Restriction map for
pFN21AB8959
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AlwNI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BbrPI BbsI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsiEI BsiWI BsmFI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I Eco81I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MaeI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
TspRI Tth111I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 402 TCCGGA T^CCGGA
AcsI 58 AAATTC [GA]^AATT[CT]
AhdI 535 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 331 GTGCAC G^TGCAC
Aor51HI 575 AGCGCT AGC^GCT
ApaLI 331 GTGCAC G^TGCAC
ApoI 58 AAATTC [GA]^AATT[CT]
AspEI 535 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 865 CTCGAG C^[CT]CG[GA]G
AvaII 933 GGACC G^G[AT]CC
BalI 5 TGGCCA TGG^CCA
BbvCI 528 CCTCAGC CCTCAGC(-5/-2)
BcgI 833 GCACGTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 341 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 666 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 666 GCTGAGC GC^T[ACGT]AGC
BsaWI 402 TCCGGA [AT]^CCGG[AT]
BseAI 402 TCCGGA T^CCGGA
BseRI 757 GAGGAG GAGGAG(10/8)
BsmBI 195 CGTCTC CGTCTC(1/5)
BsoBI 865 CTCGAG C^[CT]CG[GA]G
BspEI 402 TCCGGA T^CCGGA
BsrBI 387 GAGCGG CCGCTC(-3/-3)
BsrFI 735 GCCGGT [GA]^CCGG[CT]
BstEII 606 GGTGACC G^GT[ACGT]ACC
BstPI 606 GGTGACC G^GT[ACGT]ACC
CelII 666 GCTGAGC GC^T[ACGT]AGC
Cfr10I 735 GCCGGT [GA]^CCGG[CT]
DraII 338 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 535 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 802 CTCTTC CTCTTC(1/4)
Eco47III 575 AGCGCT AGC^GCT
EcoNI 462 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 338 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 606 GGTGACC G^GT[ACGT]ACC
EcoRV 190 GATATC GAT^ATC
HgaI 229 GACGC GACGC(5/10)
HindIII 141 AAGCTT A^AGCTT
Ksp632I 802 CTCTTC CTCTTC(1/4)
MluNI 5 TGGCCA TGG^CCA
MroI 402 TCCGGA T^CCGGA
MscI 5 TGGCCA TGG^CCA
MseI 938 TTAA T^TAA
MslI 474 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 865 CTCGAG C^TCGAG
PflMI 468 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 799 GAGCTC GAGCT^C
SapI 801 GCTCTTC GCTCTTC(1/4)
Tru9I 938 TTAA T^TAA
Tsp509I 59 AATT ^AATT
Van91I 468 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 865 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 429 GATCC GGATC(4/5)
648 GATCC
BanI 448 GGCGCC G^G[CT][GA]CC
846 GGCGCC
BanII 660 GGGCTC G[GA]GC[CT]^C
799 GAGCTC
BbeI 448 GGCGCC GGCGC^C
846 GGCGCC
BcnI 39 CCCGG CC^[GC]GG
652 CCCGG
BsrI 930 ACTGG ACTGG(1/-1)
65 CCAGT
BstAPI 36 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
677 GCACCATCTGC
BstF5I 214 CATCC GGATG(2/0)
433 CATCC
BstYI 428 AGATCC [GA]^GATC[CT]
647 AGATCC
EaeI 5 TGGCCA [CT]^GGCC[GA]
914 CGGCCA
EcoT14I 452 CCATGG C^C[AT][AT]GG
473 CCATGG
FokI 214 CATCC GGATG(9/13)
433 CATCC
KasI 448 GGCGCC G^GCGCC
846 GGCGCC
MaeIII 485 GTCAC ^GT[ACGT]AC
607 GTGAC
MflI 428 AGATCC [GA]^GATC[CT]
647 AGATCC
NarI 448 GGCGCC GG^CGCC
846 GGCGCC
NciI 39 CCCGG CC^[GC]GG
652 CCCGG
NcoI 452 CCATGG C^CATGG
473 CCATGG
NspI 32 GCATGC [GA]CATG^[CT]
523 GCATGC
PstI 121 CTGCAG CTGCA^G
684 CTGCAG
PvuII 118 CAGCTG CAG^CTG
299 CAGCTG
SfaNI 417 GATGC GCATC(5/9)
593 GCATC
SfcI 121 CTGCAG C^T[GA][CT]AG
684 CTGCAG
SfoI 448 GGCGCC GGC^GCC
846 GGCGCC
SphI 32 GCATGC GCATG^C
523 GCATGC
StuI 746 AGGCCT AGG^CCT
869 AGGCCT
StyI 452 CCATGG C^C[AT][AT]GG
473 CCATGG
Tsp45I 485 GTCAC ^GT[GC]AC
607 GTGAC
XhoII 428 AGATCC [GA]^GATC[CT]
647 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BbvI BmyI BpmI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraIII DsaI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SmlI TaqI TseI TthHB8I