(Circular) (Six-base) MAPSORT of: pFN21AB8953.seq Check: 4974 from: 1 to: 5689 pFN21AB8953 5689 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2302 2302 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2593 3752 5136 5404 1632 Size: 961 1159 1384 268 1917 Fragments arranged by size: 1917 1384 1159 961 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2025 4588 5565 1203 Size: 822 2563 977 1327 Fragments arranged by size: 2563 1327 977 822 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 2389 4418 2389 Size: 2029 3660 AleI CACnn'nnGTG Cuts at: 1166 2529 1166 Size: 1363 4326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaLI G'TGCA_C Cuts at: 2257 3746 4902 2257 Size: 1489 1156 3044 Fragments arranged by size: 3044 1489 1156 ApoI r'AATT_y Cuts at: 1493 2707 2998 3224 3376 5485 1493 Size: 1214 291 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2356 2425 2769 3014 1870 Size: 73 413 69 344 245 4545 Fragments arranged by size: 4545 413 344 245 73 69 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2147 2285 2561 3010 Size: 325 217 627 287 73 138 276 449 Cuts at: 3010 4465 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 449 325 287 276 217 138 73 BanII G_rGCy'C Cuts at: 1391 2012 2776 3008 1391 Size: 621 764 232 4072 Fragments arranged by size: 4072 764 621 232 BbeI G_GCGC'C Cuts at: 2151 2151 Size: 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2413 928 Size: 971 514 4204 Fragments arranged by size: 4204 971 514 BbvCI CC'TCA_GC Cuts at: 2447 2447 Size: 5689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2267 2301 3880 3914 1903 Size: 34 330 34 1579 34 3678 Fragments arranged by size: 3678 1579 330 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2414 4300 136 Size: 107 122 71 631 605 742 1886 1525 Fragments arranged by size: 1886 1525 742 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2066 3106 2066 Size: 1040 4649 Bme1580I G_kGCm'C Cuts at: 1790 2261 3750 4906 1790 Size: 471 1489 1156 2573 Fragments arranged by size: 2573 1489 1156 471 BmgBI CAC'GTC Cuts at: 1318 2062 2398 1318 Size: 744 336 4609 Fragments arranged by size: 4609 744 336 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2239 3420 4378 Size: 416 53 83 399 24 822 1181 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1181 958 822 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2685 1056 Size: 1629 4060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2285 2674 4349 1239 Size: 800 246 389 1675 2579 Fragments arranged by size: 2579 1675 800 389 246 Bpu10I CC'TnA_GC Cuts at: 2447 2447 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2397 4352 882 Size: 617 898 1955 2219 Fragments arranged by size: 2219 1955 898 617 BsaAI yAC'GTr Cuts at: 493 1204 2026 3480 493 Size: 711 822 1454 2702 Fragments arranged by size: 2702 1454 822 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2148 3496 3878 Size: 53 83 186 936 564 51 1348 382 Cuts at: 3878 4513 275 Size: 635 1451 Fragments arranged by size: 1451 1348 936 635 564 382 186 83 53 51 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 1178 Size: 30 165 30 609 30 4825 Fragments arranged by size: 4825 609 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 1186 Size: 1249 4440 BseYI C'CCAG_C Cuts at: 2306 2365 4892 5388 2306 Size: 59 2527 496 2607 Fragments arranged by size: 2607 2527 496 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 2219 2238 2379 2418 3139 Size: 275 186 204 14 19 141 39 721 Cuts at: 3139 3900 4928 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 721 275 204 186 141 39 19 14 BsiHKAI G_wGCw'C Cuts at: 2012 2067 2261 3750 3835 4906 2012 Size: 55 194 1489 85 1071 2795 Fragments arranged by size: 2795 1489 1071 194 85 55 BsiWI C'GTAC_G Cuts at: 2346 2346 Size: 5689 BsmI GAATG_Cn' Cuts at: 2647 3200 3293 2647 Size: 553 93 5043 Fragments arranged by size: 5043 553 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2584 2584 Size: 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2067 2261 2776 3008 3750 3835 Size: 399 222 55 194 515 232 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 515 399 232 222 194 85 55 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2302 3044 844 Size: 1458 742 3489 Fragments arranged by size: 3489 1458 742 BsrBI CCG'CTC Cuts at: 1394 1823 2091 3576 1394 Size: 429 268 1485 3507 Fragments arranged by size: 3507 1485 429 268 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2341 4333 4520 1700 Size: 110 531 1992 187 2869 Fragments arranged by size: 2869 1992 531 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2899 2899 Size: 5689 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 2298 513 Size: 552 1041 192 3904 Fragments arranged by size: 3904 1041 552 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2261 3205 3999 4019 2261 Size: 944 794 20 3931 Fragments arranged by size: 3931 944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2819 4696 809 Size: 507 1503 1877 1802 Fragments arranged by size: 1877 1802 1503 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2202 2216 Size: 54 1425 50 275 18 342 30 14 Cuts at: 2216 2235 2376 2415 2452 2521 2883 3136 4025 Size: 19 141 39 37 69 362 253 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 362 342 275 253 141 69 54 50 43 39 37 30 19 18 14 EagI C'GGCC_G Cuts at: 1537 1812 2202 2216 2235 2376 2415 3136 1537 Size: 275 390 14 19 141 39 721 4090 Fragments arranged by size: 4090 721 390 275 141 39 19 14 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 3619 1353 Size: 1277 989 3423 Fragments arranged by size: 3423 1277 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2285 2593 2674 3752 4349 5136 Size: 393 407 246 308 81 1078 597 787 Cuts at: 5136 5404 1239 Size: 268 1524 Fragments arranged by size: 1524 1078 787 597 407 393 308 268 246 81 EcoO109I rG'GnC_Cy Cuts at: 2271 2271 Size: 5689 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2151 4836 1678 Size: 473 2685 2531 Fragments arranged by size: 2685 2531 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2375 2407 4330 Size: 32 135 32 440 32 331 32 1923 Cuts at: 4330 4362 4404 4436 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1923 440 331 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2147 2147 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2935 4803 4987 1305 Size: 813 817 1868 184 2007 Fragments arranged by size: 2007 1868 817 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2885 10 Size: 54 1425 343 1053 2814 Fragments arranged by size: 2814 1425 1053 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2529 2808 2897 3647 4006 4165 518 Size: 648 1363 279 89 750 359 159 2042 Fragments arranged by size: 2042 1363 750 648 359 279 159 89 MspA1I CmG'CkG Cuts at: 1606 2043 2160 2300 2306 2451 3105 3782 4930 Size: 437 117 140 6 145 654 677 1148 Cuts at: 4930 5175 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 654 437 245 145 140 117 6 NaeI GCC'GGC Cuts at: 2343 2343 Size: 5689 NarI GG'CG_CC Cuts at: 2148 2148 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2341 2341 Size: 5689 NheI G'CTAG_C Cuts at: 1052 2681 1052 Size: 1629 4060 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspXI vC'TCGA_Gb Cuts at: 2769 2769 Size: 5689 PstI C_TGCA'G Cuts at: 830 2118 3041 830 Size: 1288 923 3478 Fragments arranged by size: 3478 1288 923 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2160 2306 2160 Size: 146 5543 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5689 SacII CC_GC'GG Cuts at: 2301 2301 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2762 3937 5649 2762 Size: 1175 1712 2802 Fragments arranged by size: 2802 1712 1175 SexAI A'CCwGG_T Cuts at: 2281 2281 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2114 3037 4172 4853 5044 826 Size: 219 1069 923 1135 681 191 1471 Fragments arranged by size: 1471 1135 1069 923 681 219 191 SfoI GGC'GCC Cuts at: 2149 2149 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2358 3016 1872 Size: 486 658 4545 Fragments arranged by size: 4545 658 486 SmlI C'TyrA_G Cuts at: 820 1017 1943 2769 3793 4694 4956 820 Size: 197 926 826 1024 901 262 1553 Fragments arranged by size: 1553 1024 926 901 826 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 3128 513 Size: 552 2063 3074 Fragments arranged by size: 3074 2063 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 3716 3733 3886 4071 4516 325 Size: 1255 512 1624 17 153 185 445 1498 Fragments arranged by size: 1624 1498 1255 512 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2760 3935 5647 Size: 274 80 33 51 1443 783 1175 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1175 783 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2878 2910 5649 5681 2878 Size: 32 2739 32 2886 Fragments arranged by size: 2886 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 2769 1943 Size: 826 4863 XmaI C'CCGG_G Cuts at: 1870 2356 3014 1870 Size: 486 658 4545 Fragments arranged by size: 4545 658 486 XmnI GAAnn'nnTTC Cuts at: 2877 3818 2877 Size: 941 4748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI