Restriction map for
pFN21AB8952
(Circular) (Six-base) MAPSORT of: pFN21AB8952.seq Check: 8545 from: 1 to: 5686
pFN21AB8952 5686 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2611 3029 1937
Size: 674 418 4594
Fragments arranged by size:
4594 674 418
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2838 3749 5133 5401 1632
Size: 1206 911 1384 268 1917
Fragments arranged by size:
1917 1384 1206 911 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaI G_GGCC'C
Cuts at: 2098 2432 2098
Size: 334 5352
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2736 2785 2923 2995 3221 3373 5482 1493
Size: 1243 49 138 72 226 152 2109 1697
Fragments arranged by size:
2109 1697 1243 226 152 138 72 49
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2148 2274 2404 2425 2449 3011
Size: 73 136 69 126 130 21 24 562
Cuts at: 3011 1870
Size: 4545
Fragments arranged by size:
4545 562 136 130 126 73 69 24
21
AvrII C'CTAG_G
Cuts at: 2185 2185
Size: 5686
BamHI G'GATC_C
Cuts at: 2136 3016 3383 2136
Size: 880 367 4439
Fragments arranged by size:
4439 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2098 2393 2432 3005 1391
Size: 707 295 39 573 4072
Fragments arranged by size:
4072 707 573 295 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 4297 136
Size: 107 122 71 631 605 772 1853 1525
Fragments arranged by size:
1853 1525 772 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2155 2432 3747 4903 1790
Size: 308 57 277 1315 1156 2573
Fragments arranged by size:
2573 1315 1156 308 277 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2396 4346 1239
Size: 972 185 1950 2579
Fragments arranged by size:
2579 1950 972 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4349 882
Size: 617 2850 2219
Fragments arranged by size:
2850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2101 2131 1178
Size: 30 165 30 698 30 4733
Fragments arranged by size:
4733 698 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2262 2960 1186
Size: 1076 698 3912
Fragments arranged by size:
3912 1076 698
BseYI C'CCAG_C
Cuts at: 2214 2336 2843 4889 5385 2214
Size: 122 507 2046 496 2515
Fragments arranged by size:
2515 2046 507 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2369 2386 2400 2436 3136 3897
Size: 275 186 368 17 14 36 700 761
Cuts at: 3897 4925 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 700 368 275 186 36
17 14
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2020
Size: 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2155 2393 2432 3005 3747 3832
Size: 399 308 57 238 39 573 742 85
Cuts at: 3832 4903 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 573 399 308 238 85
57 39
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2409 3573 1394
Size: 429 215 371 1164 3507
Fragments arranged by size:
3507 1164 429 371 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2364 2399 2468 4330 4517 1700
Size: 110 554 35 69 1862 187 2869
Fragments arranged by size:
2869 1862 554 187 110 69 35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 2107 1184
Size: 923 4763
BstYI r'GATC_y
Cuts at: 1972 2136 3016 3383 3768 3785 5237 5323 5335
Size: 164 880 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 880 385 367 346 164 86
17 12
BstZ17I GTA'TAC
Cuts at: 2612 2612
Size: 5686
Bsu36I CC'TnA_GG
Cuts at: 2534 2534
Size: 5686
BtgI C'CryG_G
Cuts at: 513 1065 2112 2256 2511 2590 513
Size: 552 1047 144 255 79 3609
Fragments arranged by size:
3609 1047 552 255 144 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 3202 3996 4016 2153
Size: 1049 794 20 3823
Fragments arranged by size:
3823 1049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2550 2764 2990 2550
Size: 214 226 5246
Fragments arranged by size:
5246 226 214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2366 2383
Size: 54 1425 50 275 18 220 316 17
Cuts at: 2383 2433 2521 2593 3133 4022 5651 8
Size: 50 88 72 540 889 1629 43
Fragments arranged by size:
1629 1425 889 540 316 275 220 88
72 54 50 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2366 2383 2433 3133 1537
Size: 275 554 17 50 700 4090
Fragments arranged by size:
4090 700 554 275 50 17
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3616 1353
Size: 821 1442 3423
Fragments arranged by size:
3423 1442 821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 4273 4647 4793 387
Size: 1064 138 520 2164 374 146 1280
Fragments arranged by size:
2164 1280 1064 520 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2396 2838 3749 4346 5133 5401
Size: 393 579 185 442 911 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 911 787 597 579 442 393 268
185
EcoO109I rG'GnC_Cy
Cuts at: 2094 2095 2422 2094
Size: 1 327 5358
Fragments arranged by size:
5358 327 1
EcoRI G'AATT_C
Cuts at: 2736 2995 3373 2736
Size: 259 378 5049
Fragments arranged by size:
5049 378 259
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2285 4833 1678
Size: 607 2548 2531
Fragments arranged by size:
2548 2531 607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 4327 4359 4401
Size: 32 135 32 529 32 2194 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 2194 529 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2952 4800 4984 1305
Size: 1647 1848 184 2007
Fragments arranged by size:
2007 1848 1647 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2595 10
Size: 54 1425 343 763 3101
Fragments arranged by size:
3101 1425 763 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2619 3644 4003 4162 518
Size: 648 1453 1025 359 159 2042
Fragments arranged by size:
2042 1453 1025 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2332 2503 2513 3102 3779 4927 5172
Size: 423 303 171 10 589 677 1148 245
Cuts at: 5172 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 589 423 303 245 171
10
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 5686
NcoI C'CATG_G
Cuts at: 513 1065 2590 513
Size: 552 1525 3609
Fragments arranged by size:
3609 1525 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2139 2139
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2422 2422
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 2012 1613
Size: 399 5287
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspOMI G'GGCC_C
Cuts at: 2094 2428 2094
Size: 334 5352
PstI C_TGCA'G
Cuts at: 830 2461 3038 830
Size: 1631 577 3478
Fragments arranged by size:
3478 1631 577
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5686
SacII CC_GC'GG
Cuts at: 2514 2514
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2457 3034 4169 4850 5041 826
Size: 219 1412 577 1135 681 191 1471
Fragments arranged by size:
1471 1412 1135 681 577 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 2150 2427 3013 1872
Size: 278 277 586 4545
Fragments arranged by size:
4545 586 278 277
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2185 2442 2590 3125 513
Size: 552 1120 257 148 535 3074
Fragments arranged by size:
3074 1120 552 535 257 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 3713 3730 3883 4068 4513 325
Size: 1255 456 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 456 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2953 3932 5644
Size: 274 80 33 51 1443 976 979 1712
Cuts at: 5644 96
Size: 138
Fragments arranged by size:
1712 1443 979 976 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 2148 2425 3011 1870
Size: 278 277 586 4545
Fragments arranged by size:
4545 586 278 277
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI