(Circular) (Six-base) MAPSORT of: pFN21AB8970.seq Check: 2686 from: 1 to: 5782 pFN21AB8970 5782 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2925 3845 5229 5497 1632 Size: 436 857 920 1384 268 1917 Fragments arranged by size: 1917 1384 920 857 436 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2042 2389 1166 Size: 876 347 4559 Fragments arranged by size: 4559 876 347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2407 1184 Size: 1223 4559 AlwNI CAG_nnn'CTG Cuts at: 2730 2776 3525 5097 2730 Size: 46 749 1572 3415 Fragments arranged by size: 3415 1572 749 46 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2134 3107 1870 Size: 73 191 973 4545 Fragments arranged by size: 4545 973 191 73 AvrII C'CTAG_G Cuts at: 2404 2404 Size: 5782 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2409 2543 2663 2706 3103 Size: 325 217 627 622 134 120 43 397 Cuts at: 3103 4558 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 622 397 325 217 134 120 43 BanII G_rGCy'C Cuts at: 1391 2355 2582 3101 1391 Size: 964 227 519 4072 Fragments arranged by size: 4072 964 519 227 BbeI G_GCGC'C Cuts at: 2667 2667 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2176 2922 928 Size: 971 277 746 3788 Fragments arranged by size: 3788 971 746 277 BbvCI CC'TCA_GC Cuts at: 2769 2769 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 4393 136 Size: 107 122 71 631 605 428 2293 1525 Fragments arranged by size: 2293 1525 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 2984 5777 2984 Size: 2793 2989 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2412 3843 4999 1790 Size: 622 1431 1156 2573 Fragments arranged by size: 2573 1431 1156 622 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2599 2798 2973 4442 1239 Size: 1169 191 199 175 1469 2579 Fragments arranged by size: 2579 1469 1169 199 191 175 Bpu10I CC'TnA_GC Cuts at: 2240 2769 2240 Size: 529 5253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 2938 2972 5776 2938 Size: 34 2804 2944 Fragments arranged by size: 2944 2804 34 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2158 2664 3589 3971 Size: 53 83 186 936 625 506 925 382 Cuts at: 3971 4606 275 Size: 635 1451 Fragments arranged by size: 1451 936 925 635 625 506 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2274 4215 4613 4887 5034 5445 2274 Size: 1941 398 274 147 411 2611 Fragments arranged by size: 2611 1941 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2355 2385 2699 2729 1178 Size: 30 165 30 952 30 314 30 4231 Fragments arranged by size: 4231 952 314 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2347 2588 2795 1186 Size: 1161 241 207 4173 Fragments arranged by size: 4173 1161 241 207 BseYI C'CCAG_C Cuts at: 2140 2224 2593 4985 5481 2140 Size: 84 369 2392 496 2441 Fragments arranged by size: 2441 2392 496 369 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2549 2704 3070 1163 Size: 573 813 155 366 3875 Fragments arranged by size: 3875 813 573 366 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 2153 2610 2823 3843 3928 4999 2082 Size: 71 457 213 1020 85 1071 2865 Fragments arranged by size: 2865 1071 1020 457 213 85 71 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2153 2355 2412 2582 2610 2823 Size: 399 292 71 202 57 170 28 213 Cuts at: 2823 3101 3843 3928 4999 1391 Size: 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 292 278 213 202 170 85 71 57 28 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 3137 844 Size: 1271 1022 3489 Fragments arranged by size: 3489 1271 1022 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 2691 3045 4426 4613 1700 Size: 110 280 601 354 1381 187 2869 Fragments arranged by size: 2869 1381 601 354 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 2129 2496 2743 1925 Size: 204 367 247 4964 Fragments arranged by size: 4964 367 247 204 BssSI C'ACGA_G Cuts at: 2570 3842 4854 2570 Size: 1272 1012 3498 Fragments arranged by size: 3498 1272 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2550 794 Size: 1756 4026 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 2212 1184 Size: 1028 4754 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2386 2623 3067 2044 Size: 342 237 444 4759 Fragments arranged by size: 4759 444 342 237 BstYI r'GATC_y Cuts at: 1972 2984 3112 3479 3864 3881 5333 5419 5431 Size: 1012 128 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1012 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2379 513 Size: 552 1314 3916 Fragments arranged by size: 3916 1314 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2699 487 Size: 683 56 1473 3570 Fragments arranged by size: 3570 1473 683 56 BtsI GCAGTG_nn' Cuts at: 2162 2363 3298 4092 4112 2162 Size: 201 935 794 20 3832 Fragments arranged by size: 3832 935 794 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DraIII CAC_nnn'GTG Cuts at: 2550 2832 2550 Size: 282 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2376 3229 4118 Size: 54 1425 50 275 18 546 853 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 853 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2828 3712 1353 Size: 734 741 884 3423 Fragments arranged by size: 3423 884 741 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2408 2599 2798 2925 2973 3845 Size: 393 436 340 191 199 127 48 872 Cuts at: 3845 4442 5229 5497 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 872 787 597 436 393 340 268 199 191 127 48 EcoNI CCTnn'n_nnAGG Cuts at: 2722 2722 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 2898 2898 Size: 5782 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 2672 4288 2672 Size: 1616 4166 HaeII r_GCGC'y Cuts at: 1678 2478 2630 2667 2810 4929 1678 Size: 800 152 37 143 2119 2531 Fragments arranged by size: 2531 2119 800 152 143 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 4423 4455 4497 Size: 32 135 32 846 32 1973 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1973 846 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2663 2663 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 2952 3381 2952 Size: 429 5353 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3414 Fragments arranged by size: 3414 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2195 2389 3065 3740 4099 4258 Size: 648 876 153 194 676 675 359 159 Cuts at: 4258 518 Size: 2042 Fragments arranged by size: 2042 876 676 675 648 359 194 159 153 MspA1I CmG'CkG Cuts at: 1606 2025 2173 2704 3198 3875 5023 5268 1606 Size: 419 148 531 494 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 494 419 245 148 NarI GG'CG_CC Cuts at: 2664 2664 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2379 513 Size: 552 1314 3916 Fragments arranged by size: 3916 1314 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2194 3140 4685 1132 Size: 97 965 946 1545 2229 Fragments arranged by size: 2229 1545 965 946 97 PasI CC'CwG_GG Cuts at: 1348 2116 1348 Size: 768 5014 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2024 2056 3851 3883 5364 5396 2024 Size: 32 1795 32 1481 32 2410 Fragments arranged by size: 2410 1795 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2898 2898 Size: 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2703 3134 830 Size: 1873 431 3478 Fragments arranged by size: 3478 1873 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2087 2828 2087 Size: 741 5041 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 2534 4030 5742 2534 Size: 1496 1712 2574 Fragments arranged by size: 2574 1712 1496 SexAI A'CCwGG_T Cuts at: 2546 2546 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 2699 3130 4265 4946 5137 826 Size: 219 1654 431 1135 681 191 1471 Fragments arranged by size: 1654 1471 1135 681 431 219 191 SfoI GGC'GCC Cuts at: 2665 2665 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 2691 1700 Size: 991 4791 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2379 2404 2963 3221 513 Size: 552 1314 25 559 258 3074 Fragments arranged by size: 3074 1314 559 552 258 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 3809 3826 3979 4164 4609 325 Size: 1255 590 1639 17 153 185 445 1498 Fragments arranged by size: 1639 1498 1255 590 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 4028 5740 Size: 274 80 33 51 1443 555 1496 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1496 1443 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2355 2387 2699 2731 5742 5774 2355 Size: 32 312 32 3011 32 2363 Fragments arranged by size: 3011 2363 312 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI