(Circular) (Six-base) MAPSORT of: pFN21AB8959.seq Check: 6039 from: 1 to: 5719 pFN21AB8959 5719 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 2475 Size: 5719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 5166 5434 1632 Size: 2150 1384 268 1917 Fragments arranged by size: 2150 1917 1384 268 AfeI AGC'GCT Cuts at: 2582 2582 Size: 5719 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 2545 4448 2545 Size: 1903 3816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaLI G'TGCA_C Cuts at: 2336 3776 4932 2336 Size: 1440 1156 3123 Fragments arranged by size: 3123 1440 1156 ApoI r'AATT_y Cuts at: 1493 2063 3028 3254 3406 5515 1493 Size: 570 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 570 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2870 3044 1870 Size: 73 927 174 4545 Fragments arranged by size: 4545 927 174 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 2851 3040 4495 618 Size: 325 217 627 666 398 189 1455 1842 Fragments arranged by size: 1842 1455 666 627 398 325 217 189 BanII G_rGCy'C Cuts at: 1391 2669 2808 3038 1391 Size: 1278 139 230 4072 Fragments arranged by size: 4072 1278 230 139 BbeI G_GCGC'C Cuts at: 2457 2855 2457 Size: 398 5321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4748 BbvCI CC'TCA_GC Cuts at: 2534 2534 Size: 5719 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2827 2861 3910 3944 1903 Size: 34 890 34 1049 34 3678 Fragments arranged by size: 3678 1049 890 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2352 4330 Size: 107 122 71 631 605 337 343 1978 Cuts at: 4330 136 Size: 1525 Fragments arranged by size: 1978 1525 631 605 343 337 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 2672 3136 2672 Size: 464 5255 Bme1580I G_kGCm'C Cuts at: 1790 2265 2292 2340 3780 4936 1790 Size: 475 27 48 1440 1156 2573 Fragments arranged by size: 2573 1440 1156 475 48 27 BmgBI CAC'GTC Cuts at: 1318 2271 2542 2626 2841 2897 1318 Size: 953 271 84 215 56 4140 Fragments arranged by size: 4140 953 271 215 84 56 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3450 4408 442 Size: 416 53 83 399 24 2033 958 1753 Fragments arranged by size: 2033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2126 2389 2126 Size: 263 5456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2384 2732 4379 1239 Size: 814 331 348 1647 2579 Fragments arranged by size: 2579 1647 814 348 331 Bpu10I CC'TnA_GC Cuts at: 2534 2534 Size: 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 2720 3844 4709 5007 2633 Size: 87 1124 865 298 3345 Fragments arranged by size: 3345 1124 865 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 2454 2852 3526 Size: 53 83 186 936 702 219 398 674 Cuts at: 3526 3908 4543 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 702 674 635 398 382 219 186 83 53 BsaWI w'CCGG_w Cuts at: 2407 4152 4550 4824 4971 5382 2407 Size: 1745 398 274 147 411 2744 Fragments arranged by size: 2744 1745 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 1178 Size: 30 165 30 666 30 4798 Fragments arranged by size: 4798 666 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 1186 Size: 1591 4128 BseYI C'CCAG_C Cuts at: 2113 2302 2605 4922 5418 2113 Size: 189 303 2317 496 2414 Fragments arranged by size: 2414 2317 496 303 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 2251 2453 1163 Size: 573 324 191 202 4429 Fragments arranged by size: 4429 573 324 202 191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2370 2748 2808 3780 3865 4936 2340 Size: 30 378 60 972 85 1071 3123 Fragments arranged by size: 3123 1071 972 378 85 60 30 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 BsmBI CGTCTCn'nnnn_ Cuts at: 2206 2206 Size: 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2292 2340 2370 2669 2748 2808 Size: 399 475 27 48 30 299 79 60 Cuts at: 2808 3038 3780 3865 4936 1391 Size: 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 299 230 85 79 60 48 30 27 BspEI T'CCGG_A Cuts at: 2407 5382 2407 Size: 2975 2744 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2367 2475 2682 3074 844 Size: 1523 108 207 392 3489 Fragments arranged by size: 3489 1523 392 207 108 BsrBI CCG'CTC Cuts at: 1394 1823 2394 3606 1394 Size: 429 571 1212 3507 Fragments arranged by size: 3507 1212 571 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2740 4363 4550 1700 Size: 110 930 1623 187 2869 Fragments arranged by size: 2869 1623 930 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 2688 794 Size: 1253 641 3825 Fragments arranged by size: 3825 1253 641 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 2611 1184 Size: 1427 4292 BstYI r'GATC_y Cuts at: 1972 2433 2652 3049 3416 3801 3818 5270 5356 Size: 461 219 397 367 385 17 1452 86 Cuts at: 5356 5368 5714 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 461 397 385 367 346 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2206 2457 2478 2502 513 Size: 552 1141 251 21 24 3730 Fragments arranged by size: 3730 1141 552 251 24 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2830 2908 487 Size: 683 56 1604 78 3298 Fragments arranged by size: 3298 1604 683 78 56 BtsI GCAGTG_nn' Cuts at: 3235 4029 4049 3235 Size: 794 20 4905 Fragments arranged by size: 4905 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DraIII CAC_nnn'GTG Cuts at: 2047 2335 2419 2503 2047 Size: 288 84 84 5263 Fragments arranged by size: 5263 288 84 84 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2919 3166 Size: 54 1425 50 275 18 180 909 247 Cuts at: 3166 4055 5684 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 909 889 275 247 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2813 3649 1353 Size: 1460 836 3423 Fragments arranged by size: 3423 1460 836 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 4306 4680 4826 387 Size: 1064 138 1047 1670 374 146 1280 Fragments arranged by size: 1670 1280 1064 1047 374 146 138 EcoICRI GAG'CTC Cuts at: 2806 2806 Size: 5719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2384 2732 3782 4379 5166 5434 Size: 393 421 331 348 1050 597 787 268 Cuts at: 5434 1239 Size: 1524 Fragments arranged by size: 1524 1050 787 597 421 393 348 331 268 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5719 EcoO109I rG'GnC_Cy Cuts at: 2344 2344 Size: 5719 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 EcoRV GAT'ATC Cuts at: 2197 2197 Size: 5719 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2406 2457 2520 2584 2855 4866 1678 Size: 728 51 63 64 271 2011 2531 Fragments arranged by size: 2531 2011 728 271 64 63 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2531 2563 4360 Size: 32 135 32 614 32 313 32 1797 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1797 614 313 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 2146 748 Size: 1398 4321 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KasI G'GCGC_C Cuts at: 2453 2851 2453 Size: 398 5321 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2739 4833 5017 1305 Size: 1434 2094 184 2007 Fragments arranged by size: 2094 2007 1434 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 10 Size: 54 1425 343 180 3717 Fragments arranged by size: 3717 1425 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 3677 4036 4195 518 Size: 648 1317 1194 359 159 2042 Fragments arranged by size: 2042 1317 1194 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 2306 2593 3135 3812 4960 5205 1606 Size: 519 181 287 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 542 519 287 245 181 NarI GG'CG_CC Cuts at: 2454 2852 2454 Size: 398 5321 NcoI C'CATG_G Cuts at: 513 1065 2457 2478 513 Size: 552 1392 21 3754 Fragments arranged by size: 3754 1392 552 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2041 2532 3077 4622 1132 Size: 97 812 491 545 1545 2229 Fragments arranged by size: 2229 1545 812 545 491 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4348 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspXI vC'TCGA_Gb Cuts at: 2870 2870 Size: 5719 PstI C_TGCA'G Cuts at: 830 2130 2693 3071 830 Size: 1300 563 378 3478 Fragments arranged by size: 3478 1300 563 378 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2125 2306 2125 Size: 181 5538 SacI G_AGCT'C Cuts at: 2808 2808 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2813 2813 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 2126 2689 3067 4202 4883 5074 826 Size: 219 1081 563 378 1135 681 191 1471 Fragments arranged by size: 1471 1135 1081 681 563 378 219 191 SfoI GGC'GCC Cuts at: 2455 2853 2455 Size: 398 5321 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 2735 2870 3823 4724 4986 Size: 197 926 705 87 135 953 901 262 Cuts at: 4986 820 Size: 1553 Fragments arranged by size: 1553 953 926 901 705 262 197 135 87 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 2041 2532 3077 1132 Size: 909 491 545 3774 Fragments arranged by size: 3774 909 545 491 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 2753 2876 1517 Size: 1236 123 4360 Fragments arranged by size: 4360 1236 123 StyI C'CwwG_G Cuts at: 513 1065 2457 2478 3158 513 Size: 552 1392 21 680 3074 Fragments arranged by size: 3074 1392 680 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 3746 3763 3916 4101 4546 325 Size: 1255 736 1430 17 153 185 445 1498 Fragments arranged by size: 1498 1430 1255 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3965 5677 96 Size: 274 80 33 51 1443 1988 1712 138 Fragments arranged by size: 1988 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 5679 5711 2069 Size: 32 3578 32 2077 Fragments arranged by size: 3578 2077 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 2870 1943 Size: 927 4792 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI