 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2441 3799 1353 Size: 917 171 1358 3423 Fragments arranged by size: 3423 1358 917 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2312 3025 4456 4830 4976 387 Size: 1064 138 723 713 1431 374 146 1280 Fragments arranged by size: 1431 1280 1064 723 713 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 2412 2227 Size: 185 5684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 3932 4529 5316 5584 1239 Size: 393 1031 1269 597 787 268 1524 Fragments arranged by size: 1524 1269 1031 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3095 3095 Size: 5869 EcoRI G'AATT_C Cuts at: 2996 3178 3556 2996 Size: 182 378 5309 Fragments arranged by size: 5309 378 182 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 2261 2471 5016 1678 Size: 583 210 2545 2531 Fragments arranged by size: 2545 2531 583 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2108 2140 4510 Size: 32 135 32 497 32 7 32 2370 Cuts at: 4510 4542 4584 4616 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2370 497 135 42 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 3213 3459 669 Size: 988 281 1275 246 3079 Fragments arranged by size: 3079 1275 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 2910 4983 5167 1305 Size: 750 855 2073 184 2007 Fragments arranged by size: 2073 2007 855 750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2691 2793 3024 10 Size: 54 1425 343 859 102 231 2855 Fragments arranged by size: 2855 1425 859 343 231 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 3827 4186 4345 518 Size: 648 2661 359 159 2042 Fragments arranged by size: 2661 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3285 3962 5110 5355 1606 Size: 1679 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 677 245 NaeI GCC'GGC Cuts at: 2094 2550 2094 Size: 456 5413 NcoI C'CATG_G Cuts at: 513 1065 2004 3048 513 Size: 552 939 1044 3334 Fragments arranged by size: 3334 1044 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NgoMIV G'CCGG_C Cuts at: 2092 2548 2092 Size: 456 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 2323 3068 3227 4772 1132 Size: 97 1094 745 159 1545 2229 Fragments arranged by size: 2229 1545 1094 745 159 97 PasI CC'CwG_GG Cuts at: 1348 2773 1348 Size: 1425 4444 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2773 1108 Size: 1665 4204 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3095 3095 Size: 5869 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PstI C_TGCA'G Cuts at: 830 2316 3221 830 Size: 1486 905 3478 Fragments arranged by size: 3478 1486 905 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 SacI G_AGCT'C Cuts at: 2229 2414 2229 Size: 185 5684 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SanDI GG'GwC_CC Cuts at: 3095 3095 Size: 5869 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 2498 4117 5829 2498 Size: 1619 1712 2538 Fragments arranged by size: 2538 1712 1619 SfcI C'TryA_G Cuts at: 826 1045 2312 2761 3157 3217 4352 5033 5224 Size: 219 1267 449 396 60 1135 681 191 Cuts at: 5224 826 Size: 1471 Fragments arranged by size: 1471 1267 1135 681 449 396 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 2112 2397 2423 3196 1872 Size: 240 285 26 773 4545 Fragments arranged by size: 4545 773 285 240 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3973 4874 5136 820 Size: 197 926 102 1928 901 262 1553 Fragments arranged by size: 1928 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 2004 2030 2172 2788 3048 3308 513 Size: 552 939 26 142 616 260 260 3074 Fragments arranged by size: 3074 939 616 552 260 260 142 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2889 3042 3896 3913 4066 4251 4696 Size: 1255 1309 153 854 17 153 185 445 Cuts at: 4696 325 Size: 1498 Fragments arranged by size: 1498 1309 1255 854 445 185 153 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4115 5827 Size: 274 80 33 51 1443 519 1619 1712 Cuts at: 5827 96 Size: 138 Fragments arranged by size: 1712 1619 1443 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3017 3049 5829 5861 3017 Size: 32 2780 32 3025 Fragments arranged by size: 3025 2780 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2266 1316 Size: 950 4919 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2684 3046 2684 Size: 362 5507 XhoI C'TCGA_G Cuts at: 1943 2045 1943 Size: 102 5767 XmaI C'CCGG_G Cuts at: 1870 2110 2395 2421 3194 1870 Size: 240 285 26 773 4545 Fragments arranged by size: 4545 773 285 240 26 XmnI GAAnn'nnTTC Cuts at: 2156 3998 2156 Size: 1842 4027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI