(Circular) (Six-base) MAPSORT of: pFN21AB8974.seq Check: 4595 from: 1 to: 5812 pFN21AB8974 5812 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 3155 1937 Size: 283 935 4594 Fragments arranged by size: 4594 935 283 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2654 2675 3020 3875 5259 5527 1632 Size: 1022 21 345 855 1384 268 1917 Fragments arranged by size: 1917 1384 1022 855 345 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2256 4711 5688 1203 Size: 1053 2455 977 1327 Fragments arranged by size: 2455 1327 1053 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 2237 1166 Size: 1071 4741 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 2269 3121 3347 3499 5608 1493 Size: 776 852 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 776 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 3133 4588 618 Size: 325 217 627 402 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 402 325 217 BanII G_rGCy'C Cuts at: 1391 3131 1391 Size: 1740 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2249 2736 928 Size: 971 189 161 487 4004 Fragments arranged by size: 4004 971 487 189 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 2412 1548 Size: 864 4948 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 3873 5029 1790 Size: 2083 1156 2573 Fragments arranged by size: 2573 2083 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3022 3543 4501 Size: 416 53 83 399 24 1605 521 958 Cuts at: 4501 442 Size: 1753 Fragments arranged by size: 1753 1605 958 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3104 4472 1239 Size: 1865 1368 2579 Fragments arranged by size: 2579 1865 1368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2844 3619 4001 4636 Size: 53 83 186 936 1311 775 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1451 1311 936 775 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 1186 Size: 1093 4719 BseYI C'CCAG_C Cuts at: 5015 5511 5015 Size: 496 5316 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 2581 2852 1163 Size: 573 479 366 271 4123 Fragments arranged by size: 4123 573 479 366 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2467 2627 3103 3873 3958 5029 2467 Size: 160 476 770 85 1071 3250 Fragments arranged by size: 3250 1071 770 476 160 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2467 2627 3103 3131 3873 3958 5029 Size: 399 677 160 476 28 742 85 1071 Cuts at: 5029 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 677 476 399 160 85 28 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3167 844 Size: 2323 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 2439 2445 2586 4307 4481 65 Size: 1183 1071 120 6 141 1721 174 1396 Fragments arranged by size: 1721 1396 1183 1071 174 141 120 6 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 2379 2493 96 Size: 2283 114 3415 Fragments arranged by size: 3415 2283 114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 2174 2987 3872 4884 2174 Size: 813 885 1012 3102 Fragments arranged by size: 3102 1012 885 813 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5812 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 2905 1184 Size: 1721 4091 BstYI r'GATC_y Cuts at: 1972 2983 3142 3509 3894 3911 5363 5449 5461 Size: 1011 159 367 385 17 1452 86 12 Cuts at: 5461 5807 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3094 3094 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 2076 2601 513 Size: 552 1011 525 3724 Fragments arranged by size: 3724 1011 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2631 2902 487 Size: 683 56 1405 271 3397 Fragments arranged by size: 3397 1405 683 271 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2869 2904 529 Size: 35 2305 35 3437 Fragments arranged by size: 3437 2305 35 35 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777 Size: 54 1425 50 275 18 1429 889 1629 Cuts at: 5777 8 Size: 43 Fragments arranged by size: 1629 1429 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 3742 1353 Size: 957 1432 3423 Fragments arranged by size: 3423 1432 957 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2966 4399 4773 4919 387 Size: 1064 138 1377 1433 374 146 1280 Fragments arranged by size: 1433 1377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 2675 3020 3104 3875 4472 5259 Size: 393 1022 21 345 84 771 597 787 Cuts at: 5259 5527 1239 Size: 268 1524 Fragments arranged by size: 1524 1022 787 771 597 393 345 268 84 21 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 5812 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 4453 4485 4527 Size: 32 135 32 470 32 2379 32 42 Cuts at: 4527 4559 1373 Size: 32 2626 Fragments arranged by size: 2626 2379 470 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2371 3156 3402 669 Size: 988 281 154 279 785 246 3079 Fragments arranged by size: 3079 988 785 281 279 246 154 HindIII A'AGCT_T Cuts at: 748 3058 748 Size: 2310 3502 HpaI GTT'AAC Cuts at: 2092 3402 2092 Size: 1310 4502 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 2277 3411 2277 Size: 1134 4678 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 4926 5110 1305 Size: 1584 2037 184 2007 Fragments arranged by size: 2037 2007 1584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2426 3770 4129 4288 518 Size: 648 1071 189 1344 359 159 2042 Fragments arranged by size: 2042 1344 1071 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2400 2534 2642 3228 3905 5053 5298 1606 Size: 794 134 108 586 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 586 245 134 108 NcoI C'CATG_G Cuts at: 513 1065 2076 2601 513 Size: 552 1011 525 3724 Fragments arranged by size: 3724 1011 552 525 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2260 2380 3170 4715 1132 Size: 97 1031 120 790 1545 2229 Fragments arranged by size: 2229 1545 1031 790 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 2256 4711 2256 Size: 2455 3357 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PstI C_TGCA'G Cuts at: 830 3164 830 Size: 2334 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2400 2534 2642 2400 Size: 134 108 5570 Fragments arranged by size: 5570 134 108 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2395 3160 4295 4976 5167 826 Size: 219 1350 765 1135 681 191 1471 Fragments arranged by size: 1471 1350 1135 765 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2076 2193 2601 3251 513 Size: 552 1011 117 408 650 3074 Fragments arranged by size: 3074 1011 650 552 408 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2177 2267 2924 2954 3839 3856 Size: 1255 446 151 90 657 30 885 17 Cuts at: 3856 4009 4194 4639 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 885 657 446 445 185 153 151 90 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2379 2493 Size: 274 80 33 51 1443 189 213 114 Cuts at: 2493 4058 5770 96 Size: 1565 1712 138 Fragments arranged by size: 1712 1565 1443 274 213 189 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2166 2524 2074 Size: 92 358 5362 Fragments arranged by size: 5362 358 92 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI