(Circular) (Six-base) MAPSORT of: pFN21AB8982.seq Check: 9156 from: 1 to: 5839 pFN21AB8982 5839 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3902 5286 5554 1632 Size: 2270 1384 268 1917 Fragments arranged by size: 2270 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 3000 1166 Size: 1834 4005 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 3582 5154 3582 Size: 1572 4267 ApaI G_GGCC'C Cuts at: 2208 2208 Size: 5839 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2635 3148 3374 3526 5635 1493 Size: 1142 513 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 513 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2575 3164 1870 Size: 73 138 494 589 4545 Fragments arranged by size: 4545 589 494 138 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2419 2554 2780 2825 Size: 325 217 627 239 393 135 226 45 Cuts at: 2825 3128 3160 4615 618 Size: 303 32 1455 1842 Fragments arranged by size: 1842 1455 627 393 325 303 239 226 217 135 45 32 BanII G_rGCy'C Cuts at: 1391 2012 2079 2208 2885 2921 2928 3158 1391 Size: 621 67 129 677 36 7 230 4072 Fragments arranged by size: 4072 677 621 230 129 67 36 7 BbeI G_GCGC'C Cuts at: 2030 2829 2030 Size: 799 5040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2708 928 Size: 971 809 4059 Fragments arranged by size: 4059 971 809 BbvCI CC'TCA_GC Cuts at: 2136 2987 2136 Size: 851 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3007 3041 3118 3152 4030 4064 1903 Size: 34 1070 34 77 34 878 34 3678 Fragments arranged by size: 3678 1070 878 77 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 1548 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2690 4450 136 Size: 107 122 71 631 605 1018 1760 1525 Fragments arranged by size: 1760 1525 1018 631 605 122 107 71 BglII A'GATC_T Cuts at: 2448 5834 2448 Size: 3386 2453 BlpI GC'TnA_GC Cuts at: 3041 3062 3256 3041 Size: 21 194 5624 Fragments arranged by size: 5624 194 21 Bme1580I G_kGCm'C Cuts at: 1790 2130 2208 2422 2559 2783 3131 3900 5056 Size: 340 78 214 137 224 348 769 1156 Cuts at: 5056 1790 Size: 2573 Fragments arranged by size: 2573 1156 769 348 340 224 214 137 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2047 2922 3022 Size: 416 53 83 399 24 630 875 100 Cuts at: 3022 3570 4528 442 Size: 548 958 1753 Fragments arranged by size: 1753 958 875 630 548 416 399 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4499 1239 Size: 3260 2579 Bpu10I CC'TnA_GC Cuts at: 2136 2922 2987 2136 Size: 786 65 4988 Fragments arranged by size: 4988 786 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3964 4829 5127 3964 Size: 865 298 4676 Fragments arranged by size: 4676 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2461 3630 493 Size: 711 1257 1169 2702 Fragments arranged by size: 2702 1257 1169 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2826 3646 4028 Size: 53 83 186 936 494 799 820 382 Cuts at: 4028 4663 275 Size: 635 1451 Fragments arranged by size: 1451 936 820 799 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2954 2984 3042 3072 1178 Size: 30 165 30 1551 30 58 30 3945 Fragments arranged by size: 3945 1551 165 58 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 1186 Size: 1180 4659 BseYI C'CCAG_C Cuts at: 2060 2129 2294 5042 5538 2060 Size: 69 165 2748 496 2361 Fragments arranged by size: 2748 2361 496 165 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 1163 Size: 573 561 4705 Fragments arranged by size: 4705 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 3063 3900 3985 5056 2012 Size: 1051 837 85 1071 2795 Fragments arranged by size: 2795 1071 1051 837 85 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2079 2130 2208 2422 2559 2783 Size: 399 222 67 51 78 214 137 224 Cuts at: 2783 2885 2921 2928 3063 3131 3158 3900 3985 Size: 102 36 7 135 68 27 742 85 Cuts at: 3985 5056 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 224 222 214 137 135 102 85 78 68 67 51 36 27 7 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2238 3194 844 Size: 1184 210 956 3489 Fragments arranged by size: 3489 1184 956 210 BsrBI CCG'CTC Cuts at: 1394 1823 2874 2977 3726 1394 Size: 429 1051 103 749 3507 Fragments arranged by size: 3507 1051 749 429 103 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 2498 4483 4670 1700 Size: 110 213 475 1985 187 2869 Fragments arranged by size: 2869 1985 475 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 2887 3899 4911 2887 Size: 1012 1012 3815 Fragments arranged by size: 3815 1012 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5839 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstXI CCAn_nnnn'nTGG Cuts at: 2270 3002 2270 Size: 732 5107 BstYI r'GATC_y Cuts at: 1972 2448 3053 3169 3536 3921 3938 5390 5476 Size: 476 605 116 367 385 17 1452 86 Cuts at: 5476 5488 5834 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 605 476 385 367 346 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2748 2748 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2403 2413 2739 513 Size: 552 1338 10 326 3613 Fragments arranged by size: 3613 1338 552 326 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2383 487 Size: 683 56 1157 3943 Fragments arranged by size: 3943 1157 683 56 BtsI GCAGTG_nn' Cuts at: 3355 4149 4169 3355 Size: 794 20 5025 Fragments arranged by size: 5025 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3085 3120 529 Size: 35 2521 35 3248 Fragments arranged by size: 3248 2521 35 35 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2372 2406 Size: 54 1425 50 275 18 238 304 34 Cuts at: 2406 2463 3286 4175 5804 8 Size: 57 823 889 1629 43 Fragments arranged by size: 1629 1425 889 823 304 275 238 57 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2648 3769 1353 Size: 1295 1121 3423 Fragments arranged by size: 3423 1295 1121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2507 4426 4800 4946 387 Size: 1064 138 918 1919 374 146 1280 Fragments arranged by size: 1919 1280 1064 918 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3902 4499 5286 5554 1239 Size: 393 2270 597 787 268 1524 Fragments arranged by size: 2270 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2261 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 2030 2434 2829 4986 1678 Size: 352 404 395 2157 2531 Fragments arranged by size: 2531 2157 404 395 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2657 2689 2753 2785 3045 Size: 32 135 32 1085 32 64 32 260 Cuts at: 3045 3077 4480 4512 4554 4586 1373 Size: 32 1403 32 42 32 2626 Fragments arranged by size: 2626 1403 1085 260 135 64 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3097 3183 3429 669 Size: 988 281 1159 86 246 3079 Fragments arranged by size: 3079 1159 988 281 246 86 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KasI G'GCGC_C Cuts at: 2026 2825 2026 Size: 799 5040 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 10 Size: 54 1425 343 576 3441 Fragments arranged by size: 3441 1425 576 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 3000 3035 3797 4156 4315 518 Size: 648 1236 598 35 762 359 159 2042 Fragments arranged by size: 2042 1236 762 648 598 359 159 35 MspA1I CmG'CkG Cuts at: 1606 2140 2287 3000 3255 3932 5080 5325 1606 Size: 534 147 713 255 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 534 255 245 147 NaeI GCC'GGC Cuts at: 2025 2500 2025 Size: 475 5364 NarI GG'CG_CC Cuts at: 2027 2826 2027 Size: 799 5040 NcoI C'CATG_G Cuts at: 513 1065 2403 513 Size: 552 1338 3949 Fragments arranged by size: 3949 1338 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NgoMIV G'CCGG_C Cuts at: 2023 2498 2023 Size: 475 5364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 3040 3197 4742 1132 Size: 97 1811 157 1545 2229 Fragments arranged by size: 2229 1811 1545 157 97 PasI CC'CwG_GG Cuts at: 1348 3120 1348 Size: 1772 4067 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2461 2899 1108 Size: 1353 438 4048 Fragments arranged by size: 4048 1353 438 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2606 2638 3908 3940 5421 5453 2606 Size: 32 1270 32 1481 32 2992 Fragments arranged by size: 2992 1481 1270 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PspOMI G'GGCC_C Cuts at: 2204 2204 Size: 5839 PstI C_TGCA'G Cuts at: 830 2048 3191 830 Size: 1218 1143 3478 Fragments arranged by size: 3478 1218 1143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2044 3187 4322 5003 5194 826 Size: 219 999 1143 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 999 681 219 191 SfoI GGC'GCC Cuts at: 2028 2827 2028 Size: 799 5040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 2577 3166 1872 Size: 705 589 4545 Fragments arranged by size: 4545 705 589 SmlI C'TyrA_G Cuts at: 820 1017 1943 3943 4844 5106 820 Size: 197 926 2000 901 262 1553 Fragments arranged by size: 2000 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 2267 1517 Size: 750 5089 StyI C'CwwG_G Cuts at: 513 1065 2403 2688 2898 3278 513 Size: 552 1338 285 210 380 3074 Fragments arranged by size: 3074 1338 552 380 285 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 3866 3883 4036 4221 4666 325 Size: 1255 550 1736 17 153 185 445 1498 Fragments arranged by size: 1736 1498 1255 550 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4085 5797 96 Size: 274 80 33 51 1443 2108 1712 138 Fragments arranged by size: 2108 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3042 3074 5799 5831 3042 Size: 32 2725 32 3050 Fragments arranged by size: 3050 2725 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 2575 3164 1870 Size: 705 589 4545 Fragments arranged by size: 4545 705 589 XmnI GAAnn'nnTTC Cuts at: 2108 2621 3968 2108 Size: 513 1347 3979 Fragments arranged by size: 3979 1347 513 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI