(Circular) (Six-base) MAPSORT of: pFN21AB8958.seq Check: 2792 from: 1 to: 5710 pFN21AB8958 5710 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 2370 3031 2370 Size: 661 5049 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 3773 5157 5425 1632 Size: 667 1474 1384 268 1917 Fragments arranged by size: 1917 1474 1384 667 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2337 4609 5586 1203 Size: 1134 2272 977 1327 Fragments arranged by size: 2272 1327 1134 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 1184 Size: 1011 4699 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 2729 Size: 32 5678 AlwNI CAG_nnn'CTG Cuts at: 2515 3453 5025 2515 Size: 938 1572 3200 Fragments arranged by size: 3200 1572 938 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2079 2971 3035 1870 Size: 73 119 17 892 64 4545 Fragments arranged by size: 4545 892 119 73 64 17 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2370 3031 4486 618 Size: 325 217 627 583 661 1455 1842 Fragments arranged by size: 1842 1455 661 627 583 325 217 BanII G_rGCy'C Cuts at: 1391 2471 3029 1391 Size: 1080 558 4072 Fragments arranged by size: 4072 1080 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 928 Size: 971 1071 3668 Fragments arranged by size: 3668 1071 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2826 5705 2826 Size: 2879 2831 BlpI GC'TnA_GC Cuts at: 2074 3127 2074 Size: 1053 4657 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 3441 4399 Size: 416 53 83 399 24 954 1070 958 Cuts at: 4399 442 Size: 1753 Fragments arranged by size: 1753 1070 958 954 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 2756 4370 1239 Size: 1350 167 1614 2579 Fragments arranged by size: 2579 1614 1350 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2266 3835 4700 4998 2266 Size: 1569 865 298 2978 Fragments arranged by size: 2978 1569 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 2882 4373 882 Size: 617 1308 75 1491 2219 Fragments arranged by size: 2219 1491 1308 617 75 BsaAI yAC'GTr Cuts at: 493 1204 2529 3501 493 Size: 711 1325 972 2702 Fragments arranged by size: 2702 1325 972 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2587 2617 2729 2759 1178 Size: 30 165 30 1184 30 112 30 4129 Fragments arranged by size: 4129 1184 165 112 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5710 BseYI C'CCAG_C Cuts at: 2229 2453 4913 5409 2229 Size: 224 2460 496 2530 Fragments arranged by size: 2530 2460 496 224 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 1163 Size: 573 465 4672 Fragments arranged by size: 4672 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 3160 3921 4949 1540 Size: 275 186 157 1002 761 1028 2301 Fragments arranged by size: 2301 1028 1002 761 275 186 157 BsiHKAI G_wGCw'C Cuts at: 2508 3771 3856 4927 2508 Size: 1263 85 1071 3291 Fragments arranged by size: 3291 1263 1071 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2471 2508 3029 3771 3856 4927 1391 Size: 399 681 37 521 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 521 399 85 37 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 3065 844 Size: 1234 987 3489 Fragments arranged by size: 3489 1234 987 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2233 4354 4541 1700 Size: 110 423 2121 187 2869 Fragments arranged by size: 2869 2121 423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2770 1925 Size: 845 4865 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2495 2495 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2826 3040 3407 3792 3809 5261 5347 5359 Size: 854 214 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 854 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3805 Fragments arranged by size: 3805 939 552 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 2059 3226 4020 4040 2059 Size: 1167 794 20 3729 Fragments arranged by size: 3729 1167 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2267 2302 529 Size: 35 1703 35 3937 Fragments arranged by size: 3937 1703 35 35 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2895 3157 4046 Size: 54 1425 50 275 18 1065 262 889 Cuts at: 4046 5675 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1065 889 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2956 3640 1353 Size: 1603 684 3423 Fragments arranged by size: 3423 1603 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2299 2589 2756 3773 4370 5157 5425 Size: 393 667 290 167 1017 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 1017 787 667 597 393 290 268 167 EcoO109I rG'GnC_Cy Cuts at: 2067 2067 Size: 5710 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 2702 4216 2702 Size: 1514 4196 FspAI rTGC'GCAy Cuts at: 2702 2702 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2136 4857 1678 Size: 458 2721 2531 Fragments arranged by size: 2721 2531 458 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2587 2619 4351 4383 4425 Size: 32 135 32 1015 32 1732 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 1732 1015 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2687 3054 3300 669 Size: 988 281 749 367 246 3079 Fragments arranged by size: 3079 988 749 367 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 2374 3035 2374 Size: 661 5049 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 2337 5586 2337 Size: 3249 2461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2644 4824 5008 1305 Size: 1339 2180 184 2007 Fragments arranged by size: 2180 2007 1339 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2493 3668 4027 4186 518 Size: 648 1327 1175 359 159 2042 Fragments arranged by size: 2042 1327 1175 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2390 3126 3803 4951 5196 1606 Size: 784 736 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 736 677 245 NaeI GCC'GGC Cuts at: 2235 2235 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3805 Fragments arranged by size: 3805 939 552 414 NdeI CA'TA_TG Cuts at: 387 2494 387 Size: 2107 3603 NgoMIV G'CCGG_C Cuts at: 2233 2233 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2300 2515 1108 Size: 1192 215 4303 Fragments arranged by size: 4303 1192 215 PfoI T'CCnGG_A Cuts at: 2031 2252 2031 Size: 221 5489 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2729 2761 3779 3811 5292 5324 2729 Size: 32 1018 32 1481 32 3115 Fragments arranged by size: 3115 1481 1018 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 2067 Size: 5710 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2805 3062 830 Size: 1975 257 3478 Fragments arranged by size: 3478 1975 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2956 2956 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2801 3058 4193 4874 5065 826 Size: 219 1756 257 1135 681 191 1471 Fragments arranged by size: 1756 1471 1135 681 257 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 2973 3037 1872 Size: 1101 64 4545 Fragments arranged by size: 4545 1101 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 2281 3814 4715 4977 820 Size: 197 926 338 1533 901 262 1553 Fragments arranged by size: 1553 1533 926 901 338 262 197 SnaBI TAC'GTA Cuts at: 493 2529 3501 493 Size: 2036 972 2702 Fragments arranged by size: 2702 2036 972 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2418 3149 513 Size: 552 939 414 731 3074 Fragments arranged by size: 3074 939 731 552 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3737 3754 3907 4092 4537 325 Size: 1255 2157 17 153 185 445 1498 Fragments arranged by size: 2157 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2285 2450 2285 Size: 165 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 2971 3035 1870 Size: 1101 64 4545 Fragments arranged by size: 4545 1101 64 XmnI GAAnn'nnTTC Cuts at: 2027 2744 3839 2027 Size: 717 1095 3898 Fragments arranged by size: 3898 1095 717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI